Objective:To apply and verify the application of intelligent audit rules for urine analysis by Cui et al.Method:A total of 1139 urine samples of hospitalized patients in Tai’an Central Hospital from September 2021 to...Objective:To apply and verify the application of intelligent audit rules for urine analysis by Cui et al.Method:A total of 1139 urine samples of hospitalized patients in Tai’an Central Hospital from September 2021 to November 2021 were randomly selected,and all samples were manually microscopic examined after the detection of the UN9000 urine analysis line.The intelligent audit rules(including the microscopic review rules and manual verification rules)were validated based on the manual microscopic examination and manual audit,and the rules were adjusted to apply to our laboratory.The laboratory turnaround time(TAT)before and after the application of intelligent audit rules was compared.Result:The microscopic review rate of intelligent rules was 25.63%(292/1139),the true positive rate,false positive rate,true negative rate,and false negative rate were 27.66%(315/1139),6.49%(74/1139),62.34%(710/1139)and 3.51%(40/1139),respectively.The approval consistency rate of manual verification rules was 84.92%(727/856),the approval inconsistency rate was 0%(0/856),the interception consistency rate was 12.61%(108/856),and the interception inconsistency rate was 0%(0/856).Conclusion:The intelligence audit rules for urine analysis by Cui et al.have good clinical applicability in our laboratory.展开更多
The total amount of 100 subjects were contributed in this review and all were the students who are studying in Bahauddin Zakariya University Multan,Pakistan.The bilirubin is metabolized in the gut which produced a col...The total amount of 100 subjects were contributed in this review and all were the students who are studying in Bahauddin Zakariya University Multan,Pakistan.The bilirubin is metabolized in the gut which produced a colorless pigment known as urobilinogen.It is by-product of bilirubin which is used to break down the red blood cells in hemolysis.Salty food contains usually more minerals and vitamins while sweet food is enriched with carbohydrates,water and many other fats soluble substances.Every person has a unique taste according to their taste buds.A questionnaire based was made to relate the urobilinogen with the food(salty or sweet).Urinalysis is a method which is used to measure the urobilinogen in urine.It was concluded that there is a scientific relation between the presence of urobilinogen in urine with eating of salty or sugary food.Table no.1 represents that urobilinogen play important role in the choice of eating either salty food more or sweet.展开更多
Background:BK polyomavirus(BKPyV)-associated nephropathy(BKPyVAN)is an important cause of dysfunction and failure of renal transplants.This study aimed to assess the diagnostic performance of morning urine specific gr...Background:BK polyomavirus(BKPyV)-associated nephropathy(BKPyVAN)is an important cause of dysfunction and failure of renal transplants.This study aimed to assess the diagnostic performance of morning urine specific gravity(MUSG)in diagnosing BKPyVAN in kidney transplant recipients.Methods:A total of 87 patients,including 27 with BKPyVAN,22 with isolated BKPyV viruria,18 with T cell-mediated rejection(TCMR),and 20 with stable graft function,were enrolled in the First Affiliated Hospital of Sun Yat-Sen University from March 2015 to February 2017.MUSG at biopsy and during a follow-up period of 24 months after biopsy was collected and analyzed.Receiver operating characteristic(ROC)curve analysis was used to determine the ability of MUSG to discriminate BKPyVAN.Results:At biopsy,the MUSG of BKPyVAN group(1.008±0.003)was significantly lower than that of isolated BK viruria group(1.013±0.004,P<0.001),TCMR group(1.011±0.003,P=0.027),and control group(1.014±0.006,P<0.001).There was no significant difference in MUSG among the isolated BK viruria group,TCMR group,and control group(P=0.253).In BKPyVAN group,the timing and trend of MUSG elevate were consistent with the timing and trend of the decline of viral load in urine and plasma,reaching a statistical difference at 3 months after treatment(1.012±0.003,P<0.001)compared with values at diagnosis.ROC analysis indicated that the optimal cut-off value of MUSG for diagnosis of BKPyVAN was 1.009,with an area under the ROC curve(AUC)of 0.803(95%confidence interval[CI]:0.721–0.937).For differentiating BKPyVAN and TCMR,the optimal MUSG cut-off value was 1.010,with an AUC of 0.811(95%CI:0.687–0.934).Conclusion:Combined detection of MUSG and BKPyV viruria is valuable for predicting BKPyVAN and distinguishing BKPyVAN from TCMR in renal transplant recipients.展开更多
Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher r...Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher rate of voiding within 5 minutes using this method.We evaluated this method in an Irish hospital providing secondary care.Methods A non-blinded,randomized,controlled trial was carried out.Eligible infants were between 1 and 12 months of age,who required urine sampling as part of clinical care.Participants were randomly allocated to receive the intervention(Quick Wee Method—supra-pubic stimulation with cold saline)or the control(usual care—clean catch with no bladder stimulation)for 5 min.Primary outcome was voiding of urine within 5 min.Results A total of 140 infants were included in this study(73 in intervention group;67 in control group).Baseline characteristics were similar.25%in the intervention group passed urine in the 5-min trial period compared with 18%in the control group[P=0.4,absolute diference 7%(95%confdence interval:−7%to+20%)].Conclusion The Quick Wee method is a simple and inexpensive intervention that did not show a statistically signifcant increase in urine samples obtained in pre-continent infants.展开更多
文摘Objective:To apply and verify the application of intelligent audit rules for urine analysis by Cui et al.Method:A total of 1139 urine samples of hospitalized patients in Tai’an Central Hospital from September 2021 to November 2021 were randomly selected,and all samples were manually microscopic examined after the detection of the UN9000 urine analysis line.The intelligent audit rules(including the microscopic review rules and manual verification rules)were validated based on the manual microscopic examination and manual audit,and the rules were adjusted to apply to our laboratory.The laboratory turnaround time(TAT)before and after the application of intelligent audit rules was compared.Result:The microscopic review rate of intelligent rules was 25.63%(292/1139),the true positive rate,false positive rate,true negative rate,and false negative rate were 27.66%(315/1139),6.49%(74/1139),62.34%(710/1139)and 3.51%(40/1139),respectively.The approval consistency rate of manual verification rules was 84.92%(727/856),the approval inconsistency rate was 0%(0/856),the interception consistency rate was 12.61%(108/856),and the interception inconsistency rate was 0%(0/856).Conclusion:The intelligence audit rules for urine analysis by Cui et al.have good clinical applicability in our laboratory.
文摘The total amount of 100 subjects were contributed in this review and all were the students who are studying in Bahauddin Zakariya University Multan,Pakistan.The bilirubin is metabolized in the gut which produced a colorless pigment known as urobilinogen.It is by-product of bilirubin which is used to break down the red blood cells in hemolysis.Salty food contains usually more minerals and vitamins while sweet food is enriched with carbohydrates,water and many other fats soluble substances.Every person has a unique taste according to their taste buds.A questionnaire based was made to relate the urobilinogen with the food(salty or sweet).Urinalysis is a method which is used to measure the urobilinogen in urine.It was concluded that there is a scientific relation between the presence of urobilinogen in urine with eating of salty or sugary food.Table no.1 represents that urobilinogen play important role in the choice of eating either salty food more or sweet.
基金This work was supported by grants from the National Natural Science Foundation of China(No.81770749)the Natural Science Foundation of Guangdong Province(No.2017A030313710)the Basic Scientific Research Fund of Sun Yat-Sen University(No.17ykpy29).
文摘Background:BK polyomavirus(BKPyV)-associated nephropathy(BKPyVAN)is an important cause of dysfunction and failure of renal transplants.This study aimed to assess the diagnostic performance of morning urine specific gravity(MUSG)in diagnosing BKPyVAN in kidney transplant recipients.Methods:A total of 87 patients,including 27 with BKPyVAN,22 with isolated BKPyV viruria,18 with T cell-mediated rejection(TCMR),and 20 with stable graft function,were enrolled in the First Affiliated Hospital of Sun Yat-Sen University from March 2015 to February 2017.MUSG at biopsy and during a follow-up period of 24 months after biopsy was collected and analyzed.Receiver operating characteristic(ROC)curve analysis was used to determine the ability of MUSG to discriminate BKPyVAN.Results:At biopsy,the MUSG of BKPyVAN group(1.008±0.003)was significantly lower than that of isolated BK viruria group(1.013±0.004,P<0.001),TCMR group(1.011±0.003,P=0.027),and control group(1.014±0.006,P<0.001).There was no significant difference in MUSG among the isolated BK viruria group,TCMR group,and control group(P=0.253).In BKPyVAN group,the timing and trend of MUSG elevate were consistent with the timing and trend of the decline of viral load in urine and plasma,reaching a statistical difference at 3 months after treatment(1.012±0.003,P<0.001)compared with values at diagnosis.ROC analysis indicated that the optimal cut-off value of MUSG for diagnosis of BKPyVAN was 1.009,with an area under the ROC curve(AUC)of 0.803(95%confidence interval[CI]:0.721–0.937).For differentiating BKPyVAN and TCMR,the optimal MUSG cut-off value was 1.010,with an AUC of 0.811(95%CI:0.687–0.934).Conclusion:Combined detection of MUSG and BKPyV viruria is valuable for predicting BKPyVAN and distinguishing BKPyVAN from TCMR in renal transplant recipients.
文摘Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher rate of voiding within 5 minutes using this method.We evaluated this method in an Irish hospital providing secondary care.Methods A non-blinded,randomized,controlled trial was carried out.Eligible infants were between 1 and 12 months of age,who required urine sampling as part of clinical care.Participants were randomly allocated to receive the intervention(Quick Wee Method—supra-pubic stimulation with cold saline)or the control(usual care—clean catch with no bladder stimulation)for 5 min.Primary outcome was voiding of urine within 5 min.Results A total of 140 infants were included in this study(73 in intervention group;67 in control group).Baseline characteristics were similar.25%in the intervention group passed urine in the 5-min trial period compared with 18%in the control group[P=0.4,absolute diference 7%(95%confdence interval:−7%to+20%)].Conclusion The Quick Wee method is a simple and inexpensive intervention that did not show a statistically signifcant increase in urine samples obtained in pre-continent infants.
