Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria invol...Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria involved in effusion fluid infections in paediatrics in order to improve the choice of probabilistic antibiotics therapy. Methods: A cross-sectional, descriptive study was used in children aged 0 to 15 years from 2017 to 2020 at the Charles De Gaulle Pediatric University Hospital Center (CHUP-CDG) in Ouagadougou. Classical bacteriology methods such as macroscopy, Gram staining, identification galleries and antibiotics susceptibility testing were used. Results: Of 231 samples, 64 bacteria were isolated. The most common bacterial strains of pleural fluid were Staphylococcus aureus (25%) and 40% for Enterobacteriaceae. Of the peritoneal fluid, 77% were Enterobacteriaceae with 57% Escherichia coli;and from joint fluid, 33% were S. aureus and 22% for P. aeruginosa. The overall susceptibility profile showed 29% extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL), 10% methicillin-resistant S. aureus (MRSA), and 8% carbapenemases. Conclusion: Bacteriological profile is characterized by ESBL-producing Enterobacteriaceae and MRSA. The most active antibiotics were macrolides, aminoglycosides, and cefoxitin (methicillin) for Gram-positive cocci, carbapenems, and aminoglycosides for Gram-negative bacilli. Then, the monitoring of antibiotics resistance must be permanent.展开更多
Context and Objective: Cervicofacial cellulitis is a lethal infection without treatment. The aim of this study is to establish the bacteriological and antimicrobial susceptibility profile of cervico-facial cellulitis ...Context and Objective: Cervicofacial cellulitis is a lethal infection without treatment. The aim of this study is to establish the bacteriological and antimicrobial susceptibility profile of cervico-facial cellulitis at the Regional Teaching Hospital (RTH) of Ouahigouya, in order to guide practitioners in the development of effective probabilistic antibiotic therapy protocols. Subjects and Methods: This was a transversal descriptive study with prospective data collection from July 1 to December 31, 2021 at the RTH of Ouahigouya. All cases of suppurative cervicofacial cellulitis that had been the subject of pyoculture were retained. Results: A total of 63 patients were chosen including 41 men, with 40.91 years as the average age and the sex ratio was 1.86. In 90.48% of cases, the front door was dental. All patients took antibiotics before their admission. Pus culture was positive in 34/63 subjects (53.97%) and showed monomicrobial infection. The isolates were Gram-negative bacilli for 20.59% and Gram-positive cocci for 79.41%. These isolates were all resistant to certain beta-lactams (such as amoxicillin, amoxicillin + clavulanic acid). However, some isolates were susceptible to cefoxitin, ceftazidime and ceftriaxone. All isolates were sensitive to amikacin for aminoglycosides. As for macrolides, erythromycin had excellent activity (100%) against Gram-positive cocci. Indeed, some isolates were susceptible and others resistant to ciprofloxacin for quinolones. Conclusion: Bacteriological profile and antimicrobial susceptibility knowledge of cervicofacial cellulitis may propose an effective probabilistic antibiotic therapy protocol.展开更多
A study aimed at evaluating the physicochemical and bacteriological quality of surface water was therefore carried out in a few watercourses in the municipality of Mbankomo, located in the Center Region (Cameroon). Th...A study aimed at evaluating the physicochemical and bacteriological quality of surface water was therefore carried out in a few watercourses in the municipality of Mbankomo, located in the Center Region (Cameroon). The physico-chemical parameters were measured according to the standard method. The organic pollution index (OPI) was determined from the contents of nitrates, phosphates, and ammoniacal nitrogen to characterize the level of organic pollution of the water. The microorganisms sought were total flora, indicators of faecal contamination like total coliforms, faecal coliforms, feacal streptococci and Escherichia coli, as well as the species Clostridium perfringens. The organic pollution index indicates a high-level organic pollution level during the small rainy season and high to moderate during the small dry season. High concentrations of the total and fecal coliforms, streptococci fecal, Escherichia coli and Clostridium perfringens respectively of 1712 × 10<sup>5</sup> CFU/100mL;82 × 10<sup>5</sup> CFU/100mL;10 × 10<sup>5</sup> CFU/100mL;27 × 10<sup>5</sup> and 40 × 10<sup>3</sup> CFU/100mL, all higher than WHO standards, indicate that the waterways of Mbankomo are subject to feacal pollution and harbor pathogenic microflora. The cell densities of main bacteria were significantly correlated with main abiotic parameters, such as Escherichia coli, which was significantly correlated with the pH of the water (r = 0.35;p < 0.05). The variation of organic pollution index category means correspond to a high level of organic pollution during the small rainy season and a high to moderate level during the small dry season. The deterioration of the quality of these waters is favored by their proximity to sources of pollution (toilets, plantation), the runoff of the waters contaminated, the use of detergents and wrong maintenance. These waters without any treatment are not recommendable for human consumption according to standards of the World Health Organization.展开更多
A study to evaluate the physico-chemical and bacteriological quality of groundwater was carried out in the commune of Mbankomo(Centre-Cameroon)at 4 wells and 4 springs during the period from March to August.The physic...A study to evaluate the physico-chemical and bacteriological quality of groundwater was carried out in the commune of Mbankomo(Centre-Cameroon)at 4 wells and 4 springs during the period from March to August.The physico-chemical parameters were measured by the standard method.Indicator bacteria of fecal contamination,namely HAB(Heterotrophic Aerobic Bacteria),total and faecal coliforms(Escherichia coli),and faecal streptococci were isolated by the surface spreading technique on PCA(Plate Count Agar),Endo and BEA(Bile Esculin Azide)agar respectively,and sulphite-reducing clostridia by the incorporation method on Tryptone Sulfite Cycloserine(TSC)agar.From a physico-chemical point of view,the groundwater in Mbamkomo is characterized by an average temperature(24.24±0.24°C);it is polluted with organic matter,acid and poorly mineralized.It was noted from a bacteriological point of view that these waters have a high and varied bacterial load with an average value of 100.8 × 10^(5) CFU/100 mL for coliforms,16.3 × 10^(5) CFU/100 mL for fecal streptococci,5.6 × 10^(5) CFU/100 mL for Escherichia coli and 4.8 ×10^(²)CFU/100 mL for Clostridium perfringens.These concentrations are all higher than the WHO(World Health Organization)standards which are set at 0 CFU/100 mL for coliforms,fecal streptococci and Clostridium perfringens and indicate fecal pollution of these waters.Escherichia coli cell densities were significantly correlated with dissolved oxygen in the water(r=0.510;p<0.05).The degradation of the quality of these waters is favored by their proximity to pollution sources,poor maintenance and poor protection of the wells.These waters,without any treatment,are not recommended for human consumption according to the standards of the WHO.展开更多
Introduction: Urinary tract infection is one of the most frequent public health problems in the world and concerns all ages. Objective: To describe the epidemiological, clinical and biological profile of urinary tract...Introduction: Urinary tract infection is one of the most frequent public health problems in the world and concerns all ages. Objective: To describe the epidemiological, clinical and biological profile of urinary tract infection in the internal medicine unit of the Fousseyni Daou hospital in Kayes. Methodology: It was a descriptive and cross-sectional study with retrospective data collection which took place from January 1 to December 31, 2020 at the Internal Medicine Unit of the Fousseyni Daou Hospital in Kayes. It covered all outpatients and hospitalized patients presenting with a urinary tract infection during the study period. Results: We identified 79 cases of urinary tract infection out of 145 requests for ECBU, that is a positivity rate of 53.10% and out of a total of 1883 admissions that is a hospital prevalence of 4.19%. The age groups of 36 to 45 years and 46 to 55 years were more represented in 28 cases (35.44%) and 25 cases (31.64%) with an average age of 45 years ± 10. The female sex was predominant, 42 cases (53.16%) with a sex ratio of 0.88. Housewives were more represented 28 cases (34.44%). The majority of patients resided in rural areas 49 cases (62.03%). The history was dominated by the following pathologies: hypertension 48.10%, diabetes 36.70% and gastropathy 30.37%. The main clinical manifestations were: Urinary burning 67 cases (84.81%), Dysuria 65 cases (82.27%), Pelvic pain 59 cases (74.68%), Fever 57 cases (72.15%). The epidemiological profile of the isolated strains was dominated by enterobacteriaceae, followed by gram-positive cocci and gram-negative bacilli. The main bacteria responsible for urinary tract infections in order of frequency: Escherichia coli (45.56%), Klebsiella pneumoniae (11.39%), Pseudomonas aeruginosa (8.86%), Staphylococcus aureus (7.59%), Streptococcus sp (6.33%), Enterobacter cloacae (3.79%), Acinetobacter sp (3.79%). The study of the susceptibility and resistance profiles of the isolated strains showed significant sensitivity of enterobacteriaceae to colistin and resistance to amoxicillin and to the amoxicillin + clavulanic acid combination. Conclusion: Urinary tract infections are a major public health problem since they are responsible for significant morbidity and mortality and a high cost of care. Knowledge of the epidemiological-clinical and bacteriological profile of these infections is essential for efficient management.展开更多
The surface and bottom waters samples were collected from six locations in Xiamen western sea. The quantified estimation of bacterial production (H-3-thymidine method) and observation of bacterial heterotrophic activi...The surface and bottom waters samples were collected from six locations in Xiamen western sea. The quantified estimation of bacterial production (H-3-thymidine method) and observation of bacterial heterotrophic activity (C-14-glucose method) have been made in order to have a better understanding of the role of marine bacteria and their activities. The results showed that the mean value of bacterial heterotrophic activity was 9 X 10(8) cells/(L. h) in the surface waters and 2.6 X 10(8) cells/(L. h) in the bottom waters. The mean value of bacterial production was 38 X 108 cells/( L. h) in the surface waters and 7.1 X 10(8) cells/(L. h) in the bottom waters. The relationship between bacterial production, heterotrophic activity, PCC and DOC measured during this survey were discussed. The good understanding of the relationship between bacteria activity and total coliform was addressed.展开更多
Objective: To investigate the environmental influences on the bacteriological quality of red and chicken meats on fridges.Methods: The environmental health status was determined by reliable and valid researcher-made c...Objective: To investigate the environmental influences on the bacteriological quality of red and chicken meats on fridges.Methods: The environmental health status was determined by reliable and valid researcher-made checklist.Then 264 samples were gathered in two phases(at the entrance and three months later) and examined for total bacteria count, Escherichia coli,Staphylococcus aureus and Salmonella spp.Results: The result revealed that the mean of total bacteria count, E.coli and S.aureus densities had significant differences in two steps on chicken and the red meat samples(P < 0.05).Among the environmental factors, sanitary status, temperature and personal hygiene had significant effects on total bacteria count and S.aureus densities in chicken samples(P < 0.05), and between wastewater and solid waste disposal with E.coli density in red meat samples(P < 0.05).Conclusions: The results implied that the bacteriological quality of red and chicken meat fluctuates with environmental status(especially temperature, sanitary status and personal hygiene).Regular control, improving of sanitary health, and staff training are necessary for elimination of bacterial contamination.展开更多
Objective:To evaluate the bacteriological safety of sachet water sold in Amassoma,a rural community in Bayelsa State,Nigeria.Methods:Six samples of each of the different sachet drinking water brands were bought at ran...Objective:To evaluate the bacteriological safety of sachet water sold in Amassoma,a rural community in Bayelsa State,Nigeria.Methods:Six samples of each of the different sachet drinking water brands were bought at random from shop shelves,markets and street vendors and were studies for microbial indicators of safety and quality.Bacterial counts Were analyzed by one-way Analysis of Variance（ANOVA） and significance of differences was tested at 5% probability.Results:Minimum and maximum.counts with regard to the sachet water samples investigated were（4.3±1.1）×10<sup>6</sup> CFU mL<sup>-1</sup> and（8.2±1.0）×10<sup>6</sup> CFU mL<sup>-1</sup> for heterotrophic plate counts;（0.9±0.3）×10<sup>6</sup> CFU mL<sup>-1</sup> and（1.2±0.4）×10<sup>6</sup> CFU mL<sup>-1</sup> for aerobic spore-former counts; （1.3±0.5）×10<sup>3</sup> CFU mL<sup>-1</sup> and（2.5±0.8）×10<sup>3</sup> CFU mL<sup>-1</sup> for total coliforms;（1.6±0.9）×10<sup>3</sup> CFU mL<sup>-1</sup> and（9.5±11.2）×10<sup>3</sup> CFU mL<sup>-1</sup> for thermotolerant coliforms.Klebsiella spp but not Escherichia coli was present in all samples of the brands;non-coliform bacteria detected in some samples were Staphylococcus,Pseudomonas and Bacillus species.Conclusions:The brands of sachet water sold（at the time of this study） in Amassoma did not meet the minimum acceptable standard for microbiologically safe drinking water as recommended by the World Health Organization.展开更多
Majority of the population in the Bambui student residential area depend on wells as their source of water supply. Due to increasing complaints from students after using this well water sources, this study was carried...Majority of the population in the Bambui student residential area depend on wells as their source of water supply. Due to increasing complaints from students after using this well water sources, this study was carried out to examine the microbial quality of well water sources in the Bambui student residential area, Bamenda, Cameroon as a way of safe guarding public health against water borne diseases. Water samples from a total of fifteen wells, covered and uncovered were assessed for their bacteriological quality using the multiple tube fermentation technique to determine the coliform count using the most probable number method. Positive tubes of the presumptive test were further cultured on appropriate solid media. The organisms isolated were further characterized using standard procedures. The result of the study revealed that most of the wells were grossly contaminated with bacteria pathogens such as Klebsiella species (95%), Escherichia coli (52%) and Salmonella typhi (32.5%). Comparatively, the uncovered wells were more contaminated with bacteria pathogens than the covered well. All the water samples except three exceeded the standard limit of the most probable number (MPN) per 100 ml set for untreated drinking water by the World Health Organization. This result highlight the fact that most well water sources in the Bambui student residential area metropolis are not safe microbiologically for drinking without additional treatment such as boiling or disinfection and this could lead to outbreak of water borne diseases. Good and proper environmental and personal hygiene must be maintained especially by the users of these wells to prevent their contamination with bacterial pathogens. Wells should also be properly constructed.展开更多
Due to lack of quantitative standard reference material, bacteriological monitoring has the characteristics of strong professionality and heavy workload. It is necessary to conduct quality control in sampling preparat...Due to lack of quantitative standard reference material, bacteriological monitoring has the characteristics of strong professionality and heavy workload. It is necessary to conduct quality control in sampling preparation, sample collection technology, sample preservation and transportation, laboratory equipment, supply quality and monitoring personnel requirements in order to improve the quality of monitoring data and ensure the representativeness, accuracy, precision, comparability and integrity of the monitoring results.展开更多
The physico-chemical and bacteriological parameters of the water in Taladanda canal and associated water-borne diseases,from which the dwellers have suffered,were studied,by using statistical method.Overuse and the ad...The physico-chemical and bacteriological parameters of the water in Taladanda canal and associated water-borne diseases,from which the dwellers have suffered,were studied,by using statistical method.Overuse and the addition of the wastes with sources from urbane industrial sectors,as well as the decrease in water level have caused the canal water quality declined drastically and subsequently led to extensive eutrophication and bacterial contamination.According to the water sample analytical results,the water is lightly acidic with the pH value of 4.5~6.7.The measured indexes,such as total dissolved solid(TDS),electrical conductivity(EC),total suspended solid(TSS),Mn,Zn,Al,Fe,Cu,Cr,and Hg etc,mostly have very high concentrations which are higher than permissible limit,indicating that the canal water is completely unsuitable for human consumption.Furthermore,the biological analysis shows that the total coliform(TC)is in the range of 45.9~30.2 in per 100 mL water in April,30.5~25.3/100 mL in July and 52.9~35.4/100 mL in December,respectively.Similarly,fecal coliform(FC)ranges from 12.8 to 10.1,10.5~7.5 and 13.1~6.4 per 100 mL water in the months of April,July and December respectively.As a result,people who use the water have suffered from different water-borne diseases.On the basis of disease data derived from hospital observations in a period of three years,there had been 4284 people affected by different waterborne diseases from 2016 to 2018.展开更多
The study aimed to assess the physical, chemical and bacteriological quality of drinking water and its potential health effect on study area population. Physical, chemical and bacteriological parameters in six station...The study aimed to assess the physical, chemical and bacteriological quality of drinking water and its potential health effect on study area population. Physical, chemical and bacteriological parameters in six stations of four water springs of Hajigak iron ore mine were studied. These four springs are the predominant source of water in this area. The inhabitant’s villages around the area of interest use these waters 12 months per year without any treatment. Whereas this mine consists of 66% of iron, 6 PPM gold, 26 PPM silver, thorium and some poly metals that directly affect the quality of water. The quality of these water sources has not been investigated and analyzed before for drinking purpose. Therefore studies on spring water quality required analysis for chemical, physical and bacteriological parameters. Chemical and physical analyses revealed that these spring waters are toxic free and the area has very fresh and good quality water based on the guidelines of World Health Organization, national standard of most Asian countries and standard values for Afghanistan. However, the bacteriological analysis detected the E. coli, fecal coli form and total coli form bacteria in water samples that may cause some diseases and health problems.展开更多
Introduction: Acute appendicitis is the most common cause of abdominal pain requiring surgery in children. In most instances the infecting organisms are normal inhabitants of the lumen of appendix. Surgery allows easy...Introduction: Acute appendicitis is the most common cause of abdominal pain requiring surgery in children. In most instances the infecting organisms are normal inhabitants of the lumen of appendix. Surgery allows easy microbiological sampling. Aspiration of fluid or pus in a syringe is preferred. Swabs are less suitable and only to be used when sampling with a syringe is not feasible. Antimicrobial susceptibility testing of the isolated bacteria and particularly of the anaer- obes can be important to adjust therapy in case of the presence of multi resistant bacteria. Objective: The aim of the study was to determine the bacteriological profile of acute appendicitis in children. Materials and Methods: Study design is a prospective descriptive study including children hospitalized for acute appendicitis. Tissue samples (a speci- men of the appendix), peritoneal fluid swab from the appendicial fossa and the peritoneal exudates (if exists) obtained at surgery from 54 children with suspected acute appendicitis operated at the pediatric surgery unit at the Maternity and Child Teaching Hospital in Al-Qadisiya province from the period 1st of June 2007 to the end of May 2011, were exam- ined histologically and by culture for aerobic and anaerobic bacteria. Results: Out of these, 39 boys (72.2%) and 15 (27.7% girls). Their age ranged between (1.8 - 13) years, with a mean of 6.9 years. Of the all patients studied 34 pre- sented with suppurative and phlegmnous appendicitis, 8 with gangrenous appendicitis, 6 with septic complications of appendicitis and 6 had normal appendices. Only 80 of the 108 swabs taken yielded a positive culture (74.07%). Con- clusion: Although in our study no antibiotic regime was changed on the basis of a positive culture swab and the perito- neal culture swabs do not improve immediate postoperative therapy based on surgical impression and rapid histological reporting, however, the routine use of peritoneal culture swabs may be of value in identifying patients requiring outpa- tient follow-up.展开更多
The bacteriological quality of swimming pool water in Port Harcourt Metropolis was investigated. Ten (10) swimming pools were examined for microbial quality. Out of the 10 swimming pools, 4 (2, 4, 7 and 9) had bacteri...The bacteriological quality of swimming pool water in Port Harcourt Metropolis was investigated. Ten (10) swimming pools were examined for microbial quality. Out of the 10 swimming pools, 4 (2, 4, 7 and 9) had bacterial isolates of 40 (100%). Swimming pools 2, 4, 7 and 9 had the bacterial isolates of 10 (25%), 8 (20%), 10 (25%) and 12 (30%), respectively. Of the forty (40) bacterial isolates identified, which represented 100 percent, 22 (55%) were identified as Staphylococcus epidermidis, 10 (25%) Bacillus cereus, 6 (16%) Micrococcus and 2 (5%) Staphylococcus aureus. Among the four swimming pools, 2 and 9 did not have the isolates of Staphylococcus aureus and Micrococcus, respectively. Based on the World Health Organisation (WHO) standard for recreational waters, the absence of coliform and fecal coliform bacteria (E. coli) revealed that the ten (10) swimming pools used for this study are considered to be within the acceptable limits for certifying microbiological water quality. However, there is a need for care and continuous maintenance of the swimming pools.展开更多
In this work, the authors made aquatic filters according to the formulation “clay stabilized at 4% of cement mixed with 4% of kambala sawdust and 10% of white sand” then heated to 1050°C to decontaminate the wa...In this work, the authors made aquatic filters according to the formulation “clay stabilized at 4% of cement mixed with 4% of kambala sawdust and 10% of white sand” then heated to 1050°C to decontaminate the waters of gutters and wells. The authors carried out geotechnical, geochemical, thermal, infrared spectroscopy, and scanning electron microscopy that analyzed the clay material. Geotechnical analyzes have shown that this material is made up of 22% thin sand, 22% of silt, and 56% of clay with 26 plasticity index. The geochemical analysis showed the presence of trace elements shared out as follows: 3% of alkaline metals, 24% of alkaline earth metals, 46% of transition metals, 10% of metal, 16% of lanthanides, 1% of actinides. The most abundant trace elements are barium (19%), vanadium (12%), chromium (11%) and zinc (9%). The thermal and microscopic analyzes revealed the kaolinitic nature of materials. The chemical depollution studies have shown elimination yields of 50% - 52.38% of sulphates;77.33% - 85.19% of phosphates;34.85% - 88.49% nitrates;91.3 - 100 of sulphides;The removal of bacteriological pollution are 92.8% - 98% of total germs;94% - 97% of total coliform and 95% - 98% of <em>E. coli</em>.展开更多
The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its wa...The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its watershed is estimated at about 62 Km2. The objective of the present study is on the one hand to evaluate the current state of the physico-chemical and bacteriological quality of the water of Gounti Yéna basin, and on the other hand to define the risks of water pollution and its origin in the face of the phenomenon of rising water table of this basin. In order to carry out this work, we proceeded to a study of the evolution of the physicochemical and bacteriological parameters of the water of the basin of Gounti Yéna during the period going from November 2020 to October 2021, at the level of four points of sampling chosen from the upstream to the downstream of the basin. The results obtained showed that the physico-chemical quality of the water in Gounti Yéna basin is influenced by the lithology of the watershed, climatic factors (rainfall and temperature) and anthropic actions. This study also highlighted the presence of fecal contamination in the water of the Gounti Yéna basin, with a greater degree of emphasis during the rainy season. This contamination remains a concern and may constitute a health risk.展开更多
The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization ...The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization (WHO). In rural and peri-urban areas of Benin where public water supply systems are inadequate or almost non-existent, the population consumes water of various sources of unknown qualities. A total of 67 water samples were analyzed during the rainy season (July 2017) and in the dry season (January 2018) for certain physical, chemical and bacteriological parameters using the standard methods. The results of the analyses reveal that the physicochemical characteristics of the water used for consumption in S?-Ava comply with the drinking water standards of the World Health Organization and those in force in Benin except for the percentages of the following parameters: pH (41.80%);turbidity (25.37%);the color (16.42);ammonium (17.91%);iron (40.30%);Nitrites (4.48%);Residual chlorine (91.05). Bacteriologically, the analyses showed a high total aerobic mesophilic flora contamination, faecal coliforms, E. coli, faecal enterococci respectively in 89.55%, 82.09%, 50.75% and 70.15% of the analyzed water samples. The ratio of faecal coliforms to faecal enterococci indicated that the origin of faecal contamination was human in 59.7% of the samples and animal in 40.3% of the samples. The adoption of hygiene measures at the water point, during the transport and storage of water, including the treatment by chlorination of drinking water at the family level was recommended for the population concerned and household awareness on the adoption of basic hygiene and sanitation measures have been recommended for hygiene and sanitation services.展开更多
To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in eart...To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.展开更多
Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence o...Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence of resistant strains of bacteria is ever-present. Hence, a sensitivity directed therapy is paramount for the successful eradication of organisms with minimal risk of development of antibiotic resistance. Aim: The aim is to identify the common bacteria that cause SSI in orthopaedic implant surgeries in our hospital. Method: This is a prospective longitudinal study that includes all orthopaedic surgeries involving the use of implants within one year. Patients that had major orthopaedic surgeries involving implant were followed up and their wounds inspected for signs of SSI on postoperative days 3, 7, 14, 42 and 90. Wound swab was taken for microscopy, culture and sensitivity analysis from those who had wound infection, based on the CDC guidelines. Results: One-hundred and sixteen patients met our inclusion criteria and were included in the analysis. There were 62 males and 54 females. The mean age of the participant was 39.62 years (SD = 15.02 years). Fracture fixation with plates and screws was the most common implant surgery done. The incidence of SSI was 2.6%, and Escherichia coli was the most common isolated pathogen. All the SSIs were superficial incisional type, and the infection was monomicrobial in 67% of cases and polymicrobial in 33%. All of the isolated pathogens were sensitive to Imipenem and Gentamycin. Conclusion: Superficial incisional SSI is the most common type of SSI in this study. Escherichia coli is the most frequent pathogen in SSI affecting implant surgeries in our hospital. Gentamycin and Imipenem should be used for the prophylaxis of SSI in our environment.展开更多
The drinking water for all the inhabitants of Tangier is still an aim far from being achieved. Therefore, all rural areas and some urban areas feed water wells and springs whose composition and safety are unknown. In ...The drinking water for all the inhabitants of Tangier is still an aim far from being achieved. Therefore, all rural areas and some urban areas feed water wells and springs whose composition and safety are unknown. In the absence of data on water and ignorance of consumers towards the risk of poisoning and waterborne diseases transmitted by these waters, the authors conducted a study to determine the composition, physico-chemical quality and bacteriological these waters. This study is to provide a basic physico-chemical and bacteriological data from these waters in some urban areas of old or missing infrastructure in some sites in the province of Tangier. Such as Ziatine sites, Gzenaya, Rmilate, Malabata ＋ Mnar, Mghoura ＋ Aouama and Downtown. Analyzes are performed according to AFNOR methods （AFNOR： French Standards Association）. The results indicate that these waters are generally mild and are experiencing significant geochemical imbalance. Moreover, these waters also have a significant organic and bacteriological pollution. It is concluded that the waters of these sites are of poor quality, unfit for human consumption.展开更多
文摘Introduction: Microbiology of effusion fluids in children in Burkina Faso is characterized by the scarcity of data. This work aimed to study the bacteriological and antibiotics susceptibility profile of bacteria involved in effusion fluid infections in paediatrics in order to improve the choice of probabilistic antibiotics therapy. Methods: A cross-sectional, descriptive study was used in children aged 0 to 15 years from 2017 to 2020 at the Charles De Gaulle Pediatric University Hospital Center (CHUP-CDG) in Ouagadougou. Classical bacteriology methods such as macroscopy, Gram staining, identification galleries and antibiotics susceptibility testing were used. Results: Of 231 samples, 64 bacteria were isolated. The most common bacterial strains of pleural fluid were Staphylococcus aureus (25%) and 40% for Enterobacteriaceae. Of the peritoneal fluid, 77% were Enterobacteriaceae with 57% Escherichia coli;and from joint fluid, 33% were S. aureus and 22% for P. aeruginosa. The overall susceptibility profile showed 29% extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL), 10% methicillin-resistant S. aureus (MRSA), and 8% carbapenemases. Conclusion: Bacteriological profile is characterized by ESBL-producing Enterobacteriaceae and MRSA. The most active antibiotics were macrolides, aminoglycosides, and cefoxitin (methicillin) for Gram-positive cocci, carbapenems, and aminoglycosides for Gram-negative bacilli. Then, the monitoring of antibiotics resistance must be permanent.
文摘Context and Objective: Cervicofacial cellulitis is a lethal infection without treatment. The aim of this study is to establish the bacteriological and antimicrobial susceptibility profile of cervico-facial cellulitis at the Regional Teaching Hospital (RTH) of Ouahigouya, in order to guide practitioners in the development of effective probabilistic antibiotic therapy protocols. Subjects and Methods: This was a transversal descriptive study with prospective data collection from July 1 to December 31, 2021 at the RTH of Ouahigouya. All cases of suppurative cervicofacial cellulitis that had been the subject of pyoculture were retained. Results: A total of 63 patients were chosen including 41 men, with 40.91 years as the average age and the sex ratio was 1.86. In 90.48% of cases, the front door was dental. All patients took antibiotics before their admission. Pus culture was positive in 34/63 subjects (53.97%) and showed monomicrobial infection. The isolates were Gram-negative bacilli for 20.59% and Gram-positive cocci for 79.41%. These isolates were all resistant to certain beta-lactams (such as amoxicillin, amoxicillin + clavulanic acid). However, some isolates were susceptible to cefoxitin, ceftazidime and ceftriaxone. All isolates were sensitive to amikacin for aminoglycosides. As for macrolides, erythromycin had excellent activity (100%) against Gram-positive cocci. Indeed, some isolates were susceptible and others resistant to ciprofloxacin for quinolones. Conclusion: Bacteriological profile and antimicrobial susceptibility knowledge of cervicofacial cellulitis may propose an effective probabilistic antibiotic therapy protocol.
文摘A study aimed at evaluating the physicochemical and bacteriological quality of surface water was therefore carried out in a few watercourses in the municipality of Mbankomo, located in the Center Region (Cameroon). The physico-chemical parameters were measured according to the standard method. The organic pollution index (OPI) was determined from the contents of nitrates, phosphates, and ammoniacal nitrogen to characterize the level of organic pollution of the water. The microorganisms sought were total flora, indicators of faecal contamination like total coliforms, faecal coliforms, feacal streptococci and Escherichia coli, as well as the species Clostridium perfringens. The organic pollution index indicates a high-level organic pollution level during the small rainy season and high to moderate during the small dry season. High concentrations of the total and fecal coliforms, streptococci fecal, Escherichia coli and Clostridium perfringens respectively of 1712 × 10<sup>5</sup> CFU/100mL;82 × 10<sup>5</sup> CFU/100mL;10 × 10<sup>5</sup> CFU/100mL;27 × 10<sup>5</sup> and 40 × 10<sup>3</sup> CFU/100mL, all higher than WHO standards, indicate that the waterways of Mbankomo are subject to feacal pollution and harbor pathogenic microflora. The cell densities of main bacteria were significantly correlated with main abiotic parameters, such as Escherichia coli, which was significantly correlated with the pH of the water (r = 0.35;p < 0.05). The variation of organic pollution index category means correspond to a high level of organic pollution during the small rainy season and a high to moderate level during the small dry season. The deterioration of the quality of these waters is favored by their proximity to sources of pollution (toilets, plantation), the runoff of the waters contaminated, the use of detergents and wrong maintenance. These waters without any treatment are not recommendable for human consumption according to standards of the World Health Organization.
文摘A study to evaluate the physico-chemical and bacteriological quality of groundwater was carried out in the commune of Mbankomo(Centre-Cameroon)at 4 wells and 4 springs during the period from March to August.The physico-chemical parameters were measured by the standard method.Indicator bacteria of fecal contamination,namely HAB(Heterotrophic Aerobic Bacteria),total and faecal coliforms(Escherichia coli),and faecal streptococci were isolated by the surface spreading technique on PCA(Plate Count Agar),Endo and BEA(Bile Esculin Azide)agar respectively,and sulphite-reducing clostridia by the incorporation method on Tryptone Sulfite Cycloserine(TSC)agar.From a physico-chemical point of view,the groundwater in Mbamkomo is characterized by an average temperature(24.24±0.24°C);it is polluted with organic matter,acid and poorly mineralized.It was noted from a bacteriological point of view that these waters have a high and varied bacterial load with an average value of 100.8 × 10^(5) CFU/100 mL for coliforms,16.3 × 10^(5) CFU/100 mL for fecal streptococci,5.6 × 10^(5) CFU/100 mL for Escherichia coli and 4.8 ×10^(²)CFU/100 mL for Clostridium perfringens.These concentrations are all higher than the WHO(World Health Organization)standards which are set at 0 CFU/100 mL for coliforms,fecal streptococci and Clostridium perfringens and indicate fecal pollution of these waters.Escherichia coli cell densities were significantly correlated with dissolved oxygen in the water(r=0.510;p<0.05).The degradation of the quality of these waters is favored by their proximity to pollution sources,poor maintenance and poor protection of the wells.These waters,without any treatment,are not recommended for human consumption according to the standards of the WHO.
文摘Introduction: Urinary tract infection is one of the most frequent public health problems in the world and concerns all ages. Objective: To describe the epidemiological, clinical and biological profile of urinary tract infection in the internal medicine unit of the Fousseyni Daou hospital in Kayes. Methodology: It was a descriptive and cross-sectional study with retrospective data collection which took place from January 1 to December 31, 2020 at the Internal Medicine Unit of the Fousseyni Daou Hospital in Kayes. It covered all outpatients and hospitalized patients presenting with a urinary tract infection during the study period. Results: We identified 79 cases of urinary tract infection out of 145 requests for ECBU, that is a positivity rate of 53.10% and out of a total of 1883 admissions that is a hospital prevalence of 4.19%. The age groups of 36 to 45 years and 46 to 55 years were more represented in 28 cases (35.44%) and 25 cases (31.64%) with an average age of 45 years ± 10. The female sex was predominant, 42 cases (53.16%) with a sex ratio of 0.88. Housewives were more represented 28 cases (34.44%). The majority of patients resided in rural areas 49 cases (62.03%). The history was dominated by the following pathologies: hypertension 48.10%, diabetes 36.70% and gastropathy 30.37%. The main clinical manifestations were: Urinary burning 67 cases (84.81%), Dysuria 65 cases (82.27%), Pelvic pain 59 cases (74.68%), Fever 57 cases (72.15%). The epidemiological profile of the isolated strains was dominated by enterobacteriaceae, followed by gram-positive cocci and gram-negative bacilli. The main bacteria responsible for urinary tract infections in order of frequency: Escherichia coli (45.56%), Klebsiella pneumoniae (11.39%), Pseudomonas aeruginosa (8.86%), Staphylococcus aureus (7.59%), Streptococcus sp (6.33%), Enterobacter cloacae (3.79%), Acinetobacter sp (3.79%). The study of the susceptibility and resistance profiles of the isolated strains showed significant sensitivity of enterobacteriaceae to colistin and resistance to amoxicillin and to the amoxicillin + clavulanic acid combination. Conclusion: Urinary tract infections are a major public health problem since they are responsible for significant morbidity and mortality and a high cost of care. Knowledge of the epidemiological-clinical and bacteriological profile of these infections is essential for efficient management.
基金National Natural Science Foundation of China under contract No.30070175 and No. 40206015.
文摘The surface and bottom waters samples were collected from six locations in Xiamen western sea. The quantified estimation of bacterial production (H-3-thymidine method) and observation of bacterial heterotrophic activity (C-14-glucose method) have been made in order to have a better understanding of the role of marine bacteria and their activities. The results showed that the mean value of bacterial heterotrophic activity was 9 X 10(8) cells/(L. h) in the surface waters and 2.6 X 10(8) cells/(L. h) in the bottom waters. The mean value of bacterial production was 38 X 108 cells/( L. h) in the surface waters and 7.1 X 10(8) cells/(L. h) in the bottom waters. The relationship between bacterial production, heterotrophic activity, PCC and DOC measured during this survey were discussed. The good understanding of the relationship between bacteria activity and total coliform was addressed.
基金Supported by the Health Research Center and Faculty of Health of Baqiyatallah University of Medical Science(Grant No.BG52142)
文摘Objective: To investigate the environmental influences on the bacteriological quality of red and chicken meats on fridges.Methods: The environmental health status was determined by reliable and valid researcher-made checklist.Then 264 samples were gathered in two phases(at the entrance and three months later) and examined for total bacteria count, Escherichia coli,Staphylococcus aureus and Salmonella spp.Results: The result revealed that the mean of total bacteria count, E.coli and S.aureus densities had significant differences in two steps on chicken and the red meat samples(P < 0.05).Among the environmental factors, sanitary status, temperature and personal hygiene had significant effects on total bacteria count and S.aureus densities in chicken samples(P < 0.05), and between wastewater and solid waste disposal with E.coli density in red meat samples(P < 0.05).Conclusions: The results implied that the bacteriological quality of red and chicken meat fluctuates with environmental status(especially temperature, sanitary status and personal hygiene).Regular control, improving of sanitary health, and staff training are necessary for elimination of bacterial contamination.
文摘Objective:To evaluate the bacteriological safety of sachet water sold in Amassoma,a rural community in Bayelsa State,Nigeria.Methods:Six samples of each of the different sachet drinking water brands were bought at random from shop shelves,markets and street vendors and were studies for microbial indicators of safety and quality.Bacterial counts Were analyzed by one-way Analysis of Variance（ANOVA） and significance of differences was tested at 5% probability.Results:Minimum and maximum.counts with regard to the sachet water samples investigated were（4.3±1.1）×10<sup>6</sup> CFU mL<sup>-1</sup> and（8.2±1.0）×10<sup>6</sup> CFU mL<sup>-1</sup> for heterotrophic plate counts;（0.9±0.3）×10<sup>6</sup> CFU mL<sup>-1</sup> and（1.2±0.4）×10<sup>6</sup> CFU mL<sup>-1</sup> for aerobic spore-former counts; （1.3±0.5）×10<sup>3</sup> CFU mL<sup>-1</sup> and（2.5±0.8）×10<sup>3</sup> CFU mL<sup>-1</sup> for total coliforms;（1.6±0.9）×10<sup>3</sup> CFU mL<sup>-1</sup> and（9.5±11.2）×10<sup>3</sup> CFU mL<sup>-1</sup> for thermotolerant coliforms.Klebsiella spp but not Escherichia coli was present in all samples of the brands;non-coliform bacteria detected in some samples were Staphylococcus,Pseudomonas and Bacillus species.Conclusions:The brands of sachet water sold（at the time of this study） in Amassoma did not meet the minimum acceptable standard for microbiologically safe drinking water as recommended by the World Health Organization.
文摘Majority of the population in the Bambui student residential area depend on wells as their source of water supply. Due to increasing complaints from students after using this well water sources, this study was carried out to examine the microbial quality of well water sources in the Bambui student residential area, Bamenda, Cameroon as a way of safe guarding public health against water borne diseases. Water samples from a total of fifteen wells, covered and uncovered were assessed for their bacteriological quality using the multiple tube fermentation technique to determine the coliform count using the most probable number method. Positive tubes of the presumptive test were further cultured on appropriate solid media. The organisms isolated were further characterized using standard procedures. The result of the study revealed that most of the wells were grossly contaminated with bacteria pathogens such as Klebsiella species (95%), Escherichia coli (52%) and Salmonella typhi (32.5%). Comparatively, the uncovered wells were more contaminated with bacteria pathogens than the covered well. All the water samples except three exceeded the standard limit of the most probable number (MPN) per 100 ml set for untreated drinking water by the World Health Organization. This result highlight the fact that most well water sources in the Bambui student residential area metropolis are not safe microbiologically for drinking without additional treatment such as boiling or disinfection and this could lead to outbreak of water borne diseases. Good and proper environmental and personal hygiene must be maintained especially by the users of these wells to prevent their contamination with bacterial pathogens. Wells should also be properly constructed.
文摘Due to lack of quantitative standard reference material, bacteriological monitoring has the characteristics of strong professionality and heavy workload. It is necessary to conduct quality control in sampling preparation, sample collection technology, sample preservation and transportation, laboratory equipment, supply quality and monitoring personnel requirements in order to improve the quality of monitoring data and ensure the representativeness, accuracy, precision, comparability and integrity of the monitoring results.
文摘The physico-chemical and bacteriological parameters of the water in Taladanda canal and associated water-borne diseases,from which the dwellers have suffered,were studied,by using statistical method.Overuse and the addition of the wastes with sources from urbane industrial sectors,as well as the decrease in water level have caused the canal water quality declined drastically and subsequently led to extensive eutrophication and bacterial contamination.According to the water sample analytical results,the water is lightly acidic with the pH value of 4.5~6.7.The measured indexes,such as total dissolved solid(TDS),electrical conductivity(EC),total suspended solid(TSS),Mn,Zn,Al,Fe,Cu,Cr,and Hg etc,mostly have very high concentrations which are higher than permissible limit,indicating that the canal water is completely unsuitable for human consumption.Furthermore,the biological analysis shows that the total coliform(TC)is in the range of 45.9~30.2 in per 100 mL water in April,30.5~25.3/100 mL in July and 52.9~35.4/100 mL in December,respectively.Similarly,fecal coliform(FC)ranges from 12.8 to 10.1,10.5~7.5 and 13.1~6.4 per 100 mL water in the months of April,July and December respectively.As a result,people who use the water have suffered from different water-borne diseases.On the basis of disease data derived from hospital observations in a period of three years,there had been 4284 people affected by different waterborne diseases from 2016 to 2018.
文摘The study aimed to assess the physical, chemical and bacteriological quality of drinking water and its potential health effect on study area population. Physical, chemical and bacteriological parameters in six stations of four water springs of Hajigak iron ore mine were studied. These four springs are the predominant source of water in this area. The inhabitant’s villages around the area of interest use these waters 12 months per year without any treatment. Whereas this mine consists of 66% of iron, 6 PPM gold, 26 PPM silver, thorium and some poly metals that directly affect the quality of water. The quality of these water sources has not been investigated and analyzed before for drinking purpose. Therefore studies on spring water quality required analysis for chemical, physical and bacteriological parameters. Chemical and physical analyses revealed that these spring waters are toxic free and the area has very fresh and good quality water based on the guidelines of World Health Organization, national standard of most Asian countries and standard values for Afghanistan. However, the bacteriological analysis detected the E. coli, fecal coli form and total coli form bacteria in water samples that may cause some diseases and health problems.
文摘Introduction: Acute appendicitis is the most common cause of abdominal pain requiring surgery in children. In most instances the infecting organisms are normal inhabitants of the lumen of appendix. Surgery allows easy microbiological sampling. Aspiration of fluid or pus in a syringe is preferred. Swabs are less suitable and only to be used when sampling with a syringe is not feasible. Antimicrobial susceptibility testing of the isolated bacteria and particularly of the anaer- obes can be important to adjust therapy in case of the presence of multi resistant bacteria. Objective: The aim of the study was to determine the bacteriological profile of acute appendicitis in children. Materials and Methods: Study design is a prospective descriptive study including children hospitalized for acute appendicitis. Tissue samples (a speci- men of the appendix), peritoneal fluid swab from the appendicial fossa and the peritoneal exudates (if exists) obtained at surgery from 54 children with suspected acute appendicitis operated at the pediatric surgery unit at the Maternity and Child Teaching Hospital in Al-Qadisiya province from the period 1st of June 2007 to the end of May 2011, were exam- ined histologically and by culture for aerobic and anaerobic bacteria. Results: Out of these, 39 boys (72.2%) and 15 (27.7% girls). Their age ranged between (1.8 - 13) years, with a mean of 6.9 years. Of the all patients studied 34 pre- sented with suppurative and phlegmnous appendicitis, 8 with gangrenous appendicitis, 6 with septic complications of appendicitis and 6 had normal appendices. Only 80 of the 108 swabs taken yielded a positive culture (74.07%). Con- clusion: Although in our study no antibiotic regime was changed on the basis of a positive culture swab and the perito- neal culture swabs do not improve immediate postoperative therapy based on surgical impression and rapid histological reporting, however, the routine use of peritoneal culture swabs may be of value in identifying patients requiring outpa- tient follow-up.
文摘The bacteriological quality of swimming pool water in Port Harcourt Metropolis was investigated. Ten (10) swimming pools were examined for microbial quality. Out of the 10 swimming pools, 4 (2, 4, 7 and 9) had bacterial isolates of 40 (100%). Swimming pools 2, 4, 7 and 9 had the bacterial isolates of 10 (25%), 8 (20%), 10 (25%) and 12 (30%), respectively. Of the forty (40) bacterial isolates identified, which represented 100 percent, 22 (55%) were identified as Staphylococcus epidermidis, 10 (25%) Bacillus cereus, 6 (16%) Micrococcus and 2 (5%) Staphylococcus aureus. Among the four swimming pools, 2 and 9 did not have the isolates of Staphylococcus aureus and Micrococcus, respectively. Based on the World Health Organisation (WHO) standard for recreational waters, the absence of coliform and fecal coliform bacteria (E. coli) revealed that the ten (10) swimming pools used for this study are considered to be within the acceptable limits for certifying microbiological water quality. However, there is a need for care and continuous maintenance of the swimming pools.
文摘In this work, the authors made aquatic filters according to the formulation “clay stabilized at 4% of cement mixed with 4% of kambala sawdust and 10% of white sand” then heated to 1050°C to decontaminate the waters of gutters and wells. The authors carried out geotechnical, geochemical, thermal, infrared spectroscopy, and scanning electron microscopy that analyzed the clay material. Geotechnical analyzes have shown that this material is made up of 22% thin sand, 22% of silt, and 56% of clay with 26 plasticity index. The geochemical analysis showed the presence of trace elements shared out as follows: 3% of alkaline metals, 24% of alkaline earth metals, 46% of transition metals, 10% of metal, 16% of lanthanides, 1% of actinides. The most abundant trace elements are barium (19%), vanadium (12%), chromium (11%) and zinc (9%). The thermal and microscopic analyzes revealed the kaolinitic nature of materials. The chemical depollution studies have shown elimination yields of 50% - 52.38% of sulphates;77.33% - 85.19% of phosphates;34.85% - 88.49% nitrates;91.3 - 100 of sulphides;The removal of bacteriological pollution are 92.8% - 98% of total germs;94% - 97% of total coliform and 95% - 98% of <em>E. coli</em>.
文摘The Gounti Yéna valley, the subject of this study, is a tributary of the Niger River, it is the main watercourse that divides the left bank of the city of Niamey into two unequal parts. The surface area of its watershed is estimated at about 62 Km2. The objective of the present study is on the one hand to evaluate the current state of the physico-chemical and bacteriological quality of the water of Gounti Yéna basin, and on the other hand to define the risks of water pollution and its origin in the face of the phenomenon of rising water table of this basin. In order to carry out this work, we proceeded to a study of the evolution of the physicochemical and bacteriological parameters of the water of the basin of Gounti Yéna during the period going from November 2020 to October 2021, at the level of four points of sampling chosen from the upstream to the downstream of the basin. The results obtained showed that the physico-chemical quality of the water in Gounti Yéna basin is influenced by the lithology of the watershed, climatic factors (rainfall and temperature) and anthropic actions. This study also highlighted the presence of fecal contamination in the water of the Gounti Yéna basin, with a greater degree of emphasis during the rainy season. This contamination remains a concern and may constitute a health risk.
基金the International Foundation for Science(IFS)for contributing to this work.
文摘The present study assesses the physicochemical and bacteriological quality of the drinking water used by the population of S?-Ava based on the Beninese standards and those established by the World Health Organization (WHO). In rural and peri-urban areas of Benin where public water supply systems are inadequate or almost non-existent, the population consumes water of various sources of unknown qualities. A total of 67 water samples were analyzed during the rainy season (July 2017) and in the dry season (January 2018) for certain physical, chemical and bacteriological parameters using the standard methods. The results of the analyses reveal that the physicochemical characteristics of the water used for consumption in S?-Ava comply with the drinking water standards of the World Health Organization and those in force in Benin except for the percentages of the following parameters: pH (41.80%);turbidity (25.37%);the color (16.42);ammonium (17.91%);iron (40.30%);Nitrites (4.48%);Residual chlorine (91.05). Bacteriologically, the analyses showed a high total aerobic mesophilic flora contamination, faecal coliforms, E. coli, faecal enterococci respectively in 89.55%, 82.09%, 50.75% and 70.15% of the analyzed water samples. The ratio of faecal coliforms to faecal enterococci indicated that the origin of faecal contamination was human in 59.7% of the samples and animal in 40.3% of the samples. The adoption of hygiene measures at the water point, during the transport and storage of water, including the treatment by chlorination of drinking water at the family level was recommended for the population concerned and household awareness on the adoption of basic hygiene and sanitation measures have been recommended for hygiene and sanitation services.
文摘To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.
文摘Background: Knowledge of the common bacteria that cause surgical site infection (SSI) and their antibiotic sensitivity is mandatory if treatment of surgical infection is to be successful. The threat of the emergence of resistant strains of bacteria is ever-present. Hence, a sensitivity directed therapy is paramount for the successful eradication of organisms with minimal risk of development of antibiotic resistance. Aim: The aim is to identify the common bacteria that cause SSI in orthopaedic implant surgeries in our hospital. Method: This is a prospective longitudinal study that includes all orthopaedic surgeries involving the use of implants within one year. Patients that had major orthopaedic surgeries involving implant were followed up and their wounds inspected for signs of SSI on postoperative days 3, 7, 14, 42 and 90. Wound swab was taken for microscopy, culture and sensitivity analysis from those who had wound infection, based on the CDC guidelines. Results: One-hundred and sixteen patients met our inclusion criteria and were included in the analysis. There were 62 males and 54 females. The mean age of the participant was 39.62 years (SD = 15.02 years). Fracture fixation with plates and screws was the most common implant surgery done. The incidence of SSI was 2.6%, and Escherichia coli was the most common isolated pathogen. All the SSIs were superficial incisional type, and the infection was monomicrobial in 67% of cases and polymicrobial in 33%. All of the isolated pathogens were sensitive to Imipenem and Gentamycin. Conclusion: Superficial incisional SSI is the most common type of SSI in this study. Escherichia coli is the most frequent pathogen in SSI affecting implant surgeries in our hospital. Gentamycin and Imipenem should be used for the prophylaxis of SSI in our environment.
文摘The drinking water for all the inhabitants of Tangier is still an aim far from being achieved. Therefore, all rural areas and some urban areas feed water wells and springs whose composition and safety are unknown. In the absence of data on water and ignorance of consumers towards the risk of poisoning and waterborne diseases transmitted by these waters, the authors conducted a study to determine the composition, physico-chemical quality and bacteriological these waters. This study is to provide a basic physico-chemical and bacteriological data from these waters in some urban areas of old or missing infrastructure in some sites in the province of Tangier. Such as Ziatine sites, Gzenaya, Rmilate, Malabata ＋ Mnar, Mghoura ＋ Aouama and Downtown. Analyzes are performed according to AFNOR methods （AFNOR： French Standards Association）. The results indicate that these waters are generally mild and are experiencing significant geochemical imbalance. Moreover, these waters also have a significant organic and bacteriological pollution. It is concluded that the waters of these sites are of poor quality, unfit for human consumption.
