[Objectives]The species and distribution of root-knot nematodes in tobacco growing regions of Yunnan were investigated,in order to provide reference for comprehensive prevention of root-knot nematodes disease and toba...[Objectives]The species and distribution of root-knot nematodes in tobacco growing regions of Yunnan were investigated,in order to provide reference for comprehensive prevention of root-knot nematodes disease and tobacco variety layout. [Methods]Fifty-four samples of root-knot nematode host on tobacco crops were collected from Kunming,Qujing and Yuxi cities in Yunnan from August to October in 2015. The species of root-knot nematodes were identified by observing perineal patterns and molecular biological testing（ PCR of ribosomal DNA of ITS region and sequence characterized amplified region,SCAR）. [Results] There were 24 samples belonging to Meloidogyne arenaria,accounting for 44. 44%; seven M. incognita samples,accounting for 12. 96%; one M. javanica sample,accounting for 1. 85%. Eleven samples were compound populations of M. javanica and M. arenaria,accounting for 20. 37%. Five were compound populations of M. incognita and M. arenaria,accounting for 9. 26%. Three were compound populations of M. incognita and M. javanica,accounting for 5. 56%. Three belonged to compound population of M. incognita,M. javanica and M. arenaria,accounting for 5. 56%. [Conclusions] The species of root-knot nematodes in tobacco growing areas of Yunnan mainly include M. incognita,M. javanica and M. arenaria. M. arenaria is the dominant population.展开更多
[Objective] The research aimed to study clustering of the six climatic factors in Yunnan tobacco planting zone. [Method] 6 meteorological elements in 89 tobacco-growing counties and 12 sub-prefectures were conducted c...[Objective] The research aimed to study clustering of the six climatic factors in Yunnan tobacco planting zone. [Method] 6 meteorological elements in 89 tobacco-growing counties and 12 sub-prefectures were conducted clustering analysis. According to indicator and climate characteristics of the each type, climate in tobacco planting area of Yunnan Province was divided. [Result] Climate in tobacco planting area of Yunnan Province could be divided into eight types: Jiangchuan (24 counties, belonged to northern and central subtropical climate belts), Songming (27 counties, belonged to northern subtropical and central, south, north temperate climate belts), Tengchong (3 counties, belonged to northern subtropical climate belt), Mile (12 counties, belonged to central and southern subtropical climate belts), Qiubei (11 counties, belonged to southern subtropical climate belt), Yanjin (4 counties, belonged to central subtropical humid climate belt), Yuanjiang (4 counties, belonged to southern subtropical and northern tropical climate belts), Zhenxiong (3 counties, belonged to warm temperate and northern subtropical climate belts) were eight representatives. Among 1-8 eco-zones, domestic and foreign cities where climate reached level-one similarity were respectively 3, 1, 1, 0, 1, 1, 0 and 1, up to level-two similarity, respectively 12, 15, 3, 13, 13, 1, 5 and 3. Among 8 major ecological zones, similar distance of the city reaching level-one similarity was in the range of 0.28 to 0.45, and similar degree was the highest. Variety introduction among these places would be successful. Similar distance of the city reaching level-two similarity was between 0.51 and 1.00, and similar degree was higher. Mutual variety introduction had high successful rate in these places. [Conclusion] The research provided theoretical basis for selecting new suitable tobacco variety and optimizing tobacco variety layout in different zones.展开更多
基金Supported by Specialized Research Fund for Doctoral Program of Colleges and Universities of the Ministry of Education(20135302110003)Backup Talent Cultivation Project of Young and Middle-aged Academic Technology Leaders in Yunnan Province(2009CI054)
文摘[Objectives]The species and distribution of root-knot nematodes in tobacco growing regions of Yunnan were investigated,in order to provide reference for comprehensive prevention of root-knot nematodes disease and tobacco variety layout. [Methods]Fifty-four samples of root-knot nematode host on tobacco crops were collected from Kunming,Qujing and Yuxi cities in Yunnan from August to October in 2015. The species of root-knot nematodes were identified by observing perineal patterns and molecular biological testing（ PCR of ribosomal DNA of ITS region and sequence characterized amplified region,SCAR）. [Results] There were 24 samples belonging to Meloidogyne arenaria,accounting for 44. 44%; seven M. incognita samples,accounting for 12. 96%; one M. javanica sample,accounting for 1. 85%. Eleven samples were compound populations of M. javanica and M. arenaria,accounting for 20. 37%. Five were compound populations of M. incognita and M. arenaria,accounting for 9. 26%. Three were compound populations of M. incognita and M. javanica,accounting for 5. 56%. Three belonged to compound population of M. incognita,M. javanica and M. arenaria,accounting for 5. 56%. [Conclusions] The species of root-knot nematodes in tobacco growing areas of Yunnan mainly include M. incognita,M. javanica and M. arenaria. M. arenaria is the dominant population.
基金Supported by Department of Science and Education,State Bureau,China(04A26)
文摘[Objective] The research aimed to study clustering of the six climatic factors in Yunnan tobacco planting zone. [Method] 6 meteorological elements in 89 tobacco-growing counties and 12 sub-prefectures were conducted clustering analysis. According to indicator and climate characteristics of the each type, climate in tobacco planting area of Yunnan Province was divided. [Result] Climate in tobacco planting area of Yunnan Province could be divided into eight types: Jiangchuan (24 counties, belonged to northern and central subtropical climate belts), Songming (27 counties, belonged to northern subtropical and central, south, north temperate climate belts), Tengchong (3 counties, belonged to northern subtropical climate belt), Mile (12 counties, belonged to central and southern subtropical climate belts), Qiubei (11 counties, belonged to southern subtropical climate belt), Yanjin (4 counties, belonged to central subtropical humid climate belt), Yuanjiang (4 counties, belonged to southern subtropical and northern tropical climate belts), Zhenxiong (3 counties, belonged to warm temperate and northern subtropical climate belts) were eight representatives. Among 1-8 eco-zones, domestic and foreign cities where climate reached level-one similarity were respectively 3, 1, 1, 0, 1, 1, 0 and 1, up to level-two similarity, respectively 12, 15, 3, 13, 13, 1, 5 and 3. Among 8 major ecological zones, similar distance of the city reaching level-one similarity was in the range of 0.28 to 0.45, and similar degree was the highest. Variety introduction among these places would be successful. Similar distance of the city reaching level-two similarity was between 0.51 and 1.00, and similar degree was higher. Mutual variety introduction had high successful rate in these places. [Conclusion] The research provided theoretical basis for selecting new suitable tobacco variety and optimizing tobacco variety layout in different zones.
