1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, wh...1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, which produces some difficulties to detect the components and to control the quality. Shuanghuanglian preparations（SHLs）, including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese patent medicine and a combined herbal remedy comprised of three herbs：展开更多
运用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)技术研究炒菟丝子在大鼠胆汁中的原型成分及其代谢产物,并明确其在胆汁中的代谢转化规律.采用Agilent ZORBAX HPLC Eclipse Plus C 18(100 mm×3 mm,1.8μm)色谱柱,以0.1%甲...运用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)技术研究炒菟丝子在大鼠胆汁中的原型成分及其代谢产物,并明确其在胆汁中的代谢转化规律.采用Agilent ZORBAX HPLC Eclipse Plus C 18(100 mm×3 mm,1.8μm)色谱柱,以0.1%甲酸水-乙腈为流动相进行梯度洗脱,流速0.4 mL·min^(-1),进样量3μL,柱温30℃.运用电喷雾离子源(ESI),扫描范围m/z 50~1200,正、负离子模式下采集胆汁样品谱图信息.结合相关文献信息自建库,从含药胆汁中共鉴定出28种化学成分,其中原型成分4种,代谢产物24种,炒菟丝子在胆汁中发生代谢转化类型有甲基化、葡萄糖醛酸化、氧化脱氢等.就炒菟丝子在大鼠胆汁的化学成分进行分析和代谢过程研究,可为后期炒菟丝子的体内代谢过程及其药效物质基础研究提供依据和参考.展开更多
基金Supported by the National S&T Supporting Project in the 11th Five-Year Period(No.2006BAI08B04-01).
文摘1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, which produces some difficulties to detect the components and to control the quality. Shuanghuanglian preparations（SHLs）, including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese patent medicine and a combined herbal remedy comprised of three herbs：
