The aim of the study was to prepare berberine hydrochloride long-circulating liposomes and optimize the formulation and process parameters,and investigate the influence of different factors on the encapsulation effici...The aim of the study was to prepare berberine hydrochloride long-circulating liposomes and optimize the formulation and process parameters,and investigate the influence of different factors on the encapsulation efficiency.Berberine hydrochloride liposomes were prepared in response to a transmembrane ion gradient that was established by ionophore A23187.Free and liposomal drug were separated by cation exchange resin,and then the amount of intraliposomal berberine hydrochloride was determined by UV spectrophotometry.The optimized encapsulation efficiency of berberine hydrochloride liposomes was 94.3%2.1%when the drug-to-lipid ratio was 1:20,and the mean diameter was 146.9 nm3.2 nm.As a result,the ionophore A23187-mediated ZnSO_(4)gradient method was suitable for the preparation of berberine hydrochloride liposomes that we could get the desired encapsulation efficiency and drug loading.展开更多
Objective:To evaluate the clinical efficacy of berberine hydrochloride in the treatment of ulcerative colitis and its influence on inflammatory factors.Methods:We searched VIP Chinese Journal Database(VIP),China Biome...Objective:To evaluate the clinical efficacy of berberine hydrochloride in the treatment of ulcerative colitis and its influence on inflammatory factors.Methods:We searched VIP Chinese Journal Database(VIP),China Biomedical Literature Database(CBM),Wanfang Data,China Knowledge Network(CNKI),and searched English databases including:PubMed,Embase,Web of Science,and Cochrane Library for randomized controlled studies of berberine hydrochloride in the treatment of ulcerative colitis.The retrieval time was from the establishment of the database to April 28,2021.After the quality evaluation of the selected literature,the RevMan5.3 software was used for statistical analysis.Results:Eight studies were included,involving 658 patients with ulcerative colitis.Meta analysis showed that the effective rate of the treatment group was higher than that of the control group,and the difference was statistically significant[OR=6.41,95%CI(3.25,12.65),Z=5.36,P<0.00001];IL-6[MD=-13.64,95%CI(-14.88,-12.41),Z=3.99,P<0.00001],IL-8[MD=-13.41,95%CI(-15.11,-11.71),Z=15.46,P<0.00001],tumor necrosis factor[MD=-5.62,95%CI(-8.35,-2.89),Z=4.03,P<0.0001],and disease activity index[MD=-2.34,95%CI(-2.55,-2.14),Z=22.37,P<0.00001]in the treatment group were lower than the control group,the difference was statistically significant;IL-10[MD=6.49,95%CI(5.64,7.35),Z=14.86,P<0.00001]in the treatment group was higher than the control group,and the difference was statistically significant;abdominal pain relief time[MD=-3.41,95%CI(-4.02,-2.79),Z=10.84,P<0.00001],diarrhea relief time[MD=-2.5,95%CI(-2.86,-2.15),Z=13.87,P<0.00001],pus and blood stool remission time[MD=-2.44,95%CI(-2.87,-2.00).Z=10.87,P<0.00001]in the treatment group were shorter than the control group,and the difference was statistically significant.Conclusion:Berberine hydrochloride can assist in the treatment of ulcerative colitis by reducing the level of inflammatory factors.展开更多
Objective:Hemorrhoidal disease(HD)is the most common proctological disease,with an estimated prevalence rate of 4.4%,and a peak in individuals between 45 and 65 years of age.This study was done to evaluate whether Lia...Objective:Hemorrhoidal disease(HD)is the most common proctological disease,with an estimated prevalence rate of 4.4%,and a peak in individuals between 45 and 65 years of age.This study was done to evaluate whether Lian-Zhi-San(LZS),a clinically used anti-hemorrhoidal ointment could alleviate the inflammatory injury,with its associated changes of inflammatory cytokines and morphology of anorectal tissues,in an experimental model of HD in rats.Methods:HD was induced by croton oil preparation(COP)applied to the anorectal region.Rats were then treated with cotton swabs soaked in LZS ointment,water or white vaseline,twice a day for 7 d.At the end of the experiment,HD was evaluated by measuring hemorrhoidal and biochemical parameters along with histopathological observations.Results:In this study,COP induced a significant increase in the macroscopic severity score,anorectal coefficient and Evans blue extravasation,compared to normal rats.Additionally,it greatly enhanced the expression and secretion levels of some important inflammation-related cytokines along with marked histological damage,compared to normal rats.Rats treated with LZS ointment experienced significantly ameliorated Evans blue extravasation(P<0.05),decreased macroscopic severity score(0.86±0.14 vs.1.65±0.16)and the anorectal coefficient(P<0.01);its use also attenuated tissue damage and inhibited the expression and secretion levels of inflammation-related cytokines(interleukin-1 b,interleukin-6 and tumor necrosis factor-a).Conclusion:This study validates a preliminary understanding of the use of LZS ointment to treat inflammatory factors and tissue damage in an experimental model of HD in rats.展开更多
文摘The aim of the study was to prepare berberine hydrochloride long-circulating liposomes and optimize the formulation and process parameters,and investigate the influence of different factors on the encapsulation efficiency.Berberine hydrochloride liposomes were prepared in response to a transmembrane ion gradient that was established by ionophore A23187.Free and liposomal drug were separated by cation exchange resin,and then the amount of intraliposomal berberine hydrochloride was determined by UV spectrophotometry.The optimized encapsulation efficiency of berberine hydrochloride liposomes was 94.3%2.1%when the drug-to-lipid ratio was 1:20,and the mean diameter was 146.9 nm3.2 nm.As a result,the ionophore A23187-mediated ZnSO_(4)gradient method was suitable for the preparation of berberine hydrochloride liposomes that we could get the desired encapsulation efficiency and drug loading.
基金This study was supported by the National Natural Science Foundation of China(No.81473689)the National Natural Science Foundation of Beijing(No.7202112)。
文摘Objective:To evaluate the clinical efficacy of berberine hydrochloride in the treatment of ulcerative colitis and its influence on inflammatory factors.Methods:We searched VIP Chinese Journal Database(VIP),China Biomedical Literature Database(CBM),Wanfang Data,China Knowledge Network(CNKI),and searched English databases including:PubMed,Embase,Web of Science,and Cochrane Library for randomized controlled studies of berberine hydrochloride in the treatment of ulcerative colitis.The retrieval time was from the establishment of the database to April 28,2021.After the quality evaluation of the selected literature,the RevMan5.3 software was used for statistical analysis.Results:Eight studies were included,involving 658 patients with ulcerative colitis.Meta analysis showed that the effective rate of the treatment group was higher than that of the control group,and the difference was statistically significant[OR=6.41,95%CI(3.25,12.65),Z=5.36,P<0.00001];IL-6[MD=-13.64,95%CI(-14.88,-12.41),Z=3.99,P<0.00001],IL-8[MD=-13.41,95%CI(-15.11,-11.71),Z=15.46,P<0.00001],tumor necrosis factor[MD=-5.62,95%CI(-8.35,-2.89),Z=4.03,P<0.0001],and disease activity index[MD=-2.34,95%CI(-2.55,-2.14),Z=22.37,P<0.00001]in the treatment group were lower than the control group,the difference was statistically significant;IL-10[MD=6.49,95%CI(5.64,7.35),Z=14.86,P<0.00001]in the treatment group was higher than the control group,and the difference was statistically significant;abdominal pain relief time[MD=-3.41,95%CI(-4.02,-2.79),Z=10.84,P<0.00001],diarrhea relief time[MD=-2.5,95%CI(-2.86,-2.15),Z=13.87,P<0.00001],pus and blood stool remission time[MD=-2.44,95%CI(-2.87,-2.00).Z=10.87,P<0.00001]in the treatment group were shorter than the control group,and the difference was statistically significant.Conclusion:Berberine hydrochloride can assist in the treatment of ulcerative colitis by reducing the level of inflammatory factors.
基金supported by grants from 2018 Annual Program for the Expert Workstation in Healthcare of Jinshan District in Shanghai(No.jszjz2018020Y)the Sixth Period of Key Medical Specialty of Health Commission in Jinshan District in Shanghai(No.JSZK2019A02)。
文摘Objective:Hemorrhoidal disease(HD)is the most common proctological disease,with an estimated prevalence rate of 4.4%,and a peak in individuals between 45 and 65 years of age.This study was done to evaluate whether Lian-Zhi-San(LZS),a clinically used anti-hemorrhoidal ointment could alleviate the inflammatory injury,with its associated changes of inflammatory cytokines and morphology of anorectal tissues,in an experimental model of HD in rats.Methods:HD was induced by croton oil preparation(COP)applied to the anorectal region.Rats were then treated with cotton swabs soaked in LZS ointment,water or white vaseline,twice a day for 7 d.At the end of the experiment,HD was evaluated by measuring hemorrhoidal and biochemical parameters along with histopathological observations.Results:In this study,COP induced a significant increase in the macroscopic severity score,anorectal coefficient and Evans blue extravasation,compared to normal rats.Additionally,it greatly enhanced the expression and secretion levels of some important inflammation-related cytokines along with marked histological damage,compared to normal rats.Rats treated with LZS ointment experienced significantly ameliorated Evans blue extravasation(P<0.05),decreased macroscopic severity score(0.86±0.14 vs.1.65±0.16)and the anorectal coefficient(P<0.01);its use also attenuated tissue damage and inhibited the expression and secretion levels of inflammation-related cytokines(interleukin-1 b,interleukin-6 and tumor necrosis factor-a).Conclusion:This study validates a preliminary understanding of the use of LZS ointment to treat inflammatory factors and tissue damage in an experimental model of HD in rats.
