Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by t...Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by testing the changes of vascular endothelial cell(CEC) ,nitric oxide(NO) and endothelin-1 (ET-1) in children with congenital heart disease. Methods Sixty patients展开更多
Objective:To investigate the effects of Naoxintong(NXT)capsule on vascular endothelial function and inflammatory mechanism in spontaneously hypertensive rats.Methods:A total of 100 spontaneously hypertensive rats were...Objective:To investigate the effects of Naoxintong(NXT)capsule on vascular endothelial function and inflammatory mechanism in spontaneously hypertensive rats.Methods:A total of 100 spontaneously hypertensive rats were divided into model group,positive control group,NXT low-dose group,NXT medium-dose group and NXT high-dose group.Rats in the model group were given an equal dose of normal saline once a day;the positive control group was given telmisartan 50 mg/(kg·d)once a day;the low,middle and high dose groups were given the NTX 0.5,1.0,2.0 mg/(kg·d)once a day,respectively.Rats in each group were continuously intragastrically administered for 12 weeks.The vascular endothelial function index,inflammation index and blood pressure of each group were observed at the end of 8 weeks.Results:Endothelin(ET),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),C-reactive protein(CRP)and systolic blood pressure levels were lower in the positive control group and NXT group compared with model group.In the model group,the NO level was lower than the positive control group and NXT group;the levels of ET,IL-6,TNF-α,CRP and systolic blood pressure in the middle-dose group and the high-dose group were lower than those in the positive control group and NXT low-dose group.Furthermore,the level of NO was higher in positive control group and NXT middle and high dose group compared with model group.The levels of ET,IL-6,TNF-α,CRP and systolic pressure in NXT high dose group were lower than in NXT middle dose group,while the level of NO was higher in NXT high group than in NXT middle dose group and the difference was statistically significant(p<0.05).Conclusions:NXT has obviously antihypertensive effect on spontaneously hypertensive rats,and its mechanism may be related to the improvement of vascular endothelial function and inflammatory factors.展开更多
Objective: To investigate the effects of Trimetazidine Dihydrochloride Tablets on inflammatory reaction, oxidative stress, vascular endothelial function and myocardial function in patients with coronary heart disease ...Objective: To investigate the effects of Trimetazidine Dihydrochloride Tablets on inflammatory reaction, oxidative stress, vascular endothelial function and myocardial function in patients with coronary heart disease complicated with heart failure. Methods: A total of 98 patients with coronary heart disease and heart failure who met the criteria of the study were selected as the subjects, based on the random data table they were divided into the control group (n=49) and observation group (n=49), the patients in the control group were treated with Metoprolol Tartrate Sustained-release Tablets treatment, and the patients in the observation group were treated with Metoprolol Tartrate Sustained-release Tablets combined with Trimetazidine Dihydrochloride Tablets, the levels of inflammatory reaction, oxidative stress, vascular endothelial function and myocardial function indexes were compared between the two groups before and after treatment. Results: The difference of the CRP, TNF-α, MDA, SOD, NO, ET-1, LVEF, LVEDD and LVESD levels in the two groups before treatment were not statistically significant;Compared with the levels of the two groups before treatment, the two groups of CRP, TNF-α, MDA, ET-1, LVEDD and LVESD levels after treatment were significantly decreased, and the level of the observation group after treatment was significantly lower than those levels in the control group, the difference was statistically significant;The levels of SOD, NO and LVEF of the two groups after treatment were significantly higher than those in the same group before treatment, and the observation group levels [(88.09±7.51) U/ml, (72.58±14.64) mol/L, (48.34±5.09)% ] were significantly higher than the control group [(79.44±7.27) U/ml, (61.89±11.06) mol/L, (44.19±4.58)%], the difference was statistically significant. Conclusion: Trimetazidine Dihydrochloride Tablets in the treatment of coronary heart disease with heart failure can effectively inhibit the release of inflammatory factors, improve oxidative stress state, vascular endothelial function and myocardial function, has an important clinical value.展开更多
Objective:To evaluate the effect of rosuvastatin on the vascular endothelial function and inflammatory cytokine in patients after PCI.Methods:A total of 120 patients with ACS who were admitted in our hospital from Dec...Objective:To evaluate the effect of rosuvastatin on the vascular endothelial function and inflammatory cytokine in patients after PCI.Methods:A total of 120 patients with ACS who were admitted in our hospital from December, 2014 to June, 2016 were included in the study and randomized into the observation group and the control group. PCI was performed in the two groups. The patients in the control group were given routine treatments. On this basis, the patients in the observation group were given rosuvastatin (20 mg) 3 d before operation, every night before sleep, continuously for 1 month. The efficacy was evaluated 24 h and 1 month after operation. Hs-CRP, IL-10, TNF-α, vWF, ET-1, NO, TG, TC, and LDL levels before operation, 24 h and 1 month after operation in the two groups were detected.Results:vWF and ET-1 levels 1 month after operation in the observation group were significantly lower than those in the control group, while NO was significantly higher than that in the control group. hs-CRP, IL-10, and TNF-α 24 h and 1 month after operation in the observation group were significantly lower than those in the control group. TC, TG, and LDL levels 24 h and 1 month after operation in the observation group were significantly lower than those in the control group.Conclusions:Rosuvastatin can effectively reduce the serum inflammatory cytokine level, improve the vascular endothelial function, and decrease the occurrence of thrombus and restenosis in patients with ACS after PCI.展开更多
We evaluated the effect of sitagliptin on vascular endothelial function in Japanese type 2 diabetes patients without cardiovascular disease. Subjects included 24 Japanese type 2 diabetes patients without cardiovascula...We evaluated the effect of sitagliptin on vascular endothelial function in Japanese type 2 diabetes patients without cardiovascular disease. Subjects included 24 Japanese type 2 diabetes patients without cardiovascular disease. This study was a prospective, open-label, randomized clinical trial. We divided the study subjects into 2 groups: subjects who received sitagliptin 50 mg daily (sitagliptin group, n = 12) and subjects who did not receive sitagliptin (control group, n = 12). Brachial artery flow-mediated dilation (FMD) was measured after overnight fasting. Sitagliptin administration was initiated at 1 month after enrollment in study (baseline). FMD and level of biochemical variables in the sitagliptin and control groups were measured at baseline and 3 months from baseline (3 months). We evaluated the effect of sitagliptin on vascular endothelial function by measuring FMD. FMD at 3 months was significantly higher in the sitagliptin group than in the control group (5.36% ± 2.18% vs 3.41% ± 2.29%, P = 0.040), while FMD at baseline was not significantly different between the 2 groups. In addition, FMD of the sitagliptin group at 3 months was significantly higher than that at baseline (5.36% ± 2.18% vs 3.67% ± 2.30%, P = 0.004), while no significant differences were observed in the FMD of the control group during the study period. The change in the adiponectin from baseline to 3 months was significantly higher in the sitagliptin group than that in the control group (0.82 ± 2.18 μg/mL vs 0.01 ± 0.55 μg/mL, P = 0.039). Sitagliptin improves vascular endothelial function of the brachial artery in Japanese type 2 diabetes patients without cardiovascular disease. Furthermore, elevation of adiponectin may induce reduction of endothelial dysfunction in type 2 diabetes patients treated with sitagliptin.展开更多
Objective:To explore the effect of Guhong Injection combined with atyplase on vascular endothelial function and fibrinolytic system and related factors in patients with acute cerebral infarction.Methods:To select 90 p...Objective:To explore the effect of Guhong Injection combined with atyplase on vascular endothelial function and fibrinolytic system and related factors in patients with acute cerebral infarction.Methods:To select 90 patients with acute cerebral infarction in our hospital,divided into control group(48 cases)and observation group(48 cases)randomly.Patients in the control group were treated with alteplase on the basis of routine symptomatic treatment,and the observation group was treated with Guhong injection on the basis of the control group.Before and after treatment,the serum levels of related factors were detected and compared between the two groups.Results:Before treatment,there were no significant differences in serum related factors(ET-1,PAO,H-FABP,VEGF,S100β,BDNF,CEC,Fibulin-5,vWF,P-selectin,t-PA,PAI-1)between the two groups;After treatment,the serum levels of ET-1,PAO,H-FABP,S100β,CEC,Fibulin-5,vWF,P-selectin,PAI-1 in the observation group were significantly lower than those in the control group,and the serum levels of VEGF,BDNF and t-PA were higher than those in the control group,there were significant differences between the two groups.Conclusion:Guhong injection was added to patients with acute cerebral infarction on the basis of routine symptomatic treatment and ateplase,could significantly improve the level of serum related factors,it was more conducive to the control of symptoms and rehabilitation of patients,the effect was definite,it was worth further study and application in clinic.展开更多
Objective:To investigate the effects of erythropoietin combined with percutaneous coronary intervention (PCI) on myocardial damage, vascular endothelial function and neuroendocrine hormone levels in patients with ST-s...Objective:To investigate the effects of erythropoietin combined with percutaneous coronary intervention (PCI) on myocardial damage, vascular endothelial function and neuroendocrine hormone levels in patients with ST-segment elevation myocardial infarction (STEMI).Method:From August 2015 to February 2018, 80 patients with STEMI were selected from our hospital, divided into 2 groups according to admission time, 40 cases in each group, set to observation group and control group, the observation group was treated with erythropoietin combined with PCI, and the control group was treated only with PCI, to compare the degree of myocardial damage, vascular endothelial function and neuroendocrine hormone levels after treatment. Results: At 24 h after surgery, the observed group of cardiac troponin (cTNI), creatine kinase isoenzyme (CK-MB), type I collagen carboxy terminal peptide (ICTP) and ischemic modified albumin (IMA) is lower than the control group,the cystatin-C (Cys-C) level is higher than the control group, the difference was statistically significant (P<0.05);At 1 week after surgery, the levels of endothelin-1 (ET-1) and von Willebrand factor (vWF) in the observation group were lower than the control group, and the level of nitric oxide (NO) was higher than that in the control group, the difference was statistically significant (P<0.05);At 1 week after surgery, the levels of cortisol (Cor), adrenocortical hormone (ACTH), norepinephrine (NE) and Angiotensin II (AngII) in the observation group were lower than in the control group, the difference was statistically significant (P<0.05).Conclusion: Erythropoietin combined with PCI in the treatment of STEMI patients with better clinical effect, can effectively reduce the degree of myocardial damage, protect vascular endothelial cell function, improve the body's neuroendocrine hormone levels, recommend clinical application.展开更多
Objective: To discuss the effects of minimally invasive surgery under transrectal anoscope on pain stress, gastrointestinal hormones and vascular endothelial function in patients with rectal cancer. Methods Totally 64...Objective: To discuss the effects of minimally invasive surgery under transrectal anoscope on pain stress, gastrointestinal hormones and vascular endothelial function in patients with rectal cancer. Methods Totally 64 patients with rectal cancer who underwent minimally invasive surgery under transanal anastomosis from January 2017 to December 2018 in our hospital were selected as the observation group and the retrospective analysis of clinical data was conducted. Another 64 patients who underwent traditional laparotomy at the same time in our hospital were selected as the control group. Pain stress, gastrointestinal hormones, vascular endothelial function, and tumor marker changes were compared between the two groups. Results There were no significant differences in pain stress index, gastrointestinal hormone, vascular endothelial function index and tumor marker level between the two groups before operation (P>0.05). After operation, the levels of SP, BK and PGE2 in the two groups were higher than those before surgery (P<0.05), and the levels of GAS, MTL, VIP, VEGF, MMP-9, ET-1, CEA, CA724, CA125 and CA19-9 were higher with preoperative reduction of P<0.05. The levels of SP, BK, PGE2, VEGF, MMP-9, ET-1, CEA, CA724, CA125 and CA19-9 in the observation group after operation were lower than those in the control group (P<0.05), and the GAS, MTL and VIP levels were significantly higher than the control group (P<0.05). Conclusion Minimally invasive surgery under transanal anoscope can reduce the pain stress of patients with rectal cancer, and the influence of gastrointestinal hormones is slight. It can effectively reduce the vascular endothelial function index and tumor marker level, help the immune function recovery of patients, and promote postoperative rehabilitation of patients.展开更多
Objective:To investigate the effect of low dose aspirin and salvia miltiorrhiza injection on hemorrheology, endothelial function and oxidative stress in patients with pregnancy induced hypertension.Methods: One hundre...Objective:To investigate the effect of low dose aspirin and salvia miltiorrhiza injection on hemorrheology, endothelial function and oxidative stress in patients with pregnancy induced hypertension.Methods: One hundred and eighty pregnancy-induced hypertension patients treated in our hospital from April 2015 to November 2016 were selected as study subjects and randomly divided into control group and observation group with 90 cases in each group. The control group was given routine treatment. The observation group was treated with low-dose aspirin plus Salvia miltiorrhiza in the control group. After treatment, blood rheology, vascular endothelial function and oxidative stress were measured in two groups.Results: The levels of MAP and 24 h Upro in both groups were significantly lower than those before treatment. After treatment, the MAP and 24 h Upro levels in the observation group were significantly lower than those in the control group. After treatment, the level of PT was significantly higher than that before treatment, while the level of DD and Fg was significantly lower than that before treatment. Comparing the two groups after treatment showed that the PT level, and the levels of DD and Fg were significantly lower than those in the control group;After treatment, NO levels in both groups were significantly higher than those before treatment, ET-1 and ET-1/NO levels were significantly lower than before treatment, after treatment, ET-1, NO and ET The NO level in the observation group was significantly higher than that in the control group, while ET-1 and ET-1/NO levels were significantly lower than those in the control group;Compared with those before treatment, the levels of SOD in the two groups were significantly increased and MDA significantly decreased after treatment. The SOD and MDA levels in the two groups after treatment showed that the SOD level in the observation group was significantly higher than that in the control group, while MDA level was significantly lower than that of the control group.Conclusion: The combination of low-dose aspirin and Salvia miltiorrhiza injection has significant therapeutic effect on gestational hypertension, and can effectively improve the hemorheological parameters and endothelial function of patients and reduce the oxidative stress injury in patients. It is worth further clinical promotion.展开更多
Objective:To investigate the effects of Gongliuxiao Capsule on inflammatory factors, tumor markers, sex hormones and vascular endothelial function in patients with uterine fibroids. Methods:130 patients with uterine f...Objective:To investigate the effects of Gongliuxiao Capsule on inflammatory factors, tumor markers, sex hormones and vascular endothelial function in patients with uterine fibroids. Methods:130 patients with uterine fibroids admitted to our hospital from October 2015 to March 2017 were enrolled. According to the treatment, they were divided into single treatment group and combined medication group, with 65 cases in each group. The changes of inflammatory factors, tumor markers, sex hormones and vascular endothelial function were compared before and after treatment in the two groups.Results: There were no significant differences in the levels of sex hormones, inflammatory factors, tumor markers and vascular endothelial function between the two groups (P>0.05). The levels of sex hormones E2, P, FSH and LH, the levels of inflammatory factors TNF-α, MMP-9 and IGF-1, tumor markers CA125, CA19-9 and vascular endothelial function VEGF and b-FGF were significantly lower in the two groups (P<0.05). The levels of E2, P, FSH and LH in the combined drug groupswere (154.12±16.20) pmol/L, (9.12±1.02) μg/L, (13.84±1.42) U/L and (12.77±1.36) U/L, respectively,which were significantly lower than the single drug group level (P<0.05). The levels of CA125 and CA19-9 in the combined drug group were (27.89±2.88) U/mL and (28.09±3.04) U/mL, respectively, which were significantly lower than the single drug group (P<0.05). The levels of TNF-α, MMP-9 and IGF-1 in the combined drug group were (24.16±2.63) ng/L, (159.22±16.82) μg/L and (69.75±7.09) μg/L, respectively, which were significantly lower than the single drug group(P<0.05). The levels of VEGF and b-FGF in the combination group were (138.29±14.32) μg/L and (98.59±10.02) μg/L, respectively, which were significantly lower than the single drug group (P<0.05).Conclusion:Mifepristone combined with Gongliuxiao Capsule can alleviate endocrine disorders, relieve inflammatory stress response and improve vascular endothelial function in patients with uterine fibroids. It is worthy of clinical application.展开更多
Objective:To investigate the effect of neoadjuvant chemotherapy on immune function, vascular endothelial function and LEP in patients with rectal cancer.Methods: The clinical data of 110 patients undergoing laparoscop...Objective:To investigate the effect of neoadjuvant chemotherapy on immune function, vascular endothelial function and LEP in patients with rectal cancer.Methods: The clinical data of 110 patients undergoing laparoscopic rectal cancer radical resection in our hospital were retrospectively analyzed. They were divided into control group (n=55) and study group (n=55) according to the surgical procedure. The control group was treated with laparoscopic surgery, and the study group was treated with laparoscopic surgery combined with neoadjuvant chemotherapy. The tumor markers CA242 and CA19-9, immunological function indicators C3, C4, IgA, IgG, vascular endothelial function VEGF and VEGFR-2, LEP and IGF-1 were detected at 1 day before treatment and 1 day after treatment.Results:Before treatment, serum CA242, CA19-9, C3, C4, IgA, IgG, VEGF, VEGFR-2, LEP and IGF-1 levels were not significantly different between the two groups (P<0.05). After treatment, serum CA242 and CA19-9 levels were significantly lower in the two groups (P<0.05). The levels of CA242 and CA19-9 in the study group were (15.23±1.53) IU/mL and (20.13±2.01) U/mL, which were significantly lower than the control group (P<0.05). After treatment, serum IgA and IgG levels were significantly higher in both groups, and C3 and C4 levels were significantly increased in the study group (P<0.05). The levels of C3, C4, IgA and IgG in the study group were significantly higher (1.31±0.13) g/L, (0.34±0.03) g/L, (1.78±0.18) g/L and (11.02±1.13) g/L, which were higher those in the control group (P<0.05). After treatment, serum VEGF and VEGFR-2 levels were significantly lower in the two groups (P<0.05). The levels of VEGF and VEGFR-2 in the study group were (118.15±11.86) ng/L and (92.44±9.26) ng/L, which were significantly lower than those in the control group (P<0.05). After treatment, LEP levels were significantly increased in both groups, and IGF-1 levels were significantly lower in both groups (P<0.05). The LEP of the study group was (7.51±0.71) ng/mL, which were significantly higher than that of the control group, and the level of IGF-1 was (281.47±28.86) μg/L in the study group, which were significantly lower than the control group (bothP<0.05).Conclusion:Laparoscopic rectal cancer surgery combined with neoadjuvant chemotherapy can significantly improve the vascular endothelial function of patients with rectal cancer and improve the immune function of the body. It is worthy of clinical application.展开更多
Objective:To explore the effect of partial body weight support training (PBWST) on the cardiac function, neuroendocrine function, and vascular endothelial function in patients with early heart failure.Methods: A total...Objective:To explore the effect of partial body weight support training (PBWST) on the cardiac function, neuroendocrine function, and vascular endothelial function in patients with early heart failure.Methods: A total of 98 patients with early heart failure who were admitted in our hospital were included in the study and randomized into the observation group and the control group. The patients in the control group were given routine drugs and rehabilitation. On this basis, the patients in the observation group were given PBWST. The patients in the two groups were treated for 3 months. The changes of cardiac function, neuroendocrine function, and vascular endothelial function indicators before and after rehabilitation in the two groups were detected.Results: LVESD and ESV after training in the two groups were significantly reduced. LVESD and ESV after training in the observation group were significantly lower than those in the control group. SV, CI, LVEF, and FS after training in the observation group were significantly elevated when compared with before training. LVEF and FS after training in the observation group were significantly higher than those in the control group. LVEF after training in the control group was significantly elevated. PRA, AngⅡ, ET, and ALD levels after training in the observation group were significantly reduced, while NO was significantly elevated, and the improvement of the above indicators was significantly superior to that in the control group.Conclusions: PBWST can improve the cardiac function in patients with early heart failure, inhibit the excessive activation of neuroendocrine system, and protect the vascular endothelial function in order to enhance the clinical efficacy.展开更多
Objective: To investigate the effect of sufentanil on hemodynamics, oxidative stress and vascular endothelial function in patients undergoing laparoscopic cholecystectomy. Methods:A total of 88 patients undergoing lap...Objective: To investigate the effect of sufentanil on hemodynamics, oxidative stress and vascular endothelial function in patients undergoing laparoscopic cholecystectomy. Methods:A total of 88 patients undergoing laparoscopic cholecystectomy were divided into control group (n=44) and observation group (n=44) according to random data table, the patients in the control group received fentanyl anesthesia, and the observation group patients were given sufentanil anesthesia, the levels of hemodynamic, oxidative stress and vascular endothelial function at three times before anesthesia induction (T0), pneumoperitoneum (T1) and at the end of surgery (T2) between the two groups were compared. Results: At T0 moment, the levels of T0, SBP, HR, DBP, CAT, SOD, NO, MDA and ET-1 in the two groups were not statistically significant;Compared with the level of T0 moment, the levels of HR, SBP, DBP, and MDA in the two groups at T1 and T2 moment were increased in different degrees, and the levels of T1 and T2 in the observation group were significantly lower than those in the control group;The levels of CAT and SOD in the two groups of T1 and T2 moment were lower than T0 moments in different degrees, and the observation group at two time levels were significantly higher than the control group;The levels of NO and ET-1 in the two groups of T1 moment were significantly increased, and in the observation group the level of NO was significantly higher and ET-1 was significantly lower than that in the control group, all differences were statistically significant;There was no significant difference of the NO and ET-1 levels at T2 between the two groups. Conclusion: Compared with fentanyl, sufentanil has less influence on hemodynamics in laparoscopic cholecystectomy. It can effectively reduce oxidative stress and protect vascular endothelial function, and is more conducive to recovery.展开更多
Objective:To explore the effect of Huangqi injection in combined with irbesartan on the vascular endothelial function and calcitonin gene-related peptide (CGRP) in patients with early diabetic nephropathy (DN). Method...Objective:To explore the effect of Huangqi injection in combined with irbesartan on the vascular endothelial function and calcitonin gene-related peptide (CGRP) in patients with early diabetic nephropathy (DN). Methods:A total of 110 patients with early DN who were admitted in our hospital from August, 2015 to August, 2016 were included in the study and randomized into the observation group and the control group, 55 in each group. On the basis of routine treatments, the patients in the control group were given irbesartan (150 mg/time), 1 time/d, and given additional diuretics and calcium channel block when the blood pressure was greater than 130/80 mmHg. On the above basis, the patients in the control group were given Huangqi injection (50 mL)+0.9%NaCl (250 mL), ivdrip, 1 time/d. After 1-month treatment, the efficacy was evaluated. The full automatic biochemical analyzer was used to detect Scr, Bun, UAER, 24 h Upor, ET-1, TXB2, NO, and CGRP before and after treatment in the two groups. ELISA was used to detect PCX and LAP. Results:Scr, Bun, UAER, 24 h Upor levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). ET-1 and TXB2 levels after treatment in the observation group were significantly lower than those in the control group (P<0.05), while NO and CGRP levels were significantly higher than those in the control group (P<0.05). PCX and LAP levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). Conclusions:Huangqi injection in combined with irbesartan can effectively reduce the urine protein in patients with early DN, regulate the vascular endothelial function, and improve the renal function in order to delay the development of DN.展开更多
Objective: To investigate effect of aerobic exercise training combined with conventional drug on red blood cell parameters, vascular endothelial function and cardiac function in elderly patients with coronary heart di...Objective: To investigate effect of aerobic exercise training combined with conventional drug on red blood cell parameters, vascular endothelial function and cardiac function in elderly patients with coronary heart disease complicated with chronic heart failure. Methods: A total of 110 elderly patients with coronary heart disease and chronic heart failure according to random data table were divided into control group (n=54) and observation group (n=56). The patients in control group were treated with conventional drug, and patients in the observation group received conventional drug combined with aerobic exercise training. Before and after treatment, levels of red blood cell parameters, vascular endothelial function and cardiac function indexes were compared between the two groups. Results: Before treatment, the difference of HCT, RDW, RBC, NO, ET-1, LVEF, LVEDD and LVESD levels in the two groups were not significantly. After treatment, HCT and RBC levels in two groups were no statistically significant difference;The levels of RDW, ET-1, LVEDD and LVESD in observation group were significantly lower than those in this group before treatment, and significantly lower than those in the control group after treatment;The levels of NO and LVEF in two groups were significantly higher than those in the group before treatment, and levels of NO and LVEF in the observation group were significantly higher than those in control group after treatment. Conclusion: The clinical effect of aerobic exercise training combined with conventional drug in treatment of senile coronary heart disease with chronic heart failure was significant, which can effectively increase the RDW level of patients, improve vascular endothelial function and heart function, it has important clinical value.展开更多
Objective: To investigate the effect of telmisartan combined with Nifedipine Controlled Release Tablet on inflammatory factors, vascular endothelial function and left ventricular function in patients with coronary hea...Objective: To investigate the effect of telmisartan combined with Nifedipine Controlled Release Tablet on inflammatory factors, vascular endothelial function and left ventricular function in patients with coronary heart disease with mild to moderate hypertension. Methods:A total of 92 cases of patients with coronary heart disease with mild to moderate hypertension were selected as the object of observation, according to the random data table, they were divided into the control group (n=46) and observation group (n=46), and patients in the control group were treated with Nifedipine Controlled Release Table therapy, on this basis, the observation group patients were given telmisartan treatment, two groups were treated for 6 months. The levels of the blood pressure, inflammatory factors, vascular endothelial function and left ventricular function compared between the two groups before and after treatment. Results: There were no significant differences in the levels of SBP, DBP, hs-CRP, TNF-α, NO, ET-1, LVEF, LVEDD and LVESD in the two groups before treatment. After treatment, two groups of SBP, DBP, hs-CRP, TNF-α, ET-1, LVEDD and LVESD levels were significantly lower than those in the same group before treatment, and after treatment, the levels of SBP, DBP, hs-CRP, TNF-α, ET-1 and LVESD in the observation group were significantly lower than those in the control group, while there were no significant difference in the level of LVEDD between the two groups after treatment;Compared with level in the group before treatment, the levels of NO and LVEF in the two groups were significantly increased, and the observation group [(82.13±19.01) μmol/L, (52.83±7.45)%] was significantly higher than the control group ((67.37±13.08) μmol/L, (49.47±6.96)%)Conclusion: Telmisartan combined with Nifedipine Controlled Release Table in treating coronary heart disease with mild to moderate hypertension, can effectively control blood pressure, reduce the inflammatory stress, improve vascular endothelial function and left ventricular function of patients, has an important clinical value.展开更多
Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases,...Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases, having one or more than two branches narrow ( ≥70% ) , were diagnosed by coronary angiography. These patients were divided randomly into carvedilol group (n = 28) and control group (n = 23) who did not take carvedilol.Endothelin (ET) and nitro dioxide (NO) levels of peripheral blood were measured before and after PTCA,before and after two weeks by taking carvedilol. Resuits Compared with the ET and NO levels before PTCA, ET were markedly increased and NO were decreased after PTCA (p <0.05 ) ; compared with the ET and NO levels before taking carvedilol, ET were decreased and NO were increased after two week (p <0.05) , but the ET and NO levels of the control group did not change in the period of two weeks observation(p > 0.05). Conclusions Carvedilol may improve the coronary vascular endothelial function after PTCA.展开更多
OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese vo...OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese volunteers with the phlegmdampness constitution or balanced constitution were recruited for this study respectively.The clinical baseline data was collected,and the vascular endothelial function was evaluated using the EndoPAT?.Blood samples were collected for the serum proteome analysis.The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis,correlation analysis,receiver operating characteristic(ROC)curve analysis,and random forest model investigation were conducted.RESULTS:There were no statistical differences found in the baseline data.For vascular endothelial function,the reactive hyperemia index(RHI)of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group(1.46±0.30 vs 2.82±0.78,P<0.0001),indicating vascular endothelial dysfunction.There are 66 differentially expressed serum proteins between the two groups.apolipoprotein A2(Apo A2),angiotensin-converting enzyme 2(ACE-2),interleukin-33(IL-33),and forkhead box P3(FoxP3)showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.CONCLUSION:Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group.Thus,alterations in the expression levels of key serum proteins,including Apo A2,ACE-2,IL-33,and Fox P3 could serve as potential biomarkers in the obese population at risk of CVD.展开更多
BACKGROUND The incidence and mortality of colorectal cancer(CRC)are among the highest in the world,and its occurrence and development are closely related to tumor neovascularization.When the balance between pigment ep...BACKGROUND The incidence and mortality of colorectal cancer(CRC)are among the highest in the world,and its occurrence and development are closely related to tumor neovascularization.When the balance between pigment epithelium-derived factors(PEDF)that inhibit angiogenesis and vascular endothelial growth factors(VEGF)that stimulate angiogenesis is broken,angiogenesis is out of control,resulting in tumor development.Therefore,it is very necessary to find more therapeutic targets for CRC for early intervention and later treatment.AIM To investigate the expression and significance of PEDF,VEGF,and CD31-stained microvessel density values(CD31-MVD)in normal colorectal mucosa,adenoma,and CRC.METHODS In this case-control study,we collected archived wax blocks of specimens from the Digestive Endoscopy Center and the General Surgery Department of Chengdu Second People's Hospital from April 2022 to October 2022.Fifty cases of specimen wax blocks were selected as normal intestinal mucosa confirmed by electronic colonoscopy and concurrent biopsy(normal control group),50 cases of specimen wax blocks were selected as colorectal adenoma confirmed by electronic colonoscopy and pathological biopsy(adenoma group),and 50 cases of specimen wax blocks were selected as CRC confirmed by postoperative pathological biopsy after inpatient operation of general surgery(CRC group).An immunohistochemical staining experiment was carried out to detect PEDF and VEGF expression in three groups of specimens,analyze their differences,study the relationship between the two and clinicopathological factors in CRC group,record CD31-MVD in the three groups,and analyze the correlation of PEDF,VEGF,and CD31-MVD in the colorectal adenoma group and the CRC group.The F test or adjusted F test is used to analyze measurement data statistically.Kruskal-Wallis rank sum test was used between groups for ranked data.The chi-square test,adjusted chi-square test,or Fisher's exact test were used to compare the rates between groups.All differences between groups were compared using the Bonferroni method for multiple comparisons.Spearman correlation analysis was used to test the correlation of the data.The test level(α)was 0.05,and a two-sided P<0.05 was considered statistically significant.RESULTS The positive expression rate and expression intensity of PEDF were gradually decreased in the normal control group,adenoma group,and CRC group(100%vs 78%vs 50%,χ^(2)=34.430,P<0.001;++~++vs+~++vs-~+,H=94.059,P<0.001),while VEGF increased gradually(0%vs 68%vs 96%,χ^(2)=98.35,P<0.001;-vs-~+vs++~+++,H=107.734,P<0.001).In the CRC group,the positive expression rate of PEDF decreased with the increase of differen-tiation degree,invasion depth,lymph node metastasis,distant metastasis,and TNM stage(χ^(2)=20.513,4.160,5.128,6.349,5.128,P<0.05);the high expression rate of VEGF was the opposite(χ^(2)=10.317,13.134,17.643,21.844,17.643,P<0.05).In the colorectal adenoma group,the expression intensity of PEDF correlated negatively with CD31-MVD(r=-0.601,P<0.001),whereas VEGF was not significantly different(r=0.258,P=0.07).In the CRC group,the expression intensity of PEDF correlated negatively with the expression intensity of CD31-MVD and VEGF(r=-0.297,P<0.05;r=-0.548,P<0.05),while VEGF expression intensity was positively related to CD31-MVD(r=0.421,P=0.002).CONCLUSION It is possible that PEDF can be used as a new treatment and prevention target for CRC by upregulating the expression of PEDF while inhibiting the expression of VEGF.展开更多
The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic mac...The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).展开更多
文摘Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by testing the changes of vascular endothelial cell(CEC) ,nitric oxide(NO) and endothelin-1 (ET-1) in children with congenital heart disease. Methods Sixty patients
文摘Objective:To investigate the effects of Naoxintong(NXT)capsule on vascular endothelial function and inflammatory mechanism in spontaneously hypertensive rats.Methods:A total of 100 spontaneously hypertensive rats were divided into model group,positive control group,NXT low-dose group,NXT medium-dose group and NXT high-dose group.Rats in the model group were given an equal dose of normal saline once a day;the positive control group was given telmisartan 50 mg/(kg·d)once a day;the low,middle and high dose groups were given the NTX 0.5,1.0,2.0 mg/(kg·d)once a day,respectively.Rats in each group were continuously intragastrically administered for 12 weeks.The vascular endothelial function index,inflammation index and blood pressure of each group were observed at the end of 8 weeks.Results:Endothelin(ET),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),C-reactive protein(CRP)and systolic blood pressure levels were lower in the positive control group and NXT group compared with model group.In the model group,the NO level was lower than the positive control group and NXT group;the levels of ET,IL-6,TNF-α,CRP and systolic blood pressure in the middle-dose group and the high-dose group were lower than those in the positive control group and NXT low-dose group.Furthermore,the level of NO was higher in positive control group and NXT middle and high dose group compared with model group.The levels of ET,IL-6,TNF-α,CRP and systolic pressure in NXT high dose group were lower than in NXT middle dose group,while the level of NO was higher in NXT high group than in NXT middle dose group and the difference was statistically significant(p<0.05).Conclusions:NXT has obviously antihypertensive effect on spontaneously hypertensive rats,and its mechanism may be related to the improvement of vascular endothelial function and inflammatory factors.
文摘Objective: To investigate the effects of Trimetazidine Dihydrochloride Tablets on inflammatory reaction, oxidative stress, vascular endothelial function and myocardial function in patients with coronary heart disease complicated with heart failure. Methods: A total of 98 patients with coronary heart disease and heart failure who met the criteria of the study were selected as the subjects, based on the random data table they were divided into the control group (n=49) and observation group (n=49), the patients in the control group were treated with Metoprolol Tartrate Sustained-release Tablets treatment, and the patients in the observation group were treated with Metoprolol Tartrate Sustained-release Tablets combined with Trimetazidine Dihydrochloride Tablets, the levels of inflammatory reaction, oxidative stress, vascular endothelial function and myocardial function indexes were compared between the two groups before and after treatment. Results: The difference of the CRP, TNF-α, MDA, SOD, NO, ET-1, LVEF, LVEDD and LVESD levels in the two groups before treatment were not statistically significant;Compared with the levels of the two groups before treatment, the two groups of CRP, TNF-α, MDA, ET-1, LVEDD and LVESD levels after treatment were significantly decreased, and the level of the observation group after treatment was significantly lower than those levels in the control group, the difference was statistically significant;The levels of SOD, NO and LVEF of the two groups after treatment were significantly higher than those in the same group before treatment, and the observation group levels [(88.09±7.51) U/ml, (72.58±14.64) mol/L, (48.34±5.09)% ] were significantly higher than the control group [(79.44±7.27) U/ml, (61.89±11.06) mol/L, (44.19±4.58)%], the difference was statistically significant. Conclusion: Trimetazidine Dihydrochloride Tablets in the treatment of coronary heart disease with heart failure can effectively inhibit the release of inflammatory factors, improve oxidative stress state, vascular endothelial function and myocardial function, has an important clinical value.
文摘Objective:To evaluate the effect of rosuvastatin on the vascular endothelial function and inflammatory cytokine in patients after PCI.Methods:A total of 120 patients with ACS who were admitted in our hospital from December, 2014 to June, 2016 were included in the study and randomized into the observation group and the control group. PCI was performed in the two groups. The patients in the control group were given routine treatments. On this basis, the patients in the observation group were given rosuvastatin (20 mg) 3 d before operation, every night before sleep, continuously for 1 month. The efficacy was evaluated 24 h and 1 month after operation. Hs-CRP, IL-10, TNF-α, vWF, ET-1, NO, TG, TC, and LDL levels before operation, 24 h and 1 month after operation in the two groups were detected.Results:vWF and ET-1 levels 1 month after operation in the observation group were significantly lower than those in the control group, while NO was significantly higher than that in the control group. hs-CRP, IL-10, and TNF-α 24 h and 1 month after operation in the observation group were significantly lower than those in the control group. TC, TG, and LDL levels 24 h and 1 month after operation in the observation group were significantly lower than those in the control group.Conclusions:Rosuvastatin can effectively reduce the serum inflammatory cytokine level, improve the vascular endothelial function, and decrease the occurrence of thrombus and restenosis in patients with ACS after PCI.
文摘We evaluated the effect of sitagliptin on vascular endothelial function in Japanese type 2 diabetes patients without cardiovascular disease. Subjects included 24 Japanese type 2 diabetes patients without cardiovascular disease. This study was a prospective, open-label, randomized clinical trial. We divided the study subjects into 2 groups: subjects who received sitagliptin 50 mg daily (sitagliptin group, n = 12) and subjects who did not receive sitagliptin (control group, n = 12). Brachial artery flow-mediated dilation (FMD) was measured after overnight fasting. Sitagliptin administration was initiated at 1 month after enrollment in study (baseline). FMD and level of biochemical variables in the sitagliptin and control groups were measured at baseline and 3 months from baseline (3 months). We evaluated the effect of sitagliptin on vascular endothelial function by measuring FMD. FMD at 3 months was significantly higher in the sitagliptin group than in the control group (5.36% ± 2.18% vs 3.41% ± 2.29%, P = 0.040), while FMD at baseline was not significantly different between the 2 groups. In addition, FMD of the sitagliptin group at 3 months was significantly higher than that at baseline (5.36% ± 2.18% vs 3.67% ± 2.30%, P = 0.004), while no significant differences were observed in the FMD of the control group during the study period. The change in the adiponectin from baseline to 3 months was significantly higher in the sitagliptin group than that in the control group (0.82 ± 2.18 μg/mL vs 0.01 ± 0.55 μg/mL, P = 0.039). Sitagliptin improves vascular endothelial function of the brachial artery in Japanese type 2 diabetes patients without cardiovascular disease. Furthermore, elevation of adiponectin may induce reduction of endothelial dysfunction in type 2 diabetes patients treated with sitagliptin.
文摘Objective:To explore the effect of Guhong Injection combined with atyplase on vascular endothelial function and fibrinolytic system and related factors in patients with acute cerebral infarction.Methods:To select 90 patients with acute cerebral infarction in our hospital,divided into control group(48 cases)and observation group(48 cases)randomly.Patients in the control group were treated with alteplase on the basis of routine symptomatic treatment,and the observation group was treated with Guhong injection on the basis of the control group.Before and after treatment,the serum levels of related factors were detected and compared between the two groups.Results:Before treatment,there were no significant differences in serum related factors(ET-1,PAO,H-FABP,VEGF,S100β,BDNF,CEC,Fibulin-5,vWF,P-selectin,t-PA,PAI-1)between the two groups;After treatment,the serum levels of ET-1,PAO,H-FABP,S100β,CEC,Fibulin-5,vWF,P-selectin,PAI-1 in the observation group were significantly lower than those in the control group,and the serum levels of VEGF,BDNF and t-PA were higher than those in the control group,there were significant differences between the two groups.Conclusion:Guhong injection was added to patients with acute cerebral infarction on the basis of routine symptomatic treatment and ateplase,could significantly improve the level of serum related factors,it was more conducive to the control of symptoms and rehabilitation of patients,the effect was definite,it was worth further study and application in clinic.
文摘Objective:To investigate the effects of erythropoietin combined with percutaneous coronary intervention (PCI) on myocardial damage, vascular endothelial function and neuroendocrine hormone levels in patients with ST-segment elevation myocardial infarction (STEMI).Method:From August 2015 to February 2018, 80 patients with STEMI were selected from our hospital, divided into 2 groups according to admission time, 40 cases in each group, set to observation group and control group, the observation group was treated with erythropoietin combined with PCI, and the control group was treated only with PCI, to compare the degree of myocardial damage, vascular endothelial function and neuroendocrine hormone levels after treatment. Results: At 24 h after surgery, the observed group of cardiac troponin (cTNI), creatine kinase isoenzyme (CK-MB), type I collagen carboxy terminal peptide (ICTP) and ischemic modified albumin (IMA) is lower than the control group,the cystatin-C (Cys-C) level is higher than the control group, the difference was statistically significant (P<0.05);At 1 week after surgery, the levels of endothelin-1 (ET-1) and von Willebrand factor (vWF) in the observation group were lower than the control group, and the level of nitric oxide (NO) was higher than that in the control group, the difference was statistically significant (P<0.05);At 1 week after surgery, the levels of cortisol (Cor), adrenocortical hormone (ACTH), norepinephrine (NE) and Angiotensin II (AngII) in the observation group were lower than in the control group, the difference was statistically significant (P<0.05).Conclusion: Erythropoietin combined with PCI in the treatment of STEMI patients with better clinical effect, can effectively reduce the degree of myocardial damage, protect vascular endothelial cell function, improve the body's neuroendocrine hormone levels, recommend clinical application.
基金Chongqing Municipal Health Planning Commission Scientific Research Project of 2017(2017MSXM194).
文摘Objective: To discuss the effects of minimally invasive surgery under transrectal anoscope on pain stress, gastrointestinal hormones and vascular endothelial function in patients with rectal cancer. Methods Totally 64 patients with rectal cancer who underwent minimally invasive surgery under transanal anastomosis from January 2017 to December 2018 in our hospital were selected as the observation group and the retrospective analysis of clinical data was conducted. Another 64 patients who underwent traditional laparotomy at the same time in our hospital were selected as the control group. Pain stress, gastrointestinal hormones, vascular endothelial function, and tumor marker changes were compared between the two groups. Results There were no significant differences in pain stress index, gastrointestinal hormone, vascular endothelial function index and tumor marker level between the two groups before operation (P>0.05). After operation, the levels of SP, BK and PGE2 in the two groups were higher than those before surgery (P<0.05), and the levels of GAS, MTL, VIP, VEGF, MMP-9, ET-1, CEA, CA724, CA125 and CA19-9 were higher with preoperative reduction of P<0.05. The levels of SP, BK, PGE2, VEGF, MMP-9, ET-1, CEA, CA724, CA125 and CA19-9 in the observation group after operation were lower than those in the control group (P<0.05), and the GAS, MTL and VIP levels were significantly higher than the control group (P<0.05). Conclusion Minimally invasive surgery under transanal anoscope can reduce the pain stress of patients with rectal cancer, and the influence of gastrointestinal hormones is slight. It can effectively reduce the vascular endothelial function index and tumor marker level, help the immune function recovery of patients, and promote postoperative rehabilitation of patients.
文摘Objective:To investigate the effect of low dose aspirin and salvia miltiorrhiza injection on hemorrheology, endothelial function and oxidative stress in patients with pregnancy induced hypertension.Methods: One hundred and eighty pregnancy-induced hypertension patients treated in our hospital from April 2015 to November 2016 were selected as study subjects and randomly divided into control group and observation group with 90 cases in each group. The control group was given routine treatment. The observation group was treated with low-dose aspirin plus Salvia miltiorrhiza in the control group. After treatment, blood rheology, vascular endothelial function and oxidative stress were measured in two groups.Results: The levels of MAP and 24 h Upro in both groups were significantly lower than those before treatment. After treatment, the MAP and 24 h Upro levels in the observation group were significantly lower than those in the control group. After treatment, the level of PT was significantly higher than that before treatment, while the level of DD and Fg was significantly lower than that before treatment. Comparing the two groups after treatment showed that the PT level, and the levels of DD and Fg were significantly lower than those in the control group;After treatment, NO levels in both groups were significantly higher than those before treatment, ET-1 and ET-1/NO levels were significantly lower than before treatment, after treatment, ET-1, NO and ET The NO level in the observation group was significantly higher than that in the control group, while ET-1 and ET-1/NO levels were significantly lower than those in the control group;Compared with those before treatment, the levels of SOD in the two groups were significantly increased and MDA significantly decreased after treatment. The SOD and MDA levels in the two groups after treatment showed that the SOD level in the observation group was significantly higher than that in the control group, while MDA level was significantly lower than that of the control group.Conclusion: The combination of low-dose aspirin and Salvia miltiorrhiza injection has significant therapeutic effect on gestational hypertension, and can effectively improve the hemorheological parameters and endothelial function of patients and reduce the oxidative stress injury in patients. It is worth further clinical promotion.
文摘Objective:To investigate the effects of Gongliuxiao Capsule on inflammatory factors, tumor markers, sex hormones and vascular endothelial function in patients with uterine fibroids. Methods:130 patients with uterine fibroids admitted to our hospital from October 2015 to March 2017 were enrolled. According to the treatment, they were divided into single treatment group and combined medication group, with 65 cases in each group. The changes of inflammatory factors, tumor markers, sex hormones and vascular endothelial function were compared before and after treatment in the two groups.Results: There were no significant differences in the levels of sex hormones, inflammatory factors, tumor markers and vascular endothelial function between the two groups (P>0.05). The levels of sex hormones E2, P, FSH and LH, the levels of inflammatory factors TNF-α, MMP-9 and IGF-1, tumor markers CA125, CA19-9 and vascular endothelial function VEGF and b-FGF were significantly lower in the two groups (P<0.05). The levels of E2, P, FSH and LH in the combined drug groupswere (154.12±16.20) pmol/L, (9.12±1.02) μg/L, (13.84±1.42) U/L and (12.77±1.36) U/L, respectively,which were significantly lower than the single drug group level (P<0.05). The levels of CA125 and CA19-9 in the combined drug group were (27.89±2.88) U/mL and (28.09±3.04) U/mL, respectively, which were significantly lower than the single drug group (P<0.05). The levels of TNF-α, MMP-9 and IGF-1 in the combined drug group were (24.16±2.63) ng/L, (159.22±16.82) μg/L and (69.75±7.09) μg/L, respectively, which were significantly lower than the single drug group(P<0.05). The levels of VEGF and b-FGF in the combination group were (138.29±14.32) μg/L and (98.59±10.02) μg/L, respectively, which were significantly lower than the single drug group (P<0.05).Conclusion:Mifepristone combined with Gongliuxiao Capsule can alleviate endocrine disorders, relieve inflammatory stress response and improve vascular endothelial function in patients with uterine fibroids. It is worthy of clinical application.
文摘Objective:To investigate the effect of neoadjuvant chemotherapy on immune function, vascular endothelial function and LEP in patients with rectal cancer.Methods: The clinical data of 110 patients undergoing laparoscopic rectal cancer radical resection in our hospital were retrospectively analyzed. They were divided into control group (n=55) and study group (n=55) according to the surgical procedure. The control group was treated with laparoscopic surgery, and the study group was treated with laparoscopic surgery combined with neoadjuvant chemotherapy. The tumor markers CA242 and CA19-9, immunological function indicators C3, C4, IgA, IgG, vascular endothelial function VEGF and VEGFR-2, LEP and IGF-1 were detected at 1 day before treatment and 1 day after treatment.Results:Before treatment, serum CA242, CA19-9, C3, C4, IgA, IgG, VEGF, VEGFR-2, LEP and IGF-1 levels were not significantly different between the two groups (P<0.05). After treatment, serum CA242 and CA19-9 levels were significantly lower in the two groups (P<0.05). The levels of CA242 and CA19-9 in the study group were (15.23±1.53) IU/mL and (20.13±2.01) U/mL, which were significantly lower than the control group (P<0.05). After treatment, serum IgA and IgG levels were significantly higher in both groups, and C3 and C4 levels were significantly increased in the study group (P<0.05). The levels of C3, C4, IgA and IgG in the study group were significantly higher (1.31±0.13) g/L, (0.34±0.03) g/L, (1.78±0.18) g/L and (11.02±1.13) g/L, which were higher those in the control group (P<0.05). After treatment, serum VEGF and VEGFR-2 levels were significantly lower in the two groups (P<0.05). The levels of VEGF and VEGFR-2 in the study group were (118.15±11.86) ng/L and (92.44±9.26) ng/L, which were significantly lower than those in the control group (P<0.05). After treatment, LEP levels were significantly increased in both groups, and IGF-1 levels were significantly lower in both groups (P<0.05). The LEP of the study group was (7.51±0.71) ng/mL, which were significantly higher than that of the control group, and the level of IGF-1 was (281.47±28.86) μg/L in the study group, which were significantly lower than the control group (bothP<0.05).Conclusion:Laparoscopic rectal cancer surgery combined with neoadjuvant chemotherapy can significantly improve the vascular endothelial function of patients with rectal cancer and improve the immune function of the body. It is worthy of clinical application.
文摘Objective:To explore the effect of partial body weight support training (PBWST) on the cardiac function, neuroendocrine function, and vascular endothelial function in patients with early heart failure.Methods: A total of 98 patients with early heart failure who were admitted in our hospital were included in the study and randomized into the observation group and the control group. The patients in the control group were given routine drugs and rehabilitation. On this basis, the patients in the observation group were given PBWST. The patients in the two groups were treated for 3 months. The changes of cardiac function, neuroendocrine function, and vascular endothelial function indicators before and after rehabilitation in the two groups were detected.Results: LVESD and ESV after training in the two groups were significantly reduced. LVESD and ESV after training in the observation group were significantly lower than those in the control group. SV, CI, LVEF, and FS after training in the observation group were significantly elevated when compared with before training. LVEF and FS after training in the observation group were significantly higher than those in the control group. LVEF after training in the control group was significantly elevated. PRA, AngⅡ, ET, and ALD levels after training in the observation group were significantly reduced, while NO was significantly elevated, and the improvement of the above indicators was significantly superior to that in the control group.Conclusions: PBWST can improve the cardiac function in patients with early heart failure, inhibit the excessive activation of neuroendocrine system, and protect the vascular endothelial function in order to enhance the clinical efficacy.
文摘Objective: To investigate the effect of sufentanil on hemodynamics, oxidative stress and vascular endothelial function in patients undergoing laparoscopic cholecystectomy. Methods:A total of 88 patients undergoing laparoscopic cholecystectomy were divided into control group (n=44) and observation group (n=44) according to random data table, the patients in the control group received fentanyl anesthesia, and the observation group patients were given sufentanil anesthesia, the levels of hemodynamic, oxidative stress and vascular endothelial function at three times before anesthesia induction (T0), pneumoperitoneum (T1) and at the end of surgery (T2) between the two groups were compared. Results: At T0 moment, the levels of T0, SBP, HR, DBP, CAT, SOD, NO, MDA and ET-1 in the two groups were not statistically significant;Compared with the level of T0 moment, the levels of HR, SBP, DBP, and MDA in the two groups at T1 and T2 moment were increased in different degrees, and the levels of T1 and T2 in the observation group were significantly lower than those in the control group;The levels of CAT and SOD in the two groups of T1 and T2 moment were lower than T0 moments in different degrees, and the observation group at two time levels were significantly higher than the control group;The levels of NO and ET-1 in the two groups of T1 moment were significantly increased, and in the observation group the level of NO was significantly higher and ET-1 was significantly lower than that in the control group, all differences were statistically significant;There was no significant difference of the NO and ET-1 levels at T2 between the two groups. Conclusion: Compared with fentanyl, sufentanil has less influence on hemodynamics in laparoscopic cholecystectomy. It can effectively reduce oxidative stress and protect vascular endothelial function, and is more conducive to recovery.
文摘Objective:To explore the effect of Huangqi injection in combined with irbesartan on the vascular endothelial function and calcitonin gene-related peptide (CGRP) in patients with early diabetic nephropathy (DN). Methods:A total of 110 patients with early DN who were admitted in our hospital from August, 2015 to August, 2016 were included in the study and randomized into the observation group and the control group, 55 in each group. On the basis of routine treatments, the patients in the control group were given irbesartan (150 mg/time), 1 time/d, and given additional diuretics and calcium channel block when the blood pressure was greater than 130/80 mmHg. On the above basis, the patients in the control group were given Huangqi injection (50 mL)+0.9%NaCl (250 mL), ivdrip, 1 time/d. After 1-month treatment, the efficacy was evaluated. The full automatic biochemical analyzer was used to detect Scr, Bun, UAER, 24 h Upor, ET-1, TXB2, NO, and CGRP before and after treatment in the two groups. ELISA was used to detect PCX and LAP. Results:Scr, Bun, UAER, 24 h Upor levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). ET-1 and TXB2 levels after treatment in the observation group were significantly lower than those in the control group (P<0.05), while NO and CGRP levels were significantly higher than those in the control group (P<0.05). PCX and LAP levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). Conclusions:Huangqi injection in combined with irbesartan can effectively reduce the urine protein in patients with early DN, regulate the vascular endothelial function, and improve the renal function in order to delay the development of DN.
文摘Objective: To investigate effect of aerobic exercise training combined with conventional drug on red blood cell parameters, vascular endothelial function and cardiac function in elderly patients with coronary heart disease complicated with chronic heart failure. Methods: A total of 110 elderly patients with coronary heart disease and chronic heart failure according to random data table were divided into control group (n=54) and observation group (n=56). The patients in control group were treated with conventional drug, and patients in the observation group received conventional drug combined with aerobic exercise training. Before and after treatment, levels of red blood cell parameters, vascular endothelial function and cardiac function indexes were compared between the two groups. Results: Before treatment, the difference of HCT, RDW, RBC, NO, ET-1, LVEF, LVEDD and LVESD levels in the two groups were not significantly. After treatment, HCT and RBC levels in two groups were no statistically significant difference;The levels of RDW, ET-1, LVEDD and LVESD in observation group were significantly lower than those in this group before treatment, and significantly lower than those in the control group after treatment;The levels of NO and LVEF in two groups were significantly higher than those in the group before treatment, and levels of NO and LVEF in the observation group were significantly higher than those in control group after treatment. Conclusion: The clinical effect of aerobic exercise training combined with conventional drug in treatment of senile coronary heart disease with chronic heart failure was significant, which can effectively increase the RDW level of patients, improve vascular endothelial function and heart function, it has important clinical value.
文摘Objective: To investigate the effect of telmisartan combined with Nifedipine Controlled Release Tablet on inflammatory factors, vascular endothelial function and left ventricular function in patients with coronary heart disease with mild to moderate hypertension. Methods:A total of 92 cases of patients with coronary heart disease with mild to moderate hypertension were selected as the object of observation, according to the random data table, they were divided into the control group (n=46) and observation group (n=46), and patients in the control group were treated with Nifedipine Controlled Release Table therapy, on this basis, the observation group patients were given telmisartan treatment, two groups were treated for 6 months. The levels of the blood pressure, inflammatory factors, vascular endothelial function and left ventricular function compared between the two groups before and after treatment. Results: There were no significant differences in the levels of SBP, DBP, hs-CRP, TNF-α, NO, ET-1, LVEF, LVEDD and LVESD in the two groups before treatment. After treatment, two groups of SBP, DBP, hs-CRP, TNF-α, ET-1, LVEDD and LVESD levels were significantly lower than those in the same group before treatment, and after treatment, the levels of SBP, DBP, hs-CRP, TNF-α, ET-1 and LVESD in the observation group were significantly lower than those in the control group, while there were no significant difference in the level of LVEDD between the two groups after treatment;Compared with level in the group before treatment, the levels of NO and LVEF in the two groups were significantly increased, and the observation group [(82.13±19.01) μmol/L, (52.83±7.45)%] was significantly higher than the control group ((67.37±13.08) μmol/L, (49.47±6.96)%)Conclusion: Telmisartan combined with Nifedipine Controlled Release Table in treating coronary heart disease with mild to moderate hypertension, can effectively control blood pressure, reduce the inflammatory stress, improve vascular endothelial function and left ventricular function of patients, has an important clinical value.
文摘Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases, having one or more than two branches narrow ( ≥70% ) , were diagnosed by coronary angiography. These patients were divided randomly into carvedilol group (n = 28) and control group (n = 23) who did not take carvedilol.Endothelin (ET) and nitro dioxide (NO) levels of peripheral blood were measured before and after PTCA,before and after two weeks by taking carvedilol. Resuits Compared with the ET and NO levels before PTCA, ET were markedly increased and NO were decreased after PTCA (p <0.05 ) ; compared with the ET and NO levels before taking carvedilol, ET were decreased and NO were increased after two week (p <0.05) , but the ET and NO levels of the control group did not change in the period of two weeks observation(p > 0.05). Conclusions Carvedilol may improve the coronary vascular endothelial function after PTCA.
基金Supported by General Program of National Natural Science of China:Qigui Slimming Prescription Upgrades the Activating Ability of the Phlegm-Dampness Constitution to Improve Obesity by Regulating the Thermogenesis Axis of Clostridium Enterica-PhytosphingosineSph K/S1P-Ca^(2+) Cycle(No.82374308)National Key Research and Development Program:Development of Dietary Intervention Series for the Elderly with Decreased Functionality(No.2022YFC2010104)National Nonprofit Institute Research Grant for Institute of Basic Theory for Chinese Medicine,CACMS:Methylation of Gut Microbiota-Host DNA Reveals the Mechanism of Promoting PhlegmDampness Constitution to Prevent Metabolic Diseases(No.YZ-202151)。
文摘OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese volunteers with the phlegmdampness constitution or balanced constitution were recruited for this study respectively.The clinical baseline data was collected,and the vascular endothelial function was evaluated using the EndoPAT?.Blood samples were collected for the serum proteome analysis.The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis,correlation analysis,receiver operating characteristic(ROC)curve analysis,and random forest model investigation were conducted.RESULTS:There were no statistical differences found in the baseline data.For vascular endothelial function,the reactive hyperemia index(RHI)of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group(1.46±0.30 vs 2.82±0.78,P<0.0001),indicating vascular endothelial dysfunction.There are 66 differentially expressed serum proteins between the two groups.apolipoprotein A2(Apo A2),angiotensin-converting enzyme 2(ACE-2),interleukin-33(IL-33),and forkhead box P3(FoxP3)showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.CONCLUSION:Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group.Thus,alterations in the expression levels of key serum proteins,including Apo A2,ACE-2,IL-33,and Fox P3 could serve as potential biomarkers in the obese population at risk of CVD.
基金The study was approved by the Ethics Committee of the Second People's Hospital of Chengdu.
文摘BACKGROUND The incidence and mortality of colorectal cancer(CRC)are among the highest in the world,and its occurrence and development are closely related to tumor neovascularization.When the balance between pigment epithelium-derived factors(PEDF)that inhibit angiogenesis and vascular endothelial growth factors(VEGF)that stimulate angiogenesis is broken,angiogenesis is out of control,resulting in tumor development.Therefore,it is very necessary to find more therapeutic targets for CRC for early intervention and later treatment.AIM To investigate the expression and significance of PEDF,VEGF,and CD31-stained microvessel density values(CD31-MVD)in normal colorectal mucosa,adenoma,and CRC.METHODS In this case-control study,we collected archived wax blocks of specimens from the Digestive Endoscopy Center and the General Surgery Department of Chengdu Second People's Hospital from April 2022 to October 2022.Fifty cases of specimen wax blocks were selected as normal intestinal mucosa confirmed by electronic colonoscopy and concurrent biopsy(normal control group),50 cases of specimen wax blocks were selected as colorectal adenoma confirmed by electronic colonoscopy and pathological biopsy(adenoma group),and 50 cases of specimen wax blocks were selected as CRC confirmed by postoperative pathological biopsy after inpatient operation of general surgery(CRC group).An immunohistochemical staining experiment was carried out to detect PEDF and VEGF expression in three groups of specimens,analyze their differences,study the relationship between the two and clinicopathological factors in CRC group,record CD31-MVD in the three groups,and analyze the correlation of PEDF,VEGF,and CD31-MVD in the colorectal adenoma group and the CRC group.The F test or adjusted F test is used to analyze measurement data statistically.Kruskal-Wallis rank sum test was used between groups for ranked data.The chi-square test,adjusted chi-square test,or Fisher's exact test were used to compare the rates between groups.All differences between groups were compared using the Bonferroni method for multiple comparisons.Spearman correlation analysis was used to test the correlation of the data.The test level(α)was 0.05,and a two-sided P<0.05 was considered statistically significant.RESULTS The positive expression rate and expression intensity of PEDF were gradually decreased in the normal control group,adenoma group,and CRC group(100%vs 78%vs 50%,χ^(2)=34.430,P<0.001;++~++vs+~++vs-~+,H=94.059,P<0.001),while VEGF increased gradually(0%vs 68%vs 96%,χ^(2)=98.35,P<0.001;-vs-~+vs++~+++,H=107.734,P<0.001).In the CRC group,the positive expression rate of PEDF decreased with the increase of differen-tiation degree,invasion depth,lymph node metastasis,distant metastasis,and TNM stage(χ^(2)=20.513,4.160,5.128,6.349,5.128,P<0.05);the high expression rate of VEGF was the opposite(χ^(2)=10.317,13.134,17.643,21.844,17.643,P<0.05).In the colorectal adenoma group,the expression intensity of PEDF correlated negatively with CD31-MVD(r=-0.601,P<0.001),whereas VEGF was not significantly different(r=0.258,P=0.07).In the CRC group,the expression intensity of PEDF correlated negatively with the expression intensity of CD31-MVD and VEGF(r=-0.297,P<0.05;r=-0.548,P<0.05),while VEGF expression intensity was positively related to CD31-MVD(r=0.421,P=0.002).CONCLUSION It is possible that PEDF can be used as a new treatment and prevention target for CRC by upregulating the expression of PEDF while inhibiting the expression of VEGF.
基金supported by Science and Technology Research Project of Jilin Provincial Department of Education,No.JJKH20220072KJ(to XL)Science and Technology Development Program of Jilin Province,No.20200201495JC(to YL)。
文摘The integrity of retinal ganglion cells is tightly associated with diabetic macular degeneration that leads to damage and death of retinal ganglion cells,affecting vision.The major clinical treatments for diabetic macular edema are anti-vascular endothelial growth factor drugs and laser photocoagulation.However,although the macular thickness can be normalized with each of these two therapies used alone,the vision does not improve in many patients.This might result from the incomplete recovery of retinal ganglion cell injury.Therefore,a prospective,non-randomized,controlled clinical trial was designed to investigate the effect of anti-vascular endothelial growth factor drugs combined with laser photocoagulation on the integrity of retinal ganglion cells in patients with diabetic macular edema and its relationship with vision recovery.In this trial,150 patients with diabetic macular edema will be equally divided into three groups according to therapeutic methods,followed by treatment with anti-vascular endothelial growth factor drugs,laser photocoagulation therapy,and their combination.All patients will be followed up for 12 months.The primary outcome measure is retinal ganglion cell-inner plexiform layer thickness at 12 months after treatment.The secondary outcome measures include retinal ganglion cell-inner plexiform layer thickness before and 1,3,6,and 9 months after treatment,retinal nerve fiber layer thickness,best-corrected visual acuity,macular area thickness,and choroidal thickness before and 1,3,6,9,and 12 months after treatment.Safety measure is the incidence of adverse events at 1,3,6,9,and 12 months after treatment.The study protocol hopes to validate the better efficacy and safety of the combined treatment in patients with diabetic macula compared with the other two monotherapies alone during the 12-month follow-up period.The trial is designed to focus on clarifying the time-effect relationship between imaging measures related to the integrity of retinal ganglion cells and best-corrected visual acuity.The trial protocol was approved by the Medical Ethics Committee of the Affiliated Hospital of Beihua University with approval No.(2023)(26)on April 25,2023,and was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR2300072478,June 14,2023,protocol version:2.0).
