Objective： To estimate the relationship between genetic polymorphisms of X-ray repair cross- complementing group 1 （XRCC1） and the susceptibility to childhood acute lymphoblastic leukemia （ALL）. Methods： Relevan...Objective： To estimate the relationship between genetic polymorphisms of X-ray repair cross- complementing group 1 （XRCC1） and the susceptibility to childhood acute lymphoblastic leukemia （ALL）. Methods： Relevant case-control studies were enrolled in the meta-analysis. We applied Rev Man 4.2 software to pool raw data and test studies＇ heterogeneity and to calculate the incorporated odds ratio （OR） and 95% confidence interval （95% CI）. Results： Our data showed that the OR for the Gln allele of the Arg399Gln polymorphism, compared with the Arg allele, was 1.35 （95% CI, 1.16-1.57; P〈0.0001） for childhood ALL patients. Similarly, the homozygous genotype Gln/Gln and heterozygous genotype Arg/Gln both significantly increased the risk of childhood ALL compared with the wild genotype Arg/Arg （OR =1.58; 95% CI, 1.13-2.21; P=0.008; OR =1.51; 95% CI, 1.21-1.87; P=0.0002）. The dominant model of Arg399Gln was associated with childhood ALL risk （OR =1.54; 95% CI, 1.25-1.89; P〈0.0001）. The ethnic subgroup analysis demonstrated that the Gln allele in all five ethnic groups was prone to be a risk factor for childhood ALL just with different degrees of correlation while Arg194Trp SNP showed a protective or risk factor or irrelevant thing in different races. Conclusions： XRCC1 399 polymorphism may increase the risk of childhood ALL. Different ethnic groups with some gene polymorphism have different disease risks.展开更多
Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with a...Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with advanced esophageal cancer who accepted PF chemotherapy in our hospital between May 2013 and October 2015 were selected, esophageal cancer tissue was collected before the chemotherapy, the patients were divided into chemotherapy sensitivity group and chemotherapy resistance group according to the effect of chemotherapy, and the expression levels of ERCC1, GSTP1 as well as apoptosis and proliferation genes in esophageal cancer tissue were detected.Results:Protein content and positive protein expression rate of ERCC1 and GSTP1 in esophageal cancer tissue of chemotherapy sensitivity group were significantly lower than those of chemotherapy resistance group, MBP1, DEC1 and PTEN protein content were significantly higher than those of chemotherapy resistance group, and PLCE1, CyclinD1 and PAR2 protein content were significantly lower than those of chemotherapy resistance group;MBP1, DEC1 and PTEN protein content in esophageal cancer tissue with positive ERCC1 and GSTP1 expression were significantly lower than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression while PLCE1, CyclinD1 and PAR2 protein content were significantly higher than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression.Conclusion:The highly expressed ERCC1 and GSTP1 in esophageal cancer tissue can decreased the cancer cell sensitivity to platinum-based chemotherapeutics, inhibit cell apoptosis and promote cell proliferation during platinum-based chemotherapy.展开更多
文摘Objective： To estimate the relationship between genetic polymorphisms of X-ray repair cross- complementing group 1 （XRCC1） and the susceptibility to childhood acute lymphoblastic leukemia （ALL）. Methods： Relevant case-control studies were enrolled in the meta-analysis. We applied Rev Man 4.2 software to pool raw data and test studies＇ heterogeneity and to calculate the incorporated odds ratio （OR） and 95% confidence interval （95% CI）. Results： Our data showed that the OR for the Gln allele of the Arg399Gln polymorphism, compared with the Arg allele, was 1.35 （95% CI, 1.16-1.57; P〈0.0001） for childhood ALL patients. Similarly, the homozygous genotype Gln/Gln and heterozygous genotype Arg/Gln both significantly increased the risk of childhood ALL compared with the wild genotype Arg/Arg （OR =1.58; 95% CI, 1.13-2.21; P=0.008; OR =1.51; 95% CI, 1.21-1.87; P=0.0002）. The dominant model of Arg399Gln was associated with childhood ALL risk （OR =1.54; 95% CI, 1.25-1.89; P〈0.0001）. The ethnic subgroup analysis demonstrated that the Gln allele in all five ethnic groups was prone to be a risk factor for childhood ALL just with different degrees of correlation while Arg194Trp SNP showed a protective or risk factor or irrelevant thing in different races. Conclusions： XRCC1 399 polymorphism may increase the risk of childhood ALL. Different ethnic groups with some gene polymorphism have different disease risks.
文摘Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with advanced esophageal cancer who accepted PF chemotherapy in our hospital between May 2013 and October 2015 were selected, esophageal cancer tissue was collected before the chemotherapy, the patients were divided into chemotherapy sensitivity group and chemotherapy resistance group according to the effect of chemotherapy, and the expression levels of ERCC1, GSTP1 as well as apoptosis and proliferation genes in esophageal cancer tissue were detected.Results:Protein content and positive protein expression rate of ERCC1 and GSTP1 in esophageal cancer tissue of chemotherapy sensitivity group were significantly lower than those of chemotherapy resistance group, MBP1, DEC1 and PTEN protein content were significantly higher than those of chemotherapy resistance group, and PLCE1, CyclinD1 and PAR2 protein content were significantly lower than those of chemotherapy resistance group;MBP1, DEC1 and PTEN protein content in esophageal cancer tissue with positive ERCC1 and GSTP1 expression were significantly lower than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression while PLCE1, CyclinD1 and PAR2 protein content were significantly higher than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression.Conclusion:The highly expressed ERCC1 and GSTP1 in esophageal cancer tissue can decreased the cancer cell sensitivity to platinum-based chemotherapeutics, inhibit cell apoptosis and promote cell proliferation during platinum-based chemotherapy.
