In recent years,artist Yu Xiangzhi has focused on the new outlook of the working people in the new era and created a series of portraits called "Paean to Heroes",presenting working people's enthusiasm an...In recent years,artist Yu Xiangzhi has focused on the new outlook of the working people in the new era and created a series of portraits called "Paean to Heroes",presenting working people's enthusiasm and dedication in the new era with paintings.He hopes that this series of works can resonate with the public,who will pay more attention to the life and working conditions of grassroots workers.Recently,this magazine has interviewed Yu Xiangzhi and talked about the story behind the creation of his "Paean to Heroes"series of oil paintings.展开更多
Zhao Rukuo's Zhu Fan Zhi in the Southern Song dynasty was the first book in the history of China to systematically describe the “Maritime Silk Road”, recording the geography, products, customs, trade, etc. of mo...Zhao Rukuo's Zhu Fan Zhi in the Southern Song dynasty was the first book in the history of China to systematically describe the “Maritime Silk Road”, recording the geography, products, customs, trade, etc. of more than 50 foreign countries and regions. The book included a large amount of medical literature, introducing medical customs and the production of drugs in various places, as well as the efficacy of some medicine. These materials are of unique value for understanding the medicine trade in the Song dynasty, and even the medical culture along the “Maritime Road of Aromatic Medicine”.展开更多
Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical recor...Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical records to study the Xu family’s life and deeds. Xu Chunfu was born in the second year of the Jiajing reign of the Ming Dynasty (1523) and passed away in the 24th year of the Wanli reign of the Ming Dynasty (1596). He served as an official in the Tai Hospital for about 20 years. His work Gu Jin Yi Tong Da Quan was written around the 43rd year of the Jiajing reign of the Ming Dynasty (1556), with a lower limit of the fifth year of the Longqing reign of the Ming Dynasty (1571), and the initial engraving time was not earlier than the eighth year of the Wanli reign of the Ming Dynasty (1580). This paper corrects the shortcomings and errors in previous research, and provides a comprehensive and systematic examination of Xu Chunfu’s life and works.展开更多
Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with oste...Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with osteoporosis and normal subjects from the GEO database and then found SOST with specific expression. We analyzed SOST as an antagonist of the Wnt signaling pathway by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Then we studied the effect of ZGZTF on SOST in Wnt signaling pathway. Osteoporosis model was induced by ovariectomy (OVX) in 8-week-old female Sprague–Dawley (SD) rats. After 12 weeks of treatment with ZGZTF by intragastric administration, the rats were put to death in batch. The changes of alkaline phosphatase (ALP), bone gla protein (BGP) and estradiol (E2) in serum were determined, and bone mineral density (BMD) and histomorphology of right femur were observed. Biomechanics of lumbar vertebra were measured, and the expression of SOST, Wnt3a,β-catenin, LRP5, Runx2, Osx and their mRNA involving the canonical Wnt/β-catenin signaling pathway were detected by Western blot, RT-PCR and Immunohistochemical analysis. All data were analyzed by SPSS 22.0. Results Twelve weeks of treatment with ZGZTF could significantly decrease the level of ALP and BGP in serum, increase the BMD of femurs, and improve the biomechanical capability of vertebral body in maximum loading and elastic modulus. Concerning histomorphology, we found ordered arrangement of trabeculae, slightly thinning of trabeculae and none obvious slight fractures in femurs after 12 weeks of treatment with ZGZTF. The expression of LRP5,β-catenin, Runx2 and Osx involved in the canonical Wnt/β-catenin signaling pathway was significantly up-regulated in the presence of ZGZTF,and the expression of SOST in this pathway was down-regulated. Conclusions These results suggest that ZGZTF may be antiosteoporosis by down-regulating SOST protein to promote Wnt/β-catenin signaling pathway.展开更多
Objective To predict the main active ingredients,potential targets and molecular mechanisms of Yuan Zhi powder in treatment of dementia by using network pharmacology.Methods A database of chemical constituents of Yuan...Objective To predict the main active ingredients,potential targets and molecular mechanisms of Yuan Zhi powder in treatment of dementia by using network pharmacology.Methods A database of chemical constituents of Yuan Zhi powder was constructed by using databases and literatures.Potential targets were predicted by reverse molecular docking,and then a component-target network was constructed.The target network of Alzheimer's disease(AD)was mapped and analyzed to obtain the“active ingredient-AD target”network.The key targets were screened through network analysis.Finally,the rationality of the prediction was analyzed by comparing with the target reported in the literatures.Results There were180chemical constituents acting on the AD target,and the targets included three key targets(cyclooxygenase-2,muscarinic acetylcholine receptor M1,and muscarinic acetylcholine receptor M2)and an important target(acetylcholine esterase).Alzheimer's disease may be treated by regulating the activity of acetylcholine receptors and the binding toβ-amyloid protein,and its biological process may be concentrated in the acetylcholine receptor signal pathway,negative regulation of synaptic transmission and so on.Conclusion The fact that Yuan Zhi powder can treat AD is consistent with the characteristics of multi-components-multitargets-multiple pathways of traditional Chinese medicine.The important targets obtained from network analysis have a large proportion in literature research,so network analysis have some rationality.展开更多
AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups wi...AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups with 20 in each group： control group, CIA group, treatment groups at high dosage and low dosage （GZH and GZL）. CIA was induced by immunization with type Ⅱ collagen （CII） emulsified with equal complete adjuvant at 0.1 mg CII each mouse. Blood lymphocyte suspension was screened for CD4 and CD8 expression using a flow cytometry, the CD4 and CD8 and secretory IgA （sIgA）-positive cells in enteric lamina propria tested with immunohistochemical staining. Tumor necrosis factor-alpha （TNF-α）, interleukin-1 （IL-1）-β, and IL-6 concentrations in serum were assayed with RIA. RESULTS： Gui Zhi decoction can lower the arthritic scores and decrease the occurrence of arthritis. The CD4, CD8, and sIgA-positive cells in CIA mice are less than in control mice, and in Gui Zhi decoction at high dosage could restore the lowered CD4- and CD8-positive cells in lamina propria, and at both high and low dosages could increase the lowered sIgA-positive cells in lamina propria, even still lower than in normal mice. In periphery, the CD4 cells in periphery are higher in CIA mice than in control mice, and Gui Zhi decoction at high and low dosages could decrease the CD4 and CD8 cells. Also, Gui Zhi decoction at high dosage could decrease the IL-6 and TNF-α concentration in serum. CONCLUSION： Gui Zhi decoction can lower the arthritic scores and decrease the incidence of CIA in mice, and the mechanism is in part regulating enteric mucosal immune.展开更多
Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemente...Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemented with varied concentration of different plant growth-regulators, e.g., α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA） in combination with 6-benzylaminopurine （6-BA） and kinetin. The study showed that shoots could be directly regenerated from hypocotyl explants without the intervening callus phase; MS medium was more suitable for adventitious shoots regeneration. The ability of hypocotyls segments to produce shoots varied depending upon their position on the seedlings. The highest regeneration rate was obtained with hypocotyl segments near to the cotyledon cultured on MS basal medium supplemented with 1.5 mg L^-1 6-BA and 0.05 mg L^-1 NAA （63.64%）. The regenerated shoots were readily elongated on the same medium as used for multiplication and rooted on half-strength MS basal medium supplemented with 1.0 mg L^-1 IBA and 100 mg L^-1 activated carbon. After being transferred to greenhouse conditions, 96% of the plantlets were successfully acclimatized. This regeneration system is applied for genetic transformation now.展开更多
This article discusses the development of astronomical tables in ancient China based on the calendrical chapter titled“Lü-li zhi”律曆志(Monograph on harmonics and calendrical astronomy)in official histories.Aft...This article discusses the development of astronomical tables in ancient China based on the calendrical chapter titled“Lü-li zhi”律曆志(Monograph on harmonics and calendrical astronomy)in official histories.After surveying various types of astral scientific tables in ancient China and their layouts,this paper discusses the characteristics and adoption of“licheng”立成tables,a specific kind of pick-up table that seems to have come into use in the Sui period(581–618)and to have been widespread from the Tang period(618–907)onward.The emergence of licheng tables relates largely to the internal development of ancient Chinese astronomy,but they were also probably inspired by auspicial tables and foreign astronomical tables.By comparing tables recorded in the“Lü-li zhi”and the existing licheng tables,we find that most licheng were deleted during the compilation of“Lü-li zhi”to reduce the number of volumes.Moreover,this paper discusses several common solutions used to compress the size of tables in“Lü-li zhi.”The adoption of licheng tables into the ancient Chinese astral sciences and the reformatting of them in“Lü-li zhi”give us a different perspective for understanding the development of ancient Chinese astronomical tables and the compilation of the calendrical portion of official histories.展开更多
Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from t...Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.展开更多
Hongcun is the World Cultural Heritage which is a Traditional Village in China,and Cheng Zhi Hall is the most important traditional dwelling within Hongcun.In December 2010,Yi County Cultural Relics Bureau commissione...Hongcun is the World Cultural Heritage which is a Traditional Village in China,and Cheng Zhi Hall is the most important traditional dwelling within Hongcun.In December 2010,Yi County Cultural Relics Bureau commissioned Anhui Provincial Cultural Relics Protection Center to complete the Restoration Plan of Cheng Zhi Hall,Hongcun Village,Yi County,Huangshan City,Anhui Province,and carried out the actual restoration project from 2015 to 2018.However,by the evaluation of the restoration plan and project of Cheng Zhi Hall,many problems are found such as that the description of restoration measures is rough,the restoration drawings are not clearly marked,and the restoration practices are lack of the theoretical support and so on.Therefore,the aim of this research is to sort out the existing problems,and through the comparative study of the relevant Italian cases,putting forward the optimization strategies of the plan and practice of traditional dwelling conservation practice in China.展开更多
Objective:To evaluate the efficacy and safety of Shen Zhi Ling oral liquid(SZL)alone or combined with basic treatment in treating Alzheimer's Disease(AD).Methods:Databases including PubMed,the Cochrane Library,Emb...Objective:To evaluate the efficacy and safety of Shen Zhi Ling oral liquid(SZL)alone or combined with basic treatment in treating Alzheimer's Disease(AD).Methods:Databases including PubMed,the Cochrane Library,Embase,Web of science,China National Knowledge Infrastructure(CNKI),Wanfang Database and the Chinese Science and Technology Journal Database(VIP)were searched from inception to September 20,2020 to collect relevant randomized controlled trials(RCTs)comparing SZL with other nootropic drugs for AD.The primary clinical outcomes were scores of the Mini Mental State Examination(MMSE)and the Activities of Daily Living(ADL)during treatment.The secondary outcomes of our study included other neuropsychological scale scores including Rapid Verbal Retrieve(RVR),Digit Span(DS),Fuld Object-Memory Evaluation(FOM)and adverse events or reactions.Two authors searched and screened the literature independently,then extracted the data and evaluated the methodological quality by using the handbook of Cochrane Collaboration,and analyzed the data via the Review Manager 5.3 software.Results:In total,we included four studies,covering 217 participants.All studies were in a low methodological quality.The result of MMSE score,ADL score,RVR score,DS score and FOM score in the meta-analysis showed that no statistically significant difference(P>0.05)between the treatment groups and control groups,and the statistical heterogeneity of all meta-analyses was small(I2<50%).SZL was indicated no better than other nootropic drugs.Among the included studies,no study described adverse events.Conclusions:Considering the low quality of the included trials and the different levels of AD patients,we are unable to come to any conclusion about the efficacy and safety of SZL compared with other nootropic drugs.Moreover,we still need rigorously designed,multi-center,large-scale trials to further confirm the efficacy and safety of SZL.展开更多
[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitati...[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.展开更多
[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and...[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.展开更多
文摘In recent years,artist Yu Xiangzhi has focused on the new outlook of the working people in the new era and created a series of portraits called "Paean to Heroes",presenting working people's enthusiasm and dedication in the new era with paintings.He hopes that this series of works can resonate with the public,who will pay more attention to the life and working conditions of grassroots workers.Recently,this magazine has interviewed Yu Xiangzhi and talked about the story behind the creation of his "Paean to Heroes"series of oil paintings.
基金financed by the grants from The National Social Science Fund of China,Late Stage Funding (No. 21FZWB005)。
文摘Zhao Rukuo's Zhu Fan Zhi in the Southern Song dynasty was the first book in the history of China to systematically describe the “Maritime Silk Road”, recording the geography, products, customs, trade, etc. of more than 50 foreign countries and regions. The book included a large amount of medical literature, introducing medical customs and the production of drugs in various places, as well as the efficacy of some medicine. These materials are of unique value for understanding the medicine trade in the Song dynasty, and even the medical culture along the “Maritime Road of Aromatic Medicine”.
基金the 2020 National Social Science Foundation Major Project(20&ZD222)Open Project of Key Laboratory of Xin’an Medical Education in 2022,“Research on the Active Utilization of Intangible Cultural Heritage Resources in the Gu Jin Yi Tong Da Quan"(2022xayx01)2022 Anhui Provincial Central Finance Traditional Chinese Medicine Special Fund“North Huatuo,South Xin’an”Inheritance and Innovation Project:Systematic Compilation and Audiovisual Protection Research of Xin’an Medical Traditional Medicine Intangible Cultural Heritage Project(2022BHTNXA02).
文摘Xu Chunfu was a famous medical expert in the Ming Dynasty, who authored the Gu Jin Yi Tong Da Quan and the Yi Xue Zhi Nan Jie Jing Liu Shu. This paper uses the Xu family genealogy, literature catalog, historical records to study the Xu family’s life and deeds. Xu Chunfu was born in the second year of the Jiajing reign of the Ming Dynasty (1523) and passed away in the 24th year of the Wanli reign of the Ming Dynasty (1596). He served as an official in the Tai Hospital for about 20 years. His work Gu Jin Yi Tong Da Quan was written around the 43rd year of the Jiajing reign of the Ming Dynasty (1556), with a lower limit of the fifth year of the Longqing reign of the Ming Dynasty (1571), and the initial engraving time was not earlier than the eighth year of the Wanli reign of the Ming Dynasty (1580). This paper corrects the shortcomings and errors in previous research, and provides a comprehensive and systematic examination of Xu Chunfu’s life and works.
基金the funding support from the National Natural Science Foundation of China (No. 81573956)Natural Science Foundation of Hunan Province (No. 2018JJ2297)+3 种基金Key Program of Chinese Medicine Science Research Plan of Hunan Province (No. 201612)Key Program of Scientific Research Fund of Hunan Provincial Education Department (No. 16A162)National College Students Innovation and Entrepreneurship Project (No. 201710541002)The Project of Research Learning and Innovative Experiment for College Students in Hunan (No. 2015217, No. 2015220, No. 2016284 and No. 2016281)
文摘Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with osteoporosis and normal subjects from the GEO database and then found SOST with specific expression. We analyzed SOST as an antagonist of the Wnt signaling pathway by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Then we studied the effect of ZGZTF on SOST in Wnt signaling pathway. Osteoporosis model was induced by ovariectomy (OVX) in 8-week-old female Sprague–Dawley (SD) rats. After 12 weeks of treatment with ZGZTF by intragastric administration, the rats were put to death in batch. The changes of alkaline phosphatase (ALP), bone gla protein (BGP) and estradiol (E2) in serum were determined, and bone mineral density (BMD) and histomorphology of right femur were observed. Biomechanics of lumbar vertebra were measured, and the expression of SOST, Wnt3a,β-catenin, LRP5, Runx2, Osx and their mRNA involving the canonical Wnt/β-catenin signaling pathway were detected by Western blot, RT-PCR and Immunohistochemical analysis. All data were analyzed by SPSS 22.0. Results Twelve weeks of treatment with ZGZTF could significantly decrease the level of ALP and BGP in serum, increase the BMD of femurs, and improve the biomechanical capability of vertebral body in maximum loading and elastic modulus. Concerning histomorphology, we found ordered arrangement of trabeculae, slightly thinning of trabeculae and none obvious slight fractures in femurs after 12 weeks of treatment with ZGZTF. The expression of LRP5,β-catenin, Runx2 and Osx involved in the canonical Wnt/β-catenin signaling pathway was significantly up-regulated in the presence of ZGZTF,and the expression of SOST in this pathway was down-regulated. Conclusions These results suggest that ZGZTF may be antiosteoporosis by down-regulating SOST protein to promote Wnt/β-catenin signaling pathway.
基金funding support from the Major new drug creation project (2017ZX09101002-002-008)National Natural Science Foundation of China (81403171)Autonomous Program of China Academy of Chinese Medical Sciences (QZPT001 and 2014065)
文摘Objective To predict the main active ingredients,potential targets and molecular mechanisms of Yuan Zhi powder in treatment of dementia by using network pharmacology.Methods A database of chemical constituents of Yuan Zhi powder was constructed by using databases and literatures.Potential targets were predicted by reverse molecular docking,and then a component-target network was constructed.The target network of Alzheimer's disease(AD)was mapped and analyzed to obtain the“active ingredient-AD target”network.The key targets were screened through network analysis.Finally,the rationality of the prediction was analyzed by comparing with the target reported in the literatures.Results There were180chemical constituents acting on the AD target,and the targets included three key targets(cyclooxygenase-2,muscarinic acetylcholine receptor M1,and muscarinic acetylcholine receptor M2)and an important target(acetylcholine esterase).Alzheimer's disease may be treated by regulating the activity of acetylcholine receptors and the binding toβ-amyloid protein,and its biological process may be concentrated in the acetylcholine receptor signal pathway,negative regulation of synaptic transmission and so on.Conclusion The fact that Yuan Zhi powder can treat AD is consistent with the characteristics of multi-components-multitargets-multiple pathways of traditional Chinese medicine.The important targets obtained from network analysis have a large proportion in literature research,so network analysis have some rationality.
基金Supported by the project of National Natural Science Foundation of China, No. 30171133 and 39870952
文摘AIM： To explore the effect of Gui Zhi decoction on enteric mucosal immune in type Ⅱ collagen-induced arthritis （CIA） in DBA mice. METHODS： Eighty DBA/1, weighing 18-22 g, were randomly divided into four groups with 20 in each group： control group, CIA group, treatment groups at high dosage and low dosage （GZH and GZL）. CIA was induced by immunization with type Ⅱ collagen （CII） emulsified with equal complete adjuvant at 0.1 mg CII each mouse. Blood lymphocyte suspension was screened for CD4 and CD8 expression using a flow cytometry, the CD4 and CD8 and secretory IgA （sIgA）-positive cells in enteric lamina propria tested with immunohistochemical staining. Tumor necrosis factor-alpha （TNF-α）, interleukin-1 （IL-1）-β, and IL-6 concentrations in serum were assayed with RIA. RESULTS： Gui Zhi decoction can lower the arthritic scores and decrease the occurrence of arthritis. The CD4, CD8, and sIgA-positive cells in CIA mice are less than in control mice, and in Gui Zhi decoction at high dosage could restore the lowered CD4- and CD8-positive cells in lamina propria, and at both high and low dosages could increase the lowered sIgA-positive cells in lamina propria, even still lower than in normal mice. In periphery, the CD4 cells in periphery are higher in CIA mice than in control mice, and Gui Zhi decoction at high and low dosages could decrease the CD4 and CD8 cells. Also, Gui Zhi decoction at high dosage could decrease the IL-6 and TNF-α concentration in serum. CONCLUSION： Gui Zhi decoction can lower the arthritic scores and decrease the incidence of CIA in mice, and the mechanism is in part regulating enteric mucosal immune.
文摘Adventitious shoots were successfully regenerated from hypocotyl explants of in vitro cultures of Euonymus japonicus Cu zhi. Hypocotyl slices were cultured on Murashige and Skoog （MS） and B5 basal medium supplemented with varied concentration of different plant growth-regulators, e.g., α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA） in combination with 6-benzylaminopurine （6-BA） and kinetin. The study showed that shoots could be directly regenerated from hypocotyl explants without the intervening callus phase; MS medium was more suitable for adventitious shoots regeneration. The ability of hypocotyls segments to produce shoots varied depending upon their position on the seedlings. The highest regeneration rate was obtained with hypocotyl segments near to the cotyledon cultured on MS basal medium supplemented with 1.5 mg L^-1 6-BA and 0.05 mg L^-1 NAA （63.64%）. The regenerated shoots were readily elongated on the same medium as used for multiplication and rooted on half-strength MS basal medium supplemented with 1.0 mg L^-1 IBA and 100 mg L^-1 activated carbon. After being transferred to greenhouse conditions, 96% of the plantlets were successfully acclimatized. This regeneration system is applied for genetic transformation now.
文摘This article discusses the development of astronomical tables in ancient China based on the calendrical chapter titled“Lü-li zhi”律曆志(Monograph on harmonics and calendrical astronomy)in official histories.After surveying various types of astral scientific tables in ancient China and their layouts,this paper discusses the characteristics and adoption of“licheng”立成tables,a specific kind of pick-up table that seems to have come into use in the Sui period(581–618)and to have been widespread from the Tang period(618–907)onward.The emergence of licheng tables relates largely to the internal development of ancient Chinese astronomy,but they were also probably inspired by auspicial tables and foreign astronomical tables.By comparing tables recorded in the“Lü-li zhi”and the existing licheng tables,we find that most licheng were deleted during the compilation of“Lü-li zhi”to reduce the number of volumes.Moreover,this paper discusses several common solutions used to compress the size of tables in“Lü-li zhi.”The adoption of licheng tables into the ancient Chinese astral sciences and the reformatting of them in“Lü-li zhi”give us a different perspective for understanding the development of ancient Chinese astronomical tables and the compilation of the calendrical portion of official histories.
文摘Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.
基金I would like to extend my sincere gratitude to my supervisor,Maria Cristina Giambruno,for guiding my research.And also,high tribute is paid to prof.Minghao Zhang for supporting my research.
文摘Hongcun is the World Cultural Heritage which is a Traditional Village in China,and Cheng Zhi Hall is the most important traditional dwelling within Hongcun.In December 2010,Yi County Cultural Relics Bureau commissioned Anhui Provincial Cultural Relics Protection Center to complete the Restoration Plan of Cheng Zhi Hall,Hongcun Village,Yi County,Huangshan City,Anhui Province,and carried out the actual restoration project from 2015 to 2018.However,by the evaluation of the restoration plan and project of Cheng Zhi Hall,many problems are found such as that the description of restoration measures is rough,the restoration drawings are not clearly marked,and the restoration practices are lack of the theoretical support and so on.Therefore,the aim of this research is to sort out the existing problems,and through the comparative study of the relevant Italian cases,putting forward the optimization strategies of the plan and practice of traditional dwelling conservation practice in China.
基金This work was supported by the second batch of the“Ten thousand plan”,a national high-level talent special support plan(W02020052).
文摘Objective:To evaluate the efficacy and safety of Shen Zhi Ling oral liquid(SZL)alone or combined with basic treatment in treating Alzheimer's Disease(AD).Methods:Databases including PubMed,the Cochrane Library,Embase,Web of science,China National Knowledge Infrastructure(CNKI),Wanfang Database and the Chinese Science and Technology Journal Database(VIP)were searched from inception to September 20,2020 to collect relevant randomized controlled trials(RCTs)comparing SZL with other nootropic drugs for AD.The primary clinical outcomes were scores of the Mini Mental State Examination(MMSE)and the Activities of Daily Living(ADL)during treatment.The secondary outcomes of our study included other neuropsychological scale scores including Rapid Verbal Retrieve(RVR),Digit Span(DS),Fuld Object-Memory Evaluation(FOM)and adverse events or reactions.Two authors searched and screened the literature independently,then extracted the data and evaluated the methodological quality by using the handbook of Cochrane Collaboration,and analyzed the data via the Review Manager 5.3 software.Results:In total,we included four studies,covering 217 participants.All studies were in a low methodological quality.The result of MMSE score,ADL score,RVR score,DS score and FOM score in the meta-analysis showed that no statistically significant difference(P>0.05)between the treatment groups and control groups,and the statistical heterogeneity of all meta-analyses was small(I2<50%).SZL was indicated no better than other nootropic drugs.Among the included studies,no study described adverse events.Conclusions:Considering the low quality of the included trials and the different levels of AD patients,we are unable to come to any conclusion about the efficacy and safety of SZL compared with other nootropic drugs.Moreover,we still need rigorously designed,multi-center,large-scale trials to further confirm the efficacy and safety of SZL.
文摘[Objectives] To screen TLC conditions of forsythin contained in Jin Weng Zhi Li Granule. [Methods] According to the method described in Chinese Veterinary Pharmacopoeia,thin layer chromatography was used for qualitative identification of forsythin contained in Jin Weng Zhi Li Granule by improving the developing conditions. [Results]After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions]The method is of good feasibility,reproducibility and specificity,and could be used for qualitative detection of forsythin contained in Jin Weng Zhi Li Granule.
基金Supported by Special Project of National Pubic Benefit Industry(Agriculture)(201303040-05)Special Project of Science and Technology Groundwork(2013FYF110600)
文摘[Objectives] The aim of this study was to screen out the TLC conditions of matrine in Jin Weng Zhi Li Granule. [Methods]Referring to the method of Chinese Veterinary Pharmacopoeia,the composition,chromogenic agent and expansion conditions of the developer were optimized,and matrine was qualitatively identified by TLC. [Results] After optimizing TLC conditions of Jin Weng Zhi Li Granule,that is,the thin layer plate was pre-equilibrated in the expansion cylinder for 5 min after spotting,the separation effect of TLC of test samples was favorable. The spot color was clear,reproducibility was good,precision was high,specificity was better. [Conclusions] The method was of good feasibility,reproducibility and specificity,and could be used for qualitative detection of matrine in Jin Weng Zhi Li Granule dampnessheat and diarrhea disease.
