目的:(1)观察补肾活血通络法对动脉粥样硬化易损斑块的稳定作用及其稳定斑块的可能机制;(2)探讨血清和斑块局部受激活调节正常T细胞表达和分泌因子(Regulated on Activated Normal T cell Expressed and Secreted factor,RANTES)水平与...目的:(1)观察补肾活血通络法对动脉粥样硬化易损斑块的稳定作用及其稳定斑块的可能机制;(2)探讨血清和斑块局部受激活调节正常T细胞表达和分泌因子(Regulated on Activated Normal T cell Expressed and Secreted factor,RANTES)水平与血管平滑肌细胞(vascular smooth muscle cells,VSMCs)凋亡程度的相关性。方法:将健康雄性新西兰兔34只,随机分成空白对照组(K组)和造模组(B组)。空白对照组8只,给予普通饲料喂养18周。造模组(B组)26只,给予高脂饲料喂养,实验开始第1周一次性注射牛血清白蛋白,实验第10周,随机抽取2只兔做病理检测证实模型组已形成粥样斑块。其余B组兔随机分为模型对照组、辛伐他汀组、健脑软脉方组。辛伐他汀组、健脑软脉方组分别予以辛伐他汀、健脑软脉方水煎剂干预,模型对照组予以生理盐水灌胃,持续8周,实验结束,于处死动物前24 h和48 h 2次给予药物触发易损斑块(中国斑点蝰蛇毒和组胺)。空白对照组(K组)按上述给药时间和部位给予同等剂量无菌生理盐水注射。实验终点处死实验兔,开胸迅速分离主动脉全长,检测各项指标。结果:模型对照组的主动脉病变程度、血脂水平、血清RANTES水平、斑块内RANTES m RAN和蛋白表达水平、VSMCs凋亡率与健脑软脉方组、辛伐他汀组、空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组与空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组之间无显著差异(P>0.05)。结论:本研究表明补肾活血通络法可能通过调节血脂水平,抑制炎症反应,抑制平滑肌细胞凋亡,从而抑制模型兔动脉粥样硬化斑块的不稳定性进展。展开更多
A new cerebroside,1-O-(β-D-glucopyranosyloxy)-(2S,3R,4E,8Z)-2-[(2′R)-2’-hydroxylignoceranoylamino]-4,8-tetradecene-3- diol was isolated from the 60%EtOH extract of traditional Chinese medical plant Cyperus rotundus...A new cerebroside,1-O-(β-D-glucopyranosyloxy)-(2S,3R,4E,8Z)-2-[(2′R)-2’-hydroxylignoceranoylamino]-4,8-tetradecene-3- diol was isolated from the 60%EtOH extract of traditional Chinese medical plant Cyperus rotundus L.Its structure was determined on the basis of spectroscopic data.This new compound showed anti-proliferation effect on vascular smooth muscle cells(VSMCs).展开更多
文摘目的:(1)观察补肾活血通络法对动脉粥样硬化易损斑块的稳定作用及其稳定斑块的可能机制;(2)探讨血清和斑块局部受激活调节正常T细胞表达和分泌因子(Regulated on Activated Normal T cell Expressed and Secreted factor,RANTES)水平与血管平滑肌细胞(vascular smooth muscle cells,VSMCs)凋亡程度的相关性。方法:将健康雄性新西兰兔34只,随机分成空白对照组(K组)和造模组(B组)。空白对照组8只,给予普通饲料喂养18周。造模组(B组)26只,给予高脂饲料喂养,实验开始第1周一次性注射牛血清白蛋白,实验第10周,随机抽取2只兔做病理检测证实模型组已形成粥样斑块。其余B组兔随机分为模型对照组、辛伐他汀组、健脑软脉方组。辛伐他汀组、健脑软脉方组分别予以辛伐他汀、健脑软脉方水煎剂干预,模型对照组予以生理盐水灌胃,持续8周,实验结束,于处死动物前24 h和48 h 2次给予药物触发易损斑块(中国斑点蝰蛇毒和组胺)。空白对照组(K组)按上述给药时间和部位给予同等剂量无菌生理盐水注射。实验终点处死实验兔,开胸迅速分离主动脉全长,检测各项指标。结果:模型对照组的主动脉病变程度、血脂水平、血清RANTES水平、斑块内RANTES m RAN和蛋白表达水平、VSMCs凋亡率与健脑软脉方组、辛伐他汀组、空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组与空白对照组相比有显著差异(P<0.05),健脑软脉方组和辛伐他汀组之间无显著差异(P>0.05)。结论:本研究表明补肾活血通络法可能通过调节血脂水平,抑制炎症反应,抑制平滑肌细胞凋亡,从而抑制模型兔动脉粥样硬化斑块的不稳定性进展。
基金supported by 2006 Great Basic Science Research Project of Jiangsu College and University(No. 06KJA36022)
文摘A new cerebroside,1-O-(β-D-glucopyranosyloxy)-(2S,3R,4E,8Z)-2-[(2′R)-2’-hydroxylignoceranoylamino]-4,8-tetradecene-3- diol was isolated from the 60%EtOH extract of traditional Chinese medical plant Cyperus rotundus L.Its structure was determined on the basis of spectroscopic data.This new compound showed anti-proliferation effect on vascular smooth muscle cells(VSMCs).
