AIM: To investigate the effect of Jianweiyuyang (JWYY)granule on gastric ulcer recurrence and its mechanism in the treatment of gastric ulcer in rats.METHODS: Gastric ulcer in rats was induced according to Okeba's...AIM: To investigate the effect of Jianweiyuyang (JWYY)granule on gastric ulcer recurrence and its mechanism in the treatment of gastric ulcer in rats.METHODS: Gastric ulcer in rats was induced according to Okeba's method with minor modification and the recurrence model was induced by IL-1β. The expression of vascular endothelial growth factor mRNA (VEGF mRNA) was examined by reverse transcription polymerase chain reaction in gastric ulcer and microvessel density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry.RESULTS: MVD was higher in the JWYY treatment group (14.0±2.62) compared with the normal, model and ranitidine treatment groups (2.2±0.84, 8.8±0.97, 10.4±0.97) in rats (P＜0.01). The expression level of VEGF mRNA in gastric tissues during the healing process of JWYY treatment group rats significantly increased compared with other groups (normal group: 0.190±0.019, model group: 0.642±0.034,ranitidine group: 0.790±0.037, P＜0.01).CONCLUSION: JWYY granules can stimulate angiogenesis and enhance the expression of VEGF mRNA in gastric ulcer rats. This might be the mechanism for JWYY accelerating the ulcer healing, and preventing the recurrence of gastric ulcer.展开更多
Object: To localize the mRNA coding for VEGF atUltrastractural level in human breast cancer by usingdigoxigenin-labeled cDNA probes. Methods: Nonradio-active in situ hybridization at electron microscopic level was emp...Object: To localize the mRNA coding for VEGF atUltrastractural level in human breast cancer by usingdigoxigenin-labeled cDNA probes. Methods: Nonradio-active in situ hybridization at electron microscopic level was employed to detected VEGF mRNA in breast cancer.Result: Cancer cells and endothelial cell of angiogensisshow dark color in experiment sections. No dark color can be found in control sections. Positive hybridization signals showed dark dot and were located in various compartments of the breast cancer cell and endothelial cell in experiment section. No labeling was observed in control sections. In experiment sections, the staining appeared concentrated in cytoplasm and nucleus of the breast cancer cell andendothelial cell. Conclusion: Nonradioactive in situhybridization at electron microscopic level is efficient fordirect observation of the target site mRNA of VEGF in the cytoplasm and nucleus.展开更多
目的:观察甲氨喋吟(MTX)联合中药对胶原诱导关节炎大鼠治疗作用及对血清血管内皮细胞生长因子(VEGF)水平与关节滑膜VEGF m RNA表达的影响。方法:Waster大鼠建立胶原诱导关节炎模型,连续给药2个月,各组大鼠取血,采用ELISA法检测大鼠中血...目的:观察甲氨喋吟(MTX)联合中药对胶原诱导关节炎大鼠治疗作用及对血清血管内皮细胞生长因子(VEGF)水平与关节滑膜VEGF m RNA表达的影响。方法:Waster大鼠建立胶原诱导关节炎模型,连续给药2个月,各组大鼠取血,采用ELISA法检测大鼠中血清VEGF水平,以荧光PCR法检测滑膜组织VEGF m RNA表达。结果:1血清VEGF水平:与模型组比较,各治疗组(除MTX+雷公藤组外)有显著性差异(P<0.05)。2关节滑膜VEGF m RNA表达:与对照组比较,模型组、MTX组、雷公藤组、MTX+海蛇药酒组存在显著性差异(P<0.05);与模型组比较,各组均存在显著性差异(P<0.05)。结论:胶原诱导关节炎发生与大鼠血清VEGF水平升高及关节滑膜组织VEGF m RNA表达升高有较高相关性,MTX、雷公藤及联合海蛇药酒对大鼠关节炎有一定的治疗作用。展开更多
基金Supported by the Foundation of Traditional Chinese Medicine of the Bureau of Health of Hunan Province, No. 202053
文摘AIM: To investigate the effect of Jianweiyuyang (JWYY)granule on gastric ulcer recurrence and its mechanism in the treatment of gastric ulcer in rats.METHODS: Gastric ulcer in rats was induced according to Okeba's method with minor modification and the recurrence model was induced by IL-1β. The expression of vascular endothelial growth factor mRNA (VEGF mRNA) was examined by reverse transcription polymerase chain reaction in gastric ulcer and microvessel density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry.RESULTS: MVD was higher in the JWYY treatment group (14.0±2.62) compared with the normal, model and ranitidine treatment groups (2.2±0.84, 8.8±0.97, 10.4±0.97) in rats (P＜0.01). The expression level of VEGF mRNA in gastric tissues during the healing process of JWYY treatment group rats significantly increased compared with other groups (normal group: 0.190±0.019, model group: 0.642±0.034,ranitidine group: 0.790±0.037, P＜0.01).CONCLUSION: JWYY granules can stimulate angiogenesis and enhance the expression of VEGF mRNA in gastric ulcer rats. This might be the mechanism for JWYY accelerating the ulcer healing, and preventing the recurrence of gastric ulcer.
基金This work was supported by NationalNatural Science Foundation of China (No. 39870314).
文摘Object: To localize the mRNA coding for VEGF atUltrastractural level in human breast cancer by usingdigoxigenin-labeled cDNA probes. Methods: Nonradio-active in situ hybridization at electron microscopic level was employed to detected VEGF mRNA in breast cancer.Result: Cancer cells and endothelial cell of angiogensisshow dark color in experiment sections. No dark color can be found in control sections. Positive hybridization signals showed dark dot and were located in various compartments of the breast cancer cell and endothelial cell in experiment section. No labeling was observed in control sections. In experiment sections, the staining appeared concentrated in cytoplasm and nucleus of the breast cancer cell andendothelial cell. Conclusion: Nonradioactive in situhybridization at electron microscopic level is efficient fordirect observation of the target site mRNA of VEGF in the cytoplasm and nucleus.
文摘目的:观察甲氨喋吟(MTX)联合中药对胶原诱导关节炎大鼠治疗作用及对血清血管内皮细胞生长因子(VEGF)水平与关节滑膜VEGF m RNA表达的影响。方法:Waster大鼠建立胶原诱导关节炎模型,连续给药2个月,各组大鼠取血,采用ELISA法检测大鼠中血清VEGF水平,以荧光PCR法检测滑膜组织VEGF m RNA表达。结果:1血清VEGF水平:与模型组比较,各治疗组(除MTX+雷公藤组外)有显著性差异(P<0.05)。2关节滑膜VEGF m RNA表达:与对照组比较,模型组、MTX组、雷公藤组、MTX+海蛇药酒组存在显著性差异(P<0.05);与模型组比较,各组均存在显著性差异(P<0.05)。结论:胶原诱导关节炎发生与大鼠血清VEGF水平升高及关节滑膜组织VEGF m RNA表达升高有较高相关性,MTX、雷公藤及联合海蛇药酒对大鼠关节炎有一定的治疗作用。
