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利用CRISPR/Cas9技术构建Quaking敲除的小鼠胚胎成纤维细胞株
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作者 高登科 马白荣 +5 位作者 郭怡莹 刘薇 刘田 靳亚平 江舟 陈华涛 《生物技术通报》 CAS CSCD 北大核心 2024年第2期65-72,共8页
【目的】利用CRISPR/Cas9技术构建小鼠胚胎成纤维细胞(NIH3T3)Quaking基因敲除细胞株,并检测Quaking基因对NIH3T3细胞增殖能力的影响。【方法】首先,利用在线网站设计两条靶向作用于Quaking外显子的sgRNA,成功构建了两个分别靶向Quakin... 【目的】利用CRISPR/Cas9技术构建小鼠胚胎成纤维细胞(NIH3T3)Quaking基因敲除细胞株,并检测Quaking基因对NIH3T3细胞增殖能力的影响。【方法】首先,利用在线网站设计两条靶向作用于Quaking外显子的sgRNA,成功构建了两个分别靶向Quaking基因第1、第2外显子的CRISPR/Cas9重组慢病毒质粒。将构建的Quaking基因CRISPR/Cas9重组慢病毒载体和pcDNA3.1-Quaking过表达质粒共转染至HEK293T细胞中,通过Western blot实验检测Quaking蛋白的敲除效率。其次,将筛选得到的敲除效率高的重组慢病毒质粒(LentiCRISPRv2-sgRNA1)与辅助包装质粒共转染入HEK293T细胞进行慢病毒包装,慢病毒转导NIH3T3细胞后,利用嘌呤霉素筛选阳性单克隆细胞株。最后,通过Western blot及免疫荧光染色鉴定敲除效果。【结果】发现Quaking蛋白在该细胞株中不表达,并测序证实了发生片段敲除。CCK8检测发现,Quaking基因敲除显著抑制了NIH3T3细胞的增殖能力。【结论】本研究首次通过CRISPR/Cas9技术成功构建了小鼠胚胎成纤维细胞(NIH3T3)Quaking基因敲除细胞株,为后续研究Quaking基因在小鼠生理功能调节中的作用机制提供了体外模型基础。 展开更多
关键词 quaking CRISPR/Cas9 小鼠胚胎成纤维细胞 基因敲除
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Geochemical characteristics and exploration significance of ultra-deep Sinian oil and gas from Well Tashen 5,Tarim Basin,NW China 被引量:3
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作者 Zicheng Cao Anlai Ma +4 位作者 Qinqi Xu Quanyong Pan Kai Shang Fan Feng Yongli Liu 《Energy Geoscience》 EI 2024年第1期24-36,共13页
The Well Tashen 5(TS5),drilled and completed at a vertical depth of 9017 m in the Tabei Uplift of the Tarim Basin,NW China,is the deepest well in Asia.It has been producing both oil and gas from the Sinian at a depth ... The Well Tashen 5(TS5),drilled and completed at a vertical depth of 9017 m in the Tabei Uplift of the Tarim Basin,NW China,is the deepest well in Asia.It has been producing both oil and gas from the Sinian at a depth of 8780e8840 m,also the deepest in Asia in terms of oil discovery.In this paper,the geochemical characteristics of Sinian oil and gas from the well were investigated and compared with those of Cambrian oil and gas discovered in the same basin.The oil samples,with Pr/Ph ratio of 0.78 and a whole oil carbon isotopic value of31.6‰,have geochemical characteristics similar to those of Ordovician oils from the No.1 fault in the North Shuntuoguole area(also named Shunbei area)and the Middle Cambrian oil from wells Zhongshen 1(ZS1)and Zhongshen 5(ZS5)of Tazhong Uplift.The maturity of light hydrocarbons,diamondoids and aromatic fractions all suggest an approximate maturity of 1.5%e1.7%Ro for the samples.The(4-+3-)methyldiamantane concentration of the samples is 113.5 mg/g,indicating intense cracking with a cracking degree of about 80%,which is consistent with the high bottom hole temperature(179℃).The Sinian gas samples are dry with a dryness coefficient of 0.97.The gas is a mixture of kerogen-cracking gas and oil-cracking gas and has Ro values ranging between 1.5%and 1.7%,and methane carbon isotopic values of41.6‰.Based on the equivalent vitrinite reflectance(R_(eqv)=1.51%e1.61%)and the thermal evolution of source rocks from the Cambrian Yu'ertusi Formation of the same well,it is proposed that the Sinian oil and gas be mainly sourced from the Cambrian Yu'ertusi Formation during the Himalayan period but probably also be joined by hydrocarbon of higher maturity that migrated from other source rocks in deeper formations.The discovery of Sinian oil and gas from Well TS5 suggests that the ancient ultra-deep strata in the northern Tarim Basin have the potential for finding volatile oil or condensate reservoirs. 展开更多
关键词 ultra-deep Sinian oil and gas Oil-cracking Well Tashen 5 Tarim Basin
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Possible Connections between Seasons and Ultra-Deep Earthquakes Worldwide 被引量:2
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作者 Marilia Hagen A. Azevedo 《Natural Science》 2018年第7期288-302,共15页
The aim of this investigation is to find possible changes in ultra-deep earthquakes (UDQ) during different seasons of the year. In the acquisition of data for our previous work we observed an inexplicable pattern of g... The aim of this investigation is to find possible changes in ultra-deep earthquakes (UDQ) during different seasons of the year. In the acquisition of data for our previous work we observed an inexplicable pattern of growth of ultra-deep tremors (UDQ) during the studied period. Apparently, there is no viable explanation for growth occurring at such a level, presumably in the asthenosphere. Current research and theories developed for the inner layers of the Earth do not explain such variations. Therefore, a possible explanation would be in external factors such as the seasons of the year, which are determined by changes in the Earth’s axial tilt, and therefore the portion of the earth that is angled toward the sun. This paper focuses exclusively on UDQ events. To simplify the calculations, we consider four main locations;this includes one more region than our previous paper but includes only UDQ data. The results showed that during spring and autumn UDQ events grew slightly in both Northern and Southern Hemispheres. It is also suggested that a contributor to UDQ events is friction from the subducting lithosphere against the continental plates. 展开更多
关键词 Sun MOON Earth ultra-deep quakes SEASONS
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A review of reservoir damage during hydraulic fracturing of deep and ultra-deep reservoirs 被引量:2
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作者 Kun Zhang Xiong-Fei Liu +6 位作者 Dao-Bing Wang Bo Zheng Tun-Hao Chen Qing Wang Hao Bai Er-Dong Yao Fu-Jian Zhou 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期384-409,共26页
Deep and ultra-deep reservoirs have gradually become the primary focus of hydrocarbon exploration as a result of a series of significant discoveries in deep hydrocarbon exploration worldwide.These reservoirs present u... Deep and ultra-deep reservoirs have gradually become the primary focus of hydrocarbon exploration as a result of a series of significant discoveries in deep hydrocarbon exploration worldwide.These reservoirs present unique challenges due to their deep burial depth(4500-8882 m),low matrix permeability,complex crustal stress conditions,high temperature and pressure(HTHP,150-200℃,105-155 MPa),coupled with high salinity of formation water.Consequently,the costs associated with their exploitation and development are exceptionally high.In deep and ultra-deep reservoirs,hydraulic fracturing is commonly used to achieve high and stable production.During hydraulic fracturing,a substantial volume of fluid is injected into the reservoir.However,statistical analysis reveals that the flowback rate is typically less than 30%,leaving the majority of the fluid trapped within the reservoir.Therefore,hydraulic fracturing in deep reservoirs not only enhances the reservoir permeability by creating artificial fractures but also damages reservoirs due to the fracturing fluids involved.The challenging“three-high”environment of a deep reservoir,characterized by high temperature,high pressure,and high salinity,exacerbates conventional forms of damage,including water sensitivity,retention of fracturing fluids,rock creep,and proppant breakage.In addition,specific damage mechanisms come into play,such as fracturing fluid decomposition at elevated temperatures and proppant diagenetic reactions at HTHP conditions.Presently,the foremost concern in deep oil and gas development lies in effectively assessing the damage inflicted on these reservoirs by hydraulic fracturing,comprehending the underlying mechanisms,and selecting appropriate solutions.It's noteworthy that the majority of existing studies on reservoir damage primarily focus on conventional reservoirs,with limited attention given to deep reservoirs and a lack of systematic summaries.In light of this,our approach entails initially summarizing the current knowledge pertaining to the types of fracturing fluids employed in deep and ultra-deep reservoirs.Subsequently,we delve into a systematic examination of the damage processes and mechanisms caused by fracturing fluids within the context of hydraulic fracturing in deep reservoirs,taking into account the unique reservoir characteristics of high temperature,high pressure,and high in-situ stress.In addition,we provide an overview of research progress related to high-temperature deep reservoir fracturing fluid and the damage of aqueous fracturing fluids to rock matrix,both artificial and natural fractures,and sand-packed fractures.We conclude by offering a summary of current research advancements and future directions,which hold significant potential for facilitating the efficient development of deep oil and gas reservoirs while effectively mitigating reservoir damage. 展开更多
关键词 Artificial fracture Deep and ultra-deep reservoir Fracture conductivity Fracturing fluid Hydraulic fracturing Reservoir damage
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Deep and Ultra-Deep Earthquakes Worldwide, Possible Anomalies in South America 被引量:3
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作者 M. Hagen A. Azevedo 《Natural Science》 2018年第6期199-213,共15页
The aim of this paper is to evaluate the worldwide variation of deep and ultra-deep earthquakes (DQ and UDQ) during the period 1996-2017. This project found only three locations around the globe presenting this kind o... The aim of this paper is to evaluate the worldwide variation of deep and ultra-deep earthquakes (DQ and UDQ) during the period 1996-2017. This project found only three locations around the globe presenting this kind of seismicity. Although there are other global settings showing deep seismicity, they are not periodical and cannot be considered by a statistical view. The three areas with intense activity for DQ and UDQ events are located mostly in subduction areas. The largest variations of DQ and UDQ border the Pacific Ocean and include the North Pacific, South Pacific, and South America. The major difference in this set is that the first two sites are subduction zones and the South American occurrences happened in the interior of the continent. Another anomaly is an internal layer between 300 - 500 km in South America that shows no tremors in the period studied. However, below 500 km activity reappears, even at extreme depths of up to 650 km. We suggested that the reason for those occurrences would be due to an anomaly in the asthenosphere in this region. This anomaly would probably be presenting a breakable material that was pushed by the Nazca platform against the South America plate. Other depths below 100 km in all the regions are discussed as well. We suggested that the reason for those occurrences was an anomaly created in the asthenosphere as part of the process of the South America collision with the Nazca plate. Part of the Nazca plate has subducted below South America, creating a slab as deep as 500 km. The convergent slab is still moving against South America and sinking due to the gravity and rotation of the Earth. The discrepancies in the occurrences we tracked at different locations indicated that this slab had different thicknesses around South America. We found similar results for Vanuatu and Fiji;in these regions UDQ events occur at the subduction zones under the ocean with depths greater than 700 km. Here, a possible explanation is that part of the lithosphere is subducted at these depths and is causing tremors. 展开更多
关键词 DEEP EARTHquakes (DQ) ultra-deep EARTHquakes (UDQ) South AMERICA
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Multi-lump Kinetic Parameter Estimation and Simulation of Trickle-bed Reactor for Ultra-deep Hydrodesulfurization of Diesel
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作者 Huang Zhen Zhang Xun +4 位作者 Qiao Aijun Xue Nan Liu Kaixiang Xu Song Wang He 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS CSCD 2024年第3期147-157,共11页
A three-lumping Langmuir-Hinshelwood kinetic model was established based on the structures and reactivities of sulfur compounds.This model described the ultra-deep hydrodesulfurization(UDHDS)performance of diesel,redu... A three-lumping Langmuir-Hinshelwood kinetic model was established based on the structures and reactivities of sulfur compounds.This model described the ultra-deep hydrodesulfurization(UDHDS)performance of diesel,reducing sulfur content from 10000μg/g to less than 10μg/g,with experimental and predicted data showing a discrepancy of less than 10%.The diesel UDHDS reaction was simulated by combining the mass transfer,reaction kinetics model,and physical properties of diesel.The results showed how the concentrations of H2S,hydrogen,and sulfur in the gas,liquid,and solid phases varied along the reactor length.Moreover,the study discussed the effects of each process parameter and impurity concentrations(H2S,basic nitrogen and,non-basic nitrogen)on diesel UDHDS. 展开更多
关键词 DIESEL TBR ultra-deep HDS kinetic model simulation
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Supramolecular polymer-based gel fracturing fluid with a double network applied in ultra-deep hydraulic fracturing
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作者 Yong-Ping Huang Yong Hu +5 位作者 Chang-Long Liu Yi-Ning Wu Chen-Wei Zou Li-Yuan Zhang Ming-Wei Zhao Cai-Li Dai 《Petroleum Science》 SCIE EI CAS CSCD 2024年第3期1875-1888,共14页
A gel based on polyacrylamide,exhibiting delayed crosslinking characteristics,emerges as the preferred solution for mitigating degradation under conditions of high temperature and extended shear in ultralong wellbores... A gel based on polyacrylamide,exhibiting delayed crosslinking characteristics,emerges as the preferred solution for mitigating degradation under conditions of high temperature and extended shear in ultralong wellbores.High viscosity/viscoelasticity of the fracturing fluid was required to maintain excellent proppant suspension properties before gelling.Taking into account both the cost and the potential damage to reservoirs,polymers with lower concentrations and molecular weights are generally preferred.In this work,the supramolecular action was integrated into the polymer,resulting in significant increases in the viscosity and viscoelasticity of the synthesized supramolecular polymer system.The double network gel,which is formed by the combination of the supramolecular polymer system and a small quantity of Zr-crosslinker,effectively resists temperature while minimizing permeability damage to the reservoir.The results indicate that the supramolecular polymer system with a molecular weight of(268—380)×10^(4)g/mol can achieve the same viscosity and viscoelasticity at 0.4 wt%due to the supramolecular interaction between polymers,compared to the 0.6 wt%traditional polymer(hydrolyzed polyacrylamide,molecular weight of 1078×10^(4)g/mol).The supramolecular polymer system possessed excellent proppant suspension properties with a 0.55 cm/min sedimentation rate at 0.4 wt%,whereas the0.6 wt%traditional polymer had a rate of 0.57 cm/min.In comparison to the traditional gel with a Zrcrosslinker concentration of 0.6 wt%and an elastic modulus of 7.77 Pa,the double network gel with a higher elastic modulus(9.00 Pa)could be formed only at 0.1 wt%Zr-crosslinker,which greatly reduced the amount of residue of the fluid after gel-breaking.The viscosity of the double network gel was66 m Pa s after 2 h shearing,whereas the traditional gel only reached 27 m Pa s. 展开更多
关键词 ultra-deep reservoir Gel fracturing fluid Double network Supramolecular polymer system Proppant suspension property
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Research advances on the mechanisms of reservoir formation and hydrocarbon accumulation and the oil and gas development methods of deep and ultra-deep marine carbonates
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作者 MA Yongsheng CAI Xunyu +9 位作者 LI Maowen LI Huili ZHU Dongya QIU Nansheng PANG Xiongqi ZENG Daqian KANG Zhijiang MA Anlai SHI Kaibo ZHANG Juntao 《Petroleum Exploration and Development》 SCIE 2024年第4期795-812,共18页
Based on the new data of drilling, seismic, logging, test and experiments, the key scientific problems in reservoir formation, hydrocarbon accumulation and efficient oil and gas development methods of deep and ultra-d... Based on the new data of drilling, seismic, logging, test and experiments, the key scientific problems in reservoir formation, hydrocarbon accumulation and efficient oil and gas development methods of deep and ultra-deep marine carbonate strata in the central and western superimposed basin in China have been continuously studied.(1) The fault-controlled carbonate reservoir and the ancient dolomite reservoir are two important types of reservoirs in the deep and ultra-deep marine carbonates. According to the formation origin, the large-scale fault-controlled reservoir can be further divided into three types:fracture-cavity reservoir formed by tectonic rupture, fault and fluid-controlled reservoir, and shoal and mound reservoir modified by fault and fluid. The Sinian microbial dolomites are developed in the aragonite-dolomite sea. The predominant mound-shoal facies, early dolomitization and dissolution, acidic fluid environment, anhydrite capping and overpressure are the key factors for the formation and preservation of high-quality dolomite reservoirs.(2) The organic-rich shale of the marine carbonate strata in the superimposed basins of central and western China are mainly developed in the sedimentary environments of deep-water shelf of passive continental margin and carbonate ramp. The tectonic-thermal system is the important factor controlling the hydrocarbon phase in deep and ultra-deep reservoirs, and the reformed dynamic field controls oil and gas accumulation and distribution in deep and ultra-deep marine carbonates.(3) During the development of high-sulfur gas fields such as Puguang, sulfur precipitation blocks the wellbore. The application of sulfur solvent combined with coiled tubing has a significant effect on removing sulfur blockage. The integrated technology of dual-medium modeling and numerical simulation based on sedimentary simulation can accurately characterize the spatial distribution and changes of the water invasion front.Afterward, water control strategies for the entire life cycle of gas wells are proposed, including flow rate management, water drainage and plugging.(4) In the development of ultra-deep fault-controlled fractured-cavity reservoirs, well production declines rapidly due to the permeability reduction, which is a consequence of reservoir stress-sensitivity. The rapid phase change in condensate gas reservoir and pressure decline significantly affect the recovery of condensate oil. Innovative development methods such as gravity drive through water and natural gas injection, and natural gas drive through top injection and bottom production for ultra-deep fault-controlled condensate gas reservoirs are proposed. By adopting the hierarchical geological modeling and the fluid-solid-thermal coupled numerical simulation, the accuracy of producing performance prediction in oil and gas reservoirs has been effectively improved. 展开更多
关键词 deep and ultra-deep marine carbonate mechanisms of hydrocarbon accumulation reef-beach facies high-sulfur sour gas reservoirs ultra-deep fault-controlled fractured-cavity reservoir wellbore sulfur deposition fluid-solid-thermal numerical simulation
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A Novel Fracturing Fluid with High-Temperature Resistance for Ultra-Deep Reservoirs
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作者 Lian Liu Liang Li +2 位作者 Kebo Jiao Junwei Fang Yun Luo 《Fluid Dynamics & Materials Processing》 EI 2024年第5期975-987,共13页
Ultra-deep reservoirs play an important role at present in fossil energy exploitation.Due to the related high temperature,high pressure,and high formation fracture pressure,however,methods for oil well stimulation do ... Ultra-deep reservoirs play an important role at present in fossil energy exploitation.Due to the related high temperature,high pressure,and high formation fracture pressure,however,methods for oil well stimulation do not produce satisfactory results when conventional fracturing fluids with a low pumping rate are used.In response to the above problem,a fracturing fluid with a density of 1.2~1.4 g/cm^(3)was developed by using Potassium formatted,hydroxypropyl guanidine gum and zirconium crosslinking agents.The fracturing fluid was tested and its ability to maintain a viscosity of 100 mPa.s over more than 60 min was verified under a shear rate of 1701/s and at a temperature of 175℃.This fluid has good sand-carrying performances,a low viscosity after breaking the rubber,and the residue content is less than 200 mg/L.Compared with ordinary reconstruction fluid,it can increase the density by 30%~40%and reduce the wellhead pressure of 8000 m level reconstruction wells.Moreover,the new fracturing fluid can significantly mitigate safety risks. 展开更多
关键词 ultra-deep reservoir high-temperature resistance weighted fracturing fluid guanidine gum potassium formatted
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Control of Earth system evolution on the formation and enrichment of marine ultra-deep petroleum in China
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作者 ZHANG Shuichang WANG Huajian +3 位作者 SU Jin WANG Xiaomei HE Kun LIU Yuke 《Petroleum Exploration and Development》 SCIE 2024年第4期870-885,共16页
Taking the Paleozoic of the Sichuan and Tarim basins in China as example,the controlling effects of the Earth system evolution and multi-spherical interactions on the formation and enrichment of marine ultra-deep petr... Taking the Paleozoic of the Sichuan and Tarim basins in China as example,the controlling effects of the Earth system evolution and multi-spherical interactions on the formation and enrichment of marine ultra-deep petroleum in China have been elaborated.By discussing the development of“source-reservoir-seal”controlled by the breakup and assembly of supercontinents and regional tectonic movements,and the mechanisms of petroleum generation and accumulation controlled by temperature-pressure system and fault conduit system,Both the South China and Tarim blocks passed through the intertropical convergence zone(ITCZ)of the low-latitude Hadley Cell twice during their drifts,and formed hydrocarbon source rocks with high quality.It is proposed that deep tectonic activities and surface climate evolution jointly controlled the types and stratigraphic positions of ultra-deep hydrocarbon source rocks,reservoirs,and seals in the Sichuan and Tarim basins,forming multiple petroleum systems in the Ediacaran-Cambrian,Cambrian-Ordovician,Cambrian-Permian and Permian-Triassic strata.The matching degree of source-reservoir-seal,the type of organic matter in source rocks,the deep thermal regime of basin,and the burial-uplift process across tectonic periods collectively control the entire process from the generation to the accumulation of oil and gas.Three types of oil and gas enrichment models are formed,including near-source accumulation in platform marginal zones,distant-source accumulation in high-energy beaches through faults,and three-dimensional accumulation in strike-slip fault zones,which ultimately result in the multi-layered natural gas enrichment in ultra-deep layers of the Sichuan Basin and co-enrichment of oil and gas in the ultra-deep layers of the Tarim Basin. 展开更多
关键词 evolution of Earth system multi-spherical interaction Paleozoic marine ultra-deep petroleum Tarim Basin Sichuan Basin
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Research progress and development of deep and ultra-deep drilling fluid technology
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作者 SUN Jinsheng YANG Jingbin +2 位作者 BAI Yingrui LYU Kaihe LIU Fengbao 《Petroleum Exploration and Development》 SCIE 2024年第4期1022-1034,共13页
The research progress of deep and ultra-deep drilling fluid technology systematically reviewed,the key problems existing are analyzed,and the future development direction is proposed.In view of the high temperature,hi... The research progress of deep and ultra-deep drilling fluid technology systematically reviewed,the key problems existing are analyzed,and the future development direction is proposed.In view of the high temperature,high pressure and high stress,fracture development,wellbore instability,drilling fluid lost circulation and other problems faced in the process of deep and ultra-deep complex oil and gas drilling,scholars have developed deep and ultra-deep high-temperature and high-salt resistant water-based drilling fluid technology,high-temperature resistant oil-based/synthetic drilling fluid technology,drilling fluid technology for reservoir protection and drilling fluid lost circulation control technology.However,there are still some key problems such as insufficient resistance to high temperature,high pressure and high stress,wellbore instability and serious lost circulation.Therefore,the development direction of deep and ultra-deep drilling fluid technology in the future is proposed:(1)The technology of high-temperature and high-salt resistant water-based drilling fluid should focus on improving high temperature stability,improving rheological properties,strengthening filtration control and improving compatibility with formation.(2)The technology of oil-based/synthetic drilling fluid resistant to high temperature should further study in the aspects of easily degradable environmental protection additives with low toxicity such as high temperature stabilizer,rheological regulator and related supporting technologies.(3)The drilling fluid technology for reservoir protection should be devoted to the development of new high-performance additives and materials,and further improve the real-time monitoring technology by introducing advanced sensor networks and artificial intelligence algorithms.(4)The lost circulation control of drilling fluid should pay more attention to the integration and application of intelligent technology,the research and application of high-performance plugging materials,the exploration of diversified plugging techniques and methods,and the improvement of environmental protection and production safety awareness. 展开更多
关键词 deep and ultra-deep drilling high temperature resistant drilling fluid reservoir protection drilling fluid lost circulation control safety and environmental protection technical prospects
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Seasonality and Tectonic Influences on Subduction Zones for Ultra-Deep Earthquakes
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作者 Marilia Hagen Anibal Azevedo 《Open Journal of Earthquake Research》 2018年第4期269-284,共16页
Our previous research has found that deep or very deep earthquakes can be influenced by different seasons of the year. It also indicates that other factors may impact the seasonality in addition to these external para... Our previous research has found that deep or very deep earthquakes can be influenced by different seasons of the year. It also indicates that other factors may impact the seasonality in addition to these external parameters. This would explain why the response from Northern Hemisphere and Southern Hemisphere for the seasons is different. In the current research, we will focus on very deep earthquakes over a very long period, 1950-2017, which have high magnitude of M ≥ 6 with depth ≥ 500 km and named ultra-deep earthquakes (UDQ). We will separate such events by coordinates of each subduction area located in the Pacific Ring of Fire to find which effects the seasons have on these specific areas. Former tomographic studies in such regions pointed out that each area mentioned had systematic differences in the slab configuration along arcs. Our conclusions showed that those discrepancies may influence the enhancement of earthquakes in some seasons or months. 展开更多
关键词 ultra-deep EARTHquakes (UDQ) SUBDUCTION Zones TECTONICS
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利用Virtual Quake地震模拟器研究鲜水河断裂带地震活动性与地震复发周期
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作者 李晓帆 马延路 +2 位作者 解滔 岳冲 吉平 《中国地震》 北大核心 2023年第3期472-492,共21页
2022年9月5日四川泸定6.8级地震发生在鲜水河断裂带磨西断裂上。本文依据鲜水河断裂带的断层几何形态、断层面物理力学参数、断层滑移速率等基础资料,构建断裂带三维模型,基于Virtual Quake地震模拟器开展鲜水河断裂带准静态数值模拟。... 2022年9月5日四川泸定6.8级地震发生在鲜水河断裂带磨西断裂上。本文依据鲜水河断裂带的断层几何形态、断层面物理力学参数、断层滑移速率等基础资料,构建断裂带三维模型,基于Virtual Quake地震模拟器开展鲜水河断裂带准静态数值模拟。对七条分支断裂独立模拟的结果显示模拟结果与历史地震的最大震级和复发周期具有较好的一致性。结果表明模型长度影响震级大小,滑移速率影响地震复发周期。断层面几何弯曲展布使得断层面应力分布不均匀,在弯曲处出现应力积累的现象,易触发地震,并出现了地震分段破裂现象,降低了几何弯曲断层的地震频次,为强震的孕育创造了条件。联合模拟的10000年时间内,鲜水河断裂带共发生了33次M≥7.0地震,最大震级为M7.6,地震活动性呈现一定的活跃期与平静期。断层系统地震间的相互应力作用和复杂的断层几何结构影响着地震迁移的复杂性。道孚断裂上相距40年发生两次M≥7.0地震,乾宁断裂北端相距5.8年发生M≥7.0双震,此类现象与历史地震有一定的相似性。 展开更多
关键词 鲜水河断裂带 泸定M_(S)6.8地震 三维数值模拟 地震活动性 VIRTUAL quakE
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Prediction Model of Drilling Costs for Ultra-Deep Wells Based on GA-BP Neural Network 被引量:1
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作者 Wenhua Xu Yuming Zhu +4 位作者 YingrongWei Ya Su YanXu Hui Ji Dehua Liu 《Energy Engineering》 EI 2023年第7期1701-1715,共15页
Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In o... Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In order to improve the prediction accuracy of ultra-deep well drilling costs,the item and the dominant factors of drilling costs in Tarim oilfield are analyzed.Then,those factors of drilling costs are separated into categorical variables and numerous variables.Finally,a BP neural networkmodel with drilling costs as the output is established,and hyper-parameters(initial weights and bias)of the BP neural network is optimized by genetic algorithm(GA).Through training and validation of themodel,a reliable prediction model of ultra-deep well drilling costs is achieved.The average relative error between prediction and actual values is 3.26%.Compared with other models,the root mean square error is reduced by 25.38%.The prediction results of the proposed model are reliable,and the model is efficient,which can provide supporting for the drilling costs control and budget planning of ultra-deep wells. 展开更多
关键词 ultra-deep well drilling costs cost estimation BP neural network genetic algorithm
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The evolution characteristics of positive and negative earthquakes before strong quakes in Kanto area and the Hyugo earthquake in Japan
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作者 王裕仓 宋治平 +2 位作者 细野耕司 陈学中 尹祥础 《Acta Seismologica Sinica(English Edition)》 CSCD 1998年第1期33-40,共8页
This paper studies the evolution charateristics of positive and negative quakes before Hyugo earthquake of M =7.2 and several strong quakes in Kanto area in Japan.The results show that the earthquakes over a certai... This paper studies the evolution charateristics of positive and negative quakes before Hyugo earthquake of M =7.2 and several strong quakes in Kanto area in Japan.The results show that the earthquakes over a certain megnitude are mainly positive ones a certain time before the main shock in or near the focal regions of most strong quakes,and form a concentratingintervals of positive quakes,The main quakes generally locate in or near the areas of positive quake distribution.Negative quakes often occur several months before the main shocks (not excluding positive ones), with the decrease of LURR (Loading/Unloading Response Ratio) values.It possibly shows that earthquake generating process has come to a short term stage.These characteristics may help to predict the time and location of the future earthquakes,and have been applied to the preliminary prediction of the time and the location of the earthquake of M =6.6 on Sep.11,1996 in Kanto area. 展开更多
关键词 Load/Unload Response Ratio (LURR) positive and negative quake earthquake prediction Hyugo earthquake
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RNA结合蛋白quaking:单核-巨噬细胞分化及功能的重要调控者
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作者 武梦琪 邓乐乐 +2 位作者 安珮瑜 汪莉 翟东昇 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2023年第11期1032-1038,共7页
巨噬细胞是可塑性较强的一类固有免疫细胞,能够极化为不同表型,发挥吞噬、趋化等多种功能,参与疾病的发生发展。RNA结合蛋白quaking(QKI)通过影响RNA剪切、转位及表达等过程调控单核细胞分化、巨噬细胞极化及多种细胞功能。QKI调控单核... 巨噬细胞是可塑性较强的一类固有免疫细胞,能够极化为不同表型,发挥吞噬、趋化等多种功能,参与疾病的发生发展。RNA结合蛋白quaking(QKI)通过影响RNA剪切、转位及表达等过程调控单核细胞分化、巨噬细胞极化及多种细胞功能。QKI调控单核细胞向巨噬细胞的分化,QKI缺失促进巨噬细胞极化为M1型,发挥促炎表型。相反,QKI过表达则促进其极化为M2型。除此以外,QKI还影响巨噬细胞吞噬受体、趋化因子表达。由于组织定居巨噬细胞特性存在差异,QKI调控巨噬细胞参与多种疾病发展(如动脉粥样硬化、炎症性肠病等)的调控机制多样。主要涉及cAMP反应元件结合蛋白(CREB)转录因子调控、信号转导子与转录激活子1/核因子κB(STAT1/NF-κB)炎症信号通路及吞噬受体mRNA前体剪接等。 展开更多
关键词 quaking(QKI) 单核细胞 巨噬细胞 综述
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Satellites Provided Services for Earthquakes in Nepal and Tibet
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作者 ZONG He 《Aerospace China》 2015年第2期26-29,共4页
An 8.1-magnitude earthquake occurred in Nepal April 25 caused severe injuries,causalities and significant property damage.The quake affected 300,000 people of 19 counties in Tibet Autonomous Region to varying degrees ... An 8.1-magnitude earthquake occurred in Nepal April 25 caused severe injuries,causalities and significant property damage.The quake affected 300,000 people of 19 counties in Tibet Autonomous Region to varying degrees and induced debris flows and landslides in many places.CASC subsidiaries China Centre for Resources Satellite Data and Application(CRESDA)and China Satellite Communications Co.,Ltd(China Satcom)activated respectively the emergency response mechanism quickly to provide information and communications support for the disaster rescue operation. 展开更多
关键词 Tibet disaster DEBRIS counties quickly MAGNITUDE quakE INJURIES emergency METEOROLOGICAL
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Research progress and key issues of ultra-deep oil and gas exploration in China
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作者 HE Dengfa JIA Chengzao +8 位作者 ZHAO Wenzhi XU Fengyin LUO Xiaorong LIU Wenhui TANG Yong GAO Shanlin ZHENG Xiujuan LI Di ZHENG Na 《Petroleum Exploration and Development》 SCIE 2023年第6期1333-1344,共12页
Based on the recent oil and gas discoveries and geological understandings on the ultra-deep strata of sedimentary basins, the formation and occurrence of hydrocarbons in the ultra-deep strata were investigated with re... Based on the recent oil and gas discoveries and geological understandings on the ultra-deep strata of sedimentary basins, the formation and occurrence of hydrocarbons in the ultra-deep strata were investigated with respect to the processes of basin formation, hydrocarbon generation, reservoir formation and hydrocarbon accumulation, and key issues in ultra-deep oil and gas exploration were discussed. The ultra-deep strata in China underwent two extensional-convergent cycles in the Meso-Neoproterozoic Era and the Early Paleozoic Era respectively, with the tectonic-sedimentary differentiation producing the spatially adjacent source-reservoir assemblages. There are diverse large-scale carbonate reservoirs such as mound-beach, dolomite, karst fracture-vug, fractured karst and faulted zone, as well as over-pressured clastic rock and fractured bedrock reservoirs. Hydrocarbons were accumulated in multiple stages, accompanied by adjusting and finalizing in the late stage. The distribution of hydrocarbons is controlled by high-energy beach zone, regional unconformity, paleo-high and large-scale fault zone. The ultra-deep strata endow oil and gas resources as 33% of the remaining total resources, suggesting an important successive domain for hydrocarbon development in China. The large-scale pool-forming geologic units and giant hydrocarbon enrichment zones in ultra-deep strata are key and promising prospects for delivering successive discoveries. The geological conditions and enrichment zone prediction of ultra-deep oil and gas are key issues of petroleum geology. 展开更多
关键词 China ultra-deep oil and gas multi-cycle superimposed basin exploration progress exploration technology light oil natural gas
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全球火山活动时空分布特征及其对强震活动趋势的指示 被引量:1
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作者 石富强 王芃 +5 位作者 杨晨艺 王光明 刘洁 邵志刚 王庆林 贾若 《地震学报》 CSCD 北大核心 2024年第2期273-291,共19页
基于史密森学会火山目录分析了全球火山活动的时空特征,并结合中国地震台网目录讨论了火山活动对全球和中国大陆强震活动趋势的指示意义。结果显示:①全球火山活动表现出较为显著的百年周期特征,且百年周期内火山活动和M≥8.0大震之间... 基于史密森学会火山目录分析了全球火山活动的时空特征,并结合中国地震台网目录讨论了火山活动对全球和中国大陆强震活动趋势的指示意义。结果显示:①全球火山活动表现出较为显著的百年周期特征,且百年周期内火山活动和M≥8.0大震之间存在着频次准同步和能量互补现象;②中国大陆1955年前后强震活动状态的变化可能与同期全球火山活动状态变化密切相关,且二者可能受控于百年周期内地球内部能量积累与释放的状态变化;③2022年汤加火山的剧烈喷发意味着地球内部能量仍在持续释放。结合全球M8地震和中国大陆M7浅源地震的活动特征,认为当前及未来一段时间全球及中国大陆的大震活动状态可能与二十世纪上半叶相似。 展开更多
关键词 火山 地震 时空分布特征 地震趋势 全球和中国大陆
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品牌战略和产品定位决定市场竞争力 本刊对话深圳QUAKES(快可视)中国运营中心总经理曾裕
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作者 三木 本刊资料图 《音响改装技术》 2014年第11期64-65,共2页
任何行业都有其自身的产生、发展和衰落的生命周期,我们把行业的生命周期分为初创期、成长期、稳定期、衰退期四个阶段。具体到行车记录仪行业,目前记录仪行业刚刚从初创期到发展期的节点转变,市场的增长空间相当巨大。从09年至13年初,... 任何行业都有其自身的产生、发展和衰落的生命周期,我们把行业的生命周期分为初创期、成长期、稳定期、衰退期四个阶段。具体到行车记录仪行业,目前记录仪行业刚刚从初创期到发展期的节点转变,市场的增长空间相当巨大。从09年至13年初,可以理解是初创期。去年底至今的行业发展周期节点爆发。这是因为行车记录仪的功能属性,决定着有着庞大的用户基数。在这个潜在基数上,内因有行业经过几年的市场培育与沉淀,及产品功能的价值教育。外因有来自政府,权威媒体乃至用户的消费引导,再加上各销售环节的集体发力,市场井喷是必然的。 展开更多
关键词 行车记录仪 功能属性 用户基数 产品定位 quakes 发展周期 价值教育 稳定期 运营中心 生命周期
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