OBJECTIVE To investigate the cerebralvasorelaxant material basis of Xiaoxuming decoction.METHODS According to the Xiaoxuming decoction herb sources,we retrieved the chemical structure from the literatures and the Chin...OBJECTIVE To investigate the cerebralvasorelaxant material basis of Xiaoxuming decoction.METHODS According to the Xiaoxuming decoction herb sources,we retrieved the chemical structure from the literatures and the Chinese Natural Product Database(http://pharmdata.ncmi.cn).By using microvessel tension system,we checked the vasorelaxanteffects of Xiaoxuming decoction anti-cerebral ischemia effective components group(XXMDECG)and the available composition compounds on pre-contracted basilar artery ring.RESULTS963 compoundsin the decoction,including 81Fangfeng,77 Mahuang,130 Shengjiang,31 Guizhi,91 Huangqin,127 Renshen,73 Chuanxiong,44 Shaoyao,39 Xingren,42 Fangji,62 Fuzi and 166 Gancao were collected.The five largest number classes of compounds in the decoction are volatile oil(32%),flavone(32%),alkaloid(13%),saponin(7%),polyphenol and organic acid(5%).XXMDECG at concentration from 1 to 400μg·mL-1can dilate the KCl(60 mmol·L-1)and ET-1(0.01μmol·L-1)pre-contracted rat basilar artery rings in a dose-dependent manner.There are 6 compounds with vasorelaxant ratio more than 50%at the concentration of 10μmol·L-1.CONCLUSION Xiaoxuming decoction contains abundant chemical structure.It has the material basis of multiple ingredients and multiple targets.The XXMDECG are able to dilate the rat basilar artery rings in a dose-dependent manner.The network interactions between varies of chemical compounds in Xiaoxuming decoction and the vasoconstriction associated targets result in the comprehensive regulation mechanisms of vascular function.展开更多
Objective:To evaluate the clinical efficacy and safety of Xiaoxuming decoction(XXMD)in the treatment of stroke.Methods:Randomized controlled trials of XXMD in the treatment of stroke was searched from January 1,2015 t...Objective:To evaluate the clinical efficacy and safety of Xiaoxuming decoction(XXMD)in the treatment of stroke.Methods:Randomized controlled trials of XXMD in the treatment of stroke was searched from January 1,2015 to March 1,2020.The data extraction and methodological quality evaluation were carried out by two researchers according to the pre-established inclusion and exclusion criteria,and the final inclusion study was analyzed by meta using Revman5.3.Results:22 RCT literatures were included,with a total of 1947 cases.Compared with the control group,the experimental group could reduce the score of NIHSS scale(95%CI(-3.37,-2.19),P<0.001),stroke score(95%CI(-2.71,-1.85),P<0.001),and BI(95%CI(6.15,8.50,P<0.001).And the effective rate of the experimental group was 3.58 times higher than that of the control group,and the difference was statistically significant(95%CI(2.40,5.36),P=0.00001).Conclusion:XXMD combined with western medicine is superior to western medicine in improving the symptoms of neurological deficit and living ability and the effective rate of stroke.In terms of clinical effective rate,the effective rate of XXMD in acute stage is 1.02 times higher than that in non-acute stage.However,no improvement in efficiency was found after syndrome differentiation.Future research may achieve higher efficacy in syndrome types other than Fengtanyuzu.展开更多
OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into ...OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into eight groups(n=12),including blank group,model group,medication group,acupuncture group,high-dose XXMD group(XXMD-H),low-dose XXMD group(XXMD-L),acupuncture combined with high-dose XXMD group(A+H),and acupuncture combined with low-dose XXMD group(A+L).After treatment for 6 weeks,dopamine(DA)neurons and the pathological changes of tyrosine hydroxylase(TH)positive cells were observed.The enzyme-linked immunosorbent assay(ELISA)was used to measure the content of DA and the level of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor alpha(TNF-α).The m RNA level of PINK1 and Parkin and the protein expression of Nix,PINK1 and Parkin in the substantia nigra were also detected.RESULTS:Combination treatment effectively ameliorated the symptoms of PD.Compared with model group,combined treatment significantly up-regulated the protein expression of Nix,Parkin and PINK1 and the m RNA levels of PINK1 and Parkin in the substantia nigra(P<0.0001,P<0.001,P<0.01 or P<0.05).Furthermore,the levels of pro-inflammation cytokines were obviously decreased after combination therapy,while IL-10 content was increased remarkably(P<0.01).CONCLUSION:Compared with each treatment alone,combination therapy improved the pathological damage of DA neurons of PD mice more effectively.The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function.These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.展开更多
目的 基于质量源于设计(quality by design,QbD)理念,建立小续命汤提取液喷雾干燥工艺。方法 采用Plackeet-Burmann设计从药液相对密度、进料速度、进风温度、药液温度和雾化压力中筛选关键工艺参数(Critical Process Parameter,CPP),...目的 基于质量源于设计(quality by design,QbD)理念,建立小续命汤提取液喷雾干燥工艺。方法 采用Plackeet-Burmann设计从药液相对密度、进料速度、进风温度、药液温度和雾化压力中筛选关键工艺参数(Critical Process Parameter,CPP),运用中心点复合设计实验对筛选得到的CPP进行优化,建立小续命汤提取液喷雾干燥工艺设计空间,同时建立物理指纹图谱并进行相似度评价。结果 药液相对密度和药液温度为关键工艺参数,CPP优化后构建的设计空间为药液相对密度1.07~1.10,药液温度30~33℃,5批验证试验的喷干粉物理指纹图谱相似度均大于0.99。结论 基于QbD理念建立的小续命汤提取液喷雾干燥工艺稳定可行,可为小续命汤颗粒中试放大研究提供参考。展开更多
Aim:To analyze petroleum ether extracts of Xiaoxuming decoction and develop the methods for the quality control of the extract.Method:A method based on GC was developed.In qualitative analysis,samples were analyzed be...Aim:To analyze petroleum ether extracts of Xiaoxuming decoction and develop the methods for the quality control of the extract.Method:A method based on GC was developed.In qualitative analysis,samples were analyzed before and after derivation.In quantitative analysis,MS and FID detectors were used.Result:Cinnamic aldehyde was detected without derivation.And three kinds of fatty acids existing in almond oil were detected after derivation.Under the selected condition,cinnamic aldehyde was separated and detected.MS and FID detection generated the recoveries of 104.2% and 103.8% of cinnamic aldehyde with RSDs of 2.2% and 2.8%,respectively.Conclusion:This method is simple and reproducible,and it can be used for the quality control of petroleum ether extracts of Xiaoxuming decoction.展开更多
基金The project supported by Major Scientific and Technological Special Project for "Significant New Drug Creation"(2013ZX09508104,2013ZX09402203)by Central Public Scientific Research Institution Fundamental Project(2014CX05)
文摘OBJECTIVE To investigate the cerebralvasorelaxant material basis of Xiaoxuming decoction.METHODS According to the Xiaoxuming decoction herb sources,we retrieved the chemical structure from the literatures and the Chinese Natural Product Database(http://pharmdata.ncmi.cn).By using microvessel tension system,we checked the vasorelaxanteffects of Xiaoxuming decoction anti-cerebral ischemia effective components group(XXMDECG)and the available composition compounds on pre-contracted basilar artery ring.RESULTS963 compoundsin the decoction,including 81Fangfeng,77 Mahuang,130 Shengjiang,31 Guizhi,91 Huangqin,127 Renshen,73 Chuanxiong,44 Shaoyao,39 Xingren,42 Fangji,62 Fuzi and 166 Gancao were collected.The five largest number classes of compounds in the decoction are volatile oil(32%),flavone(32%),alkaloid(13%),saponin(7%),polyphenol and organic acid(5%).XXMDECG at concentration from 1 to 400μg·mL-1can dilate the KCl(60 mmol·L-1)and ET-1(0.01μmol·L-1)pre-contracted rat basilar artery rings in a dose-dependent manner.There are 6 compounds with vasorelaxant ratio more than 50%at the concentration of 10μmol·L-1.CONCLUSION Xiaoxuming decoction contains abundant chemical structure.It has the material basis of multiple ingredients and multiple targets.The XXMDECG are able to dilate the rat basilar artery rings in a dose-dependent manner.The network interactions between varies of chemical compounds in Xiaoxuming decoction and the vasoconstriction associated targets result in the comprehensive regulation mechanisms of vascular function.
文摘Objective:To evaluate the clinical efficacy and safety of Xiaoxuming decoction(XXMD)in the treatment of stroke.Methods:Randomized controlled trials of XXMD in the treatment of stroke was searched from January 1,2015 to March 1,2020.The data extraction and methodological quality evaluation were carried out by two researchers according to the pre-established inclusion and exclusion criteria,and the final inclusion study was analyzed by meta using Revman5.3.Results:22 RCT literatures were included,with a total of 1947 cases.Compared with the control group,the experimental group could reduce the score of NIHSS scale(95%CI(-3.37,-2.19),P<0.001),stroke score(95%CI(-2.71,-1.85),P<0.001),and BI(95%CI(6.15,8.50,P<0.001).And the effective rate of the experimental group was 3.58 times higher than that of the control group,and the difference was statistically significant(95%CI(2.40,5.36),P=0.00001).Conclusion:XXMD combined with western medicine is superior to western medicine in improving the symptoms of neurological deficit and living ability and the effective rate of stroke.In terms of clinical effective rate,the effective rate of XXMD in acute stage is 1.02 times higher than that in non-acute stage.However,no improvement in efficiency was found after syndrome differentiation.Future research may achieve higher efficacy in syndrome types other than Fengtanyuzu.
基金Supported by National Natural Science Foundation of China:Study on the Mechanism of Regulating miR-124 by Tongdu Tiaoshen Acupuncture to Promote Neuroprotection in Cerebral Ischemia Reperfusion Injury(No.81973933)Natural Science Fund for Colleges and Universities in Anhui Province:Study on the Regulatory Mechanism of Tongdu Tiaoshen Acupuncture on Dopaminergic Neurons in Parkinson’s Disease Model Mice Based on Mitochondrial Autophagy(No.KJ2019A0475)。
文摘OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into eight groups(n=12),including blank group,model group,medication group,acupuncture group,high-dose XXMD group(XXMD-H),low-dose XXMD group(XXMD-L),acupuncture combined with high-dose XXMD group(A+H),and acupuncture combined with low-dose XXMD group(A+L).After treatment for 6 weeks,dopamine(DA)neurons and the pathological changes of tyrosine hydroxylase(TH)positive cells were observed.The enzyme-linked immunosorbent assay(ELISA)was used to measure the content of DA and the level of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor alpha(TNF-α).The m RNA level of PINK1 and Parkin and the protein expression of Nix,PINK1 and Parkin in the substantia nigra were also detected.RESULTS:Combination treatment effectively ameliorated the symptoms of PD.Compared with model group,combined treatment significantly up-regulated the protein expression of Nix,Parkin and PINK1 and the m RNA levels of PINK1 and Parkin in the substantia nigra(P<0.0001,P<0.001,P<0.01 or P<0.05).Furthermore,the levels of pro-inflammation cytokines were obviously decreased after combination therapy,while IL-10 content was increased remarkably(P<0.01).CONCLUSION:Compared with each treatment alone,combination therapy improved the pathological damage of DA neurons of PD mice more effectively.The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function.These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.
文摘目的 基于质量源于设计(quality by design,QbD)理念,建立小续命汤提取液喷雾干燥工艺。方法 采用Plackeet-Burmann设计从药液相对密度、进料速度、进风温度、药液温度和雾化压力中筛选关键工艺参数(Critical Process Parameter,CPP),运用中心点复合设计实验对筛选得到的CPP进行优化,建立小续命汤提取液喷雾干燥工艺设计空间,同时建立物理指纹图谱并进行相似度评价。结果 药液相对密度和药液温度为关键工艺参数,CPP优化后构建的设计空间为药液相对密度1.07~1.10,药液温度30~33℃,5批验证试验的喷干粉物理指纹图谱相似度均大于0.99。结论 基于QbD理念建立的小续命汤提取液喷雾干燥工艺稳定可行,可为小续命汤颗粒中试放大研究提供参考。
文摘Aim:To analyze petroleum ether extracts of Xiaoxuming decoction and develop the methods for the quality control of the extract.Method:A method based on GC was developed.In qualitative analysis,samples were analyzed before and after derivation.In quantitative analysis,MS and FID detectors were used.Result:Cinnamic aldehyde was detected without derivation.And three kinds of fatty acids existing in almond oil were detected after derivation.Under the selected condition,cinnamic aldehyde was separated and detected.MS and FID detection generated the recoveries of 104.2% and 103.8% of cinnamic aldehyde with RSDs of 2.2% and 2.8%,respectively.Conclusion:This method is simple and reproducible,and it can be used for the quality control of petroleum ether extracts of Xiaoxuming decoction.
