Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human...Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells展开更多
Phosphorus is a vital nutrient for algal growth,thus,a better understanding of phosphorus availability is essential to mitigate harmful algal blooms in lakes.Wind waves are a ubiquitous characteristic of lake ecosyste...Phosphorus is a vital nutrient for algal growth,thus,a better understanding of phosphorus availability is essential to mitigate harmful algal blooms in lakes.Wind waves are a ubiquitous characteristic of lake ecosystems.However,its effects on the cycling of organic phosphorus and its usage by phytoplankton remain poorly elucidated in shallow eutrophic lakes.A mesocosm experiment was carried out to investigate the responses of alkaline phosphatase activity fractions to wind waves in large,shallow,eutrophic Lake Taihu.Results showed that wind-driven waves induced the release of alkaline phosphatase and phosphorus from the sediment,and dramatically enhanced phytoplanktonic alkaline phosphatase activity.However,compared to the calm conditions,bacterial and dissolved alkaline phosphatase activity decreased in wind-wave conditions.Consistently,the gene copies of Microcystis phoX increased but bacterial phoX decreased under wind-wave conditions.The ecological effects of these waves on phosphorus and phytoplankton likely accelerated the biogeochemical cycling of phosphorus and promoted phytoplankton production in Lake Taihu.This study provides an improved current understanding of phosphorus availability and the phosphorus strategies of plankton in shallow,eutrophic lakes.展开更多
This study investigated the osteogenic performance of new brushite cements obtained from Li+-dopedβ-tricalcium phosphate as a promising strategy for bone regeneration.Lithium(Li+)is a promising trace element to encou...This study investigated the osteogenic performance of new brushite cements obtained from Li+-dopedβ-tricalcium phosphate as a promising strategy for bone regeneration.Lithium(Li+)is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells(hASCs)and the osteogenic differentiation-related gene expression,essential for osteogenesis.In-situ X-ray diffraction(XRD)and in-situ 1H nuclear magnetic resonance(1H NMR)measurements proved the precipitation of brushite,as main phase,and monetite,indicating that Li+favored the formation of monetite under certain conditions.Li+was detected in the remaining pore solution in significant amounts after the completion of hydration.Isothermal calorimetry results showed an accelerating effect of Li+,especially for low concentration of the setting retarder(phytic acid).A decrease of initial and final setting times with increasing amount of Li+was detected and setting times could be well adjusted by varying the setting retarder concentration.The cements presented compressive mechanical strength within the ranges reported for cancellous bone.In vitro assays using hASCs showed normal metabolic and proliferative levels.The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li+for increasing the in vivo bone density.The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies.展开更多
文摘Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells
基金the National Natural Science Foundation of China(Nos.41701098,41621002,and 41830757)the Major Science and Technology Program for Water Pollution Control and Treatment(No.2018ZX07701001-24)“One-Three-Five”Strategic Planning of Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences(Nos.NIGLAS2017GH03,NIGLAS2017GH04,and NIGLAS2017GH05)。
文摘Phosphorus is a vital nutrient for algal growth,thus,a better understanding of phosphorus availability is essential to mitigate harmful algal blooms in lakes.Wind waves are a ubiquitous characteristic of lake ecosystems.However,its effects on the cycling of organic phosphorus and its usage by phytoplankton remain poorly elucidated in shallow eutrophic lakes.A mesocosm experiment was carried out to investigate the responses of alkaline phosphatase activity fractions to wind waves in large,shallow,eutrophic Lake Taihu.Results showed that wind-driven waves induced the release of alkaline phosphatase and phosphorus from the sediment,and dramatically enhanced phytoplanktonic alkaline phosphatase activity.However,compared to the calm conditions,bacterial and dissolved alkaline phosphatase activity decreased in wind-wave conditions.Consistently,the gene copies of Microcystis phoX increased but bacterial phoX decreased under wind-wave conditions.The ecological effects of these waves on phosphorus and phytoplankton likely accelerated the biogeochemical cycling of phosphorus and promoted phytoplankton production in Lake Taihu.This study provides an improved current understanding of phosphorus availability and the phosphorus strategies of plankton in shallow,eutrophic lakes.
基金funded by the Portuguese Foundation for Science and Technology(FCT)and the German Academic Exchange Service(Deutscher Akademischer Austauschdienst,DAAD)for the transnational cooperation FCT/DAAD 2018-2019FRM acknowledges her contract under the Transitional Rule DL 57/2016(CTTI-57/18-I3BS(5))attributed by the FCT.VPR acknowledges the Junior Researcher contracts(POCI-01-0145-FEDER-031367+1 种基金POCI-01-0145-FEDER-029139)under the projects Fun4TE project(PTDC/EMD-EMD/31367/2017)and B-Liver(PTDC/EMD-EMD/29139/2017)attributed by the FCT.The authors also thank the funds provided under the distinctions attributed to JMO(IF/01285/2015)and SP(CEECIND/03673/2017).Furthermore,funding by the German Research Foundation(Deutsche Forschungsgemeinschaft,DFG),Grant Nr.HU 2498/1-1GB 1/22-1,is acknowledged.
文摘This study investigated the osteogenic performance of new brushite cements obtained from Li+-dopedβ-tricalcium phosphate as a promising strategy for bone regeneration.Lithium(Li+)is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells(hASCs)and the osteogenic differentiation-related gene expression,essential for osteogenesis.In-situ X-ray diffraction(XRD)and in-situ 1H nuclear magnetic resonance(1H NMR)measurements proved the precipitation of brushite,as main phase,and monetite,indicating that Li+favored the formation of monetite under certain conditions.Li+was detected in the remaining pore solution in significant amounts after the completion of hydration.Isothermal calorimetry results showed an accelerating effect of Li+,especially for low concentration of the setting retarder(phytic acid).A decrease of initial and final setting times with increasing amount of Li+was detected and setting times could be well adjusted by varying the setting retarder concentration.The cements presented compressive mechanical strength within the ranges reported for cancellous bone.In vitro assays using hASCs showed normal metabolic and proliferative levels.The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li+for increasing the in vivo bone density.The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies.
