In order to extract peptides from formalin-fixed tissue and compare the peptide profile differences between control group and disease group,different antigen retrieval(AR) methods were investigated in this paper: orga...In order to extract peptides from formalin-fixed tissue and compare the peptide profile differences between control group and disease group,different antigen retrieval(AR) methods were investigated in this paper: organic solvent,trypsin and magnetic bead.MALDI-TOF MS was used for evaluating the retrieval efficiency.Results showed: trypsin retrieval method was compatible to MS analysis and the higher quality spectra could be acquired,the time of digestion did not affect the peptide profile but the concentration was crucial.We concluded the optimal conditions as follows: digestion with 0.1μg/μL of trypsin at 37℃ for 2h and using the of α-cyano-4-hydroxy cinnamic acid as the MALDI matrix.展开更多
文摘In order to extract peptides from formalin-fixed tissue and compare the peptide profile differences between control group and disease group,different antigen retrieval(AR) methods were investigated in this paper: organic solvent,trypsin and magnetic bead.MALDI-TOF MS was used for evaluating the retrieval efficiency.Results showed: trypsin retrieval method was compatible to MS analysis and the higher quality spectra could be acquired,the time of digestion did not affect the peptide profile but the concentration was crucial.We concluded the optimal conditions as follows: digestion with 0.1μg/μL of trypsin at 37℃ for 2h and using the of α-cyano-4-hydroxy cinnamic acid as the MALDI matrix.
