<p style="text-align:justify;"> <span><span>UV radiation plays an important role not only in plant growth and development </span><span>but also in the accumulation of essential ...<p style="text-align:justify;"> <span><span>UV radiation plays an important role not only in plant growth and development </span><span>but also in the accumulation of essential nutrients and health-promoting</span><span> phytochemicals in plants. The main objective of this study was to examine the effects of supplemental UV-A, UV-B, and UV-AB on the nutritional quality of lettuce (</span><i><span>Lactuca sativa</span></i><span>, cv. red leaf </span></span><span>“</span><span>New Red Fire</span><span>”</span><span> and green leaf </span><span>“</span><span>Two Star</span><span>”</span><span><span>) and tomato (</span><i><span>Solanum lycopersicum</span></i></span><i><span> </span></i><span>L., cv. BHN-589) grown in a greenhouse. Supplemental UV radiation was provided by UV lamps 5</span><span> </span><span>-</span><span> </span><span>6 days prior to harvest. Supplemental UV-A produced higher accumulation of total phenolic compounds and higher antioxidant capacity in red leaf lettuce compared to other treatments. Overall, supplemental UV-A produced a stronger response than other UV treatments and control in the accumulation of many phenolic compounds including luteolin-7-glucoside, quecetin-3-glucoside, and apigenin-3-glucoside in red leaf lettuce. However, UV-B and UV-AB had a negative response in the accumulation of many phenolic compounds including chlorogenic acid, luteolin-7-glucoside, quercetin-3-glucoside, and apigenin-3-glucoside in both red and green leaf lettuce varieties. In tomato fruits, supplemental UV-A had no effect on their total phenolic concentration. However, supplemental UV-B radiation for 3 h or UV-AB radiation for 9 h exposure produced higher total phenolic concentration in the fruits compared to other supplemental UV treatments. Supplemental UV-AB (3 hexposure) was generally more effective than other UV treatments in increasing the accumulation of a number of phenolic compounds including chlorogenic acid, caffeic acid, chicoric acid, luteolin-7-glucoside, and other flavonoids in ripe tomato fruits. Supplemental UV-A produced higher accumulation of carotenoids including lutein and</span><span> </span><span>β</span><span>-carotene than other supplemental UV treatments, while supplemental UV-AB increased the accumulation of lycopene in fully ripe tomatoes. With regard to the essential nutrients, green leaf lettuce was more responsive to the supplemental UV treatments than red leaf lettuce. All the supplemental UV treatments produced an increase in protein concentration in the leaves of green leaf lettuce. However, supplemental UV-AB produced a stronger response compared to the control and other UV treatments in increasing the accumulation of many nutrients including protein, phosphorus, potassium, sulfur, and zinc in green leaf lettuce </span><span>“</span><span>Two Star</span><span>”</span><span>. Supplemental UV-treatments did not affect the accumulation of any essential nutrients in fully ripe tomato fruits. The results show that supplemental UV enhances the nutritional quality of lettuce in relation to both health-promoting phytochemicals and essential nutrients. Similarly, supplemental UV enhances nutritional quality in tomato fruits with higher accumulation of both phenolic compounds and carotenoids than </span><span>does </span><span>the control treatment.</span> </p>展开更多
随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素...随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素对增强马铃薯耐UV-B辐射的分子机制,以马铃薯品种合作88为试验材料,对外源褪黑素处理UV-B辐射下的马铃薯叶片进行转录组测序,并进行基因本体(gene ontology, GO)功能聚类分析及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)功能聚类分析。结果表明,褪黑素可以促进马铃薯植株中缬氨酸、亮氨酸和异亮氨酸生物合成及精氨酸、脯氨酸代谢,提高内质网上蛋白质的加工水平,促进抗氧化物质编码基因的表达,激活UVR8等UV-B辐射的响应基因,从而缓解UV-B辐射对马铃薯植株的危害。综上所述,适量浓度的褪黑素对提高马铃薯耐UV-B辐射有积极作用,研究结果可为马铃薯抗UV-B辐射机制解析提供新的证据,并为其抗UV-B辐射研究提供一定的分子基础。展开更多
Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by appl...Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.展开更多
文摘<p style="text-align:justify;"> <span><span>UV radiation plays an important role not only in plant growth and development </span><span>but also in the accumulation of essential nutrients and health-promoting</span><span> phytochemicals in plants. The main objective of this study was to examine the effects of supplemental UV-A, UV-B, and UV-AB on the nutritional quality of lettuce (</span><i><span>Lactuca sativa</span></i><span>, cv. red leaf </span></span><span>“</span><span>New Red Fire</span><span>”</span><span> and green leaf </span><span>“</span><span>Two Star</span><span>”</span><span><span>) and tomato (</span><i><span>Solanum lycopersicum</span></i></span><i><span> </span></i><span>L., cv. BHN-589) grown in a greenhouse. Supplemental UV radiation was provided by UV lamps 5</span><span> </span><span>-</span><span> </span><span>6 days prior to harvest. Supplemental UV-A produced higher accumulation of total phenolic compounds and higher antioxidant capacity in red leaf lettuce compared to other treatments. Overall, supplemental UV-A produced a stronger response than other UV treatments and control in the accumulation of many phenolic compounds including luteolin-7-glucoside, quecetin-3-glucoside, and apigenin-3-glucoside in red leaf lettuce. However, UV-B and UV-AB had a negative response in the accumulation of many phenolic compounds including chlorogenic acid, luteolin-7-glucoside, quercetin-3-glucoside, and apigenin-3-glucoside in both red and green leaf lettuce varieties. In tomato fruits, supplemental UV-A had no effect on their total phenolic concentration. However, supplemental UV-B radiation for 3 h or UV-AB radiation for 9 h exposure produced higher total phenolic concentration in the fruits compared to other supplemental UV treatments. Supplemental UV-AB (3 hexposure) was generally more effective than other UV treatments in increasing the accumulation of a number of phenolic compounds including chlorogenic acid, caffeic acid, chicoric acid, luteolin-7-glucoside, and other flavonoids in ripe tomato fruits. Supplemental UV-A produced higher accumulation of carotenoids including lutein and</span><span> </span><span>β</span><span>-carotene than other supplemental UV treatments, while supplemental UV-AB increased the accumulation of lycopene in fully ripe tomatoes. With regard to the essential nutrients, green leaf lettuce was more responsive to the supplemental UV treatments than red leaf lettuce. All the supplemental UV treatments produced an increase in protein concentration in the leaves of green leaf lettuce. However, supplemental UV-AB produced a stronger response compared to the control and other UV treatments in increasing the accumulation of many nutrients including protein, phosphorus, potassium, sulfur, and zinc in green leaf lettuce </span><span>“</span><span>Two Star</span><span>”</span><span>. Supplemental UV-treatments did not affect the accumulation of any essential nutrients in fully ripe tomato fruits. The results show that supplemental UV enhances the nutritional quality of lettuce in relation to both health-promoting phytochemicals and essential nutrients. Similarly, supplemental UV enhances nutritional quality in tomato fruits with higher accumulation of both phenolic compounds and carotenoids than </span><span>does </span><span>the control treatment.</span> </p>
文摘随着人类活动对大气层臭氧的破坏,导致到达地面的UV-B辐射增强,而严重的UV-B辐射会直接导致马铃薯产量、品质大幅度降低。马铃薯作为云南省重要的粮食作物,由于云南省具有特殊的地理位置,使马铃薯面临的UV-B辐射较为严峻。为探究褪黑素对增强马铃薯耐UV-B辐射的分子机制,以马铃薯品种合作88为试验材料,对外源褪黑素处理UV-B辐射下的马铃薯叶片进行转录组测序,并进行基因本体(gene ontology, GO)功能聚类分析及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)功能聚类分析。结果表明,褪黑素可以促进马铃薯植株中缬氨酸、亮氨酸和异亮氨酸生物合成及精氨酸、脯氨酸代谢,提高内质网上蛋白质的加工水平,促进抗氧化物质编码基因的表达,激活UVR8等UV-B辐射的响应基因,从而缓解UV-B辐射对马铃薯植株的危害。综上所述,适量浓度的褪黑素对提高马铃薯耐UV-B辐射有积极作用,研究结果可为马铃薯抗UV-B辐射机制解析提供新的证据,并为其抗UV-B辐射研究提供一定的分子基础。
基金supported by grants from the National Science Foundation of China (Grant Nos.32202466,32172593,and32272746)。
文摘Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.
