Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to sc...Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesisrelated gene identified in the F_(2) population derived from the cross between cotton cultivars Xinluzao 61(resistant to aphid) and Xinluzao 50(susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds(LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated GhLAC4-3. Silencing of GhLAC4-3 in Xinluzao 61 via virus-induced gene silencing(VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that GhLAC4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton.展开更多
<div style="text-align:justify;"> <i><span style="font-family:Verdana;">Sipha</span></i><span style="font-family:Verdana;"> <i>maydis</i>&l...<div style="text-align:justify;"> <i><span style="font-family:Verdana;">Sipha</span></i><span style="font-family:Verdana;"> <i>maydis</i></span><span style="font-family:""><span style="font-family:Verdana;"> Passerini (Hemiptera: Aphididae) is a pest of cereals in many regions of the world and was identified as an invasive pest of the US in 2007. Regional surveys from 2015-2017 revealed this pest was broadly distributed throughout many of the western Great Plains states where it is a potential threat to cereal production. The common name hedgehog grain aphid, HGA, has been associated with </span><i><span style="font-family:Verdana;">Sipha</span></i> <i><span style="font-family:Verdana;">maydis</span></i><span style="font-family:Verdana;"> in the US. Cross-resistance where a plant is resistant to one aphid species and is also resistant to another species</span></span><span style="font-family:Verdana;"> that</span><span style="font-family:""><span style="font-family:Verdana;"> is known to occur. Six barleys were evaluated for cross-resistance to HGA: Russian wheat aphid, RWA, resistant germplasms STARS 9301B and STARS 9577B and cultivar “Mesa”;greenbug, GB, resistant germplasm STARS 1501B and cultivar “Post 90”;and RWA and GB resistant experimental line 00BX 11-115. Cultivars “Morex” and “Schuyler” were susceptible controls. Antixenosis was measured 5 days after infestation by HGA. Seedling damage ratings and reductions in seedling growth were recorded after 17 days of infestation. Intrinsic rate of increase, </span><i><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">m</span></sub></i><span style="font-family:Verdana;">, of HGA was determined by following the development of newborn aphids to adulthood and reproduction. 00BX 11-115 and Post 90 had significantly greater antixenosis (fewer aphids/seedling), significantly lower plant damage ratings, and significantly lower intrinsic rates of increase than other entries. Differences in seedling growth were not significant. 00BX 11-115 and Post 90 were the only entries with the </span><i><span style="font-family:Verdana;">Rsg</span></i><span style="font-family:Verdana;">1 greenbug resistance gene. </span><i><span style="font-family:Verdana;">Rsg</span></i><span style="font-family:Verdana;">1 greenbug resistance confers cross-resistance to HGA in the seedling stage.</span></span><span style="font-family:Verdana;"></span> </div>展开更多
基金supported by the Corps Science and Technology Innovation Talent Plan (2021CB028)the Shihezi Science and Technology Research Key Field Science and Technology Research Project (2022NY01)+1 种基金the Fifth Division Science and Technology Plan Project (2021NY02)the Young and Middle-aged Leading Talent Plan (2020CB017)。
文摘Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesisrelated gene identified in the F_(2) population derived from the cross between cotton cultivars Xinluzao 61(resistant to aphid) and Xinluzao 50(susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds(LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated GhLAC4-3. Silencing of GhLAC4-3 in Xinluzao 61 via virus-induced gene silencing(VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that GhLAC4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton.
文摘<div style="text-align:justify;"> <i><span style="font-family:Verdana;">Sipha</span></i><span style="font-family:Verdana;"> <i>maydis</i></span><span style="font-family:""><span style="font-family:Verdana;"> Passerini (Hemiptera: Aphididae) is a pest of cereals in many regions of the world and was identified as an invasive pest of the US in 2007. Regional surveys from 2015-2017 revealed this pest was broadly distributed throughout many of the western Great Plains states where it is a potential threat to cereal production. The common name hedgehog grain aphid, HGA, has been associated with </span><i><span style="font-family:Verdana;">Sipha</span></i> <i><span style="font-family:Verdana;">maydis</span></i><span style="font-family:Verdana;"> in the US. Cross-resistance where a plant is resistant to one aphid species and is also resistant to another species</span></span><span style="font-family:Verdana;"> that</span><span style="font-family:""><span style="font-family:Verdana;"> is known to occur. Six barleys were evaluated for cross-resistance to HGA: Russian wheat aphid, RWA, resistant germplasms STARS 9301B and STARS 9577B and cultivar “Mesa”;greenbug, GB, resistant germplasm STARS 1501B and cultivar “Post 90”;and RWA and GB resistant experimental line 00BX 11-115. Cultivars “Morex” and “Schuyler” were susceptible controls. Antixenosis was measured 5 days after infestation by HGA. Seedling damage ratings and reductions in seedling growth were recorded after 17 days of infestation. Intrinsic rate of increase, </span><i><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">m</span></sub></i><span style="font-family:Verdana;">, of HGA was determined by following the development of newborn aphids to adulthood and reproduction. 00BX 11-115 and Post 90 had significantly greater antixenosis (fewer aphids/seedling), significantly lower plant damage ratings, and significantly lower intrinsic rates of increase than other entries. Differences in seedling growth were not significant. 00BX 11-115 and Post 90 were the only entries with the </span><i><span style="font-family:Verdana;">Rsg</span></i><span style="font-family:Verdana;">1 greenbug resistance gene. </span><i><span style="font-family:Verdana;">Rsg</span></i><span style="font-family:Verdana;">1 greenbug resistance confers cross-resistance to HGA in the seedling stage.</span></span><span style="font-family:Verdana;"></span> </div>
