Site-specific mutagenesis of bacteriorhodopsin(bR)is an importanttechnique for studying the niechanism of pruton-pumping and optic-electronic fea-turee of bR molecules.The bop gene niutant with une nucleutide substitu...Site-specific mutagenesis of bacteriorhodopsin(bR)is an importanttechnique for studying the niechanism of pruton-pumping and optic-electronic fea-turee of bR molecules.The bop gene niutant with une nucleutide substitutiun of G→A at position 286 is constructed and expressed in Halubacterium HalubiuM L33(bop-).The result of mutation is identified by DNA sequencing, western blottingand absorption spectra analysis.The altered bup gene can be inherited stably in L33cells.展开更多
文摘Site-specific mutagenesis of bacteriorhodopsin(bR)is an importanttechnique for studying the niechanism of pruton-pumping and optic-electronic fea-turee of bR molecules.The bop gene niutant with une nucleutide substitutiun of G→A at position 286 is constructed and expressed in Halubacterium HalubiuM L33(bop-).The result of mutation is identified by DNA sequencing, western blottingand absorption spectra analysis.The altered bup gene can be inherited stably in L33cells.
