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Modifications in aroma characteristics of'Merlot'dry red wines aged in American,French and Slovakian oak barrels with different toasting degrees
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作者 Haocheng Lu Binhao Cheng +2 位作者 Yibin Lan Changqing Duan Fei He 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期381-391,共11页
Aging in oak barrels is widely used in enology which could bring flavor changes and aromatic complexity to wines.In the present study,the aroma compounds were analyzed from the‘Merlot’dry red wines,which were fermen... Aging in oak barrels is widely used in enology which could bring flavor changes and aromatic complexity to wines.In the present study,the aroma compounds were analyzed from the‘Merlot’dry red wines,which were fermented in two types of fermenters(stainless steel tank and rotated oak barrel)and aged in six types of oak barrels(three geographic origins×two toasting degrees)for different time(0,3,6 and 9 months,respectively).Results showed that 30 volatiles were associated with barrels and increased during oak aging.The fermenters could influence the intensities of the toast,leathery,smoky,fruity,floral and caramel aromas.The concentration of whisky lactone,eugenol,cis-isoeugenol,and the intensities of the toast and spicy aromas were highest in the wines aged in American oak and were lowest in the wines aged in French oak barrels.The concentrations of guaiacol,syringol,trans-isoeugenol,furfural alcohol,vanilla,cis-whisky lactone enabled the medium toasting barrels to be distinguished from the light toasting ones.The compounds originating from the barrels could be used to distinguish the types of different barrels,but the other general grape-derived and fermentation-derived volatiles could not.The fermenters,oak species and toasting degrees of the barrels all had significant effects on the aroma profiles of the aged‘Merlot’dry red wines,but the influence of the geographic origin was not obvious. 展开更多
关键词 Wine aroma Oak Barrel Oak species Toasting degrees Geographic origin Aging
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Stabilizing perovskite precursors with the reductive natural amino acid for printable mesoscopic perovskite solar cells
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作者 Wenjing Hu Jian Yang +9 位作者 Chuang Yang Xufeng Xiao Chaoyang Wang Zhaozhen Cui Qiaojiao Gao Jianhang Qi Minghao Xia Yaqiong Su Anyi Mei Hongwei Han 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第3期32-39,I0003,共9页
Solution processability significantly advances the development of highly-efficient perovskite solar cells.However,the precursor solution tends to undergo irreversible degradation reactions,impairing the device perform... Solution processability significantly advances the development of highly-efficient perovskite solar cells.However,the precursor solution tends to undergo irreversible degradation reactions,impairing the device performance and reproducibility.Here,we utilize a reductive natural amino acid,Nacetylcysteine(NALC),to stabilize the precursor solution for printable carbon-based hole-conductorfree mesoscopic perovskite solar cells.We find that I_(2) can be generated in the aged solution containing methylammonium iodide(MI) in an inert atmosphere and speed up the MA-FA^(+)(formamidinium) reaction which produces large-size cations and hinders the formation of perovskite phase.NALC effectively stabilizes the precursor via its sulfhydryl group which reduces I_(2) back to I^(-)and provides H^(+).The NALC-stabilized precursor which is aged for 1440 h leads to devices with a power conversion efficiency equivalent to 98% of that for devices prepared with the fresh precursor.Furthermore,NALC improves the device power conversion efficiency from 16.16% to 18.41% along with enhanced stability under atmospheric conditions by modifying grain boundaries in perovskite films and reducing associated defects. 展开更多
关键词 Perovskite solar cells Perovskite precursor Degradation STABILIZATION Reductive natural amino acid
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Effect of proteases on water-soluble and fat-soluble aroma precursors of goat visceras protein hydrolysate
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作者 Ana Rita Ribeiro de Araújo Cordeiro Lorena Lucena de Medeiros +5 位作者 Lary de Souza Olegário Leila Moreira de Carvalho Taliana Kenia Alencar Bezerra Maria Teresa Bertoldo Pacheco Mércia de Sousa Galvão Marta Suely Madruga 《Food Bioscience》 SCIE 2022年第3期975-985,共11页
This study investigated the action of proteolytic enzymes in the production of goat viscera hydrolysates,and their flavoring potential.The study focus the production of flavor precursor molecules and the formation of ... This study investigated the action of proteolytic enzymes in the production of goat viscera hydrolysates,and their flavoring potential.The study focus the production of flavor precursor molecules and the formation of volatile compounds resulting from four hydrolysis treatments:HA-Alcalase®,HF-Flavourzyme®,HN-Neutrase®,and HFN-Flavourzyme® and Neutrase®.The highest degree of hydrolysis (26.74%),number (23 volatiles) and concentration of volatile compounds (274.0 μg/L) were obtained with the action of Alcalase.The main classes of volatile compounds identified in hydrolysates included acids,aldehydes,pyrazines,terpenes and Streacker aldehydes.Amino acids,sugars and fatty acids were identified with higher concentrations in HF and HFN hydrolysates due to the low participation of these precursors in the formation of volatile compounds.Therefore,the use of Alcalase in the proteolysis of goat viscera stood out compared to other enzymes,providing impacting aroma compounds,such as pyrazines,for meat flavor. 展开更多
关键词 aroma precursors FLAVORING Goat byproducts Proteases Enzymatic hydrolysates Volatile compounds
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Plant-Derived Enzymes Producing Chiral Aroma Compounds and Potential Application 被引量:1
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作者 Fang Dong Qian Fan +1 位作者 Xinguo Su Lanting Zeng 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第2期383-398,共16页
Aroma(volatile)compounds play important ecological functions in plants,and also contribute to the quality of plant-derived foods.Moreover,chiral aroma compounds affect their functions in plants and lead to different f... Aroma(volatile)compounds play important ecological functions in plants,and also contribute to the quality of plant-derived foods.Moreover,chiral aroma compounds affect their functions in plants and lead to different flavor quality properties.Formations of chiral aroma compounds are due to the presence of enzymes producing these compounds in plants,which are generally involved in the final biosynthetic step of the aroma compounds.Here,we review recent progress in research on the plant-derived enzymes producing chiral aroma compounds,and their changes in response to environmental factors.The chiral aroma enzymes that have been reported produce(R)-linalool,(S)-linalool,(R)-limonene,and(S)-limonene,etc.,and these enzymes are found in various plant species.We also discuss the origins of enantioselectivity in the plant-derived enzymes producing chiral aroma compounds and summarize the potential use of plants containing enzymes producing chiral aroma compounds for producing chiral flavors/fragrances. 展开更多
关键词 aroma CHIRAL ENANTIOSELECTIVITY ENZYME plant volatile STEREOCHEMISTRY
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Ionospheric precursors of strong earthquakes observed using six GNSS stations data during continuous five years(2011-2015) 被引量:1
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作者 H.E.Eshkuvatov B.J.Ahmedov +3 位作者 Y.A.Tillayev M.Arslan Tariq M.Ali Shah Libo Liu 《Geodesy and Geodynamics》 CSCD 2023年第1期65-79,共15页
This study reports the morphological characteristics of anomalous variations in Global Navigation Satellite System Total Electron Content(GNSS-TEC) prior to the strong local earthquakes(EQ) that occurred during the pe... This study reports the morphological characteristics of anomalous variations in Global Navigation Satellite System Total Electron Content(GNSS-TEC) prior to the strong local earthquakes(EQ) that occurred during the period of 2011-2015.We have analyzed 20 earthquakes of magnitude M> 5.6.A statistical technique is implemented on the data of six GNSS stations located in Tashkent,Kitab,and Maidanak in Uzbekistan,and Islamabad,Multan,Quetta in Pakistan.The results show continuous anomalous variations in TEC during 24 h befo re the occupancy of local earthquakes.It is shown that the precursors before the occurrence of strong earthquakes,in particular of magnitude 5.7,7.7,7.5,7.8 and 7.3 are detected near Eastern Uzbekistan(26 May 2013),Southwestern Pakistan(24 September 2013),Hindukush region of Afghanistan(26 October 2015),and Central Nepal(25 April 2015) and(12 May 2015),respectively.The ionospheric anomalies appearing before the strong earthquakes at six GNSS stations are registered in 14cases(70%) out of 20 selected EQs.It is depicted that anomalies referred to as ionospheric precursors appeared about 1-7 days prior to the occurrence of strong earthquakes. 展开更多
关键词 GNSS TEC EARTHQUAKES Ionospheric precursors
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Effect of cooking processes on tilapia aroma and potential umami perception 被引量:1
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作者 Danni Zhang Charfedinne Ayed +1 位作者 Ian D.Fisk Yuan Liu 《Food Science and Human Wellness》 SCIE CSCD 2023年第1期35-44,共10页
Tilapia is a freshwater fish group with a sustainable prospect but suffers off-notes appearing during cooking processes.To promote pleasant odorants by thermal cooking processes,tilapia fillets were cooked in differen... Tilapia is a freshwater fish group with a sustainable prospect but suffers off-notes appearing during cooking processes.To promote pleasant odorants by thermal cooking processes,tilapia fillets were cooked in different ways(roasting,microwave-heating,boiling and steaming).Their aroma profiles were analysed with special focus on off-notes and umami-enhancing odorants by principal component analysis,and correlated with the heating time,colour,moisture and water activity by partial least squares regression analysis.Results showed that the“green”and“earthy”off-notes were highly correlated with the boiling process(excess of water,short heating time),while most of the umami-enhancing odorants had a high association with the roasting process(low water content,long heating time,better Maillard reaction).This study indicated that roasting is the most adapted cooking process promoting Maillard-derived aromas,umami-enhancing aromas and meanwhile,reducing off-notes.This research helps in understanding the off-note generation in tilapia and promoting desirable umami-enhancing odorants. 展开更多
关键词 TILAPIA Thermal cooking process aroma Off-note UMAMI
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Complexation with pre-formed“empty”V-type starch for encapsulation of aroma compounds
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作者 Jingyi Zhou Lingyan Kong 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期488-494,共7页
Aroma compounds are low-molecular-weight organic volatile molecules and are broadly utilized in the food industry.However,due to their high volatility and evaporative losses during processing and storage,the stabiliza... Aroma compounds are low-molecular-weight organic volatile molecules and are broadly utilized in the food industry.However,due to their high volatility and evaporative losses during processing and storage,the stabilization of these volatile ingredients using encapsulation is a commonly investigated practice.Complexation of aroma compounds using starch inclusion complex could be a potential approach due to the hydrophobicity of the left-handed single helical structure.In the present study,we used starch of three different V-type structures,namely V,V,and V,to encapsulate six different aroma compounds,including1-decanol(DN),cis-3-hexen-1-ol(HN),4-allylanisole(AN),γ-decalactone(DA),trans-cinnamaldehyde(CA),and citral(CT).The formed inclusion complexes samples were characterized using complementary techniques,including X-ray diffraction(XRD)and differential scanning calorimetry(DSC).The results showed that upon complexation with aroma compounds,all V-subtypes retained their original crystalline structures.However,different trends of crystallinity were observed for each type of the prepared inclusion complexes.Additionally,among three V-type starches,V-type starch formed inclusion complexes with aroma compounds most efficiently and promoted the formation of FormⅡcomplex.This study suggested that the structure of aroma compounds and the type of V starch could both affect the complexation properties. 展开更多
关键词 STARCH “Empty”V-type aroma Inclusion complex ENCAPSULATION
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The role of flavonoids in mitigating food originated heterocyclic aromatic amines that concerns human wellness
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作者 Hui Teng Hongting Deng +3 位作者 Chang Zhang Hui Cao Qun Huang Lei Chen 《Food Science and Human Wellness》 SCIE CSCD 2023年第4期975-985,共11页
Meat products are an important part in our daily diet,providing valuable nutrients for the human body.However,heating processes cause the meat to become more appetizing with changes in texture,appearance,flavor,and ch... Meat products are an important part in our daily diet,providing valuable nutrients for the human body.However,heating processes cause the meat to become more appetizing with changes in texture,appearance,flavor,and chemical properties by the altering of protein structure and other ingredients.As one kind of cooking-induced contaminants,heterocyclic aromatic amines(HAAs)are widely present in protein aceous food products with strong carcinogenic and mutagenic properties.In order to promote the safety of traditional meat products,this review focused on the formation,metabolism,biological monitoring and inhibitory mechanism of HAA.An overview of the formation pathways,hazards,and control methods of HAAs during food processing in recent years was studied,aiming to provide some valuable information for exploring effective methods to inhibit the production of associated hazards during food processing.Systematic selection of different types of flavonoids to explore their effects on the formation of HAAs in an actual barbecue system can provide theoretical reference for effectively controlling the formation of HAAs and reducing their harm to human health. 展开更多
关键词 Heterocyclic aromatic amines FLAVONOIDS Hazardous control precursors
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Not Follicular but Dermal Melanocyte Precursors Are Histopathologically Retained in Vitiligo
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作者 Atsushi Tanemura Ryoko Kawabe +5 位作者 Yuka Sudo Kazunori Yokoi Takanobu Kabasawa Mitsuru Futakuchi Tamio Suzuki Manabu Fujimoto 《Journal of Cosmetics, Dermatological Sciences and Applications》 CAS 2023年第1期38-44,共7页
Although perifollicular repigmentation in the vitiligo lesions is owing to activation of follicular melanocyte stem cells and/or precursor cells followed by supplying matured melanocytes, the underlying mechanism of d... Although perifollicular repigmentation in the vitiligo lesions is owing to activation of follicular melanocyte stem cells and/or precursor cells followed by supplying matured melanocytes, the underlying mechanism of diffuse repigmentation on the whole vitiligo surface remains still unknown. In addition to the presence of remaining melanocytes, it is conceivable that dermal melanocyte precursor cells contribute to induce diffuse repigmentation after treatment. Therefore, we investigated here whether dermal and follicular melanocyte precursor cells were reduced or not in vitiligo lesions. We performed an immunostaining for Nestin and p75NGFR as dermal melanocyte precursor cells and MITF/Fzd4 as follicular melanocyte precursor cells and compared the positive cells number between lesions and non-lesions (n = 11). Although MITF<sup>+</sup>/Fzd4<sup>+</sup> cells in the hair follicle were significantly decreased in number in the lesions, Nestin<sup>+</sup> and p75NGFR<sup>+</sup> cells were not. This result indicates that dermal precursor cells could be retained in the vitiligo lesions but be disturbed to differentiate into matured melanocytes. 展开更多
关键词 Melanocyte Precursor Cells p75NGFR NESTIN Frizzled4 VITILIGO
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Effects of post-mortem aging process on characteristic water-soluble taste-active precursors in yellow-feathered broilers
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作者 Na Xu Xianming Zeng +4 位作者 Lingyun Li Xinyue Zhang Peng Wang Minyi Han Xinglian Xu 《Food Science and Human Wellness》 SCIE CSCD 2023年第1期242-253,共12页
Chilled chicken has become the mainstream of chicken consumption.In order to explore the effect of post-mortem aging on water-soluble flavor precursors of chicken,pH,adenosine triphosphate(ATP)degradation,flavor nucle... Chilled chicken has become the mainstream of chicken consumption.In order to explore the effect of post-mortem aging on water-soluble flavor precursors of chicken,pH,adenosine triphosphate(ATP)degradation,flavor nucleosides,free amino acids and water-soluble low molecular weight peptides were determined using Qingyuan partridge yellow-feathered broilers as material during 0-4℃ post-mortem aging in 48 h.The results showed that the pH value fell to the limit pH 5.64(4 h)in chicken breast and 6.21(3 h)in thigh.Regardless of chicken breast or thigh,ATP dropped rapidly within 3 h.It was found that the K-value in chicken thigh was the lowest at 2 h indicating the freshness was the best.Considering the equivalent umami concentration(EUC),the value at 3 h and 4 h was relatively high,but the corresponding electronic tongue umami value was not high,which further showed that the water-soluble low molecular taste peptide played an important role on the post-mortem aging process.Combined with cluster analysis and partial least squares discriminant analysis(PLS-DA),it was preliminarily inferred that the optimal time for chilled chicken during 0-4℃ post-mortem aging was 2 h,which could provide a theoretical basis for the further processing of fresh chicken. 展开更多
关键词 CHICKEN POST-MORTEM Flavor precursors Low molecular weight peptides(LMWPs)
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An Attempt to Analyze a Human Nervous System Algorithm for Sensing Earthquake Precursors
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作者 Da Cao 《Open Journal of Earthquake Research》 2023年第1期1-25,共25页
We statistically validate the 2011-2022 earthquake prediction records of Ada, the sixth finalist of the 2nd China AETA in 2021, who made 147 earthquake predictions (including 60% of magnitude 5.5 earthquakes) with a p... We statistically validate the 2011-2022 earthquake prediction records of Ada, the sixth finalist of the 2nd China AETA in 2021, who made 147 earthquake predictions (including 60% of magnitude 5.5 earthquakes) with a prediction accuracy higher than 70% and a confidence level of 95% over a 12-year period. Since the reliable earthquake precursor signals described by Ada and the characteristics of Alfvén waves match quite well, this paper proposes a hypothesis on how earthquakes are triggered based on the Alfvén (Q G) torsional wave model of Gillette et al. When the plume of the upper mantle column intrudes into the magma and lithosphere of the soft flow layer during the exchange of hot and cold molten material masses deep inside the Earth’s interior during ascent and descent, it is possible to form body and surface plasma sheets under certain conditions to form Alfven nonlinear isolated waves, and Alfven waves often perturb the geomagnetic field, releasing huge heat and kinetic energy thus triggering earthquakes. To explain the complex phenomenon of how Ada senses Alvfen waves and how to locate epicenters, we venture to speculate that special magnetosensory cells in a few human bodies can sense earthquake precursors and attempt to hypothesize an algorithm that analyzes how the human biological nervous system encodes and decodes earthquake precursors and explains how human magnetosensory cells can solve complex problems such as predicting earthquake magnitude and locating epicenters. 展开更多
关键词 Earthquake Prediction Earthquake precursors Mantle Column Plume ASTHENOSPHERE Alfven Isolated Waves Human Magnetic Induction Cells Neuronal Spikes Bayesian Algorithm
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不同酵母发酵的白兰地基酒在蒸馏前后的香气分析
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作者 马玥 吴元 +5 位作者 张葆春 申春华 于立娜 巩传斌 李成林 唐柯 《食品与发酵工业》 CAS CSCD 北大核心 2024年第7期250-258,I0006-I0013,共17页
本研究旨在探究以不同酵母(71B、KD、F33、K1、FC9DP、D254、FC9)酿造的新疆产区的白兰地基酒及其新蒸馏白兰地原酒在挥发性风味物质及香气特征的差异。通过对不同样品进行全二维气相色谱-飞行时间质谱分析结合感官定量描述分析。结果显... 本研究旨在探究以不同酵母(71B、KD、F33、K1、FC9DP、D254、FC9)酿造的新疆产区的白兰地基酒及其新蒸馏白兰地原酒在挥发性风味物质及香气特征的差异。通过对不同样品进行全二维气相色谱-飞行时间质谱分析结合感官定量描述分析。结果显示,添加酵母D254的基酒香气物质总量最高,果香、花香、木质香气特征更突出;在蒸馏后,添加酵母FC9和FC9DP的酒样香气属性更为丰富,其中花香、果香、木质香气属性突出,此外,FC9DP酒样香气化合物相对含量也最高。对比蒸馏前后的基酒,结果显示,除了酚类化合物及内酯类化合物,大部分香气化合物的相对含量皆在蒸馏后有所增加,其中FC9DP样品的酯类、醇类、酸类及萜烯类化合物增加最为明显。此外,经过蒸馏,基酒去除了生青味和酸味的不良气味特征,香气品质有所改善。该研究可为白兰地酿造工艺的进一步改进提供数据参考。 展开更多
关键词 白兰地 香气化合物 香气 蒸馏 酵母
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中国大陆强震发生前地震活动增强统计特征分析
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作者 呼楠 石富强 +5 位作者 季灵运 刘洁 祁玉萍 王光明 张丽峰 郭蕾 《地震学报》 CSCD 北大核心 2024年第2期226-241,共16页
依据《中国震例》(1966—2017年)对1966年以来中国大陆东部M_(S)>6.0、西部M_(S)>7.0强震前地震活动增强的异常现象特征进行了系统的梳理,并试图总结不同活动构造地块周缘强震前地震活动增强的异常时空演化特征。结果显示:①我国... 依据《中国震例》(1966—2017年)对1966年以来中国大陆东部M_(S)>6.0、西部M_(S)>7.0强震前地震活动增强的异常现象特征进行了系统的梳理,并试图总结不同活动构造地块周缘强震前地震活动增强的异常时空演化特征。结果显示:①我国大陆大震前普遍存在地震活动增强现象,33个震例中有21个震例存在震前地震活动增强异常,占比达64%;②西部地区大多数震例的地震活动增强空间范围表现为中间尺度或构造尺度,且主震震级越大,越有可能出现大范围的地震活动增强;③西部地区出现中强地震活动增强的概率高于东部地区,前兆地震活动增强的平均震级较大,发生大震(M>7.0)的概率增大;④西部地区地震活动增强的时间尺度与震级成正比,而东部地区随着震级增大,更可能出现中期到中短期的地震活动增强异常;⑤多数西部地区震例在震前出现不同时间尺度的地震活动增强叠加的现象;⑥川滇菱形地块的强震前均出现了中长期尺度的地震条带交会现象、不同空间尺度的地震空区以及中短期尺度的中小震活动增强现象,并且对大震地点有较好的指示意义。巴颜喀拉东边界与北、西及南边界大震前存在显著的不同地震活动增强特征,震前未出现中强地震围空的现象。 展开更多
关键词 前兆地震活动增强 统计特征 活动地块 地震前兆模型
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不同栽培模式对草莓果实挥发性香气物质的影响
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作者 庞夫花 王庆莲 +3 位作者 袁华招 蔡伟建 王珑静 赵密珍 《江苏农业学报》 CSCD 北大核心 2024年第2期359-366,共8页
为探明不同栽培模式对草莓果实挥发性香气物质的影响,以红颜(HY)、天使8号(TS8H)为试验材料,设置常规土壤栽培和高架基质栽培2种模式进行栽培试验。果实成熟后,采用搅拌棒吸附萃取(SBSE)结合气相色谱-质谱(GC-MS)联用技术对草莓果实的... 为探明不同栽培模式对草莓果实挥发性香气物质的影响,以红颜(HY)、天使8号(TS8H)为试验材料,设置常规土壤栽培和高架基质栽培2种模式进行栽培试验。果实成熟后,采用搅拌棒吸附萃取(SBSE)结合气相色谱-质谱(GC-MS)联用技术对草莓果实的香气成分进行测定与分析。结果表明:2个草莓品种中共检测出83种、12大类挥发性香气物质。其中,脂肪酯29种、内酯3种、芳香族酯6种、脂肪醇4种、萜烯醇5种、呋喃酮2种、脂肪酮3种、萜烯酮2种、脂肪酸6种、脂肪醛8种、萜烯6种、其他9种。香气组分中,内酯、萜烯醇、脂肪酯相对含量较高。常规土壤栽培处理红颜果实内酯、萜烯醇、脂肪酯相对含量分别为46.77%、21.46%、16.18%,天使8号分别为44.20%、25.71%、19.77%;高架基质栽培处理红颜果实内酯、萜烯醇、脂肪酯相对含量分别为51.17%、24.21%、13.10%,天使8号分别为43.59%、18.11%、16.54%。不同栽培模式下,同一品种中90%以上挥发性香气物质种类一致,主要挥发性香气物质均为γ-癸内酯、γ-十二内酯、反式橙花叔醇;高架基质栽培方式下红颜果实γ-癸内酯和天使8号果实4-甲氧基-2,5-二甲基-3(2H)-呋喃酮(DMMF)的相对含量明显高于常规土壤栽培。综上,高架基质栽培在维持草莓果实主要挥发性香气物质稳定的同时,还可以提升果实的果香、甜香味。 展开更多
关键词 草莓 栽培模式 挥发性香气物质
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猪微小RNA⁃15b前体突变对其生物学加工过程及前体脂肪细胞分化的影响
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作者 龙熙 孙文阳 +4 位作者 潘红梅 张亮 陈四清 涂志 郭宗义 《动物营养学报》 CAS CSCD 北大核心 2024年第3期1892-1903,共12页
基于在猪微小RNA⁃15b(miR⁃15b)前体(pre⁃miR⁃15b)基因位点第58位碱基上发现了1个C>T突变(+58C>T),本试验旨在探究此突变对微小RNA(miRNA)生物学加工过程以及对猪前体脂肪细胞分化的影响。试验通过构建野生型(pmR⁃miR⁃15bW)和突变... 基于在猪微小RNA⁃15b(miR⁃15b)前体(pre⁃miR⁃15b)基因位点第58位碱基上发现了1个C>T突变(+58C>T),本试验旨在探究此突变对微小RNA(miRNA)生物学加工过程以及对猪前体脂肪细胞分化的影响。试验通过构建野生型(pmR⁃miR⁃15bW)和突变型(pmR⁃miR⁃15bM)miR⁃15b过表达载体,分别转染猪原代前体脂肪细胞进行miR⁃15b的过表达试验,然后利用实时荧光定量PCR对比2组细胞pre⁃miR⁃15b和miR⁃15b成熟体的表达量,在体外细胞水平明确+58C>T对miR⁃15b生物学加工过程的影响;随后,诱导转染了不同基因型miR⁃15b过表达载体(pmR⁃miR⁃15bW和pmR⁃miR⁃15bM)的猪前体脂肪细胞成脂分化,利用实时荧光定量PCR对比2组细胞miR⁃15b靶基因叉头框蛋白O1(FOXO1)(可抑制脂肪细胞分化)以及成脂分化相关基因的相对表达量,同时结合油红O染色,明确此突变对猪前体脂肪细胞分化的影响。结果表明:2组细胞miR⁃15b初级转录本(pri⁃miR⁃15b)的表达量没有显著差异(P>0.05),而突变组pre⁃miR⁃15b、miR⁃15b和微小RNA⁃16(miR⁃16)表达量极显著低于野生组(P<0.01);突变组分化前FOXO1相对表达量极显著高于野生组(P<0.01),分化后成脂分化相关基因固醇调节元件结合蛋白-1c(SREBP⁃1c)和过氧化物酶体增殖物激活受体γ(PPARγ)相对表达量极显著低于野生组(P<0.01),脂肪细胞蛋白2(aP2)相对表达量低于野生组(P>0.05);油红O染色结果表明突变组细胞脂滴含量明显低于野生组。综上可知,+58C>T阻碍了pri⁃miR⁃15b到pre⁃miR⁃15b的生物学加工过程,导致miR⁃15b成熟体表达量下降,其靶基因FOXO1相对表达量增高,从而抑制猪前体脂肪细胞分化。 展开更多
关键词 miR⁃15b 突变 前体脂肪细胞 成脂分化
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福红李三个成熟阶段果实营养品质与香气特征分析
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作者 林炎娟 叶新福 +1 位作者 方智振 周丹蓉 《核农学报》 CAS CSCD 北大核心 2024年第4期725-735,共11页
为明确福红李果实成熟期营养品质与香气特征,本研究采用高效液相色谱仪(HPLC)和气相色谱离子迁移谱联用仪(GC-IMS)分析了福红李三个成熟阶段果肉中糖类、有机酸、氨基酸和挥发性成分的组成和含量。结果表明,蔗糖是福红李中的主要糖类,... 为明确福红李果实成熟期营养品质与香气特征,本研究采用高效液相色谱仪(HPLC)和气相色谱离子迁移谱联用仪(GC-IMS)分析了福红李三个成熟阶段果肉中糖类、有机酸、氨基酸和挥发性成分的组成和含量。结果表明,蔗糖是福红李中的主要糖类,占可溶性糖总量高达49.28%,其次是葡萄糖和果糖;苹果酸是主要的有机酸,占有机酸总量高达88.76%,其余有机酸含量均较低;福红李可溶性糖、蔗糖含量和糖酸比随着果实成熟不断积累。福红李中9种非必需氨基酸和7种必需氨基酸含量范围分别为11.58~357.47、55.59~176.63μg·g^(-1),其中,在必需氨基酸中,含量最高的是亮氨酸,其次是赖氨酸和苏氨酸,含量最低的是蛋氨酸,大部分氨基酸主要在成熟后期即花后140 d和花后150 d积累得更多。福红李的主要挥发性成分是醛类、酯类和醇类等,己醛和2-己烯-1-醇峰强度较高,挥发性成分在三个成熟阶段存在差异。本研究初步分析了福红李主要品质成分及成熟阶段变化规律,可为李优质育种提供理论依据。 展开更多
关键词 福红李 成熟阶段 营养品质 香气特征
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不同冷冻-解冻处理方式对猕猴桃品质的影响
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作者 李国龙 汪高玮 +2 位作者 岳田利 王周利 赵旭博 《西北农业学报》 CAS CSCD 北大核心 2024年第1期90-98,共9页
以‘徐香’猕猴桃为原材料,系统探究不同冷冻温度(-20℃、-40℃、-50℃和-80℃)及解冻温度(4℃、15℃、25℃和35℃)组合对猕猴桃主要理化性质及香气成分的影响。结果表明:不同冷冻-解冻处理不会影响猕猴桃的色度;与鲜切猕猴桃一致,4℃/... 以‘徐香’猕猴桃为原材料,系统探究不同冷冻温度(-20℃、-40℃、-50℃和-80℃)及解冻温度(4℃、15℃、25℃和35℃)组合对猕猴桃主要理化性质及香气成分的影响。结果表明:不同冷冻-解冻处理不会影响猕猴桃的色度;与鲜切猕猴桃一致,4℃/-50℃、4℃/-80℃和15℃/-80℃处理条件下猕猴桃的可溶性固形物含量均为16.0oBrix。鲜切猕猴桃的可滴定酸和维生素C含量分别为1.22g/hg和45.30 mg/hg,处理后猕猴桃的可滴定酸含量为1.06~1.33g/hg,维生素C含量降低17.62%~33.89%。不同处理后猕猴桃的多酚类物质损失率为20.44%~31.72%,且不同组合处理之间无显著差异。同时,不同处理对猕猴桃香气成分的种类和含量有一定损失,且较低的冷冻温度和解冻温度有利于香气成分的保持。综合考虑,-50℃/4℃和-80℃/4℃冷冻-解冻组合方式可有效保持猕猴桃的品质,为猕猴桃的贮藏保鲜提供了技术支撑。 展开更多
关键词 冷冻 解冻 猕猴桃 理化性质 香气成分
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2023年汛期气候预测效果评述及先兆信号分析
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作者 支蓉 高辉 孙冷 《气象》 CSCD 北大核心 2024年第3期377-386,共10页
2023年夏季,我国整体表现为高温少雨的特征,旱涝分布不均。夏季台风虽然生成和登陆个数均较常年偏少,但破坏力极强。华北、西北等地阶段性高温事件异常突出,但长江流域的高温干旱强度明显弱于2022年同期。从预测效果来看,2023年汛期预... 2023年夏季,我国整体表现为高温少雨的特征,旱涝分布不均。夏季台风虽然生成和登陆个数均较常年偏少,但破坏力极强。华北、西北等地阶段性高温事件异常突出,但长江流域的高温干旱强度明显弱于2022年同期。从预测效果来看,2023年汛期预测较好把握了我国北方主雨带特征,对松花江流域、嫩江流域、海河流域可能出现的较重汛情做出了准确的预测预警。对夏季热带气旋的生成个数、主要路径、影响区域等的预测与实况较为相符。对夏季全国大部地区气温偏高的特征预测与实况一致,并明确指出,长江流域发生类似2022年夏季大范围持续性高温干旱事件的可能性低。汛期预测的不足之处主要在于对华南等地的降水预测与实况出现较大偏差,对华北、黄淮等地的高温过程极端性估计不足。针对2023年汛期降水的先兆信号及其预报性能进行了回顾和评估,无论是年代际尺度还是年际尺度外强迫信号,均对我国北方主雨带有较好的指示意义。同时,几乎所有先兆信号都对华南降水给出了一致的错误信息,是导致华南降水预报失败的主要原因。 展开更多
关键词 夏季降水 高温热浪 热带气旋 先兆信号
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果香香韵单体在壁式加热卷烟中转移行为分析研究
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作者 李鹏 孙文倩 +5 位作者 何保江 胡安福 席辉 付英杰 汤晓东 尹洁 《中国烟草学报》 CAS CSCD 北大核心 2024年第2期106-114,共9页
为量化表征果香单体香原料在壁式加热卷烟中的调香可用性,基于气相色谱质谱联用技术和嗅觉感官阈值测定,分别研究了果香单体香料在壁式加热卷烟中的转移分布、加香贡献度及逐口释放行为。通过研究发现:1)果香单体香原料的加香贡献度D值... 为量化表征果香单体香原料在壁式加热卷烟中的调香可用性,基于气相色谱质谱联用技术和嗅觉感官阈值测定,分别研究了果香单体香料在壁式加热卷烟中的转移分布、加香贡献度及逐口释放行为。通过研究发现:1)果香单体香原料的加香贡献度D值差距甚大,其中D值最大的γ-辛内酯(12485.0)是苯甲醇(0.1)的10万倍;2)果香单体香料的主流烟气转移率不高,约7%~37%,但高沸点香料损失主要原因是冷凝截留而低沸点香料损失主要原因是抽吸过程中空气散失;3)果香单体香料的逐口释放量随抽吸口序增加有两种趋势,一种呈逐口递减趋势,另一种呈先增后递减趋势。其中庚酸乙酯,辛酸乙酯,癸酸乙酯,乙酰丙酸乙酯和苯乙酸乙酯属递减,肉桂酸乙酯,γ-庚内酯,γ-辛内酯,γ-癸内酯等属先增后减。 展开更多
关键词 壁式加热卷烟 果香香韵单体 调香 转移行为
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基于加热卷烟的国内烟草原料低温热解香气成分差异性分析
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作者 窦玉青 王超 +4 位作者 赵文涛 杨菁 张忠锋 刘天择 刘佩佩 《甘肃农业大学学报》 CAS CSCD 北大核心 2024年第1期203-210,共8页
【目的】明确国内不同类型烟草在加热卷烟体系下的香气种类及释放量的差异。【方法】收集国内10个省份5种烟草类型的71份烟叶样品,利用热裂解-气质联用仪(PY-GC/MC)检测了样品在300℃条件下气溶胶中香气成分含量,【结果】不同类型烟草... 【目的】明确国内不同类型烟草在加热卷烟体系下的香气种类及释放量的差异。【方法】收集国内10个省份5种烟草类型的71份烟叶样品,利用热裂解-气质联用仪(PY-GC/MC)检测了样品在300℃条件下气溶胶中香气成分含量,【结果】不同类型烟草低温热裂解气溶胶中的生物碱种类、含量均有较大差异,晒红烟、晒黄烟、雪茄烟测到5种生物碱,烤烟和香料烟测到3种生物碱,晒红烟热裂解气溶胶烟碱最高,烤烟、晒黄烟、雪茄烟次之,香料烟最低,三组间差异达到显著水平,晒红烟300℃热裂解气溶胶中降烟碱、麦斯明、二烯烟碱、2,3'-联吡啶含量均最高;不同类型烟草低温热解气溶胶中香气物质种类、含量均有较大差异,检测到烤烟气溶胶有16种香气成分,晒黄烟有19种香气成分,晒红烟有18种香气成分,香料烟有13种香气成分,雪茄烟有15种香气成分。【结论】综合分析5种烟草类型热解烟气中共有的香气成分,初步认为,不同类型烟草300℃热解气溶胶中的香气成分种类和含量有较大差异;烤烟、晒黄烟的热解气溶胶中的香气成分含量相近,属于香气成分较高的烟草类型,香料烟组次之,雪茄烟、晒红烟组最低;晒黄烟可能是香气种类丰富且含量较高的一类烟草类型。 展开更多
关键词 加热卷烟 烟草原料 烟草类型 香气成分 差异性
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