Aberrant biological information occurs naturally at exposure to bisphenol A or its alternatives,which was associated with the occurrence and development of breast cancer.However,the potential molecular variation in ge...Aberrant biological information occurs naturally at exposure to bisphenol A or its alternatives,which was associated with the occurrence and development of breast cancer.However,the potential molecular variation in gene expression during the breast tumor development is still unclear.Herein,high throughput RNA sequencing(RNA-Seq)and bioinformatics analysis were used to investigate the variation of tumor-mRNA profile exposed with BPS5(5µg/kg bw/day)or BPS50(50µg/kg bw/day)in tumor development-associated MMTV-PyMT transgenic mouse model.Meanwhile,we analyzed the dose-effects of bisphenol S(BPS)and BPS-induced tumor development on the gene level exhaustively.In dose-effect aspects of BPS,the increased concentration of BPS significantly changed the numbers and enrichment pathway of differentially expressed genes(DEGs),especially the enrichment pathways involved in up-regulated genes including ribosome,peroxisome proliferators-activated receptor(PPAR)signaling pathway and progesterone-mediated oocyte maturation pathway.In effects of BPS exposure to tumor development,expression of IgκC,Zfp385b,Cldn10,Pgr and Snord14d has changed significantly throughout the tumor development.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)results obtained from BPS-induced tumor development showed that the functional classifications were intensively altered with an extension of time in high-dose BPS groups.The acquired DEGs and pathway information could help with the accurate exploration of molecular mechanisms of tumor development,screening of molecular targets of breast cancer,and toxicological evaluation of environmental pollutants.展开更多
Transforming growth factor-β(TGF-β) signaling regulates cell proliferation, differentiation, migration and death, and plays a critical role in embryogenesis and tissue homeostasis. Its deregulation results in variou...Transforming growth factor-β(TGF-β) signaling regulates cell proliferation, differentiation, migration and death, and plays a critical role in embryogenesis and tissue homeostasis. Its deregulation results in various diseases including tumor formation.Receptor tyrosine kinases(RTKs), such as epidermal growth factor receptor(EGFR), fibroblast growth factor receptor(FGFR),vascular endothelial growth factor receptor(VEGFR) and platelet-derived growth factor receptor(PDGFR), also play key roles in the development and progression of many types of tumors. It has been realized that TGF-β signaling and RTK pathways interact with each other and their interplay is important for cancer development. They are mutually regulated and cooperatively modulate cell survival and migration, epithelial-mesenchymal transition, and tumor microenvironment to accelerate tumorigenesis and tumor metastasis. RTKs can modulate Smad-dependent transcription or cooperate with TGF-β to potentiate its oncogenic activity,while TGF-β signaling can in turn control RTK signaling by regulating their activities or expression. This review summarizes current understandings of the interplay between TGF-β signaling and RTKs and its influence on tumor development.展开更多
Many digestive system malignant tumors are characterized by high incidence and mortality rate.Increasing evidence has revealed that the tumor microenvironment(TME)is involved in cancer initiation and tumor progression...Many digestive system malignant tumors are characterized by high incidence and mortality rate.Increasing evidence has revealed that the tumor microenvironment(TME)is involved in cancer initiation and tumor progression.Tumor-associated macrophages(TAMs)are a predominant constituent of the TME,and participate in the regulation of various biological behaviors and influence the prognosis of digestive system cancer.TAMs can be mainly classified into the antitumor M1 phenotype and protumor M2 phenotype.The latter especially are crucial drivers of tumor invasion,growth,angiogenesis,metastasis,immunosuppression,and resistance to therapy.TAMs are of importance in the occurrence,development,diagnosis,prognosis,and treatment of common digestive system malignant tumors.In this review,we summarize the role of TAMs in common digestive system malignant tumors,including esophageal,gastric,colorectal,pancreatic and liver cancers.How TAMs promote the development of tumors,and how they act as potential therapeutic targets and their clinical applications are also described.展开更多
This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of v...This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of various developmental toxicants. The results showed that 2 of 3 developmental toxicants under consideration, sodium pentobarbital and ethanol, significantly inhibited S180cells attachment to Concanavalin A-coaed surfaces. Inhibition was dependent on concentration, and the IC50 (the concentration tha reduced attachment by 50% ), of these 2 chemicals was 1.2×10-3mol/L and 1 .0 mol/L, respectively. Anoher developmental toxiant, hydmiortisone, did not show inhibitory activity. Two non-developmental toxicants, sodium chloride and glycine were also tested and these did not decrease attachment rates. The main results reported here were generally sindlar to those obtained with ascitic mouse ovdrian tumor cells as a model. Therefore, this study added further evidence to the conclusion that cell specificity does not lindt attachment inhibition to Con A-coated surfaces, so S180 cell may serve as an altemative cell model, especially when other cell lines are unavailable. Furthermore, after optimal validation, it can be suggested that an S180 cell attachment assay may be a candidate for a series of assays to detect developmental toxicants.展开更多
Brain tumors are a diverse group of malignancies that remain refractory to conventional treatment approaches.Molecular neuro-oncologyhas now begun to clarify the transformed phenotype of brain tumors and identify onco...Brain tumors are a diverse group of malignancies that remain refractory to conventional treatment approaches.Molecular neuro-oncologyhas now begun to clarify the transformed phenotype of brain tumors and identify oncogenic pathways that might be amenable to targetedtherapy.Activity of the phosphoinositide 3;kinase(PI3K)/Akt pathway is often upregulated in brain tumors due to excessive stimu-lation by growth factor receptors and Ras.Loss of function of the tumor suppressor gene PTEN also frequently contributesto展开更多
Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiatio...Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.展开更多
Malignant tumor has become a major threat affecting human health,and is one of the main causes of human death.Recent studies have shown that many traditional Chinese medicines(TCM)have good anti-tumor activity,which m...Malignant tumor has become a major threat affecting human health,and is one of the main causes of human death.Recent studies have shown that many traditional Chinese medicines(TCM)have good anti-tumor activity,which may improve the therapeutic effect of routine treatment and quality of life with lower toxicity.However,the efficacy of TCM alone for the treatment of tumors is limited.Metal ions are essential substances for maintaining normal physiological activities.This article summarized the multiple mechanisms in which metal ions are involved in the prevention and treatment of tumors in TCM.展开更多
Malignant tumor is still a major problem worldwide.During tumorigenesis or tumor development,tumor suppressor p53-binding protein 2(TP53BP2),also known as apoptosis stimulating protein 2 of p53(ASPP2),plays a critical...Malignant tumor is still a major problem worldwide.During tumorigenesis or tumor development,tumor suppressor p53-binding protein 2(TP53BP2),also known as apoptosis stimulating protein 2 of p53(ASPP2),plays a critical role in p53 dependent and independent manner.Expression of TP53BP2 is highly correlated with the prognosis and survival rate of malignant tumor patients.TP53BP2 can interact with p53,NF-κB p65,Bcl-2,HCV core protein,PP1,YAP,CagA,RAS,PAR3,and other proteins to regulate cell function.Moreover,TP53BP2 can also regulate the proliferation,apoptosis,autophagy,migration,EMT and drug resistance of tumor cells through downstream signaling pathways,such as NF-κB,RAS/MAPK,mevalonate,TGF-β1,PI3K/AKT,aPKC-ι/GLI1 and autophagy pathways.As a potential therapeutic target,TP53BP2 has been attracted more attention.We review the role of TP53BP2 in tumorigenesis or tumor development and the signal pathway involved in TP53BP2,which may provide more deep insight and strategies for tumor treatment.展开更多
使用TALEN技术制作p53基因敲除大鼠模型,建立种群并分析大鼠表型。设计特异性识别p53基因外显子2的TALEN蛋白并构建相应载体,将体外转录的TALEN m RNA注射SD大鼠受精卵,出生后从仔鼠中通过测序筛选靶位点正确剪切的阳性小鼠。结果显示,...使用TALEN技术制作p53基因敲除大鼠模型,建立种群并分析大鼠表型。设计特异性识别p53基因外显子2的TALEN蛋白并构建相应载体,将体外转录的TALEN m RNA注射SD大鼠受精卵,出生后从仔鼠中通过测序筛选靶位点正确剪切的阳性小鼠。结果显示,注射得到11只新生仔鼠,通过测序发现其中有10只靶位点发生剪切。选取其中4只作为首建鼠进行繁殖。p53基因敲除纯合子表现出肿瘤易发性,主要自发性肿瘤类型为恶性纤维组织肉瘤。此外,p53基因敲除纯合子大鼠表现出眼睛发育异常,视网膜变性及晶状体纤维排列紊乱。通过TALEN技术高效地获得了p53基因敲除大鼠模型,纯合子敲除大鼠除了易发肿瘤并伴有眼发育异常,因而该敲除大鼠模型除了可用于研究肿瘤发生机制外,也可用于研究p53基因在眼发育过程中的功能。展开更多
目的:观察石菖蒲配合针刺疗法对脑瘫患儿的康复治疗作用。方法:石菖蒲联合针刺治疗组(52例),治疗组采用内服煎汤提取液和头针、体针,对照组(52例)单纯用针刺治疗,观察评估患儿的发育商(developmental quotient,DQ),头颅CT检测,采用粗大...目的:观察石菖蒲配合针刺疗法对脑瘫患儿的康复治疗作用。方法:石菖蒲联合针刺治疗组(52例),治疗组采用内服煎汤提取液和头针、体针,对照组(52例)单纯用针刺治疗,观察评估患儿的发育商(developmental quotient,DQ),头颅CT检测,采用粗大运动功能测试量表(gross motor function measure,GMFM)评分,采用酶联免疫吸附法测定肿瘤坏死因子-α(TNF-α)的含量。结果:治疗后,治疗组脑血液动力学指标显著改善(P<0.05)。治疗组CT结果显示恢复率较显著增高(P<0.05)。治疗组治疗后DQ显著提高(P<0.05),治疗组GMFM评估总分显著提高(P<0.05)。联合治疗组血清TNF-α水平显著降低(P<0.05)。结论:石菖蒲发挥其通关复苏、益智健脑的功效,能够加强针刺治疗的效果,通过降低炎症反应和肿瘤坏死因子-α(TNF-α)的表达,减缓脑瘫带来的脑损伤,改善肌张力和肌力,对脑瘫患儿起到较好的康复治疗作用。展开更多
Primary hepatic leiomyoma is a neoplasm of mesen-chymal origin and occurs only rarely. Secondary to benign smooth muscle proliferation, it is usually found in adult women and is associated with Epstein-Barr virus (EBV...Primary hepatic leiomyoma is a neoplasm of mesen-chymal origin and occurs only rarely. Secondary to benign smooth muscle proliferation, it is usually found in adult women and is associated with Epstein-Barr virus (EBV) infection. Here, we report the 29 th case of primary hepatic leiomyoma with its unique features related to diagnosis, treatment and developmental biology. A 48-year-old man, with an immunocompromised status, complained of pain in the upper quadrant of the abdomen. Serological analysis indicated no presence of hepatitis virus, no human immunodeficiency virus, and no EBV infection. The levels of α-fetoproteinand carcinoembryonic antigen were normal. A mass was detected in segment Ⅲ of the hepatic lobe by ultrasonography and an abdominal computed tomography scan. Endoscopy had negative findings. Exploratory laparotomy found no existing extrahepatic tumor and left lateral lobectomy was performed. Pathological examination showed the mass to be a typical leiomyoma. The cells were positive for α-smooth muscle actin and desmin, and negative for the makers of gastrointestinal stromal tumor (GIST), including CD117, CD34 and DOG1 (discovered on GIST1). In situ hybridization revealed negative status for EBV-encoded small RNA. After left lateral lobectomy, the patient was not given chemotherapy or radiotherapy. During a 2-year follow- up, no sign of local recurrence or distant metastasis was observed. In conclusion, we report a rare case of primary hepatic leiomyoma in a male patient without EBV infection. Hepatic resection was curative. This case presents data to expand our knowledge concerning the complex and heterogeneous nature of primary liver leiomyoma, indicating that EBV infection is important but neither necessary nor sufficient for the development of primary liver leiomyoma.展开更多
背景:因骨盆解剖结构较为复杂,与多个重要脏器、血管、神经毗邻,通过传统方法治疗复杂髋关节疾病的操作难度较大。利用3D打印技术显著提升了此类疾病的手术成功率及患者满意度。目的:归纳3D打印技术在髋关节疾病治疗中的应用效果。方法...背景:因骨盆解剖结构较为复杂,与多个重要脏器、血管、神经毗邻,通过传统方法治疗复杂髋关节疾病的操作难度较大。利用3D打印技术显著提升了此类疾病的手术成功率及患者满意度。目的:归纳3D打印技术在髋关节疾病治疗中的应用效果。方法:利用计算机检索CNKI、PubMed数据库2000年1月至2019年3月发表的相关文献,中文检索词为“3D打印技术,发育性髋关节发育不良,骨盆骨折,髋臼骨折,骨盆肿瘤,髋臼肿瘤、髋关节置换”,英文检索词为“3D printing,developmental dysplasia of the hip,DDH,pelvic fracture,acetabular fracture,pelvic tumor,acetabular tumor,replacement or the hip joint”。共检索到103篇文章,根据纳入与排除标准最终纳入41篇文献进行综述。结果与结论:3D打印的优势在于不需要模具,直接通过数字化技术制造复杂的几何模具或模型。将3D打印技术应用在发育性髋关节发育不良、复杂的骨盆及髋臼骨折与髋关节肿瘤的临床治疗中,显著提高了患者满意度,减少了并发症的发生。因此,将3D打印技术用于髋关节疾病的诊治对髋关节疾病的术前指导、术中操作以及术后康复都具有重要临床意义与广阔的应用前景。展开更多
基金supported by the National Natural Science Foundation of China(No.22176195)the Natural Science Foundation of Guangdong Province,China(No.2021A1515010171)+4 种基金the Key Program of Fundamental Research in Shenzhen,China(NoJCYJ20210324115811031)the Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression,China(No.ZDSYS20220606100606014)the Shenzhen Key Medical Discipline Construction Fund,China(No.SZXK052)the Clinical Research Project of Shenzhen Second People’s Hospital,China(No.20203357001)the Guangdong Basic and Applied Basic Research Foundation,China(No.2021A1515110096).
文摘Aberrant biological information occurs naturally at exposure to bisphenol A or its alternatives,which was associated with the occurrence and development of breast cancer.However,the potential molecular variation in gene expression during the breast tumor development is still unclear.Herein,high throughput RNA sequencing(RNA-Seq)and bioinformatics analysis were used to investigate the variation of tumor-mRNA profile exposed with BPS5(5µg/kg bw/day)or BPS50(50µg/kg bw/day)in tumor development-associated MMTV-PyMT transgenic mouse model.Meanwhile,we analyzed the dose-effects of bisphenol S(BPS)and BPS-induced tumor development on the gene level exhaustively.In dose-effect aspects of BPS,the increased concentration of BPS significantly changed the numbers and enrichment pathway of differentially expressed genes(DEGs),especially the enrichment pathways involved in up-regulated genes including ribosome,peroxisome proliferators-activated receptor(PPAR)signaling pathway and progesterone-mediated oocyte maturation pathway.In effects of BPS exposure to tumor development,expression of IgκC,Zfp385b,Cldn10,Pgr and Snord14d has changed significantly throughout the tumor development.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)results obtained from BPS-induced tumor development showed that the functional classifications were intensively altered with an extension of time in high-dose BPS groups.The acquired DEGs and pathway information could help with the accurate exploration of molecular mechanisms of tumor development,screening of molecular targets of breast cancer,and toxicological evaluation of environmental pollutants.
文摘Transforming growth factor-β(TGF-β) signaling regulates cell proliferation, differentiation, migration and death, and plays a critical role in embryogenesis and tissue homeostasis. Its deregulation results in various diseases including tumor formation.Receptor tyrosine kinases(RTKs), such as epidermal growth factor receptor(EGFR), fibroblast growth factor receptor(FGFR),vascular endothelial growth factor receptor(VEGFR) and platelet-derived growth factor receptor(PDGFR), also play key roles in the development and progression of many types of tumors. It has been realized that TGF-β signaling and RTK pathways interact with each other and their interplay is important for cancer development. They are mutually regulated and cooperatively modulate cell survival and migration, epithelial-mesenchymal transition, and tumor microenvironment to accelerate tumorigenesis and tumor metastasis. RTKs can modulate Smad-dependent transcription or cooperate with TGF-β to potentiate its oncogenic activity,while TGF-β signaling can in turn control RTK signaling by regulating their activities or expression. This review summarizes current understandings of the interplay between TGF-β signaling and RTKs and its influence on tumor development.
基金Supported by National Natural Science Foundation of China,No.82272396Suzhou Medical and Health Science and Technology Innovation Project,No.SKY2022057The Youth Medical Talent of Jiangsu Province,No.QNRC2016475.
文摘Many digestive system malignant tumors are characterized by high incidence and mortality rate.Increasing evidence has revealed that the tumor microenvironment(TME)is involved in cancer initiation and tumor progression.Tumor-associated macrophages(TAMs)are a predominant constituent of the TME,and participate in the regulation of various biological behaviors and influence the prognosis of digestive system cancer.TAMs can be mainly classified into the antitumor M1 phenotype and protumor M2 phenotype.The latter especially are crucial drivers of tumor invasion,growth,angiogenesis,metastasis,immunosuppression,and resistance to therapy.TAMs are of importance in the occurrence,development,diagnosis,prognosis,and treatment of common digestive system malignant tumors.In this review,we summarize the role of TAMs in common digestive system malignant tumors,including esophageal,gastric,colorectal,pancreatic and liver cancers.How TAMs promote the development of tumors,and how they act as potential therapeutic targets and their clinical applications are also described.
文摘This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of various developmental toxicants. The results showed that 2 of 3 developmental toxicants under consideration, sodium pentobarbital and ethanol, significantly inhibited S180cells attachment to Concanavalin A-coaed surfaces. Inhibition was dependent on concentration, and the IC50 (the concentration tha reduced attachment by 50% ), of these 2 chemicals was 1.2×10-3mol/L and 1 .0 mol/L, respectively. Anoher developmental toxiant, hydmiortisone, did not show inhibitory activity. Two non-developmental toxicants, sodium chloride and glycine were also tested and these did not decrease attachment rates. The main results reported here were generally sindlar to those obtained with ascitic mouse ovdrian tumor cells as a model. Therefore, this study added further evidence to the conclusion that cell specificity does not lindt attachment inhibition to Con A-coated surfaces, so S180 cell may serve as an altemative cell model, especially when other cell lines are unavailable. Furthermore, after optimal validation, it can be suggested that an S180 cell attachment assay may be a candidate for a series of assays to detect developmental toxicants.
文摘Brain tumors are a diverse group of malignancies that remain refractory to conventional treatment approaches.Molecular neuro-oncologyhas now begun to clarify the transformed phenotype of brain tumors and identify oncogenic pathways that might be amenable to targetedtherapy.Activity of the phosphoinositide 3;kinase(PI3K)/Akt pathway is often upregulated in brain tumors due to excessive stimu-lation by growth factor receptors and Ras.Loss of function of the tumor suppressor gene PTEN also frequently contributesto
文摘Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.
文摘Malignant tumor has become a major threat affecting human health,and is one of the main causes of human death.Recent studies have shown that many traditional Chinese medicines(TCM)have good anti-tumor activity,which may improve the therapeutic effect of routine treatment and quality of life with lower toxicity.However,the efficacy of TCM alone for the treatment of tumors is limited.Metal ions are essential substances for maintaining normal physiological activities.This article summarized the multiple mechanisms in which metal ions are involved in the prevention and treatment of tumors in TCM.
基金supported by Beijing Municipal Natural Science Foundation(China)(No.7192084)The Capital Health Research and Development of Special(China)(No.2020-2-1152)+2 种基金Beijing Municipal Institute of Public Medical Research Development and Reform Pilot Project(China)(No.Jingyiyan 2019-6)National Natural Science Foundation of China(No.81672026)Research and demonstration application of clinical diagnosis and treatment technology in the capital(China)(No.Z191100006619064).
文摘Malignant tumor is still a major problem worldwide.During tumorigenesis or tumor development,tumor suppressor p53-binding protein 2(TP53BP2),also known as apoptosis stimulating protein 2 of p53(ASPP2),plays a critical role in p53 dependent and independent manner.Expression of TP53BP2 is highly correlated with the prognosis and survival rate of malignant tumor patients.TP53BP2 can interact with p53,NF-κB p65,Bcl-2,HCV core protein,PP1,YAP,CagA,RAS,PAR3,and other proteins to regulate cell function.Moreover,TP53BP2 can also regulate the proliferation,apoptosis,autophagy,migration,EMT and drug resistance of tumor cells through downstream signaling pathways,such as NF-κB,RAS/MAPK,mevalonate,TGF-β1,PI3K/AKT,aPKC-ι/GLI1 and autophagy pathways.As a potential therapeutic target,TP53BP2 has been attracted more attention.We review the role of TP53BP2 in tumorigenesis or tumor development and the signal pathway involved in TP53BP2,which may provide more deep insight and strategies for tumor treatment.
文摘使用TALEN技术制作p53基因敲除大鼠模型,建立种群并分析大鼠表型。设计特异性识别p53基因外显子2的TALEN蛋白并构建相应载体,将体外转录的TALEN m RNA注射SD大鼠受精卵,出生后从仔鼠中通过测序筛选靶位点正确剪切的阳性小鼠。结果显示,注射得到11只新生仔鼠,通过测序发现其中有10只靶位点发生剪切。选取其中4只作为首建鼠进行繁殖。p53基因敲除纯合子表现出肿瘤易发性,主要自发性肿瘤类型为恶性纤维组织肉瘤。此外,p53基因敲除纯合子大鼠表现出眼睛发育异常,视网膜变性及晶状体纤维排列紊乱。通过TALEN技术高效地获得了p53基因敲除大鼠模型,纯合子敲除大鼠除了易发肿瘤并伴有眼发育异常,因而该敲除大鼠模型除了可用于研究肿瘤发生机制外,也可用于研究p53基因在眼发育过程中的功能。
文摘目的:观察石菖蒲配合针刺疗法对脑瘫患儿的康复治疗作用。方法:石菖蒲联合针刺治疗组(52例),治疗组采用内服煎汤提取液和头针、体针,对照组(52例)单纯用针刺治疗,观察评估患儿的发育商(developmental quotient,DQ),头颅CT检测,采用粗大运动功能测试量表(gross motor function measure,GMFM)评分,采用酶联免疫吸附法测定肿瘤坏死因子-α(TNF-α)的含量。结果:治疗后,治疗组脑血液动力学指标显著改善(P<0.05)。治疗组CT结果显示恢复率较显著增高(P<0.05)。治疗组治疗后DQ显著提高(P<0.05),治疗组GMFM评估总分显著提高(P<0.05)。联合治疗组血清TNF-α水平显著降低(P<0.05)。结论:石菖蒲发挥其通关复苏、益智健脑的功效,能够加强针刺治疗的效果,通过降低炎症反应和肿瘤坏死因子-α(TNF-α)的表达,减缓脑瘫带来的脑损伤,改善肌张力和肌力,对脑瘫患儿起到较好的康复治疗作用。
基金Supported by Grants from the National Natural Science Foundation of China,No.81072441,to Gong NGgrants from the National High-Tech Research and Development Program(Program 863)of the Ministry of Science and Technology of China,2012AA021010,to Ming CS
文摘Primary hepatic leiomyoma is a neoplasm of mesen-chymal origin and occurs only rarely. Secondary to benign smooth muscle proliferation, it is usually found in adult women and is associated with Epstein-Barr virus (EBV) infection. Here, we report the 29 th case of primary hepatic leiomyoma with its unique features related to diagnosis, treatment and developmental biology. A 48-year-old man, with an immunocompromised status, complained of pain in the upper quadrant of the abdomen. Serological analysis indicated no presence of hepatitis virus, no human immunodeficiency virus, and no EBV infection. The levels of α-fetoproteinand carcinoembryonic antigen were normal. A mass was detected in segment Ⅲ of the hepatic lobe by ultrasonography and an abdominal computed tomography scan. Endoscopy had negative findings. Exploratory laparotomy found no existing extrahepatic tumor and left lateral lobectomy was performed. Pathological examination showed the mass to be a typical leiomyoma. The cells were positive for α-smooth muscle actin and desmin, and negative for the makers of gastrointestinal stromal tumor (GIST), including CD117, CD34 and DOG1 (discovered on GIST1). In situ hybridization revealed negative status for EBV-encoded small RNA. After left lateral lobectomy, the patient was not given chemotherapy or radiotherapy. During a 2-year follow- up, no sign of local recurrence or distant metastasis was observed. In conclusion, we report a rare case of primary hepatic leiomyoma in a male patient without EBV infection. Hepatic resection was curative. This case presents data to expand our knowledge concerning the complex and heterogeneous nature of primary liver leiomyoma, indicating that EBV infection is important but neither necessary nor sufficient for the development of primary liver leiomyoma.
文摘背景:因骨盆解剖结构较为复杂,与多个重要脏器、血管、神经毗邻,通过传统方法治疗复杂髋关节疾病的操作难度较大。利用3D打印技术显著提升了此类疾病的手术成功率及患者满意度。目的:归纳3D打印技术在髋关节疾病治疗中的应用效果。方法:利用计算机检索CNKI、PubMed数据库2000年1月至2019年3月发表的相关文献,中文检索词为“3D打印技术,发育性髋关节发育不良,骨盆骨折,髋臼骨折,骨盆肿瘤,髋臼肿瘤、髋关节置换”,英文检索词为“3D printing,developmental dysplasia of the hip,DDH,pelvic fracture,acetabular fracture,pelvic tumor,acetabular tumor,replacement or the hip joint”。共检索到103篇文章,根据纳入与排除标准最终纳入41篇文献进行综述。结果与结论:3D打印的优势在于不需要模具,直接通过数字化技术制造复杂的几何模具或模型。将3D打印技术应用在发育性髋关节发育不良、复杂的骨盆及髋臼骨折与髋关节肿瘤的临床治疗中,显著提高了患者满意度,减少了并发症的发生。因此,将3D打印技术用于髋关节疾病的诊治对髋关节疾病的术前指导、术中操作以及术后康复都具有重要临床意义与广阔的应用前景。
