Objective:The aim of this study was to discover the potential biomarkers changes on a rat model of isoproterenol (ISO) induced myocardial ischaemia before and after treatment with a Xiangdan submicron emulsion and to ...Objective:The aim of this study was to discover the potential biomarkers changes on a rat model of isoproterenol (ISO) induced myocardial ischaemia before and after treatment with a Xiangdan submicron emulsion and to explore the unclearing mechanisms by urinary metabolomics.Methods:Three active traditional Chinese medicine (TCM) ingredients were formulated into a lyophilized submicron emulsion powder with hydroxypropyl-β-cyclodextrin encapsulation by high pressure homogenization technology.The three ingredients were given both separately and combined,and the corresponding effects of the three drugs were compared.Principal component analysis (PCA) and the HPLC-UV analytical method was applied to explore the change of composition of urine metabolites in a rat model of isoproterenol induced myocardial ischaemia.Results:Analytical methods measuring the three active ingredients in urine samples were established.PCA score plots revealed that the therapeutic effect of the Xiangdan submicron emulsion was more significant than that of the Xiangdan injection.There was a synergistic effect among the three active ingredients.Furthermore,studies of rat metabolism changes and structure analysis of biological markers indicated that the mechanism of Xiangdan formulations was related to the inhibition of abnormal tryptophan metabolism.Conclusion:Xiangdan preparations had a confirmed effect in the treatment of coronary heart disease,especially with the novel Xiangdan submicron emulsion formulation.Urinary metabolomics methods to monitor special biomarker changes is an excellent method to reveal the mechanism of action of TCM drugs.展开更多
目的系统评价香丹注射液治疗冠状动脉粥样硬化性心脏病(以下简称“冠心病”)心绞痛的效果和安全性,为临床安全用药提供参考。方法计算机检索中国生物医学文献数据库、中国知网、维普网、PubMed等中英文数据库。检索时限从各数据库建库...目的系统评价香丹注射液治疗冠状动脉粥样硬化性心脏病(以下简称“冠心病”)心绞痛的效果和安全性,为临床安全用药提供参考。方法计算机检索中国生物医学文献数据库、中国知网、维普网、PubMed等中英文数据库。检索时限从各数据库建库之日起到2022年10月24日止。筛选文献,提取资料后使用Cochrane官方网站推荐的偏倚风险评估工具对纳入的文献进行有效评价后,采用Rev Man 5.4软件进行Meta分析。结果最终纳入10项随机对照试验(RCT),总样本量920例。Meta分析结果显示,试验组患者心绞痛疗效、心电图疗效、治疗后低密度脂蛋白胆固醇水平、治疗后血浆黏稠度水平与对照组比较差异均有统计学意义(P<0.05);两组药物不良反应(ADR)发生率差异无统计学意义。结论香丹注射液可用于治疗冠心病心绞痛,安全性较好且对血脂水平、血液黏稠度有一定的改善。其疗效需要进一步扩大样本量加以证实。展开更多
文摘Objective:The aim of this study was to discover the potential biomarkers changes on a rat model of isoproterenol (ISO) induced myocardial ischaemia before and after treatment with a Xiangdan submicron emulsion and to explore the unclearing mechanisms by urinary metabolomics.Methods:Three active traditional Chinese medicine (TCM) ingredients were formulated into a lyophilized submicron emulsion powder with hydroxypropyl-β-cyclodextrin encapsulation by high pressure homogenization technology.The three ingredients were given both separately and combined,and the corresponding effects of the three drugs were compared.Principal component analysis (PCA) and the HPLC-UV analytical method was applied to explore the change of composition of urine metabolites in a rat model of isoproterenol induced myocardial ischaemia.Results:Analytical methods measuring the three active ingredients in urine samples were established.PCA score plots revealed that the therapeutic effect of the Xiangdan submicron emulsion was more significant than that of the Xiangdan injection.There was a synergistic effect among the three active ingredients.Furthermore,studies of rat metabolism changes and structure analysis of biological markers indicated that the mechanism of Xiangdan formulations was related to the inhibition of abnormal tryptophan metabolism.Conclusion:Xiangdan preparations had a confirmed effect in the treatment of coronary heart disease,especially with the novel Xiangdan submicron emulsion formulation.Urinary metabolomics methods to monitor special biomarker changes is an excellent method to reveal the mechanism of action of TCM drugs.
文摘目的系统评价香丹注射液治疗冠状动脉粥样硬化性心脏病(以下简称“冠心病”)心绞痛的效果和安全性,为临床安全用药提供参考。方法计算机检索中国生物医学文献数据库、中国知网、维普网、PubMed等中英文数据库。检索时限从各数据库建库之日起到2022年10月24日止。筛选文献,提取资料后使用Cochrane官方网站推荐的偏倚风险评估工具对纳入的文献进行有效评价后,采用Rev Man 5.4软件进行Meta分析。结果最终纳入10项随机对照试验(RCT),总样本量920例。Meta分析结果显示,试验组患者心绞痛疗效、心电图疗效、治疗后低密度脂蛋白胆固醇水平、治疗后血浆黏稠度水平与对照组比较差异均有统计学意义(P<0.05);两组药物不良反应(ADR)发生率差异无统计学意义。结论香丹注射液可用于治疗冠心病心绞痛,安全性较好且对血脂水平、血液黏稠度有一定的改善。其疗效需要进一步扩大样本量加以证实。
