In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosenso...In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosensor.ZnO nanoparticles have been prepared via the green route using olive leaves extract as a reductant.ZnO/Ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole(Py)monomer using ferric chloride(FeCl3)as an oxidizing agent.The produced materials and the composite films were characterized using X-ray diffraction analysis(XRD),scanning electron microscope(SEM),Fourier transform infrared(FTIR)and thermogravimetric analysis(TGA).Glucose oxidase was successfully immobilized on the surface of the prepared film and then ZnO/Ppy/CA/GOx composite was sputtered with platinum electrode for the current determination at different initial concentrations of glucose.Current measurements proved the suitability and the high sensitivity of the constructed biosensor for the detection of glucose levels in different samples.The performance of the prepared biosensor has been assessed by measuring and comparing glucose concentrations up to 800 ppm.The results affirmed the reliability of the developed biosensor towards real samples which suggests the wide-scale application of the proposed biosensor.展开更多
Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and...Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and protecting public health.However,handling complex samples such as human and wastewater can be challenging due to their dynamic and complex composition and the ultralow concentration of target analytes.This review presents a comprehensive overview of the latest breakthroughs in waterborne virus biosensors.It begins by highlighting several promising strategies that enhance the sensing performance of optical and electrochemical biosensors in human samples.These strategies include optimizing bioreceptor selection,transduction elements,signal amplification,and integrated sensing systems.Furthermore,the insights gained from biosensing waterborne viruses in human samples are applied to improve biosensing in wastewater,with a particular focus on sampling and sample pretreatment due to the dispersion characteristics of waterborne viruses in wastewater.This review suggests that implementing a comprehensive system that integrates the entire waterborne virus detection process with high-accuracy analysis could enhance virus monitoring.These findings provide valuable insights for improving the effectiveness of waterborne virus detection,which could have significant implications for public health and environmental management.展开更多
Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures...Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures.Rapid,sensitive,efficient and portable detection method is highly essential for fish viral diseases detection.Biosensor strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and selectivity.With the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids fisheries.This paper reviews the current detection development of salmonids viral diseases.The present limitations and challenges of salmonids virus diseases surveillance and early detection are presented.Novel nucleic acid semiconductor biosensors are briefly reviewed.The perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.展开更多
The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the pr...The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.展开更多
With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monit...With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monitoring,and pre-diagnostics.This paper reviews the recent progress in sweat biosensors and sensing systems integrated into textiles for wearable body status monitoring.The mechanisms of biosensors that are commonly adopted for biomarkers analysis are first introduced.The classification,fabrication methods,and applications of textile conductors in different configurations and dimensions are then summarized.Afterward,innovative strategies to achieve efficient sweat collection with textile-based sensing patches are presented,followed by an in-depth discussion on nanoengineering and system integration approaches for the enhancement of sensing performance.Finally,the challenges of textile-based sweat sensing devices associated with the device reusability,washability,stability,and fabrication reproducibility are discussed from the perspective of their practical applications in wearable healthcare.展开更多
Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in t...Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.展开更多
Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and ...Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.展开更多
We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relat...We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relationship between biosensors. A parallel study of biosensors (edible cucumber slices) had also been conducted, and we found that the circadian rhythm of gas concentrations emitted from biosensors changes seasonally. The pyramid power and Bio-Entanglement did not change the number of cycles in the periodic approximation curve representing circadian rhythm. Therefore, in this paper we analyzed the influence of the pyramid power and Bio-Entanglement, i.e., their influence on the phase, amplitude, and correlation coefficient of the periodic approximation curve representing the circadian rhythm of emitted gas concentrations. The main results are as follows. 1) The pyramid power shifted the phase of the periodic approximation curve representing the circadian rhythm by 43 minutes. 2) The amplitude of the periodic approximation curve changed with the pyramid power and the Bio-Entanglement. The effect on the lower and upper sections of the biosensors stacked in two layers was different, with a tendency to increase the amplitude of the lower layer and decrease the amplitude of the upper layer. 3) The pyramid power and the Bio-Entanglement affected the correlation coefficient between gas concentration and the periodic approximation curve representing the circadian rhythm of gas concentration. The effect on the lower and upper layers of the biosensors was different, with a tendency for the lower layer correlation coefficient to be larger and the upper layer correlation coefficient to be smaller. Previously we demonstrated that the pyramid power and the Bio-Entanglement affect the ratio of gas concentration, i.e., psi index Ψ. In this paper we demonstrate for the first time that the pyramid power and the Bio-Entanglement affect time, i.e., phase difference.展开更多
Exposure to toxins can lead to a wide range of adverse health effects, including respiratory problems, neurological disorders, cancer, and reproductive issues. Toxins can come from various sources, such as industrial ...Exposure to toxins can lead to a wide range of adverse health effects, including respiratory problems, neurological disorders, cancer, and reproductive issues. Toxins can come from various sources, such as industrial waste, agricultural runoff, and household chemicals. Therefore, detecting and monitoring toxins in the environment is crucial for protecting human health and the environment. This study aimed to evaluate the performance of Hememics biosensor system in detecting environmental toxins such as Ricin and Staphylococcal enterotoxin B (SEB) in mixed matrixes. When Ricin and SEB are spiked into soil, chopped lettuce, tap water, milk and serum, the biosensor was able to detect these toxins, without sample processing, at a level of detection comparable to lab testing with high sensitivity and specificity. Furthermore, Hememics biosensor system is designed to be network-enabled, which means that results can be transmitted to relevant agencies for quick decisions. This feature is crucial in cases where quick action is needed to prevent further contamination or exposure to harmful toxins.展开更多
Fast and precise diagnostic techniques are required for the treatment of many disorders.Biosensors are one of the diagnostic devices that are applicable in biological and medical sciences.Biosensors could be utilized ...Fast and precise diagnostic techniques are required for the treatment of many disorders.Biosensors are one of the diagnostic devices that are applicable in biological and medical sciences.Biosensors could be utilized to recognize biological molecules with high sensitivity.Biosensors are consisted of different components and have different types.Each type of biosensor is used in a particular field according to its specific features.Nanobodies are a novel class of antibodies with small size,high affinity,and specificity to their target.The unique properties of nanobodies make them appropriate tools for diagnostic applications.In this paper,we review biosensors,and their features and roles in medicine.Antibody/nanobody-based biosensors are also specifically discussed.展开更多
Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applica...Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applications of advanced nanomaterials in biosensors.Every year there are several advances in sensing techniques that can be attributed to nanomaterials,biorecognition elements,or their related fabrication techniques.The further development of nanotechnology-based sensors provides a wide variety of opportunities to modern research.Advanced nanomaterials can provide remarkable optical,electrical,mechanical,and catalytic properties.For example,transition metals and organic polymers have been used in the fabrication of powerful,sensitive,and precise biosensors.The distinctive properties of advanced nanomaterials have been widely incorporated into biosensors.However,fabrication techniques also play important roles in the development of these devices.Therefore,we present a review of some of the advanced nanomaterials that have been widely used over the last few years and discuss their fabrication techniques.The focus of this review is to provide a directional perspective of recently fabricated advanced nanomaterial-based biosensors in the diagnosis of various diseases.展开更多
A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical resea...A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical research,for example,the blood glucose biosensor.This review focuses on the current state of biosensor research with respect to efficient,specific and rapid detection of hepatitis B virus(HBV).The biosensors developed based on different techniques,including optical methods(e.g.,surface plasmon resonance),acoustic wave technologies(e.g.,quartz crystal microbalance),electrochemistry(amperometry,voltammetry and impedance) and novel nanotechnology,are also discussed.展开更多
An amperometric hydrogen peroxide biosensor using a nanobiocomposite based on neutral red modified carbon nanotubes and co-immobilized glucose oxidase and horseradish peroxidase is reported. Modification of the nanobi...An amperometric hydrogen peroxide biosensor using a nanobiocomposite based on neutral red modified carbon nanotubes and co-immobilized glucose oxidase and horseradish peroxidase is reported. Modification of the nanobiocomposite electrode with neutral red resulted in a sensitive, low-cost and reliable H_2O_2 sensor. The use of carbon nanotubes, as the conductive part of the composite, facilitated fast electron transfer rates. The biosensor was characterized for the influence of p H, potential and temperature. A remarkable feature of the biosensor is the detection of H_2O_2 at low applied potentials where the noise level and interferences are minimal. The sensor has a fast steady-state measuring time of 10 s with a quick response(2 s). The biosensor showed a linear range from 15 n M to 45 m M of H_2O_2 and a detection limit of 5 n M. Nafion, which is used as a binder, makes the determination free from other electroactive substances. The repeatability, reproducibility,stability and analytical performance of the sensor are very good.展开更多
Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites ...Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites pose a potential risk of allergic reactions,bacterial resistance,and increased cancer incidence.Residual antibiotics and the resulting bacterial antibiotic resistance have been recognized as a global challenge that has attracted increasing attention.Therefore,monitoring antibiotics is a critical way to limit the ecological risks from antibiotic pollution.Accordingly,it is desirable to devise new analytical platforms to achieve efficient antibiotic detection with excellent sensitivity and specificity.Quantum dots(QDs)are regarded as an ideal material for use in the development of antibiotic detection biosensors.In this review,we characterize different types of QDs,such as silicon,chalcogenide,carbon,and other doped QDs,and summarize the trends in QD-based antibiotic detection.QD-based sensing applications are classified according to their recognition strategies,including molecularly imprinted polymers(MIPs),aptamers,and immunosensors.We discuss the advantages of QD-derived antibiotic sensors,including low cost,good sensitivity,excellent stability,and fast response,and illustrate the current challenges in this field.展开更多
Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this...Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery,biopharmaceutical manufacturing,gene therapy and tissue engineering,etc.In this review,we focus on current technologies of synthetic biosensors for disease detection.We start with the design principle of synthetic biosensors.Then we move towards the characteristics of simple synthetic biosensors,which can respond to a single input signal,and complex synthetic biosensors including Boolean gate biosensors,cascade biosensors,time-delay biosensors,oscillator biosensors and hysteretic biosensors,which can respond to more than two input signals and perform complex tasks.Synthetic biosensor has showed great potential in disease detection,but it is still in its infancy stage.More efforts should be made in identifying and constructing clinically relevant regulation systems.Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor.The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene-and cell-based therapies.展开更多
A novel quartz crystal microbalance (QCM) biosensor for the determination of organophosphorus pesticide,such as dichlorvos (DDVP),has been fabricated with poly (3,4-ethylenedioxythiophene)/Poly (4-styrenesulfonate) (P...A novel quartz crystal microbalance (QCM) biosensor for the determination of organophosphorus pesticide,such as dichlorvos (DDVP),has been fabricated with poly (3,4-ethylenedioxythiophene)/Poly (4-styrenesulfonate) (PEDOT/PSS) and cholinesterase butyl (BuChE).It is found that PEDOT/PSS coated sensor exhibits the highest sensitivity for DDVP among PEDOT,polyvinyl pyrrolidone,polyvinyl alcohol and polyacrylic acid sensor series.The conditions to prepare PEDOT/PSS coated sensor are optimized.The sensitivity of the PEDOT/PSS hiosensor with BuChE is higher than the others without BuChE The determination of dichlorvos in the range from 400μg/g to 2400μg/g used PEDOT/PSS with BuChE coated hiosensor was obtained.展开更多
Nickel ferrite(NiFe_2O_4) nanoparticles have been dispersed in chitosan solution in order to fab ricate nanocomposite films.Horseradish peroxidase(HRP) has been immobilized onto this chitosan NiFe_2O_4 nanocomposite f...Nickel ferrite(NiFe_2O_4) nanoparticles have been dispersed in chitosan solution in order to fab ricate nanocomposite films.Horseradish peroxidase(HRP) has been immobilized onto this chitosan NiFe_2O_4 nanocomposite film via physical adsorption.The size of the NiFe_2O_4 nanoparticles has been estimated us ing X ray diffraction pattern and scanning electron microscopy(SEM) to be 40±9 nm.The chitosan NiFe_2O_4 nanocomposite film and HRP/chitosan NiFe_2O_4 bioelectrode have been characterized using SEM technique.The HRP/chitosan NiFe_2O_4 nanocomposite bioelectrode has a response time of 4 s,linearity as 0.3 to 12 m M of H2O2,sensitivity as 22 n A/m M.The effects of p H and the temperature of the immobilized HRP electrode have also been studied.展开更多
In this paper, an amperometric acetylcholinesterase(ACh E) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly(diallyldimethy-lammonium chloride)-multi-walled carbon nanotubes-graphen...In this paper, an amperometric acetylcholinesterase(ACh E) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly(diallyldimethy-lammonium chloride)-multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode(GCE) surface, then ACh E was immobilized onto the modified GCE to fabricate the ACh E biosensor. The morphologies and electrochemistry properties of the prepared ACh E biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the ACh E activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.展开更多
Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent ...Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent feed contamination.Traditional detection methods can no longer meet the needs of massive,real-time,simple,and fast mycotoxin monitoring.Rapid detection methods based on advanced material and sensor technology are the future trend.In this review,we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods,especially for simultaneous multiplex mycotoxin determination.Immunoassays,biosensors,and the prominent roles of nanomaterials are introduced.The principles of different types of recognition and signal transduction are explained,and the merits and pitfalls of these methods are compared.Furthermore,limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.展开更多
文摘In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosensor.ZnO nanoparticles have been prepared via the green route using olive leaves extract as a reductant.ZnO/Ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole(Py)monomer using ferric chloride(FeCl3)as an oxidizing agent.The produced materials and the composite films were characterized using X-ray diffraction analysis(XRD),scanning electron microscope(SEM),Fourier transform infrared(FTIR)and thermogravimetric analysis(TGA).Glucose oxidase was successfully immobilized on the surface of the prepared film and then ZnO/Ppy/CA/GOx composite was sputtered with platinum electrode for the current determination at different initial concentrations of glucose.Current measurements proved the suitability and the high sensitivity of the constructed biosensor for the detection of glucose levels in different samples.The performance of the prepared biosensor has been assessed by measuring and comparing glucose concentrations up to 800 ppm.The results affirmed the reliability of the developed biosensor towards real samples which suggests the wide-scale application of the proposed biosensor.
基金supported by the Research Center for Industries of the Future of Westlake University,China(Grant No.:210230006022219/001)the National Natural Science Foundation of China(Grant No.:82104122)+1 种基金Westlake University,China(Grant No.:10318A992001)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang,China(Grant No.:2020R01005).
文摘Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and protecting public health.However,handling complex samples such as human and wastewater can be challenging due to their dynamic and complex composition and the ultralow concentration of target analytes.This review presents a comprehensive overview of the latest breakthroughs in waterborne virus biosensors.It begins by highlighting several promising strategies that enhance the sensing performance of optical and electrochemical biosensors in human samples.These strategies include optimizing bioreceptor selection,transduction elements,signal amplification,and integrated sensing systems.Furthermore,the insights gained from biosensing waterborne viruses in human samples are applied to improve biosensing in wastewater,with a particular focus on sampling and sample pretreatment due to the dispersion characteristics of waterborne viruses in wastewater.This review suggests that implementing a comprehensive system that integrates the entire waterborne virus detection process with high-accuracy analysis could enhance virus monitoring.These findings provide valuable insights for improving the effectiveness of waterborne virus detection,which could have significant implications for public health and environmental management.
基金supported by the National Key Research and Development Program of China(2022YFC2601304)National Key Research and Development Program of China(2022YFC2602100)。
文摘Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures.Rapid,sensitive,efficient and portable detection method is highly essential for fish viral diseases detection.Biosensor strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and selectivity.With the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids fisheries.This paper reviews the current detection development of salmonids viral diseases.The present limitations and challenges of salmonids virus diseases surveillance and early detection are presented.Novel nucleic acid semiconductor biosensors are briefly reviewed.The perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.
基金supported by the National Key Research and Development Program of China(2022YFC2602100)supported by National key research and development program(2021YFC2600602)。
文摘The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.
基金supported by the National Natural Science Foundation of China(62201243)Fundamental and Applied Research Grant of Guangdong Province(2021A1515110627)+3 种基金Southern University of Science and Technology(Y01796108,Y01796208)RGC Senior Research Fellow Scheme of Hong Kong(SRFS2122-5S04)the Hong Kong Polytechnic University(1-ZVQM),RI-Wear of PolyU(1-CD44)Shenzhen Science and Technology Innovation Committee(SGDX20210823103403033).
文摘With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monitoring,and pre-diagnostics.This paper reviews the recent progress in sweat biosensors and sensing systems integrated into textiles for wearable body status monitoring.The mechanisms of biosensors that are commonly adopted for biomarkers analysis are first introduced.The classification,fabrication methods,and applications of textile conductors in different configurations and dimensions are then summarized.Afterward,innovative strategies to achieve efficient sweat collection with textile-based sensing patches are presented,followed by an in-depth discussion on nanoengineering and system integration approaches for the enhancement of sensing performance.Finally,the challenges of textile-based sweat sensing devices associated with the device reusability,washability,stability,and fabrication reproducibility are discussed from the perspective of their practical applications in wearable healthcare.
基金funded by Universiti Putra Malaysia through the Geran Inisiatif Putra Siswazah (GP-IPS/2019/9678200)。
文摘Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.
基金the National Natural Science Foundation of China(Grant No.21705087)Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province(096-1622002)+2 种基金Research Foundation for Distinguished Scholars of Qingdao Agricultural University(663-1117015)the Postgraduate Innovation Program of Qingdao Agricultural University(QNYCX21069)the National Innovation Training Program for College Students(No.202210435030).
文摘Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.
文摘We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relationship between biosensors. A parallel study of biosensors (edible cucumber slices) had also been conducted, and we found that the circadian rhythm of gas concentrations emitted from biosensors changes seasonally. The pyramid power and Bio-Entanglement did not change the number of cycles in the periodic approximation curve representing circadian rhythm. Therefore, in this paper we analyzed the influence of the pyramid power and Bio-Entanglement, i.e., their influence on the phase, amplitude, and correlation coefficient of the periodic approximation curve representing the circadian rhythm of emitted gas concentrations. The main results are as follows. 1) The pyramid power shifted the phase of the periodic approximation curve representing the circadian rhythm by 43 minutes. 2) The amplitude of the periodic approximation curve changed with the pyramid power and the Bio-Entanglement. The effect on the lower and upper sections of the biosensors stacked in two layers was different, with a tendency to increase the amplitude of the lower layer and decrease the amplitude of the upper layer. 3) The pyramid power and the Bio-Entanglement affected the correlation coefficient between gas concentration and the periodic approximation curve representing the circadian rhythm of gas concentration. The effect on the lower and upper layers of the biosensors was different, with a tendency for the lower layer correlation coefficient to be larger and the upper layer correlation coefficient to be smaller. Previously we demonstrated that the pyramid power and the Bio-Entanglement affect the ratio of gas concentration, i.e., psi index Ψ. In this paper we demonstrate for the first time that the pyramid power and the Bio-Entanglement affect time, i.e., phase difference.
文摘Exposure to toxins can lead to a wide range of adverse health effects, including respiratory problems, neurological disorders, cancer, and reproductive issues. Toxins can come from various sources, such as industrial waste, agricultural runoff, and household chemicals. Therefore, detecting and monitoring toxins in the environment is crucial for protecting human health and the environment. This study aimed to evaluate the performance of Hememics biosensor system in detecting environmental toxins such as Ricin and Staphylococcal enterotoxin B (SEB) in mixed matrixes. When Ricin and SEB are spiked into soil, chopped lettuce, tap water, milk and serum, the biosensor was able to detect these toxins, without sample processing, at a level of detection comparable to lab testing with high sensitivity and specificity. Furthermore, Hememics biosensor system is designed to be network-enabled, which means that results can be transmitted to relevant agencies for quick decisions. This feature is crucial in cases where quick action is needed to prevent further contamination or exposure to harmful toxins.
基金Pasteur Institute of Iran for supporting the current article
文摘Fast and precise diagnostic techniques are required for the treatment of many disorders.Biosensors are one of the diagnostic devices that are applicable in biological and medical sciences.Biosensors could be utilized to recognize biological molecules with high sensitivity.Biosensors are consisted of different components and have different types.Each type of biosensor is used in a particular field according to its specific features.Nanobodies are a novel class of antibodies with small size,high affinity,and specificity to their target.The unique properties of nanobodies make them appropriate tools for diagnostic applications.In this paper,we review biosensors,and their features and roles in medicine.Antibody/nanobody-based biosensors are also specifically discussed.
基金This work was supported by the Department of Science&Technology(DST)(Grant No.TDP/BDTD/33/2019)the Science and Engineering Research Board(SERB)(Grant Nos.EMR/2016/007564 and YSS/2015/000023)the Biotechnology Industry Research Assistance Council(BIRAC)(Grant No.BT/IIPME0211/02/16).
文摘Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applications of advanced nanomaterials in biosensors.Every year there are several advances in sensing techniques that can be attributed to nanomaterials,biorecognition elements,or their related fabrication techniques.The further development of nanotechnology-based sensors provides a wide variety of opportunities to modern research.Advanced nanomaterials can provide remarkable optical,electrical,mechanical,and catalytic properties.For example,transition metals and organic polymers have been used in the fabrication of powerful,sensitive,and precise biosensors.The distinctive properties of advanced nanomaterials have been widely incorporated into biosensors.However,fabrication techniques also play important roles in the development of these devices.Therefore,we present a review of some of the advanced nanomaterials that have been widely used over the last few years and discuss their fabrication techniques.The focus of this review is to provide a directional perspective of recently fabricated advanced nanomaterial-based biosensors in the diagnosis of various diseases.
基金Supported by National Natural Science Foundation of China,No.81371885
文摘A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical research,for example,the blood glucose biosensor.This review focuses on the current state of biosensor research with respect to efficient,specific and rapid detection of hepatitis B virus(HBV).The biosensors developed based on different techniques,including optical methods(e.g.,surface plasmon resonance),acoustic wave technologies(e.g.,quartz crystal microbalance),electrochemistry(amperometry,voltammetry and impedance) and novel nanotechnology,are also discussed.
基金Department of Science and Technology(DST)Government of India,for sanctioning financial assistance for executing this programme under Nanomaterials Science and Technology Initiative Programmethe Council of Scientific and Industrial Research(CSIR),Government of India,for granting her fellowship for executing this programme
文摘An amperometric hydrogen peroxide biosensor using a nanobiocomposite based on neutral red modified carbon nanotubes and co-immobilized glucose oxidase and horseradish peroxidase is reported. Modification of the nanobiocomposite electrode with neutral red resulted in a sensitive, low-cost and reliable H_2O_2 sensor. The use of carbon nanotubes, as the conductive part of the composite, facilitated fast electron transfer rates. The biosensor was characterized for the influence of p H, potential and temperature. A remarkable feature of the biosensor is the detection of H_2O_2 at low applied potentials where the noise level and interferences are minimal. The sensor has a fast steady-state measuring time of 10 s with a quick response(2 s). The biosensor showed a linear range from 15 n M to 45 m M of H_2O_2 and a detection limit of 5 n M. Nafion, which is used as a binder, makes the determination free from other electroactive substances. The repeatability, reproducibility,stability and analytical performance of the sensor are very good.
基金supported by grants from the National Key Research and Development Project(Project No.:2019YFC1605800)the National Natural Science Foundation of China(Grant Nos.:22006070 and 82103816)+2 种基金the Natural Science Foundation of Jiangsu Province(Grant Nos.:BK20200715,BK20200718,and BK20210538)the Natural Science Foundation of Jiangsu Higher Education Institutions,China(Grant No.:20KJB350010)the Natural Science Fund for Colleges and Universities in Jiangsu Province(Grant No.:19KJB530011).
文摘Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites pose a potential risk of allergic reactions,bacterial resistance,and increased cancer incidence.Residual antibiotics and the resulting bacterial antibiotic resistance have been recognized as a global challenge that has attracted increasing attention.Therefore,monitoring antibiotics is a critical way to limit the ecological risks from antibiotic pollution.Accordingly,it is desirable to devise new analytical platforms to achieve efficient antibiotic detection with excellent sensitivity and specificity.Quantum dots(QDs)are regarded as an ideal material for use in the development of antibiotic detection biosensors.In this review,we characterize different types of QDs,such as silicon,chalcogenide,carbon,and other doped QDs,and summarize the trends in QD-based antibiotic detection.QD-based sensing applications are classified according to their recognition strategies,including molecularly imprinted polymers(MIPs),aptamers,and immunosensors.We discuss the advantages of QD-derived antibiotic sensors,including low cost,good sensitivity,excellent stability,and fast response,and illustrate the current challenges in this field.
基金Fund supported by the National Natural Science Foundation of China (81770491) and the Ministry of Education Innovation TeamDevelopment Program of China (IRT16R57).
文摘Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery,biopharmaceutical manufacturing,gene therapy and tissue engineering,etc.In this review,we focus on current technologies of synthetic biosensors for disease detection.We start with the design principle of synthetic biosensors.Then we move towards the characteristics of simple synthetic biosensors,which can respond to a single input signal,and complex synthetic biosensors including Boolean gate biosensors,cascade biosensors,time-delay biosensors,oscillator biosensors and hysteretic biosensors,which can respond to more than two input signals and perform complex tasks.Synthetic biosensor has showed great potential in disease detection,but it is still in its infancy stage.More efforts should be made in identifying and constructing clinically relevant regulation systems.Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor.The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene-and cell-based therapies.
文摘A novel quartz crystal microbalance (QCM) biosensor for the determination of organophosphorus pesticide,such as dichlorvos (DDVP),has been fabricated with poly (3,4-ethylenedioxythiophene)/Poly (4-styrenesulfonate) (PEDOT/PSS) and cholinesterase butyl (BuChE).It is found that PEDOT/PSS coated sensor exhibits the highest sensitivity for DDVP among PEDOT,polyvinyl pyrrolidone,polyvinyl alcohol and polyacrylic acid sensor series.The conditions to prepare PEDOT/PSS coated sensor are optimized.The sensitivity of the PEDOT/PSS hiosensor with BuChE is higher than the others without BuChE The determination of dichlorvos in the range from 400μg/g to 2400μg/g used PEDOT/PSS with BuChE coated hiosensor was obtained.
基金the Fatih University,Research Project Foundation (Contract no:P500209022)Scientific and Technological Research Council of Turkey (TBTAK) (Pro ject no:110T487)TURKEY Prime Ministry State Planning Organization
文摘Nickel ferrite(NiFe_2O_4) nanoparticles have been dispersed in chitosan solution in order to fab ricate nanocomposite films.Horseradish peroxidase(HRP) has been immobilized onto this chitosan NiFe_2O_4 nanocomposite film via physical adsorption.The size of the NiFe_2O_4 nanoparticles has been estimated us ing X ray diffraction pattern and scanning electron microscopy(SEM) to be 40±9 nm.The chitosan NiFe_2O_4 nanocomposite film and HRP/chitosan NiFe_2O_4 bioelectrode have been characterized using SEM technique.The HRP/chitosan NiFe_2O_4 nanocomposite bioelectrode has a response time of 4 s,linearity as 0.3 to 12 m M of H2O2,sensitivity as 22 n A/m M.The effects of p H and the temperature of the immobilized HRP electrode have also been studied.
基金supported by the National Natural Science Foundation of China(No.30972055,31101286)Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China(No.2011GB2C60020)Shandong Provincial Natural Science Foundation,China(No.Q2008D03)
文摘In this paper, an amperometric acetylcholinesterase(ACh E) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly(diallyldimethy-lammonium chloride)-multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode(GCE) surface, then ACh E was immobilized onto the modified GCE to fabricate the ACh E biosensor. The morphologies and electrochemistry properties of the prepared ACh E biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the ACh E activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.
基金The financial support from the National Key Research and Development Program of China(2017YFC1600300).
文摘Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent feed contamination.Traditional detection methods can no longer meet the needs of massive,real-time,simple,and fast mycotoxin monitoring.Rapid detection methods based on advanced material and sensor technology are the future trend.In this review,we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods,especially for simultaneous multiplex mycotoxin determination.Immunoassays,biosensors,and the prominent roles of nanomaterials are introduced.The principles of different types of recognition and signal transduction are explained,and the merits and pitfalls of these methods are compared.Furthermore,limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.
