In cereal species such as wheat (</span><i><span style="font-family:Verdana;">Ttiticum aestivum</span></i><span style="font-family:Verdana;">) and barley (</s...In cereal species such as wheat (</span><i><span style="font-family:Verdana;">Ttiticum aestivum</span></i><span style="font-family:Verdana;">) and barley (</span><i><span style="font-family:Verdana;">Hordeum vulg</span><span style="font-family:Verdana;">are</span></i><span><span style="font-family:Verdana;">), many studies have indicated that</span><i><span style="font-family:Verdana;"> VERNALIZATION </span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1)</span></span><span style="font-family:Verdana;"> functions as a flowering promoter, which activates florigen gene expression. The wheat florigen gene </span><i><span style="font-family:Verdana;">Wheat FLOWERING LOCUS T</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">, which is identical to </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">3) is an integrator of the vernalization, photoperiod and au</span><span><span style="font-family:Verdana;">tonomous pathways in wheat flowering, and the </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;"> expression is corre</span></span><span style="font-family:Verdana;">lated with the </span><i><span style="font-family:Verdana;">V</span><span style="font-family:Verdana;">RN</span></i><span><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">expression. </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">encodes an APETALA1/FRUITFULL-lik</span></span><span style="font-family:Verdana;">e MADS-box transcription factor which expression is induced by vernalization, leading to flowering thorough up-regulation of </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">. In </span><i><span style="font-family:Verdana;">Arabidopsis</span></i><span style="font-family:Verdana;">, it has been reported that protein-protein interactions are keys for MADS-box protein function and MADS-box transcription factors must dimerize to bind to the target gene. In this study, by using gel permeation chromatography (GPC) with purified VRN1 protein, we indicated the possibility that VRN1 protein exists as tetramer-like as flowering homeotic MADS-box proteins in </span><i><span style="font-family:Verdana;">Arabidopsis</span></i><span style="font-family:Verdana;">.展开更多
Vernalization is necessary for winter wheat to flower.However,it is unclear whether vernalization is also required for spring wheat,which is frequently sown in fall,and what molecular mechanisms underlie the vernaliza...Vernalization is necessary for winter wheat to flower.However,it is unclear whether vernalization is also required for spring wheat,which is frequently sown in fall,and what molecular mechanisms underlie the vernalization response in wheat varieties.In this study,we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat varieties.For this purpose,we determined how major vernalization genes(VRN1,VRN2,and VRN3)respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene expression.We also identified genes that are differentially regulated in response to vernalization in winter and spring wheat varieties.We found that in winter wheat,but not in spring wheat,VRN1 expression decreases when returned to warm temperature following vernalization.This finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3(H3K27me3)and tri-methylation of lysine 4 on histone H3(H3K4me3)at the VRN1 gene.Analysis of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes,including those involved in leucine catabolism,cysteine biosynthesis,and flavonoid biosynthesis.These findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.展开更多
With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology and China,the research team led by Prof.Lai LuHua(来鲁华)at BNLMS,College of Chemistry and Molecular Engi...With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology and China,the research team led by Prof.Lai LuHua(来鲁华)at BNLMS,College of Chemistry and Molecular Engineering,Peking-Tsinghua Center for Life Sciences,and Center for Quantitative Biology,Peking University recently reported that Arabidopsis transcriptional repressor VRN1undergoes liquid-liquid phase separation with DNA in Angew Chem Int Ed(2019,58:4858—4862).This research uncovers the mechanism of DNA induced VRN1phase separation and provides novel insight of phase separation mediated transcriptional repression.Zhou HuaBin,agraduate student from Lai's group,is the first author of this paper.展开更多
文摘In cereal species such as wheat (</span><i><span style="font-family:Verdana;">Ttiticum aestivum</span></i><span style="font-family:Verdana;">) and barley (</span><i><span style="font-family:Verdana;">Hordeum vulg</span><span style="font-family:Verdana;">are</span></i><span><span style="font-family:Verdana;">), many studies have indicated that</span><i><span style="font-family:Verdana;"> VERNALIZATION </span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1)</span></span><span style="font-family:Verdana;"> functions as a flowering promoter, which activates florigen gene expression. The wheat florigen gene </span><i><span style="font-family:Verdana;">Wheat FLOWERING LOCUS T</span></i><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">, which is identical to </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">3) is an integrator of the vernalization, photoperiod and au</span><span><span style="font-family:Verdana;">tonomous pathways in wheat flowering, and the </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;"> expression is corre</span></span><span style="font-family:Verdana;">lated with the </span><i><span style="font-family:Verdana;">V</span><span style="font-family:Verdana;">RN</span></i><span><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">expression. </span><i><span style="font-family:Verdana;">VRN</span></i><span style="font-family:Verdana;">1</span><i> </i><span style="font-family:Verdana;">encodes an APETALA1/FRUITFULL-lik</span></span><span style="font-family:Verdana;">e MADS-box transcription factor which expression is induced by vernalization, leading to flowering thorough up-regulation of </span><i><span style="font-family:Verdana;">WFT</span></i><span style="font-family:Verdana;">. In </span><i><span style="font-family:Verdana;">Arabidopsis</span></i><span style="font-family:Verdana;">, it has been reported that protein-protein interactions are keys for MADS-box protein function and MADS-box transcription factors must dimerize to bind to the target gene. In this study, by using gel permeation chromatography (GPC) with purified VRN1 protein, we indicated the possibility that VRN1 protein exists as tetramer-like as flowering homeotic MADS-box proteins in </span><i><span style="font-family:Verdana;">Arabidopsis</span></i><span style="font-family:Verdana;">.
基金supported by Project 2662020ZKPY002 supported by the Fundamental Research Funds for the Central Universities.
文摘Vernalization is necessary for winter wheat to flower.However,it is unclear whether vernalization is also required for spring wheat,which is frequently sown in fall,and what molecular mechanisms underlie the vernalization response in wheat varieties.In this study,we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat varieties.For this purpose,we determined how major vernalization genes(VRN1,VRN2,and VRN3)respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene expression.We also identified genes that are differentially regulated in response to vernalization in winter and spring wheat varieties.We found that in winter wheat,but not in spring wheat,VRN1 expression decreases when returned to warm temperature following vernalization.This finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3(H3K27me3)and tri-methylation of lysine 4 on histone H3(H3K4me3)at the VRN1 gene.Analysis of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes,including those involved in leucine catabolism,cysteine biosynthesis,and flavonoid biosynthesis.These findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.
文摘With the support by the National Natural Science Foundation of China and the Ministry of Science and Technology and China,the research team led by Prof.Lai LuHua(来鲁华)at BNLMS,College of Chemistry and Molecular Engineering,Peking-Tsinghua Center for Life Sciences,and Center for Quantitative Biology,Peking University recently reported that Arabidopsis transcriptional repressor VRN1undergoes liquid-liquid phase separation with DNA in Angew Chem Int Ed(2019,58:4858—4862).This research uncovers the mechanism of DNA induced VRN1phase separation and provides novel insight of phase separation mediated transcriptional repression.Zhou HuaBin,agraduate student from Lai's group,is the first author of this paper.
