Objective:To investigate the zoonotic visceral leishmaniasis(ZVL) by identification of the most probable reservoir hosts using parasite isolation and analysis of a possible transmission dynamics of the disease in extr...Objective:To investigate the zoonotic visceral leishmaniasis(ZVL) by identification of the most probable reservoir hosts using parasite isolation and analysis of a possible transmission dynamics of the disease in extra-domestic agricultural fields and rural villages.Methods:Rodents were collected from selected study sites in kala-azar endemic areas based on information for localities of kala-azar cases for screening of Leshmania infections using parasitological,serological and polymerase chain reaction(PCR) from March.2013 to January,2014.Ketamine(Clorketam Veterinary) was used to anaesthesize the rodents according the prescribed dosage(average 2 mg/kg for intra-venous route).The blood obtained using sterile needle was dropped into sterile filter paper and allowed to air dry before sealing in plastic bags.The tissues from liver,spleen and skin were macerated in Locke's solution before transferring them into NNN medium.Blood and touch smears of liver,spleen,skin and bone marrow were prepared for fixing using methanol and staining by Giemsa stain for microscopy.These tissues were also ascd for DNA extractions and PCR amplification of Leishmania infection.Results:A total of 335 rodents(13 species) were analyzed by sampling internal organs.The infection rate by PCR was 11.1%(6/54) for Arvicanthis nilothicas compared to 17.6%(3/17) and 12.5%(2/16) for Acomys cahirinus and Tarera(C) robustus respectively.Almost all the infections were found from bone marrow samples(8/48 or 16.7%) compared with 1/91(1.1%) liver,2/87(2.2%) spleen and 0/87(0%) skin.In all study sites with past human VL cases,rodents and proved vectors shared similar habitats.Conclusions:Leishmania donovani might circulate among different species of rodents in kala-azar endemic lowlands and valleys of Ethiopia by Phlebotamus orientalis and Phlebotomus martini.Detailed studies to substantiate the preliminary data on the possible role of these rodents arc urgently needed.展开更多
Background: Visceral leishmaniasis (VL) is one of the most important neglected tropical diseases. Although VL was controlled in several regions of China during the last century, the mountain-type zoonotic visceral lei...Background: Visceral leishmaniasis (VL) is one of the most important neglected tropical diseases. Although VL was controlled in several regions of China during the last century, the mountain-type zoonotic visceral leishmaniasis (MT-ZVL) has reemerged in the hilly areas of China in recent decades. The purpose of this study was to construct an indicator framework for assessing the risk of the MT-ZVL in China, and to provide guidance for preventing disease.Methods: Based on a literature review and expert interview, a 3-level indicator framework was initially established in November 2021, and 28 experts were selected to perform two rounds of consultation using the Delphi method. The comprehensive weight of the tertiary indicators was determined by the Delphi and the entropy weight methods.Results: Two rounds of Delphi consultation were conducted. Four primary indicators, 11 secondary indicators, and 35 tertiary indicators were identified. The Delphi-entropy weight method was performed to calculate the comprehensive weight of the tertiary indicators. The normalized weights of the primary indicators were 0.268, 0.261, 0.242, and 0.229, respectively, for biological factors, interventions, environmental factors, and social factors. The normalized weights of the top four secondary indicators were 0.122, 0.120, 0.098, and 0.096, respectively, for climatic features, geographical features, sandflies, and dogs. Among the tertiary indicators, the top four normalized comprehensive weights were the population density of sandflies (0.076), topography (0.057), the population density of dogs, including tethering (0.056), and use of bed nets or other protective measures (0.056).Conclusions: An indicator framework of transmission risk assessment for MT-ZVL was established using the Delphientropy weight method. The framework provides a practical tool to evaluate transmission risk in endemic areas.展开更多
Zoonotic visceral leishmaniasis(ZVL),caused by the protozoan parasite Leishmania infantum and transmitted to humans and reservoirhosts by female sandflies,is endemic inmany parts of the world(notably in Africa,Asia an...Zoonotic visceral leishmaniasis(ZVL),caused by the protozoan parasite Leishmania infantum and transmitted to humans and reservoirhosts by female sandflies,is endemic inmany parts of the world(notably in Africa,Asia and the Mediterranean).This study presents a new mathematical model for assessing the transmission dynamics of ZVL in human and non-human animal reservoir populations.The model undergoes the usual phenomenon of backward bifurcation exhibited by similar vector-borne disease transmission models.In the absence of such phenomenon(which is shown to arise due to the disease-induced mortality in the host populations),the nontrivial disease-free equilibrium of the model is shown to be globallyasymptotically stable when the associated reproduction number of the model is less than unity.Using case and demographic data relevant to ZVL dynamics in Arac atubamunicipality of Brazil,it is shown,for the default case when systemic insecticide-based drugs are not used to treat infected reservoir hosts,that the associated reproduction number of the model (ℛ0) ranges from 0.3 to 1.4,with a mean of ℛ0=0:85.Furthermore,when the effect of such drug treatment is explicitly incorporated in the model(i.e.,accounting for the additional larval and sandfly mortality,following feeding on the treated reservoirs),the range ofℛ0 decreases toℛ02∈[0.1,0.6],with a mean ofℛ0=0:35(this significantly increases the prospect of the effective control or eliminationof the disease).Thus,ZVL transmissionmodels(in communities where such treatment strategy is implemented)that do not explicitly incorporate the effect of such treatmentmay be over-estimating the disease burden(asmeasured in terms ofℛ0)in the community.It is shown thatℛ0 ismore sensitive to increases in sandflylifespanthan thatof the animal reservoir(so,a strategy that focuses on reducing sandflies,rather than the animal reservoir(e.g.,via culling),may be more effective in reducing ZVL burden in the community).Further sensitivity analysis of themodel ranks the sandfly removal rate(by natural death or by feeding from insecticide-treated reservoir hosts),the biting rate of sandflies on the reservoir hosts and the progression rate of exposed reservoirs to active ZVL as the three parameters with themost effect on the disease dynamics or burden(as measured in terms of the reproduction number ℛ0).Hence,this study identifies the key parameters that play a key role on the disease dynamics,and thereby contributing in the design of effective control strategies(that target the identified parameters).展开更多
Brazil is one of the highest endemic countries for Zoonotic Visceral Leishmaniasis:according to the Brazilian Ministry of Health,the annual number of new human cases and deaths due to this disease has been increasing ...Brazil is one of the highest endemic countries for Zoonotic Visceral Leishmaniasis:according to the Brazilian Ministry of Health,the annual number of new human cases and deaths due to this disease has been increasing for the last 20 years.In addition,regarding the Americas,the specific relationship between canine and human for Visceral Leishmaniasis dynamics is still not well understood.In this work we propose a new model for Zoonotic Visceral Leishmaniasis,based on the models previously published by Burattini et al.(1998)and Ribas et al.(2013).Herein,we modeled the disease dynamics using a modified set of differential equations from those two authors,considering the same assumptions(inclusion of human,dog and sandfly populations,all constants over time).From this set of equations we were able to calculate the basic reproduction number R 0 and to analyze the stability and sensitivity of the system to the parameters variability.As main result,when the stability of the system is reached,the normalized reporting human cases rate is estimated in 9.12E-08/day.This estimation is very close to the 2015 report from Araçatuba city,5.69E-08/day.We also observed from stability and sensitivity analysis that the activity of sandfly population is critical to introduction and maintenance of Zoonotic Visceral Leishmaniasis in the population.In addition,the importance of dog as source of infection concentrates on latent dog,since it does not show clinical symptoms and signs and,therefore,has a great contribution to disease dissemination.As conclusion,considering the presently ethical issues regarding to elimination of positive dog in Brazil and the highly sensitivity of disease dynamics on sandfly population,we recommend that the sandfly population control should be prioritized.展开更多
基金supported by the Bill and Melinda Gates Foundation Global Health Program(Grant number OPPGH5336)Gondar University
文摘Objective:To investigate the zoonotic visceral leishmaniasis(ZVL) by identification of the most probable reservoir hosts using parasite isolation and analysis of a possible transmission dynamics of the disease in extra-domestic agricultural fields and rural villages.Methods:Rodents were collected from selected study sites in kala-azar endemic areas based on information for localities of kala-azar cases for screening of Leshmania infections using parasitological,serological and polymerase chain reaction(PCR) from March.2013 to January,2014.Ketamine(Clorketam Veterinary) was used to anaesthesize the rodents according the prescribed dosage(average 2 mg/kg for intra-venous route).The blood obtained using sterile needle was dropped into sterile filter paper and allowed to air dry before sealing in plastic bags.The tissues from liver,spleen and skin were macerated in Locke's solution before transferring them into NNN medium.Blood and touch smears of liver,spleen,skin and bone marrow were prepared for fixing using methanol and staining by Giemsa stain for microscopy.These tissues were also ascd for DNA extractions and PCR amplification of Leishmania infection.Results:A total of 335 rodents(13 species) were analyzed by sampling internal organs.The infection rate by PCR was 11.1%(6/54) for Arvicanthis nilothicas compared to 17.6%(3/17) and 12.5%(2/16) for Acomys cahirinus and Tarera(C) robustus respectively.Almost all the infections were found from bone marrow samples(8/48 or 16.7%) compared with 1/91(1.1%) liver,2/87(2.2%) spleen and 0/87(0%) skin.In all study sites with past human VL cases,rodents and proved vectors shared similar habitats.Conclusions:Leishmania donovani might circulate among different species of rodents in kala-azar endemic lowlands and valleys of Ethiopia by Phlebotamus orientalis and Phlebotomus martini.Detailed studies to substantiate the preliminary data on the possible role of these rodents arc urgently needed.
基金the National Key Research and Development Program of China(Nos.2021YFC2300800,2021YFC2300804)National Natural Science Foundation of China(No.32161143036).
文摘Background: Visceral leishmaniasis (VL) is one of the most important neglected tropical diseases. Although VL was controlled in several regions of China during the last century, the mountain-type zoonotic visceral leishmaniasis (MT-ZVL) has reemerged in the hilly areas of China in recent decades. The purpose of this study was to construct an indicator framework for assessing the risk of the MT-ZVL in China, and to provide guidance for preventing disease.Methods: Based on a literature review and expert interview, a 3-level indicator framework was initially established in November 2021, and 28 experts were selected to perform two rounds of consultation using the Delphi method. The comprehensive weight of the tertiary indicators was determined by the Delphi and the entropy weight methods.Results: Two rounds of Delphi consultation were conducted. Four primary indicators, 11 secondary indicators, and 35 tertiary indicators were identified. The Delphi-entropy weight method was performed to calculate the comprehensive weight of the tertiary indicators. The normalized weights of the primary indicators were 0.268, 0.261, 0.242, and 0.229, respectively, for biological factors, interventions, environmental factors, and social factors. The normalized weights of the top four secondary indicators were 0.122, 0.120, 0.098, and 0.096, respectively, for climatic features, geographical features, sandflies, and dogs. Among the tertiary indicators, the top four normalized comprehensive weights were the population density of sandflies (0.076), topography (0.057), the population density of dogs, including tethering (0.056), and use of bed nets or other protective measures (0.056).Conclusions: An indicator framework of transmission risk assessment for MT-ZVL was established using the Delphientropy weight method. The framework provides a practical tool to evaluate transmission risk in endemic areas.
文摘Zoonotic visceral leishmaniasis(ZVL),caused by the protozoan parasite Leishmania infantum and transmitted to humans and reservoirhosts by female sandflies,is endemic inmany parts of the world(notably in Africa,Asia and the Mediterranean).This study presents a new mathematical model for assessing the transmission dynamics of ZVL in human and non-human animal reservoir populations.The model undergoes the usual phenomenon of backward bifurcation exhibited by similar vector-borne disease transmission models.In the absence of such phenomenon(which is shown to arise due to the disease-induced mortality in the host populations),the nontrivial disease-free equilibrium of the model is shown to be globallyasymptotically stable when the associated reproduction number of the model is less than unity.Using case and demographic data relevant to ZVL dynamics in Arac atubamunicipality of Brazil,it is shown,for the default case when systemic insecticide-based drugs are not used to treat infected reservoir hosts,that the associated reproduction number of the model (ℛ0) ranges from 0.3 to 1.4,with a mean of ℛ0=0:85.Furthermore,when the effect of such drug treatment is explicitly incorporated in the model(i.e.,accounting for the additional larval and sandfly mortality,following feeding on the treated reservoirs),the range ofℛ0 decreases toℛ02∈[0.1,0.6],with a mean ofℛ0=0:35(this significantly increases the prospect of the effective control or eliminationof the disease).Thus,ZVL transmissionmodels(in communities where such treatment strategy is implemented)that do not explicitly incorporate the effect of such treatmentmay be over-estimating the disease burden(asmeasured in terms ofℛ0)in the community.It is shown thatℛ0 ismore sensitive to increases in sandflylifespanthan thatof the animal reservoir(so,a strategy that focuses on reducing sandflies,rather than the animal reservoir(e.g.,via culling),may be more effective in reducing ZVL burden in the community).Further sensitivity analysis of themodel ranks the sandfly removal rate(by natural death or by feeding from insecticide-treated reservoir hosts),the biting rate of sandflies on the reservoir hosts and the progression rate of exposed reservoirs to active ZVL as the three parameters with themost effect on the disease dynamics or burden(as measured in terms of the reproduction number ℛ0).Hence,this study identifies the key parameters that play a key role on the disease dynamics,and thereby contributing in the design of effective control strategies(that target the identified parameters).
基金Sao Paulo Research Foundation-FAPESP(grant 2011/02633-5 and 2013/13347-9)Mathematics of Information Technology and Complex Systems-Mitacs for partial financial support.
文摘Brazil is one of the highest endemic countries for Zoonotic Visceral Leishmaniasis:according to the Brazilian Ministry of Health,the annual number of new human cases and deaths due to this disease has been increasing for the last 20 years.In addition,regarding the Americas,the specific relationship between canine and human for Visceral Leishmaniasis dynamics is still not well understood.In this work we propose a new model for Zoonotic Visceral Leishmaniasis,based on the models previously published by Burattini et al.(1998)and Ribas et al.(2013).Herein,we modeled the disease dynamics using a modified set of differential equations from those two authors,considering the same assumptions(inclusion of human,dog and sandfly populations,all constants over time).From this set of equations we were able to calculate the basic reproduction number R 0 and to analyze the stability and sensitivity of the system to the parameters variability.As main result,when the stability of the system is reached,the normalized reporting human cases rate is estimated in 9.12E-08/day.This estimation is very close to the 2015 report from Araçatuba city,5.69E-08/day.We also observed from stability and sensitivity analysis that the activity of sandfly population is critical to introduction and maintenance of Zoonotic Visceral Leishmaniasis in the population.In addition,the importance of dog as source of infection concentrates on latent dog,since it does not show clinical symptoms and signs and,therefore,has a great contribution to disease dissemination.As conclusion,considering the presently ethical issues regarding to elimination of positive dog in Brazil and the highly sensitivity of disease dynamics on sandfly population,we recommend that the sandfly population control should be prioritized.
