Banana bunchy top virus Chinese Zhangzhou isolate (BBTV-ZZ) DNA 4 was amplified by PCR and cloned. Sequence analysis showed that BBTV-ZZ DNA 4 is 1 039 nucleotides (nts) in length and this virus could be one member of...Banana bunchy top virus Chinese Zhangzhou isolate (BBTV-ZZ) DNA 4 was amplified by PCR and cloned. Sequence analysis showed that BBTV-ZZ DNA 4 is 1 039 nucleotides (nts) in length and this virus could be one member of BBTV Asian group. Transcriptional initiation site A, which is at the 269 nucleotide, was preliminarily determined by using 5' RACE method. BBTV-ZZ DNA 4 non-coding region was sub-cloned by PCR and inserted into upstream of gfp : : gus plant expression vector pCAMBIA 1304 to construct recombinant plasmid pTA2. Agrobacterium tumefaciens harboring pTA2 was injected into leaves of the tobacco (Nicotiana tabacum L. cv. Xanthi NC) via Agrobacterium-infiltration procedure. Transient expressions of GUS and GFP were determined in injected leaves 3 - 5 d later. GUS activities of pTA2, pCAMBIA 1304 injected and non-injected tobacco leaves respectively were 1.007 0 pmol MU(.)mug(-1.)min(-1), 2.069 0 pmol MU(.)mug(-1.)min(-1) and 0.021 4 pmol MU(.)mug(-1.)min(-1). Indirect ELISA for GFP in 1 mg total protein from pTA2, pCAMBIA 1304 injected and non-injected leaves showed an A(490 nm) value of 89.577, 100.440 and 3.287, respectively. These results showed that the non-coding region of BBTV-ZZ DNA 4 has a promoter activity not only in the virus replication in monocot, but also in driving the expression of a foreign gene in dicot plants.展开更多
From December 3 to 12,2017,Zhangzhou experienced a haze weather process lasting for 10 days.In this paper,based on surface meteorological observation and environmental monitoring data,statistical comparison,Eta correl...From December 3 to 12,2017,Zhangzhou experienced a haze weather process lasting for 10 days.In this paper,based on surface meteorological observation and environmental monitoring data,statistical comparison,Eta correlation coefficient,clustering analysis and other methods were adopted to analyze the causes of haze weather in the whole process from clean air (December 2) to haze generation,maintenance (December 3-12) and dissipation (December 13).The results showed that two blasts of strong cold air invaded the city during the haze formation and maintenance,and regional pollution transport and static and stable weather were the main reasons for the continuous high concentration of particulate matter,the occurrence of mild pollution from PM 10 and PM 2.5 in some periods and mild-heavy haze weather lasting for 10 days.In this process,PM concentration had a strong negative correlation with air temperature and visibility,and the absolute values of the correlation coefficients were between 0.6 and 0.8.PM concentration correlated with relative humidity positively and with wind speed weakly.Regional transport of particulate matter and ozone was conducted in the form of northeast wind and southeast wind backflow.At night and in the morning,under the influence of static and stable weather and long-term sea and land breeze (6 d),average wind speed was less than 1.5 m/s,and the frequency of static wind and small wind exceeded 50%,while there was no precipitation.At 08:00,in the middle and low altitudes,the inversion layer appeared in one week,and cloud cover was large,while the height of mixed layer was below 600 m.In the whole process,it was in the weak divergence field under cold high pressure,and the overall diffusion,dilution and cleaning ability of the atmosphere were poor.展开更多
Taking the construction of Green Lake Eco-park for example, this paper introduced the achievements and experience of this project in rural land expropriation, ecological conservation, humanistic development, and comme...Taking the construction of Green Lake Eco-park for example, this paper introduced the achievements and experience of this project in rural land expropriation, ecological conservation, humanistic development, and commercial development via field investigation, literature consultation and resident interview, and also problems in the current construction. Then suggestions were put forward to provide references for the further improvement of the Green Lake Eco-park.展开更多
Reservoir temperature is an essential parameter in geothermal exploration, which is usually obtained by using chemical geothermometers. There are more than a dozen of conventional geothermometers, each of which has it...Reservoir temperature is an essential parameter in geothermal exploration, which is usually obtained by using chemical geothermometers. There are more than a dozen of conventional geothermometers, each of which has its own limitation for application. Zhangzhou Geothermal Field is a saline water field, the thermal water chemistry of which展开更多
文摘Banana bunchy top virus Chinese Zhangzhou isolate (BBTV-ZZ) DNA 4 was amplified by PCR and cloned. Sequence analysis showed that BBTV-ZZ DNA 4 is 1 039 nucleotides (nts) in length and this virus could be one member of BBTV Asian group. Transcriptional initiation site A, which is at the 269 nucleotide, was preliminarily determined by using 5' RACE method. BBTV-ZZ DNA 4 non-coding region was sub-cloned by PCR and inserted into upstream of gfp : : gus plant expression vector pCAMBIA 1304 to construct recombinant plasmid pTA2. Agrobacterium tumefaciens harboring pTA2 was injected into leaves of the tobacco (Nicotiana tabacum L. cv. Xanthi NC) via Agrobacterium-infiltration procedure. Transient expressions of GUS and GFP were determined in injected leaves 3 - 5 d later. GUS activities of pTA2, pCAMBIA 1304 injected and non-injected tobacco leaves respectively were 1.007 0 pmol MU(.)mug(-1.)min(-1), 2.069 0 pmol MU(.)mug(-1.)min(-1) and 0.021 4 pmol MU(.)mug(-1.)min(-1). Indirect ELISA for GFP in 1 mg total protein from pTA2, pCAMBIA 1304 injected and non-injected leaves showed an A(490 nm) value of 89.577, 100.440 and 3.287, respectively. These results showed that the non-coding region of BBTV-ZZ DNA 4 has a promoter activity not only in the virus replication in monocot, but also in driving the expression of a foreign gene in dicot plants.
基金Supported by National Natural Science Foundation of China(U14052235)National Natural Science Foundation of Zhangzhou(ZZ2018J03)Shengzhou Environmental Protection Bureau([350600]ZY[GK]2018003-1)
文摘From December 3 to 12,2017,Zhangzhou experienced a haze weather process lasting for 10 days.In this paper,based on surface meteorological observation and environmental monitoring data,statistical comparison,Eta correlation coefficient,clustering analysis and other methods were adopted to analyze the causes of haze weather in the whole process from clean air (December 2) to haze generation,maintenance (December 3-12) and dissipation (December 13).The results showed that two blasts of strong cold air invaded the city during the haze formation and maintenance,and regional pollution transport and static and stable weather were the main reasons for the continuous high concentration of particulate matter,the occurrence of mild pollution from PM 10 and PM 2.5 in some periods and mild-heavy haze weather lasting for 10 days.In this process,PM concentration had a strong negative correlation with air temperature and visibility,and the absolute values of the correlation coefficients were between 0.6 and 0.8.PM concentration correlated with relative humidity positively and with wind speed weakly.Regional transport of particulate matter and ozone was conducted in the form of northeast wind and southeast wind backflow.At night and in the morning,under the influence of static and stable weather and long-term sea and land breeze (6 d),average wind speed was less than 1.5 m/s,and the frequency of static wind and small wind exceeded 50%,while there was no precipitation.At 08:00,in the middle and low altitudes,the inversion layer appeared in one week,and cloud cover was large,while the height of mixed layer was below 600 m.In the whole process,it was in the weak divergence field under cold high pressure,and the overall diffusion,dilution and cleaning ability of the atmosphere were poor.
基金Sponsored by Special Financial Program of Fujian Provincial Department of Finance(K81MLV05A)Program for New Century Excellent Talents in Fujian Province University(NCETFJ)
文摘Taking the construction of Green Lake Eco-park for example, this paper introduced the achievements and experience of this project in rural land expropriation, ecological conservation, humanistic development, and commercial development via field investigation, literature consultation and resident interview, and also problems in the current construction. Then suggestions were put forward to provide references for the further improvement of the Green Lake Eco-park.
基金Project supported by the National Natural Science Foundation of China and the International Atomic Energy Agency R.C. (No. 4850/RI/RB).
文摘Reservoir temperature is an essential parameter in geothermal exploration, which is usually obtained by using chemical geothermometers. There are more than a dozen of conventional geothermometers, each of which has its own limitation for application. Zhangzhou Geothermal Field is a saline water field, the thermal water chemistry of which
