We conducted the first light curve study of the binary star EL Tuc within the Binary Systems of South and North project's framework.The photometric observations were made using standard multiband BVR_cI_c filters ...We conducted the first light curve study of the binary star EL Tuc within the Binary Systems of South and North project's framework.The photometric observations were made using standard multiband BVR_cI_c filters at an observatory in Argentina.We presented a new ephemeris for EL Tuc and a linear fit to the O–C diagram,utilizing our extracted times of minima and additional literature.We employed the PHysics Of Eclipsing BinariEs Python code and the Markov chain Monte Carlo approach for the system's light curve analysis.The target system's light curve solution required a cold starspot on the hotter component.We conclude that EL Tuc is a total contact binary system with a low mass ratio of q=0.172±0.002,an orbital inclination of i=83°.74±0°.40,and a fillout factor of f=53.7%±1.6%.We used the P-a relationship and the Gaia Data Release 3 parallax method to determine the absolute parameters of EL Tuc to compare the precision of our results.This system was classified as W-type based on the mass and effective temperature of the companion stars.The positions of the systems were depicted on the M-L,M-R,T-M,and q-Lratiodiagrams.The relationship between the spectroscopic and photometric mass ratios of binaries was discussed.展开更多
目的:探讨应用四环素调控长链非编码RNA TUC338在膀胱癌细胞内的表达及抗肿瘤作用。方法:将针对TUC338的sh RNA序列插入到四环素诱导系统的下端,构建四环素诱导的TUC338 sh RNA并转染细胞。应用实时荧光定量PCR检测细胞内TUC338的表达水...目的:探讨应用四环素调控长链非编码RNA TUC338在膀胱癌细胞内的表达及抗肿瘤作用。方法:将针对TUC338的sh RNA序列插入到四环素诱导系统的下端,构建四环素诱导的TUC338 sh RNA并转染细胞。应用实时荧光定量PCR检测细胞内TUC338的表达水平,采用CCK8和半胱天冬酶3酶联免疫吸附测定法的方法分别检测细胞的增殖和凋亡。结果:四环素诱导的TUC338 sh RNA可以降低TUC338的表达,抑制膀胱癌细胞增殖和促进膀胱癌细胞的凋亡。结论:通过构建四环素基因表达调控系统,可精确控制TUC338在膀胱癌细胞内表达,并发挥杀伤肿瘤细胞功能,为进一步构建特异、高效的膀胱癌合成生物治疗器件提供理论基础。展开更多
文摘We conducted the first light curve study of the binary star EL Tuc within the Binary Systems of South and North project's framework.The photometric observations were made using standard multiband BVR_cI_c filters at an observatory in Argentina.We presented a new ephemeris for EL Tuc and a linear fit to the O–C diagram,utilizing our extracted times of minima and additional literature.We employed the PHysics Of Eclipsing BinariEs Python code and the Markov chain Monte Carlo approach for the system's light curve analysis.The target system's light curve solution required a cold starspot on the hotter component.We conclude that EL Tuc is a total contact binary system with a low mass ratio of q=0.172±0.002,an orbital inclination of i=83°.74±0°.40,and a fillout factor of f=53.7%±1.6%.We used the P-a relationship and the Gaia Data Release 3 parallax method to determine the absolute parameters of EL Tuc to compare the precision of our results.This system was classified as W-type based on the mass and effective temperature of the companion stars.The positions of the systems were depicted on the M-L,M-R,T-M,and q-Lratiodiagrams.The relationship between the spectroscopic and photometric mass ratios of binaries was discussed.
文摘目的:探讨膀胱癌中长链非编码RNA(lnc RNA)TUC338的表达及作用机制。方法 :收集安徽医科大学一附院收治的30例膀胱癌手术患者的癌变组织和癌旁组织,通过实时定量PCR检测TUC338的表达水平并分析其临床病理特征之间的关联。运用RNA干扰(si-TUC338)的方法检测敲减TUC338,采用CCK8和半胱天冬酶3酶联免疫吸附测定法的方法分别检测TUC338对膀胱癌细胞的增殖、凋亡的影响。结果:相对于膀胱癌旁组织,TUC338 m RNA在膀胱癌组织中高表达。敲减TUC338后,细胞增殖被抑制,细胞凋亡增加。结论:TUC338是一个潜在的治疗膀胱癌的药物作用靶点。
文摘目的:探讨应用四环素调控长链非编码RNA TUC338在膀胱癌细胞内的表达及抗肿瘤作用。方法:将针对TUC338的sh RNA序列插入到四环素诱导系统的下端,构建四环素诱导的TUC338 sh RNA并转染细胞。应用实时荧光定量PCR检测细胞内TUC338的表达水平,采用CCK8和半胱天冬酶3酶联免疫吸附测定法的方法分别检测细胞的增殖和凋亡。结果:四环素诱导的TUC338 sh RNA可以降低TUC338的表达,抑制膀胱癌细胞增殖和促进膀胱癌细胞的凋亡。结论:通过构建四环素基因表达调控系统,可精确控制TUC338在膀胱癌细胞内表达,并发挥杀伤肿瘤细胞功能,为进一步构建特异、高效的膀胱癌合成生物治疗器件提供理论基础。
