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Detection and Identification of Phytoplasma of Cleome rutidosperma in Areca Palm Yellow Leaf Disease Field
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作者 Zhaowei LIN Xiaoqing NIU +3 位作者 Shida LONG Qinghua TANG Dejie YANG Weiwei SONG 《Plant Diseases and Pests》 2024年第4期7-12,共6页
[Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed... [Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed to amplify the phytoplasma 16S rDNA of C.rutidosperma samples,followed by sequence analysis.Concurrently,this study examined C.rutidosperma in YLD field,collecting symptomatic leaves for phytoplasma detection.[Results]The 16S rDNA sequence of the C.rutidosperma witches'-broom phytoplasma was found to be identical to that of the HNWC5 strain associated with areca palm yellows phytoplasma,leading to the identification of this phytoplasma as belonging to the 16SrII-A subgroup.Field investigations revealed a higher incidence of C.rutidosperma in areca palm fields,with symptoms of leaf yellows observed in six of these fields.Quantitative PCR(qPCR)analysis confirmed the presence of phytoplasma infection in these instances.[Conclusions]Through the analysis of geographical distribution,sequence alignment,and field occurrence data,a significant correlation has been identified between witches'broom disease and YLD.It is proposed that the former may act as an intermediate host for the areca palm yellows phytoplasma. 展开更多
关键词 Areca palm yellow leaf disease PHYTOPLASMA Cleome rutidosperma IDENTIFICATION Detection
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A Review of Study of Yellow Water Disease in Tibetan Medicine Theory
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作者 Han XIAO Lili HE 《Medicinal Plant》 CAS 2023年第5期106-109,共4页
Based on the Tibetan medical theory,the relevant information and diagnosis and treatment ideas of yellow water disease are discussed,and Mongolian medicine also takes its own basic medical theory as the starting point... Based on the Tibetan medical theory,the relevant information and diagnosis and treatment ideas of yellow water disease are discussed,and Mongolian medicine also takes its own basic medical theory as the starting point to discuss and explain,while traditional Chinese medicine has made less theoretical description of this disease,but there are also some understandings and treatment guidelines.This paper mainly discusses the cognitive aspects of this disease,starting from the essence,analyzes the relationship between this disease and traditional Chinese medicine diseases as well as modern medicine,and makes a theoretical description for a better understanding of the yellow water disease. 展开更多
关键词 Yellow water disease Tibetan medicine Traditional Chinese medicine disease Modern medicine
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Screening of Growth-Promoting Strains for Areca Palm
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作者 Dejie YANG Yenan WANG +1 位作者 Zhaowei LIN Xiaoqing NIU 《Plant Diseases and Pests》 2024年第5期1-4,共4页
[Objectives]The paper was to identify growth-promoting strains within the culturable bacterial flora of areca palm.[Methods]Culturable bacteria were isolated and identified from areca palm using samples obtained from ... [Objectives]The paper was to identify growth-promoting strains within the culturable bacterial flora of areca palm.[Methods]Culturable bacteria were isolated and identified from areca palm using samples obtained from both healthy and yellowing disease-affected plants within the same orchard.Strains that exhibited significant differences between healthy and affected samples,or that were unique to the healthy samples,were subsequently screened for their growth-promoting effects.[Results]Three bacterial strains demonstrated robust and consistent capacity for auxin production,specifically Paenibacillus,Pseudomonas aeruginosa,and Bacillus amyloliquefaciens,each yielding approximately 50μg of IAA per mL of bacterial solution.The strain Alcaligenes faecalis exhibited the highest efficacy in siderophore production,achieving 21.15%of active units.Additionally,A.faecalis,Bacillus velezensis,and P.aeruginosa were noted for their potassium-solubilizing capabilities,as evidenced by the presence of distinct potassium-solubilizing zones.[Conclusions]The evaluation of the aforementioned growth-promoting strains may offer valuable insights for the development of growth-promoting strains specifically for areca palm. 展开更多
关键词 Areca palm Yellow leaf disease Auxin production Siderophore production Potassium-solubilizing
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Development of a Liquid Chip Technique to Simultaneously Detect Taura Syndrome Virus( TSV) and Yellow Head Disease Virus( YHDV) 被引量:2
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作者 Yin Weili Zhang Sihua +2 位作者 Yue Zhiqin Zheng Xiaolong Liu Hong 《Animal Husbandry and Feed Science》 CAS 2014年第5期256-260,共5页
The aim is to develop a liquid chip technique to detect Taura syndrome virus( TSV) and yellow head disease virus( YHDV) on Penaeus orientalis simultaneously. The CP2 gene of TSV and N gene of YHDV in Gen Bank was anal... The aim is to develop a liquid chip technique to detect Taura syndrome virus( TSV) and yellow head disease virus( YHDV) on Penaeus orientalis simultaneously. The CP2 gene of TSV and N gene of YHDV in Gen Bank was analysed by using the software DNAStar 7. 0 to design the TSV-and YHDV-specific primers. The primers were labeled with biotin and subjected to amination modification. They were then coupled with fluorescence-coded microspheres and then used for hybridization with RT- PCR products of TSV and YHDV. The liquid chip detection technique for detection of TSV and YHDV was established by using BD FACSArray to detect fluorescence signal in the reaction system. This assay system had a high sensitivity to TSV and YHDV,with the detection of limit of 100 pg. Moreover,the assay was specific for the detection of TSV,YHDV and was not susceptible to cross with other viruses,including white spot syndrome virus( WSSV),spring viremia of carp virus( SVCV),infectious haematopoietic necrosis virus( IHNV). In conclusion,the liquid chip assay technique established in this study is highly sensitive and specific to TSV and YHDV detection. Moreover,it provides a novel,convenient and rapid approach for the detection of TSV and YHDV. 展开更多
关键词 Penaeus orientalis Taura syndrome virus(TSV) Yellow head disease virus(YHDV) Liquid chip Detection
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Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence
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作者 Prasanna H C Kanakala S +3 位作者 Archana K Jyothsna P Varma R K Malathi V G 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第9期1786-1795,共10页
Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving ... Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitelfy species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. Al the haplotypes detected in the study could be assigned to these three cryptic species fol owing the species demarcation criteria of 3.5%divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribu-tion. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a signiifcantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitelfy species in India and yel ow mosaic disease observed during sampling survey. 展开更多
关键词 WHITEFLY mitochondrial cytochrome oxidase gene subunit-1 AsiaⅠ AsiaⅡ 1 AsiaⅡ 7 begomovirus and yellow mosaic disease
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Development of high yield and tomato yellow leaf curl virus(TYLCV)resistance using conventional and molecular approaches:A review
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作者 THARANGANI WELEGAMA MOHD Y.RAFII +2 位作者 KHAIRULMAZMI AHMAD SHAIRUL I.RAMLEE YUSUFF OLADOSU 《BIOCELL》 SCIE 2021年第4期1069-1079,共11页
Tomato(Solanum lycopersicum L.)belonging to the family Solanaceae is the second most consumed and cultivated vegetable globally.Since the ancient time of its domestication,thousands of cultivated tomato varieties have... Tomato(Solanum lycopersicum L.)belonging to the family Solanaceae is the second most consumed and cultivated vegetable globally.Since the ancient time of its domestication,thousands of cultivated tomato varieties have been developed targeting an array of aspects.Among which breeding for yield and yield-related traits are mostly focused.Cultivated tomato is extremely genetically poor and hence it is a victim for several biotic and abiotic stresses.Among the biotic stresses,the impact of viral diseases is critical all over tomato cultivating areas.Improvement of tomato still largely rely on conventional methods worldwide while molecular approaches,particularly Marker Assisted Selection(MAS)has become popular across the globe as a fast,low cost and precise tool which is essential in present day plant breeding.In this review paper,breeding tomato for high yield and viral disease resistance,particularly to tomato yellow leaf curl virus disease(TYLCVD)using conventional and molecular approaches will be discussed.Lining up of this set of information will be useful to those who are interested in tomato variety development with high yielding and TYLCVD resistance. 展开更多
关键词 Molecular markers Tomato yellow leaf curl virus disease Resistance breeding Solanum lycopersicum High yield
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Mapping of a Wheat Resistance Gene to Yellow Mosaic Disease by Amplified Fragment Length Polymorphism and Simple Sequence Repeat Markers 被引量:10
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作者 Wei-Hua LIU Huan NIE +6 位作者 Zhen-Tian HE Xiu-Lan CHEN Yue-Peng HAN Jin-Rong WANG Xin LI Cheng-Gui HAN Jia-Lin YU 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第9期1133-1139,共7页
Wheat (Triticum aestivum L.) yellow mosaic virus (WYMV) is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted pl... Wheat (Triticum aestivum L.) yellow mosaic virus (WYMV) is transmitted by a fungal vector through soil and causes serious wheat yield losses due to yellow mosaic disease, with yellow-streaked leaves and stunted plants. In the present study, the amplified fragment length polymorphisms (AFLP) and simple sequence repeat (SSR) were used to identify the molecular linkages with the resistance gene against WYMV. Bulked segregant analysis was performed with an F2 population derived from the cross of cultivar Ningmai 9 (resistant) × cultivar Yangmai 10 (susceptible). By screening among the resistant or susceptible parents, the F2 pools and the individuals in the F2 population with 64 combined selective AFLP primers (EcoRI/MseI) or 290 reported SSR primers, a polymorphic DNA segment (approximately 120 bp) was amplified using the primer pair E2/M5, and an SSR marker (approximately 180 bp) was located on wheat chromosome 2A using the primer Xgwm328. Analysis with MAPMAKER/Exp Version 3.0b (Whitehead institute for Biomedical Research, Cambridge, MA, USA) indicated that these two markers were dominantly associated with the resistance gene at distances of 5.4 cM or 17.6 cM, respectively. The resistance gene to WYMV derived from Ningmai 9, is temporarily named YmNM, and was mapped to wheat chromosome 2A. 展开更多
关键词 locus on chromosome molecular marker resistance simple sequence repeat WHEAT yellow mosaic disease.
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