[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yel...[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(242.22 ±0.71 kg) were randomly divided into four treatments.Cattles were fed with diets of different concentrate to forage ratios(20:80, group Ⅰ;30:70, Group Ⅱ;40:60, group Ⅲ;50:50, group Ⅳ).The trial lasted 62 d.[Result]The daily gain in experimental group Ⅳ was significantly higher than that in experimental group Ⅱ(P<0.05), but the feed gain ratio in experimental group Ⅳ was lower than those in other ex-perimental groups(P>0.05).The feed intake in experimental group Ⅳ was also markedly higher than those in experimental groups Ⅰ and Ⅱ(P<0.05).Compared with the experimental group Ⅱ, the gross profit of experimental group Ⅳ was significantly promoted(P<0.05).The alanine aminotransferase and aspartate aminotransferase activities in experimental group Ⅱ were markedly higher than those in experimental groups Ⅰ, Ⅲ and Ⅳ.[Conclusion]When the dietary concentrate to forage ratios was 50∶50, the production performance of post-fattening Hainan Yellow Cattle was better.展开更多
Background:Salmonella enterica serovar Enteritidis(SE),as a major cause of foodbom illness,infects humans mainly through the egg.However,the symptom of laying hens usually is not typical and hard to diagnosis.In the p...Background:Salmonella enterica serovar Enteritidis(SE),as a major cause of foodbom illness,infects humans mainly through the egg.However,the symptom of laying hens usually is not typical and hard to diagnosis.In the present study,it is studied that the influences of SE infection on layers’ performance,egg quality and blood biochemical indicators.It will help us to improve the strategy to control SE infection in commercial layers.One hundred layers at 20 wk of age were divided into 2 groups,60 hens for experiment and others for control.The experiment group was fed with the dosage of 10~8 CFU SE per hen.The specific PCR was used to detect the deposition of SE.On the 8 d after SE infection,10 hens from the control group and 30 hens from the experimenta group were slaughtered to detect the SE colonization.The production performance,egg quality and blood biochemical indices were also analyzed.Results:The results showed that the colonization rate of SE was highest in caecum contents(55.17%) and lowest in vagina(17.24%).For the eggs the detection rate of SE was highest on the eggshell(80.00%) and lowest in yolk(18.81%).SE infection had no significant influence on production performance and egg qualities(P > 0.05).The difference of laying rate between the experimental and control groups was less than 0.30%,and both were approximately equal to 82.00%.The blood analysis showed that the aspartic aminotransferase(AST) and alanine aminotransferase(ALT) of experimental group was significantly higher than those of control group(P < 0.05).For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively,and ALT values were32.19 U/l and 24.55 U/l.All of coefficients were less than 20%.The colonization of SE in organs increases the enzyme activities of AST and ALT in blood.Conclusions:SE in feed could invade the oviduct and infect the forming eggs.It significantly increased the concentration of ALT and AST in blood.However,SE infection was hard to be observed from the appearances of layer and egg.It might be a dangerous risk to human health.展开更多
[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their ef...[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall( chitin and phenoloxidase),the physiological and biochemical indices acting on the nervous system( Acetylcholinesterase),and the physiological and biochemical indices acting on the digestive system( protease,amylase and lipase) of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.展开更多
A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complet...A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.展开更多
Objective: To explore the changes and clinical significance of biochemical indexes, antioxidant enzymes and related factors in patients with Parkinson. Methods: 50 patients with Parkinson's disease who were treate...Objective: To explore the changes and clinical significance of biochemical indexes, antioxidant enzymes and related factors in patients with Parkinson. Methods: 50 patients with Parkinson's disease who were treated in our hospital from March 2016 to March 2017 were selected as observation group. 50 healthy volunteers who were received physical examination in this hospital during the same period were selected as control group. The levels of bilirubin, blood uric acid, superoxide dismutase, glutathione peroxidase, catalase, TNF-α, tumor necrosis factor-α receptor-1, tumor necrosis factor-α receptor-2, monocyte chemoattractant protein-1 and macrophage inflammatory protein -1 of the patients in the two group were compared. Results: The level of serum bilirubin, uric acid, SOD, GSH-PX and CAT of patients in the observation group were (5.65±0.61) μmoL/L, (262.96±26.38) μmoL/L, (92.65±9.35) U/mL, (18.96±2.03) U/mL and (56.94±16.23) U/mL. The above indexes of patients in the observation group were significantly lower than those of patients in the control group (P<0.05). The serum level of TNF-α, sTNFR1, MCP-1 and MIP-1 of patients in the observation group were (19.65±2.07) pg/mL, (798.96±80.20) pg/mL, (43.65±4.27) ng/L and (23.96±2.41) ng/L. The above indexes of patients in the observation group were significantly higher than those of patients in the control group (P<0.05). Conclusion: Bilirubin, uric acid, antioxidant enzymes and inflammatory factors are all related to the progression of Parkinson's disease and can be used as auxiliary index in the diagnosis and treatment of Parkinson's disease.展开更多
Objective:To explore the significance of cerebrospinal fluid and plasma biochemical indicator ratio in identifying the tuberculous meningitis and purulent meningitis.Methods: A total of 43 patients with tuberculous me...Objective:To explore the significance of cerebrospinal fluid and plasma biochemical indicator ratio in identifying the tuberculous meningitis and purulent meningitis.Methods: A total of 43 patients with tuberculous meningitis who were admitted in our hospital from August 2015 to July 2017 were included in the study and served as the tuberculous meningitis group. Moreover, 40 cases with purulent meningitis were served as the purulent meningitis group. The morning fasting peripheral venous blood was collected and centrifuged for the plasma. Lumbar puncture was used to collect cerebrospinal fluid. The fully automatic biochemical analyzer was used to detect GSH, the plasma protein, chloride, glucose, lactic acid, and ADA. GSF/plasma ratio was calculated. The immunity transmission turbidity was used to detect GSH and Cys-C. FCM was used to detect CD64. ELISA was used to detect MMP-9.Results:GSF chloride and lactic acid in the tuberculous meningitis group were significantly lower than those in the purulent meningitis group, while ADA was significantly higher than that in the purulent meningitis group. The plasma protein, chloride, and lactic acid in the tuberculous meningitis group were significantly lower than those in the purulent meningitis group, while ADA was significantly higher than that in the purulent meningitis group. ADA ratio in GSF in the tuberculous meningitis group was significantly higher than that in the purulent meningitis group, while chloride and lactic acid ratio was significantly lower than that in the purulent meningitis group. CD64 in GSF in the tuberculous meningitis group was significantly lower than that in the purulent meningitis group, and MMP-9 was significantly higher than that in the purulent meningitis group, while the comparison of Cys-C between the two groups was not statistically significant.Conclusions: GSF, plasma protein, chloride, lactic acid, and ADA concentration ratio in the tuberculous meningitis group and purulent meningitis group have a certain difference. Combined detection of CD64 and MMP-9 contribute to identifying the two meningitis.展开更多
[Objective] To observe biological characteristics of brown-egg dwarf layers. [Method] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively....[Objective] To observe biological characteristics of brown-egg dwarf layers. [Method] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively. Fifty-four chickens were detected, nine roosters and nine hens at each age. [Result] As analyzed by t test and multiple comparisons, during 20-60 weeks old, the RBC, HGB, MPV, PDW, LYMPH%, TP and CHO showed an increasing trend. However, during this period, the HCT, MCV, RDW, WBC and LYMPH first decreased and then declined, while the MCH, MCHC, PLT, PCT, MID, MID%, GRAN, GRAN%, AST, ALB, TG, ALT and GLU changed contrarily. The chickens at different ages had significantly different physiological and biochemical indicators (P < 0.05). The RBC, HCT, HGB, WBC, LYMPH and AST were significantly different between the roosters and hens during 20-60 weeks old. [Conclusion] In the brown-egg dwarf layers, the physiological and biochemical indicators vary with aging, and their difference levels also change with aging.展开更多
为提高山区隧道施工场地布置方案决策的准确性,以牛栾村隧道六种施工场地布置方案为例,提出了基于改进灰靶的方案优选模型。首先,通过分析场地布置方案影响因素,构建了以方案可行性、方案经济性、环境影响和社会效益影响为核心的评价指...为提高山区隧道施工场地布置方案决策的准确性,以牛栾村隧道六种施工场地布置方案为例,提出了基于改进灰靶的方案优选模型。首先,通过分析场地布置方案影响因素,构建了以方案可行性、方案经济性、环境影响和社会效益影响为核心的评价指标体系;其次,采用云模型将定性指标定量描述,并运用CRITIC(criteria importance though intercriteria correlation)确定指标权重;最后以灰色关联差异信息值为基础,结合欧几里得理论计算修正的加权靶心距,通过对比靶心距实现方案优选,并采用单因素轮换法(one-at-a-time,OAT)进行了指标敏感性分析。结果表明:最优方案的加权靶心距为0.610,评选出的方案与实际一致,并分析出“地形地貌改变”为对方案评选影响最大的指标。可见,该方法呈现了各方案的优劣,使山区隧道施工场地布置方案评选更科学、合理。展开更多
基金Technical Development Project of Hainan Provincial Scientific Research Institutes(KYYS-2018-05)Special Funds for Central Government Guiding the Development of Local Science and Technology(ZY2019HN01).
文摘[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(242.22 ±0.71 kg) were randomly divided into four treatments.Cattles were fed with diets of different concentrate to forage ratios(20:80, group Ⅰ;30:70, Group Ⅱ;40:60, group Ⅲ;50:50, group Ⅳ).The trial lasted 62 d.[Result]The daily gain in experimental group Ⅳ was significantly higher than that in experimental group Ⅱ(P<0.05), but the feed gain ratio in experimental group Ⅳ was lower than those in other ex-perimental groups(P>0.05).The feed intake in experimental group Ⅳ was also markedly higher than those in experimental groups Ⅰ and Ⅱ(P<0.05).Compared with the experimental group Ⅱ, the gross profit of experimental group Ⅳ was significantly promoted(P<0.05).The alanine aminotransferase and aspartate aminotransferase activities in experimental group Ⅱ were markedly higher than those in experimental groups Ⅰ, Ⅲ and Ⅳ.[Conclusion]When the dietary concentrate to forage ratios was 50∶50, the production performance of post-fattening Hainan Yellow Cattle was better.
基金supported by National System for Layer Production Technology of China(CARS-41)Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China "Study on Poultry Eggs and Meat Detection Technology"(IRT0945)Chinese Universities Scientific Fund(2013QJ069)
文摘Background:Salmonella enterica serovar Enteritidis(SE),as a major cause of foodbom illness,infects humans mainly through the egg.However,the symptom of laying hens usually is not typical and hard to diagnosis.In the present study,it is studied that the influences of SE infection on layers’ performance,egg quality and blood biochemical indicators.It will help us to improve the strategy to control SE infection in commercial layers.One hundred layers at 20 wk of age were divided into 2 groups,60 hens for experiment and others for control.The experiment group was fed with the dosage of 10~8 CFU SE per hen.The specific PCR was used to detect the deposition of SE.On the 8 d after SE infection,10 hens from the control group and 30 hens from the experimenta group were slaughtered to detect the SE colonization.The production performance,egg quality and blood biochemical indices were also analyzed.Results:The results showed that the colonization rate of SE was highest in caecum contents(55.17%) and lowest in vagina(17.24%).For the eggs the detection rate of SE was highest on the eggshell(80.00%) and lowest in yolk(18.81%).SE infection had no significant influence on production performance and egg qualities(P > 0.05).The difference of laying rate between the experimental and control groups was less than 0.30%,and both were approximately equal to 82.00%.The blood analysis showed that the aspartic aminotransferase(AST) and alanine aminotransferase(ALT) of experimental group was significantly higher than those of control group(P < 0.05).For experimental and control groups AST values were 236.22 U/l and 211.84 U/l respectively,and ALT values were32.19 U/l and 24.55 U/l.All of coefficients were less than 20%.The colonization of SE in organs increases the enzyme activities of AST and ALT in blood.Conclusions:SE in feed could invade the oviduct and infect the forming eggs.It significantly increased the concentration of ALT and AST in blood.However,SE infection was hard to be observed from the appearances of layer and egg.It might be a dangerous risk to human health.
基金Supported by Open Fund of Key Laboratory of Contaminated Environment Con-trol and Regional Ecology Safety(SYU-KF-L-05)Science and TechnologyProject of Liaoning Province(2012214001)
文摘[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall( chitin and phenoloxidase),the physiological and biochemical indices acting on the nervous system( Acetylcholinesterase),and the physiological and biochemical indices acting on the digestive system( protease,amylase and lipase) of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.
基金supported financially by the project‘Research and Development on Technology and Key Equipment for New Type of Industrialized and Healthy Animal Husbandry'funded by the Chinese Ministry of Science and Technology(2006BAD 14B02-6)11th Five Years Key Programs for Science and Technology Development of China(2006BAD01A08-07)+1 种基金Hubei Province Key Project of Science and Technology (2006AA201B24)Wuhan City Key Project of Industrialization(200720112026)
文摘A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.
文摘Objective: To explore the changes and clinical significance of biochemical indexes, antioxidant enzymes and related factors in patients with Parkinson. Methods: 50 patients with Parkinson's disease who were treated in our hospital from March 2016 to March 2017 were selected as observation group. 50 healthy volunteers who were received physical examination in this hospital during the same period were selected as control group. The levels of bilirubin, blood uric acid, superoxide dismutase, glutathione peroxidase, catalase, TNF-α, tumor necrosis factor-α receptor-1, tumor necrosis factor-α receptor-2, monocyte chemoattractant protein-1 and macrophage inflammatory protein -1 of the patients in the two group were compared. Results: The level of serum bilirubin, uric acid, SOD, GSH-PX and CAT of patients in the observation group were (5.65±0.61) μmoL/L, (262.96±26.38) μmoL/L, (92.65±9.35) U/mL, (18.96±2.03) U/mL and (56.94±16.23) U/mL. The above indexes of patients in the observation group were significantly lower than those of patients in the control group (P<0.05). The serum level of TNF-α, sTNFR1, MCP-1 and MIP-1 of patients in the observation group were (19.65±2.07) pg/mL, (798.96±80.20) pg/mL, (43.65±4.27) ng/L and (23.96±2.41) ng/L. The above indexes of patients in the observation group were significantly higher than those of patients in the control group (P<0.05). Conclusion: Bilirubin, uric acid, antioxidant enzymes and inflammatory factors are all related to the progression of Parkinson's disease and can be used as auxiliary index in the diagnosis and treatment of Parkinson's disease.
文摘Objective:To explore the significance of cerebrospinal fluid and plasma biochemical indicator ratio in identifying the tuberculous meningitis and purulent meningitis.Methods: A total of 43 patients with tuberculous meningitis who were admitted in our hospital from August 2015 to July 2017 were included in the study and served as the tuberculous meningitis group. Moreover, 40 cases with purulent meningitis were served as the purulent meningitis group. The morning fasting peripheral venous blood was collected and centrifuged for the plasma. Lumbar puncture was used to collect cerebrospinal fluid. The fully automatic biochemical analyzer was used to detect GSH, the plasma protein, chloride, glucose, lactic acid, and ADA. GSF/plasma ratio was calculated. The immunity transmission turbidity was used to detect GSH and Cys-C. FCM was used to detect CD64. ELISA was used to detect MMP-9.Results:GSF chloride and lactic acid in the tuberculous meningitis group were significantly lower than those in the purulent meningitis group, while ADA was significantly higher than that in the purulent meningitis group. The plasma protein, chloride, and lactic acid in the tuberculous meningitis group were significantly lower than those in the purulent meningitis group, while ADA was significantly higher than that in the purulent meningitis group. ADA ratio in GSF in the tuberculous meningitis group was significantly higher than that in the purulent meningitis group, while chloride and lactic acid ratio was significantly lower than that in the purulent meningitis group. CD64 in GSF in the tuberculous meningitis group was significantly lower than that in the purulent meningitis group, and MMP-9 was significantly higher than that in the purulent meningitis group, while the comparison of Cys-C between the two groups was not statistically significant.Conclusions: GSF, plasma protein, chloride, lactic acid, and ADA concentration ratio in the tuberculous meningitis group and purulent meningitis group have a certain difference. Combined detection of CD64 and MMP-9 contribute to identifying the two meningitis.
基金supported by the grants from the Earmarked Fund for Modern Agro-industry (Layer Industry) Technology Research System (nycytx-41-g19)the National Key Technology R&D Program (2008BAD2B06)
文摘[Objective] To observe biological characteristics of brown-egg dwarf layers. [Method] Physiological and biochemical indicators of brown-egg dwarf layers were determined at the age of 20, 40 and 60 weeks, respectively. Fifty-four chickens were detected, nine roosters and nine hens at each age. [Result] As analyzed by t test and multiple comparisons, during 20-60 weeks old, the RBC, HGB, MPV, PDW, LYMPH%, TP and CHO showed an increasing trend. However, during this period, the HCT, MCV, RDW, WBC and LYMPH first decreased and then declined, while the MCH, MCHC, PLT, PCT, MID, MID%, GRAN, GRAN%, AST, ALB, TG, ALT and GLU changed contrarily. The chickens at different ages had significantly different physiological and biochemical indicators (P < 0.05). The RBC, HCT, HGB, WBC, LYMPH and AST were significantly different between the roosters and hens during 20-60 weeks old. [Conclusion] In the brown-egg dwarf layers, the physiological and biochemical indicators vary with aging, and their difference levels also change with aging.
文摘为提高山区隧道施工场地布置方案决策的准确性,以牛栾村隧道六种施工场地布置方案为例,提出了基于改进灰靶的方案优选模型。首先,通过分析场地布置方案影响因素,构建了以方案可行性、方案经济性、环境影响和社会效益影响为核心的评价指标体系;其次,采用云模型将定性指标定量描述,并运用CRITIC(criteria importance though intercriteria correlation)确定指标权重;最后以灰色关联差异信息值为基础,结合欧几里得理论计算修正的加权靶心距,通过对比靶心距实现方案优选,并采用单因素轮换法(one-at-a-time,OAT)进行了指标敏感性分析。结果表明:最优方案的加权靶心距为0.610,评选出的方案与实际一致,并分析出“地形地貌改变”为对方案评选影响最大的指标。可见,该方法呈现了各方案的优劣,使山区隧道施工场地布置方案评选更科学、合理。
