Objective:Observe and analyze the clinical significance of the differences in the expression levels of sCD163,haptoglobin and cytokines.Methods:120 patients with tuberculous pleural effusion diagnosed in our hospital ...Objective:Observe and analyze the clinical significance of the differences in the expression levels of sCD163,haptoglobin and cytokines.Methods:120 patients with tuberculous pleural effusion diagnosed in our hospital from January 2019 to December 2021 were randomly selected as the experimental group.On the other hand,40 patients with non-tuberculous pleural effusion admitted in the same period were selected as the control group.The expression levels of sCD163,haptoglobin and cytokines were observed and analyzed.Results:The expression levels of sCD163(μg/mL),haptoglobin(g/L),IL-6(pg/mL)and IL-12(pg/mL)of the control group were 31.26±14.12,32.14±18.79,401.23±24.36 and 1.32±0.14,respectively.As for the experimental group,the expression levels of sCD163(μg/mL),haptoglobin(g/L),IL-6(pg/mL)and IL-12(pg/mL)were 74.12±14.78,113.25±19.45,612.12±36.98 and 4.12±0.56 respectively,and p<0.05 which shows that the data was statistically significant.Conclusion:The level of inflammatory cytokines in the pleural fluid of tuberculous pleural effusion patients are higher,which can be used for the diagnosis of auxiliary tuberculous pleurisy.Tuberculous pleural effusion patients has a significantly increased expression levels of sCD163 and haptoglobin in the pleural fluid.The combination of sCD163 and haptoglobin in the diagnosis of tuberculous pleural effusion has higher clinical diagnostic value,and sCD163 and haptoglobin are not interfered by inflammatory factors in the diagnosis of tuberculous pleural effusion。展开更多
CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-...CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (﹣1447A > G and ﹣135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the ﹣135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and ﹣135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at ﹣135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.展开更多
Objective:The inefficiency of conventional laboratory methods for diagnosis of Pleural tuberculosis(TBP) and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic strategies.Our goal w...Objective:The inefficiency of conventional laboratory methods for diagnosis of Pleural tuberculosis(TBP) and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic strategies.Our goal was to evaluate different laboratory techniques Ziehl Neelsen,Mantoux skin test,determination of interferon gamma in serum and pleural fluid,polymerase chain reaction and serological study of specific IgG,IgM and IgA beside bacteriological culture by BACTEC 460 TB for rapid and accurate diagnosis of tuberculosis pleurisy. Methods:Patients presented with pleural effusions were subjected to study by ZN,PCR,serological study By specific IgG,IgM and IgA for A60 of tuberculosis compared to culture by BACTEC460 TB.Interferon gamma was determined both in serum and pleural fluid.Results:Mantoux skin test was positive in 19 TBP patients and four patients with exudative pleural effusion.Z.N staining results of pleural biopsy specimens were positive in only 1 of 23 patients(4.3%) in the tuberculous pleural effusion group.PCR was positive in 20 cases of group 1(87%).Serum and pleural fluid interferon had significantly elevated levels(P【0.000 1) in TBP and both measurements had significant correlation in TBP(P【0.000 1).The serum IgA ELISA test was positive in 7/23(30.4%),IgM was positive in 17/23(73.9%) patients and IgG was positive in 16/23(69%) patients.Non of the non TBP had either ZN,PCR or positive serum IgA,IgG,or IgM.When the positive results for IgG and IgM were combined together the serological tests correcdy identified 20/23(87%) of patients. Conclusion:We suggest that in TBP serological diagnosis by combined use of IgG and IgM for A60 antigen with serum determination of interferon gamma can provide rapid and non invasive diagnostic tool that can justify the starting of chemotherapy while awaiting the results of culture.展开更多
目的探讨纤维蛋白原/白蛋白比值(fibrinogen to albumin ratio,FAR)与结核性胸膜炎患者发生胸膜粘连的关系及其对胸膜粘连严重程度的预测价值。方法收集2019年9月至2021年12月在我院行胸腔镜手术确诊结核性胸膜炎的111名患者的临床资料...目的探讨纤维蛋白原/白蛋白比值(fibrinogen to albumin ratio,FAR)与结核性胸膜炎患者发生胸膜粘连的关系及其对胸膜粘连严重程度的预测价值。方法收集2019年9月至2021年12月在我院行胸腔镜手术确诊结核性胸膜炎的111名患者的临床资料。住院期间测定其血清ADA、LDH、Alb、CRP和Fib。探讨其临床资料及实验室检测与胸膜粘连的相关性。结果有胸膜粘连的患者病程更长,血清ADA含量、CRP、Fib、FAR均更高,而Alb水平降低,且上述指标与胸膜粘连程度均有相关性,其中FAR的相关性最强(Rho=0.349,P<0.001)。在预测结核性胸膜炎患者发生胸膜粘连方面,FAR的预测价值最高(AUC=0.754,95%CI 0.661~0.848,P<0.001),当其最佳临界值为14.55时,预测胸膜粘连的敏感度为70.5%,特异度为87.9%;在有胸膜粘连的患者中,FAR>14.55的患者更易发生重度胸膜粘连。结论FAR可用于预测结核性胸膜炎患者胸膜粘连程度,对于高FAR的患者,应早期干预,早期治疗。展开更多
文摘Objective:Observe and analyze the clinical significance of the differences in the expression levels of sCD163,haptoglobin and cytokines.Methods:120 patients with tuberculous pleural effusion diagnosed in our hospital from January 2019 to December 2021 were randomly selected as the experimental group.On the other hand,40 patients with non-tuberculous pleural effusion admitted in the same period were selected as the control group.The expression levels of sCD163,haptoglobin and cytokines were observed and analyzed.Results:The expression levels of sCD163(μg/mL),haptoglobin(g/L),IL-6(pg/mL)and IL-12(pg/mL)of the control group were 31.26±14.12,32.14±18.79,401.23±24.36 and 1.32±0.14,respectively.As for the experimental group,the expression levels of sCD163(μg/mL),haptoglobin(g/L),IL-6(pg/mL)and IL-12(pg/mL)were 74.12±14.78,113.25±19.45,612.12±36.98 and 4.12±0.56 respectively,and p<0.05 which shows that the data was statistically significant.Conclusion:The level of inflammatory cytokines in the pleural fluid of tuberculous pleural effusion patients are higher,which can be used for the diagnosis of auxiliary tuberculous pleurisy.Tuberculous pleural effusion patients has a significantly increased expression levels of sCD163 and haptoglobin in the pleural fluid.The combination of sCD163 and haptoglobin in the diagnosis of tuberculous pleural effusion has higher clinical diagnostic value,and sCD163 and haptoglobin are not interfered by inflammatory factors in the diagnosis of tuberculous pleural effusion。
文摘CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (﹣1447A > G and ﹣135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the ﹣135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and ﹣135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at ﹣135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.
文摘Objective:The inefficiency of conventional laboratory methods for diagnosis of Pleural tuberculosis(TBP) and the reliance on pleural biopsy have motivated the evaluation of alternative diagnostic strategies.Our goal was to evaluate different laboratory techniques Ziehl Neelsen,Mantoux skin test,determination of interferon gamma in serum and pleural fluid,polymerase chain reaction and serological study of specific IgG,IgM and IgA beside bacteriological culture by BACTEC 460 TB for rapid and accurate diagnosis of tuberculosis pleurisy. Methods:Patients presented with pleural effusions were subjected to study by ZN,PCR,serological study By specific IgG,IgM and IgA for A60 of tuberculosis compared to culture by BACTEC460 TB.Interferon gamma was determined both in serum and pleural fluid.Results:Mantoux skin test was positive in 19 TBP patients and four patients with exudative pleural effusion.Z.N staining results of pleural biopsy specimens were positive in only 1 of 23 patients(4.3%) in the tuberculous pleural effusion group.PCR was positive in 20 cases of group 1(87%).Serum and pleural fluid interferon had significantly elevated levels(P【0.000 1) in TBP and both measurements had significant correlation in TBP(P【0.000 1).The serum IgA ELISA test was positive in 7/23(30.4%),IgM was positive in 17/23(73.9%) patients and IgG was positive in 16/23(69%) patients.Non of the non TBP had either ZN,PCR or positive serum IgA,IgG,or IgM.When the positive results for IgG and IgM were combined together the serological tests correcdy identified 20/23(87%) of patients. Conclusion:We suggest that in TBP serological diagnosis by combined use of IgG and IgM for A60 antigen with serum determination of interferon gamma can provide rapid and non invasive diagnostic tool that can justify the starting of chemotherapy while awaiting the results of culture.
文摘目的探讨纤维蛋白原/白蛋白比值(fibrinogen to albumin ratio,FAR)与结核性胸膜炎患者发生胸膜粘连的关系及其对胸膜粘连严重程度的预测价值。方法收集2019年9月至2021年12月在我院行胸腔镜手术确诊结核性胸膜炎的111名患者的临床资料。住院期间测定其血清ADA、LDH、Alb、CRP和Fib。探讨其临床资料及实验室检测与胸膜粘连的相关性。结果有胸膜粘连的患者病程更长,血清ADA含量、CRP、Fib、FAR均更高,而Alb水平降低,且上述指标与胸膜粘连程度均有相关性,其中FAR的相关性最强(Rho=0.349,P<0.001)。在预测结核性胸膜炎患者发生胸膜粘连方面,FAR的预测价值最高(AUC=0.754,95%CI 0.661~0.848,P<0.001),当其最佳临界值为14.55时,预测胸膜粘连的敏感度为70.5%,特异度为87.9%;在有胸膜粘连的患者中,FAR>14.55的患者更易发生重度胸膜粘连。结论FAR可用于预测结核性胸膜炎患者胸膜粘连程度,对于高FAR的患者,应早期干预,早期治疗。
