In this paper,the traditional identification and modern identification technology of Cnidii Fructus and its adulterant were reviewed,and their advantages,disadvantages and practicability were summarized,to provide a r...In this paper,the traditional identification and modern identification technology of Cnidii Fructus and its adulterant were reviewed,and their advantages,disadvantages and practicability were summarized,to provide a reference for the rapid and accurate identification and quality evaluation of Cnidii Fructus.展开更多
Rhizoma Menispermi, derived from the rhizoma of Menispermum dauricum DC., is one of the most popular Chinese medicines. However Rhizoma Menispermi is often illegally mixed with other species in the herbal market, incl...Rhizoma Menispermi, derived from the rhizoma of Menispermum dauricum DC., is one of the most popular Chinese medicines. However Rhizoma Menispermi is often illegally mixed with other species in the herbal market, including Aristolochia mollissimae Hance, which is toxic to the kidneys and potentially carcinogenic. The use of DNA barcoding to authenticate herbs has improved the management and safety of traditional medicines. In this paper, 49 samples belonging to five species, including 34 samples of M. dauricum, from different locations and herb markets in China were collected and identified using DNA barcoding. The sequences of all 34 samples of Rhizoma Menispermi are highly consistent, with only one site variation in internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA and no variations in the psbA-trnH region. The intra-specific genetic distance is much smaller than inter-specific one. Phylogenetic analysis shows that both sequences allow the successful identification of all species. Nearest distance and BLAST1 methods for the ITS2 and psbA-trnH regions indicate 100% identification efficiency. Our research shows that DNA barcoding can effectively distinguish Rhizoma Menispermi from its adulterants from both commercial and original samples, which provides a new and reliable way to monitor commercial herbs and to manage the modern medicine market.展开更多
[Objectives]The research aimed to study the characteristics of Ardisia gigantifolia and its adulterants Embelia scandens and Mappianthus iodoides for identification. [Methods] Through the trait identification method,p...[Objectives]The research aimed to study the characteristics of Ardisia gigantifolia and its adulterants Embelia scandens and Mappianthus iodoides for identification. [Methods] Through the trait identification method,powder microscopic identification method,TLC,inclusions detection and content determination,A. gigantifolia and its adulterants were contrasted. [Results]The morphological and histological characteristics of A. gigantifolia,E. scandens and M. iodoides were different from each other. Containing water,total ash,acid insoluble ash and other inclusions also had difference; the total triterpenoid saponins were respectively 122. 90 mg/g of A. gigantifolia,147. 052 mg/g of E. scandens,and 63. 06 mg/g of M. iodoides. [Conclusions] These methods are accurate and reliable,which could be used for the identification of A. gigantifolia and its adulterants.展开更多
The adulteration concentration of palm kernel oil(PKO)in virgin coconut oil(VCO)was quantified using near-infrared(NIR)hyperspectral imaging.Nowadays,some VCO is adulterated with lower-priced PKO to reduce production ...The adulteration concentration of palm kernel oil(PKO)in virgin coconut oil(VCO)was quantified using near-infrared(NIR)hyperspectral imaging.Nowadays,some VCO is adulterated with lower-priced PKO to reduce production costs,which diminishes the quality of the VCO.This study used NIR hyperspectral imaging in the wavelength region 900-1,650 nm to create a quantitative model for the detection of PKO contaminants(0-100%)in VCO and to develop predictive mapping.The prediction equation for the adulteration of VCO with PKO was constructed using the partial least squares regression method.The best predictive model was pre-processed using the standard normal variate method,and the coefficient of determination of prediction was 0.991,the root mean square error of prediction was 2.93%,and the residual prediction deviation was 10.37.The results showed that this model could be applied for quantifying the adulteration concentration of PKO in VCO.The prediction adulteration concentration mapping of VCO with PKO was created from a calibration model that showed the color level according to the adulteration concentration in the range of 0-100%.NIR hyperspectral imaging could be clearly used to quantify the adulteration of VCO with a color level map that provides a quick,accurate,and non-destructive detection method.展开更多
文摘In this paper,the traditional identification and modern identification technology of Cnidii Fructus and its adulterant were reviewed,and their advantages,disadvantages and practicability were summarized,to provide a reference for the rapid and accurate identification and quality evaluation of Cnidii Fructus.
文摘Rhizoma Menispermi, derived from the rhizoma of Menispermum dauricum DC., is one of the most popular Chinese medicines. However Rhizoma Menispermi is often illegally mixed with other species in the herbal market, including Aristolochia mollissimae Hance, which is toxic to the kidneys and potentially carcinogenic. The use of DNA barcoding to authenticate herbs has improved the management and safety of traditional medicines. In this paper, 49 samples belonging to five species, including 34 samples of M. dauricum, from different locations and herb markets in China were collected and identified using DNA barcoding. The sequences of all 34 samples of Rhizoma Menispermi are highly consistent, with only one site variation in internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA and no variations in the psbA-trnH region. The intra-specific genetic distance is much smaller than inter-specific one. Phylogenetic analysis shows that both sequences allow the successful identification of all species. Nearest distance and BLAST1 methods for the ITS2 and psbA-trnH regions indicate 100% identification efficiency. Our research shows that DNA barcoding can effectively distinguish Rhizoma Menispermi from its adulterants from both commercial and original samples, which provides a new and reliable way to monitor commercial herbs and to manage the modern medicine market.
基金Supported by Scientific Research Program of Guangdong Provincial Administration of Traditional Chinese Medicine(20171282)Zhongshan Science and Technology Plan Project(2017B1094)
文摘[Objectives]The research aimed to study the characteristics of Ardisia gigantifolia and its adulterants Embelia scandens and Mappianthus iodoides for identification. [Methods] Through the trait identification method,powder microscopic identification method,TLC,inclusions detection and content determination,A. gigantifolia and its adulterants were contrasted. [Results]The morphological and histological characteristics of A. gigantifolia,E. scandens and M. iodoides were different from each other. Containing water,total ash,acid insoluble ash and other inclusions also had difference; the total triterpenoid saponins were respectively 122. 90 mg/g of A. gigantifolia,147. 052 mg/g of E. scandens,and 63. 06 mg/g of M. iodoides. [Conclusions] These methods are accurate and reliable,which could be used for the identification of A. gigantifolia and its adulterants.
基金supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D.Program(PHD/0225/2561)the Faculty of Engineering,Kamphaeng Saen Campus,Kasetsart University,Thailand。
文摘The adulteration concentration of palm kernel oil(PKO)in virgin coconut oil(VCO)was quantified using near-infrared(NIR)hyperspectral imaging.Nowadays,some VCO is adulterated with lower-priced PKO to reduce production costs,which diminishes the quality of the VCO.This study used NIR hyperspectral imaging in the wavelength region 900-1,650 nm to create a quantitative model for the detection of PKO contaminants(0-100%)in VCO and to develop predictive mapping.The prediction equation for the adulteration of VCO with PKO was constructed using the partial least squares regression method.The best predictive model was pre-processed using the standard normal variate method,and the coefficient of determination of prediction was 0.991,the root mean square error of prediction was 2.93%,and the residual prediction deviation was 10.37.The results showed that this model could be applied for quantifying the adulteration concentration of PKO in VCO.The prediction adulteration concentration mapping of VCO with PKO was created from a calibration model that showed the color level according to the adulteration concentration in the range of 0-100%.NIR hyperspectral imaging could be clearly used to quantify the adulteration of VCO with a color level map that provides a quick,accurate,and non-destructive detection method.
