In the present study,we aimed to investigate the protective effect of Yuebi Jiazhu Decoction(YBJZD)on the kidney of adriamycin nephropathy(AN)rats.Rats were injected with adriamycin for modeling,except for the control...In the present study,we aimed to investigate the protective effect of Yuebi Jiazhu Decoction(YBJZD)on the kidney of adriamycin nephropathy(AN)rats.Rats were injected with adriamycin for modeling,except for the control group.After the successful establishment of the animal model,rats were randomly divided into the model group,YBJZD low-,medium-,and high-dose groups,and the positive group.The 24-h urine samples were collected.Biochemical indicators were monitored,and kidney tissues were collected for pathological analysis using light microscopy.The results showed that through 4 weeks of drug intervention,the urinary protein level was lower in the YBJZD and positive groups compared with the model group(P<0.05).Serum levels of BUN,SCr,and TG were significantly lower(P<0.01),and ALB was significantly higher(P<0.01)in the YBJZD and positive groups compared with the model group.Compared with the model group,the pathological injury of kidney tissue in the YBJZD and positive groups was significantly alleviated.These outcomes proved that YBJZD had a renal protective effect on AN rats.展开更多
Objective:The objective of the study was to study the potential biological mechanism of Yuebi plus Banxia Decoction(YBD)in the treatment of asthma based on network pharmacology.Materials and Methods:Literature databas...Objective:The objective of the study was to study the potential biological mechanism of Yuebi plus Banxia Decoction(YBD)in the treatment of asthma based on network pharmacology.Materials and Methods:Literature databases were used to collect information on the chemical components and pharmacokinetics of YBD as comprehensively as possible.According to pharmacokinetic information and effective ingredient screening criteria,effective chemical components of YBD were screened,and the target genes corresponding to the effective components were collected by the ligand prediction method.At the same time,literature databases including five disease target gene databases were used to collect asthma disease target genes.Then,the effective component target network of YBD and the asthma disease target network can be constructed using Cytoscape 3.2.1 software.The core targets of YBD for the treatment of asthma were screened according to topological analysis based on degree parameters.Through the analysis of Kyoto Encyclopedia of Genes and Genomes(KEGG)signal pathway,the main mechanisms of YBD in treating asthma were found out.Results:A161 active ingredients of YBD and 136 core targets for the treatment of asthma were screened out.The effective signaling pathways of YBD in the treatment of asthma were mainly focused on ErbB,MAPK,Estrogen,PI3 K-Akt,Neurotrophin,Hippo,HIF-1,TGF-,FoxO,Wnt,Chemoking,Toll-like receptor,vascular endothelial growth factor(VEGF),GnRH,and Notch.Conclusion:YBD has the characteristics of multiple targets and multiple pathways in the treatment of asthma.Its main biological mechanism is related to ErbB,MAPK,Estrogen,PI3 K-Akt,neurotrophin,Hippo,HIF-1,TGF-,FoxO,Wnt,Chemoking,Toll-like receptor,VEGF,GnRH,and Notch.展开更多
基金supported by Applied basic Research Project of Science and Technology Department of Shanxi Province(Grant No.201901D-111343)the Open Project of Key Laboratory of Shanxi Province(Grant No.SXIDL-2018-09)Shanxi University of Traditional Chinese Medicine Science and Technology Innovation Ability Training program“basic Research special project”(Grant No.2020PY-JC-20).
文摘In the present study,we aimed to investigate the protective effect of Yuebi Jiazhu Decoction(YBJZD)on the kidney of adriamycin nephropathy(AN)rats.Rats were injected with adriamycin for modeling,except for the control group.After the successful establishment of the animal model,rats were randomly divided into the model group,YBJZD low-,medium-,and high-dose groups,and the positive group.The 24-h urine samples were collected.Biochemical indicators were monitored,and kidney tissues were collected for pathological analysis using light microscopy.The results showed that through 4 weeks of drug intervention,the urinary protein level was lower in the YBJZD and positive groups compared with the model group(P<0.05).Serum levels of BUN,SCr,and TG were significantly lower(P<0.01),and ALB was significantly higher(P<0.01)in the YBJZD and positive groups compared with the model group.Compared with the model group,the pathological injury of kidney tissue in the YBJZD and positive groups was significantly alleviated.These outcomes proved that YBJZD had a renal protective effect on AN rats.
文摘Objective:The objective of the study was to study the potential biological mechanism of Yuebi plus Banxia Decoction(YBD)in the treatment of asthma based on network pharmacology.Materials and Methods:Literature databases were used to collect information on the chemical components and pharmacokinetics of YBD as comprehensively as possible.According to pharmacokinetic information and effective ingredient screening criteria,effective chemical components of YBD were screened,and the target genes corresponding to the effective components were collected by the ligand prediction method.At the same time,literature databases including five disease target gene databases were used to collect asthma disease target genes.Then,the effective component target network of YBD and the asthma disease target network can be constructed using Cytoscape 3.2.1 software.The core targets of YBD for the treatment of asthma were screened according to topological analysis based on degree parameters.Through the analysis of Kyoto Encyclopedia of Genes and Genomes(KEGG)signal pathway,the main mechanisms of YBD in treating asthma were found out.Results:A161 active ingredients of YBD and 136 core targets for the treatment of asthma were screened out.The effective signaling pathways of YBD in the treatment of asthma were mainly focused on ErbB,MAPK,Estrogen,PI3 K-Akt,Neurotrophin,Hippo,HIF-1,TGF-,FoxO,Wnt,Chemoking,Toll-like receptor,vascular endothelial growth factor(VEGF),GnRH,and Notch.Conclusion:YBD has the characteristics of multiple targets and multiple pathways in the treatment of asthma.Its main biological mechanism is related to ErbB,MAPK,Estrogen,PI3 K-Akt,neurotrophin,Hippo,HIF-1,TGF-,FoxO,Wnt,Chemoking,Toll-like receptor,VEGF,GnRH,and Notch.
