期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Identification of a 12.5-kD Protein From Caudate-Putamen Nucleus as a Dopaminergic Neuronotrophic Factor 被引量:1
1
作者 周明华 任峰 赵丽萍 《Science China Chemistry》 SCIE EI CAS 1994年第11期1360-1366,共7页
The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on the dopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densities for the growth of DA neuron culture... The effect of the extract from caudate-putamen nucleus of newborn rats(CPe)on the dopaminergic(DA)neurons has been studied.An MTT colorimetric microassay measuring optical densities for the growth of DA neuron cultures reveals a significant increase in growth for cultures with CPe as com- pared to those without CPe(p<0.05).Rhodamine retrograde-prelabelled DA neurons were cultured on a Phastgel containing all the electrophoretically separated protein bands from CPe,and able to fish out from it their own trophic factor,a 12.5-kD protein band.The survived neurons at the 12.5-kD protein band were immunostained positive with anti-dopamine antibody.Co-culturing the 12.5 kD-containing gel strip with ex- plants from substantia nigra at a close distance for a week revealed anti-dopamine immunopositive neurites outgrowing from the explants only towards the 12.5-kD gel strip.These results indicate that a 12.5-kD pro- tein from the CPe is capable of maintaining the survival of DA neurons of the substantia nigra and promoting their neurite outgrowth. 展开更多
关键词 CAUDATE-PUTAMEN substantia nigra DOPAMINERGIC neuron TROPHIC factor PROTEIN band-fishing by cells
原文传递
EFFECT OF 22kD,35kD PROTEIN MOLECULES FROM EXTRACT OF SKELETAL MUSCLE ON CULTURED ANTERIOR HORN MOTONEURON OF LUMBAR SPINE IN RAT
2
作者 周明华 吴玺印 +4 位作者 任峰 赵丽萍 黄威权 杨执庸 任麟孙 《Chinese Science Bulletin》 SCIE EI CAS 1992年第20期1742-1746,共5页
Ⅰ. INTRODUCTION The survival of motoneurons in vertebrates during their embryonic period depends on certain neurotrophic substances provided from their skeletal muscles. Skeletal muscles have been shown, in in vitro ... Ⅰ. INTRODUCTION The survival of motoneurons in vertebrates during their embryonic period depends on certain neurotrophic substances provided from their skeletal muscles. Skeletal muscles have been shown, in in vitro and in vivo experiments, to contain substances which are capable of enhancing the survival and development of motoneurons but preventing the 展开更多
关键词 protein band-fishing by cells motoneuronotrophic factor ANTERIOR HORN MOTONEURON SKELETAL muscle EXTRACT phast system gel electrophoresis
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部