[ Objective ] The paper was to study the effects of several botanical pesticides against two pests in tea plantation. [ Method] Using randomized block de- sign, different concentrations of botanical pesticides were sp...[ Objective ] The paper was to study the effects of several botanical pesticides against two pests in tea plantation. [ Method] Using randomized block de- sign, different concentrations of botanical pesticides were sprayed during the young larvae periods of Ectropis oblique hmpulina Wehrli and peak periods of Empoasca pirisuga, the reduction rate of population and control effects were investigated after spraying for 1,3,7 and 10 d. [ Result] 5% rotenone EC 800 times had the best control effect against E. oblique hmpulina, and 0.5% veratridine soluble liquid 800 times had the best control effect against E. pirisuga. 0. 5% veratridine soluble liquid, 0.2% celangulin EC, 0.3% matrine water agent and 7.5% rotenone EC could be selected as the pesticides for the control of E. oblique hmpulina; 0. 5% veratridine soluble liquid, 0. 2% celangulin EC, 0.3% azadirachtin EC, 0.3% matrine water agent and 7.5% rotenone EC could be selected as the pesticides for the control of E. pirisuga, of which 0. 3% azadiraehtin EC 600 times showed the best durable effect against E. pirisuga with control effect of 100% after spraying for 7 d. [ Conclusion] The results provided basis for application of botanical pesticides in pests control in tea plantation.展开更多
Apolygus lucorum(Meyer-Dür.) and Erythroneura apicalis(Nawa) are important pests that affect the quality and the yield of grapevine and cause huge economic losses. This paper focuses on the selection of effec...Apolygus lucorum(Meyer-Dür.) and Erythroneura apicalis(Nawa) are important pests that affect the quality and the yield of grapevine and cause huge economic losses. This paper focuses on the selection of effective botanical pesticides to control A. lucorum and E. apicalis. This experiment explores the effect of several botanical pesticides for A. lucorum and E. apicalis, including the 0.5% veratrine, the0.6% Oxygen·Lactone agent, the 5% natural pyrethrin, the composite neem pesticide, the rotenone and the composite nicotine. The 0.5% veratrine shows a stable control efficacy, which is higher than 60% in Chengdu, while the composite nicotine shows the highest efficacy against A. lucorum, which is above 70%. In Yinchuan,the 0.5% veratrine shows the highest efficacy, against the first generation adults and the second generation larvae of E. apicalis, while the 5% natural pyrethrin shows 100% control efficacy against E. apicalis in Nanjiang. The 0.5% veratrine and the composite neem could be used as effective pesticides to control A. lucorum and the 5% natural pyrethrin can be used to control E. apicalis. They could be widely used in the production of pollution-free grapes.展开更多
[ Objective] The optimum extraction technology of antifungal ingredients from eight medicinal plants such as coptis root and clove, etc. and their antifungal effects on two species of plant pathogens were studied. [ M...[ Objective] The optimum extraction technology of antifungal ingredients from eight medicinal plants such as coptis root and clove, etc. and their antifungal effects on two species of plant pathogens were studied. [ Method] The effective ingredients of medicinal plants were extracted by water, ethanol and acetone. The minimum inhibitory concentration (MIC) and antifungal rate of extracts from medicinal plants were determined using plate diluting method. [Resets] The optimum medicinal plants and the extraction methods inhibited two plant pathogens were as follows: clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit Fusarium oxysporum Schl., MIC at that mo- ment reached 4 mg/ml. Clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit the pathogen of Cistanche deserticola, MIC at that moment reached 4 mg/ml. [ Conclusion] The antifungal effects of ethanol and acetone extracts on tested patho- gens were obviously superior than that of aqueous extracts.展开更多
The characteristics,research and application situation of botanical pesticides are briefly introduced. The research and application of botanical insecticides in control of tobacco pests are reviewed,and the existing p...The characteristics,research and application situation of botanical pesticides are briefly introduced. The research and application of botanical insecticides in control of tobacco pests are reviewed,and the existing problems,prospect in development and utilization of botanical insecticides are also discussed.展开更多
Aiming at the safety problems of botanical pesticides currently ignored, the paper analyzes the unsafe factors existing in botanical pesticides from the three aspects of active ingredients, inactive ingredients and un...Aiming at the safety problems of botanical pesticides currently ignored, the paper analyzes the unsafe factors existing in botanical pesticides from the three aspects of active ingredients, inactive ingredients and unregistered active ingredients, in order to provide a scientific reference for the safe use and effective su- pervision of botanical pesticides.展开更多
Common beans are affected by many insect pests such as bean leaf beetle, aphids, legume pod borer and bean beetles. Traditional and cultural practices such as site selection, crop rotation, intercropping and seed sele...Common beans are affected by many insect pests such as bean leaf beetle, aphids, legume pod borer and bean beetles. Traditional and cultural practices such as site selection, crop rotation, intercropping and seed selection, sowing date are used to reduce the infestation of insect pests of common beans in the field and in storage rooms. Natural enemies such as predators, parasitoids and pathogens can control the insect pests. Synthetic pesticides such as cypermethion, carbaryl, and lambda-cyhalothrin have reported to be effective, but are toxic to people, destroy natural enemies and contaminate the environment. Botanical pesticides are the promising alternatives. This review paper explains toxicity, persistence and mode of actions of active ingredients of botanical pesticides. Rotenone from T. vogelii has the oral lethal dose (LD<sub>50</sub>) of 132 - 1500 mg/kg to mammals. It delays the electron transport chain in mitochondria of the insects and limits the cellular energy production. Azadirachtin is antifeedant and growth disruptor of insects. It has low toxicity to mammals. The oral LD<sub>50</sub> in mammals is greater than 3540 mg/kg. Azadirachtin displays strong effects on chemoreceptors of the insects. Pyrethrins are axonic poisons and have repellent effects to insects. It is less toxic to mammals with the LD<sub>50</sub> of about 1500 mg/Kg. It attacks the nervous systems of insects. Sesquiterpene lactones from T. diversifolia, Pentacyclic triterpenoids from Lantana camara, Vernodalin, Vernodalol and Epivernodalol from V. amygdalina have repellent and feeding deterrents chemicals which discourage the insects from feeding the crop. Most active ingredients of botanical pesticides have short life span in the environment.展开更多
From the viewpoint of characteristics of botanical pesticides, the development and market prospect of botanical pesticides in China were analyzed, and the resources of botanical pesticides in Changbai Mountain, as wel...From the viewpoint of characteristics of botanical pesticides, the development and market prospect of botanical pesticides in China were analyzed, and the resources of botanical pesticides in Changbai Mountain, as well as corresponding for their development and utilization were summed up in this paper.展开更多
By screening emulsifiers and co-solvents and determining relevant physical and chemical properties, the preparation method for 16% Chijunning micreemulsion was studied in the paper. The best formulation (volume fract...By screening emulsifiers and co-solvents and determining relevant physical and chemical properties, the preparation method for 16% Chijunning micreemulsion was studied in the paper. The best formulation (volume fraction) was as follows: Rad/x phytolaccae extract 4%, Magnolia offtcinalis extract 4%, Xanthium sibiricum extract 8%, DBS-Ca 10%, pesticide emulsifier A 15%, cyclohexanone 20%, water 39%. The microemulsion had better development and market future because of its good stability, low price, use security and environmental protection.展开更多
[Objectives] The paper was to explore the control effects of different botanical pesticides against Empoasca pirisuga Matumura. [Methods] 5% Eucalyptol SL, 0.5% matrine SL and 50% thiacloprid WDG were used to control ...[Objectives] The paper was to explore the control effects of different botanical pesticides against Empoasca pirisuga Matumura. [Methods] 5% Eucalyptol SL, 0.5% matrine SL and 50% thiacloprid WDG were used to control E. pirisuga , and the decline rate of insect population was investigated. [Results] Eucalyptol had good control effect on E. pirisuga , with slow effect but long duration. It was harmless to natural enemies in tea gardens, with high safety and no chemical injury. [Conclusions] The study provides a theoretical basis for the application of botanical pesticides in tea production.展开更多
Traditional Chinese herbal medicines not only cure human diseases,but also play an important role as insecticides.Compared with conventional chemical agents,traditional Chinese herbal medicines are characterized by lo...Traditional Chinese herbal medicines not only cure human diseases,but also play an important role as insecticides.Compared with conventional chemical agents,traditional Chinese herbal medicines are characterized by low toxicity,low residues,and being eco-friendly,and they have become a research hotspot.Traditional Chinese herbal medicines have tremendous flexibility and indefinite potential.Therefore,this paper reviewed the types of insecticides belonging to traditional Chinese herbal medicines in Inner Mongolia,China,including their traditional uses,secondary metabolites,biological activities,action mechanisms,application methods,and development status.In addition,the most relevant issues involved in the development of traditional Chinese herbal medicines was discussed.We believe that traditional Chinese herbal medicines can be better implemented and developed;such that its other advantages,such as an insect repellent,can be promoted.Moreover,this study lays a solid foundation for further research on traditional Chinese herbal medicines in Inner Mongolia,China.展开更多
In order to provide the technological support for further implementing measures of reducing chemical pesticide to control plant diseases,the research progress on non-chemical pesticide measures to control plant diseas...In order to provide the technological support for further implementing measures of reducing chemical pesticide to control plant diseases,the research progress on non-chemical pesticide measures to control plant diseases are reviewed from the aspects of agricultural control,botanical pesticide control and microbial pesticide control,and the development prospects are proposed,including accelerating innovative research on botani-cal pesticide control such as Chinese herb extracts,and screening microbial pesticides from valuable bio-control bacteria or plant endophyte metabolites for commercial production and utilization.展开更多
The beet armyworm(BAW),Spodoptera exigua(Lepidoptera:Noctuidae)is a highly destructive pest of vegetables and field crops.Management of beet armyworm primarily relies on synthetic pesticides,which is threatening the b...The beet armyworm(BAW),Spodoptera exigua(Lepidoptera:Noctuidae)is a highly destructive pest of vegetables and field crops.Management of beet armyworm primarily relies on synthetic pesticides,which is threatening the beneficial community and environment.Most importantly,the BAW developed resistance to synthetic pesticides with making it difficult to manage.Therefore,alternative and environment-friendly pest management tactics are urgently required.The use of pesticidal plant extracts provides an effective way for a sustainable pest management program.To evaluate the use of pesticidal plant extracts against BAW,we selected six plant species(Lantana camara,Aloe vera,Azadirachta indica,Cymbopogon citratus,Nicotiana tabacum,and Ocimum basilicum)for initial screening experiment.Four out of six plant species such as A.indica,N.tabacum,C.citratus and O.basilicum showed promising mortality of more than 50%.Therefore,we selected these four plant extracts for the subsequent experiments.Through contact bioassay,A.indica showed high mortality 66.63%,followed by the N.tabacum 53.33%,at 10%w/v concentration.Similarly,N.tabacum showed the highest mortality rate,66%at 10%w/v concentration,followed by the A.indica 46%through feeding bioassay.Furthermore,the feeding deterrence assay showed that C.citratus had a high antifeedant index(−50)followed by A.indica(−39),and N.tabacum(−28).In living plant assay,the N.tabacum extract showed a low mean damage score 3.6 on living cotton plant followed by C.citratus 4.5 and A.indica 5.5.Hence,extracts of three plant species provided promising results against the BAW,which can minimize the use of synthetic chemicals,particularly for small landholding farmers.Further studies are also required to evaluate the effects of these plant extract against BAW on cotton plants under field conditions to optimize the further use.展开更多
Twenty-four provenances of two species (Azadirachta siamensis and Azadirachta indica) have been introduced to China from South Asia, Southeast Asia and Africa Since 1995. This paper summarizes the researches on the ...Twenty-four provenances of two species (Azadirachta siamensis and Azadirachta indica) have been introduced to China from South Asia, Southeast Asia and Africa Since 1995. This paper summarizes the researches on the introduction and planting of Azadirachta indica and analyzes the morphological, phenological characteristics, the growth rhythm, pollinating and seed yielding features of the intro- duced 24 provenances of the two species as well as the variations of filial generation plants. The experiments showed that most of the prove- nances of,4. indica have normal growth and can blossom and fruit in the dry-hot valleys with tropical climate conditions in Yunnan Province, China. The normal regions for A. indica were classified and the selection criteria for superior plants were put forward in this paper, more- over, the major contents of industry planning and technical approaches for A. indica plantation establishment were discussed and the countermeasures to reduce the neem-based pesticide products were also proposed.展开更多
[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. ...[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. vespertilio, the insecticidal effect of water extract of oleander against the larvae was studied. [Result] The 1/20-1/200 times water extract of dry leaf weight of oleander had better insecticidal effect against the larvae of E. vespertilio, and the feeding mortality rate reached 100%. [Conclusion] The paper provided reference for development of new botanical pesticides with high efficiency, environmental protection and low carbon.展开更多
[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment fr...[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum (stem and leaf), Cryptomeriafortunei (stem and leaf), Pinus yunnanensis (stem), Pinus yunnanensis (leaf), Rhododendron simsii (leaf), Pinus armandii (leaf), Litchi chinensis (pericarp), P/nus armandiifruit (fruit) and Alnus cremastogyne (leaf) showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum (stem and leaf) was the strongest, reaching(1068.42 +7.87)%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum (stem and leaf), C. fortunei (stem and leaf), P. yunnanens/s (stem), P. yurmanens/s (leaf), R. simsii (leaf), P. armandii (leaf), L. chinensis (pericarp), P. armandiifruit (fruit) and A. cremastogyne (leaf) all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.展开更多
Root-Knot Nematodes (RKN) Meloidogyne spp. significantly affects glasshouse tomatoes in Central Greece. Elimination or halting J2s could be an efficient strategy to control RKN. Recently, it was reported that a comm...Root-Knot Nematodes (RKN) Meloidogyne spp. significantly affects glasshouse tomatoes in Central Greece. Elimination or halting J2s could be an efficient strategy to control RKN. Recently, it was reported that a commercial product of Neem (Azadirachta indica) seed extract (Azadirachtin 1% Emulsion Concentrate-EC) significantly reduced the number of galls on tomato roots and egg masses, compared to the untreated control. In the present study neem proved to have a direct immobilising effect on RKN J2s. Different concentrations (0, 0.05, 0,1, 0.25, 0.5, I, 5, 10, 25 and 50%) of the tested neem product were applied with water containing fresh J2s and in soil naturally infected with RKN. Records of immobilised J2s were taken after 10 min, 1 h, 6 h, 24 h and 72 h of Neem application in water and after six days of neem application into the soil. Neem solution concentrations 5% and 10%, resulted in 85% ± 3.2 and 100% of immobilised J2s. The same concentrations of neem when were added in the water for nematode extraction from the soil, resulted 14.25% ± 4.01 and 1.7%, of J2s extracted from the soil with a significant concentration effect (P = 0.008), compared to the untreated control. However, the neem product at high application dose significant damage the tomato plants caused phytoxicity.展开更多
[Objectives] The paper was to study the prevention and control effect of botanical pesticides on Semiaphis heraclei at different temperatures. [Methods] When the daily average temperatures in spring were 15 and 20 ℃,...[Objectives] The paper was to study the prevention and control effect of botanical pesticides on Semiaphis heraclei at different temperatures. [Methods] When the daily average temperatures in spring were 15 and 20 ℃, the control effects of 0.3% azadirachtin EC 500 times dilution and 0.3% matrine AS 1 000 times dilution on S. heraclei were studied. [Results] When the daily average temperature was 15 ℃, 0.3% azadirachtin EC 500 times dilution had relatively good control effect on S. heraclei, with a long duration. The prevention and control of S. heraclei in spring better controlled the population quantity of S. heraclei fundatrix and reduced the degree of harm. [Conclusions] The study provides a basis for the pollution-free control of S. heraclei in the green space of parks.展开更多
[Objective]The paper was to screen the plant extracts with strong antifungal activity. [Method] With R. solani as the test object, the antifungal activities of 30 kinds of plant extracts were carried out primary scree...[Objective]The paper was to screen the plant extracts with strong antifungal activity. [Method] With R. solani as the test object, the antifungal activities of 30 kinds of plant extracts were carried out primary screening by mycelial growth rate method. [Result] Twelve kinds of plant extracts including Pinus yunnanensis (leaf), Phryma leptostachya (stem and leaf), Pueraria peduncularis (root, stem and leaf), Pinus armandiifruit (fruit), Aconitum brachypodum (stem and leaf), Phellodendron amurense (stem and leaf), Rhaponticum uniflorum (root), Alnus cremastogyne (leaf), Rehmannia glutinosa (root), Cryptomeria fortunei (stem and leaf) and Berberis pruinosa (stem and leaf) had strong activities against R. solani with growth inhibition rates over 80% as the concentration was 10 mg/ml,among which the extracts of P. yunnanensis (leaf), P. leptostachya (stem and leaf), P. peduncularis (root, stem and leaf) and P. armandiifruit (fruit) showed the inhibition rate above 95%, significantly higher than other plant extracts. The secondary screening results of antifungal activities of the above 12 plant extracts were consistent with the primary screening. [Conclusion] The study laid foundation for further development of high efficient, safe and environmental-friendly new fungicides.展开更多
The establishment of suspension culture system for neem (Azadirachta indica A. Juss) cells and the suspension culture condition was studied. It shows that the neem cell suspension culture system was best in B5 liquid ...The establishment of suspension culture system for neem (Azadirachta indica A. Juss) cells and the suspension culture condition was studied. It shows that the neem cell suspension culture system was best in B5 liquid medium, 2.0~4.0mg/L NAA with direct spill method. Based on the integrated analysis of cell biomass, Azadirachtin content and productivity, the optimum culture conditions were B5 liquid medium, 2.0-4.0 mg/L NAA, 3% sucrose at 25 ℃. The optimum rotating speed of the shaker and broth content d...展开更多
基金Supported by Key Agricultural Scientific and Technological Project of Henan Province(102102110119)~~
文摘[ Objective ] The paper was to study the effects of several botanical pesticides against two pests in tea plantation. [ Method] Using randomized block de- sign, different concentrations of botanical pesticides were sprayed during the young larvae periods of Ectropis oblique hmpulina Wehrli and peak periods of Empoasca pirisuga, the reduction rate of population and control effects were investigated after spraying for 1,3,7 and 10 d. [ Result] 5% rotenone EC 800 times had the best control effect against E. oblique hmpulina, and 0.5% veratridine soluble liquid 800 times had the best control effect against E. pirisuga. 0. 5% veratridine soluble liquid, 0.2% celangulin EC, 0.3% matrine water agent and 7.5% rotenone EC could be selected as the pesticides for the control of E. oblique hmpulina; 0. 5% veratridine soluble liquid, 0. 2% celangulin EC, 0.3% azadirachtin EC, 0.3% matrine water agent and 7.5% rotenone EC could be selected as the pesticides for the control of E. pirisuga, of which 0. 3% azadiraehtin EC 600 times showed the best durable effect against E. pirisuga with control effect of 100% after spraying for 7 d. [ Conclusion] The results provided basis for application of botanical pesticides in pests control in tea plantation.
基金Supported by the Special Program for the Construction of Modern Agricultural Industrial Technological System(CARS-30-bc)~~
文摘Apolygus lucorum(Meyer-Dür.) and Erythroneura apicalis(Nawa) are important pests that affect the quality and the yield of grapevine and cause huge economic losses. This paper focuses on the selection of effective botanical pesticides to control A. lucorum and E. apicalis. This experiment explores the effect of several botanical pesticides for A. lucorum and E. apicalis, including the 0.5% veratrine, the0.6% Oxygen·Lactone agent, the 5% natural pyrethrin, the composite neem pesticide, the rotenone and the composite nicotine. The 0.5% veratrine shows a stable control efficacy, which is higher than 60% in Chengdu, while the composite nicotine shows the highest efficacy against A. lucorum, which is above 70%. In Yinchuan,the 0.5% veratrine shows the highest efficacy, against the first generation adults and the second generation larvae of E. apicalis, while the 5% natural pyrethrin shows 100% control efficacy against E. apicalis in Nanjiang. The 0.5% veratrine and the composite neem could be used as effective pesticides to control A. lucorum and the 5% natural pyrethrin can be used to control E. apicalis. They could be widely used in the production of pollution-free grapes.
基金Supported by Shanxi Science and Technology Department Project(041020)~~
文摘[ Objective] The optimum extraction technology of antifungal ingredients from eight medicinal plants such as coptis root and clove, etc. and their antifungal effects on two species of plant pathogens were studied. [ Method] The effective ingredients of medicinal plants were extracted by water, ethanol and acetone. The minimum inhibitory concentration (MIC) and antifungal rate of extracts from medicinal plants were determined using plate diluting method. [Resets] The optimum medicinal plants and the extraction methods inhibited two plant pathogens were as follows: clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit Fusarium oxysporum Schl., MIC at that mo- ment reached 4 mg/ml. Clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit the pathogen of Cistanche deserticola, MIC at that moment reached 4 mg/ml. [ Conclusion] The antifungal effects of ethanol and acetone extracts on tested patho- gens were obviously superior than that of aqueous extracts.
文摘The characteristics,research and application situation of botanical pesticides are briefly introduced. The research and application of botanical insecticides in control of tobacco pests are reviewed,and the existing problems,prospect in development and utilization of botanical insecticides are also discussed.
基金Supported by National Innovation Project(2009IM032500)Science and Technology Supporting Program of Sichuan Province(2009NZ0015)
文摘Aiming at the safety problems of botanical pesticides currently ignored, the paper analyzes the unsafe factors existing in botanical pesticides from the three aspects of active ingredients, inactive ingredients and unregistered active ingredients, in order to provide a scientific reference for the safe use and effective su- pervision of botanical pesticides.
文摘Common beans are affected by many insect pests such as bean leaf beetle, aphids, legume pod borer and bean beetles. Traditional and cultural practices such as site selection, crop rotation, intercropping and seed selection, sowing date are used to reduce the infestation of insect pests of common beans in the field and in storage rooms. Natural enemies such as predators, parasitoids and pathogens can control the insect pests. Synthetic pesticides such as cypermethion, carbaryl, and lambda-cyhalothrin have reported to be effective, but are toxic to people, destroy natural enemies and contaminate the environment. Botanical pesticides are the promising alternatives. This review paper explains toxicity, persistence and mode of actions of active ingredients of botanical pesticides. Rotenone from T. vogelii has the oral lethal dose (LD<sub>50</sub>) of 132 - 1500 mg/kg to mammals. It delays the electron transport chain in mitochondria of the insects and limits the cellular energy production. Azadirachtin is antifeedant and growth disruptor of insects. It has low toxicity to mammals. The oral LD<sub>50</sub> in mammals is greater than 3540 mg/kg. Azadirachtin displays strong effects on chemoreceptors of the insects. Pyrethrins are axonic poisons and have repellent effects to insects. It is less toxic to mammals with the LD<sub>50</sub> of about 1500 mg/Kg. It attacks the nervous systems of insects. Sesquiterpene lactones from T. diversifolia, Pentacyclic triterpenoids from Lantana camara, Vernodalin, Vernodalol and Epivernodalol from V. amygdalina have repellent and feeding deterrents chemicals which discourage the insects from feeding the crop. Most active ingredients of botanical pesticides have short life span in the environment.
文摘From the viewpoint of characteristics of botanical pesticides, the development and market prospect of botanical pesticides in China were analyzed, and the resources of botanical pesticides in Changbai Mountain, as well as corresponding for their development and utilization were summed up in this paper.
文摘By screening emulsifiers and co-solvents and determining relevant physical and chemical properties, the preparation method for 16% Chijunning micreemulsion was studied in the paper. The best formulation (volume fraction) was as follows: Rad/x phytolaccae extract 4%, Magnolia offtcinalis extract 4%, Xanthium sibiricum extract 8%, DBS-Ca 10%, pesticide emulsifier A 15%, cyclohexanone 20%, water 39%. The microemulsion had better development and market future because of its good stability, low price, use security and environmental protection.
基金Supported by National Key Research and Development Program of China (2016YFD0200900)China Agriculture Research System of MOF and MARA (CARS-19).
文摘[Objectives] The paper was to explore the control effects of different botanical pesticides against Empoasca pirisuga Matumura. [Methods] 5% Eucalyptol SL, 0.5% matrine SL and 50% thiacloprid WDG were used to control E. pirisuga , and the decline rate of insect population was investigated. [Results] Eucalyptol had good control effect on E. pirisuga , with slow effect but long duration. It was harmless to natural enemies in tea gardens, with high safety and no chemical injury. [Conclusions] The study provides a theoretical basis for the application of botanical pesticides in tea production.
基金funded by the National Natural Science Foundation of China(No.M2042003,M1942003)the Science and Technology Planning Project of the Inner Mongolia Autonomous Region,China(No.2021GG0377)+1 种基金Science and Technology Program in Inner Mongolia(No.2020GG0144)China Agriculture Research System of MOF and MARA(No.CARS-21).
文摘Traditional Chinese herbal medicines not only cure human diseases,but also play an important role as insecticides.Compared with conventional chemical agents,traditional Chinese herbal medicines are characterized by low toxicity,low residues,and being eco-friendly,and they have become a research hotspot.Traditional Chinese herbal medicines have tremendous flexibility and indefinite potential.Therefore,this paper reviewed the types of insecticides belonging to traditional Chinese herbal medicines in Inner Mongolia,China,including their traditional uses,secondary metabolites,biological activities,action mechanisms,application methods,and development status.In addition,the most relevant issues involved in the development of traditional Chinese herbal medicines was discussed.We believe that traditional Chinese herbal medicines can be better implemented and developed;such that its other advantages,such as an insect repellent,can be promoted.Moreover,this study lays a solid foundation for further research on traditional Chinese herbal medicines in Inner Mongolia,China.
基金Supported by Open Fund Project of Key Laboratory of Plant Nutrition and Fertilizer,Ministry of Agriculture and Rural Affairs"Study on Precise Nutrient Demand Model of Corn"(KLPNF-2018-4)
文摘In order to provide the technological support for further implementing measures of reducing chemical pesticide to control plant diseases,the research progress on non-chemical pesticide measures to control plant diseases are reviewed from the aspects of agricultural control,botanical pesticide control and microbial pesticide control,and the development prospects are proposed,including accelerating innovative research on botani-cal pesticide control such as Chinese herb extracts,and screening microbial pesticides from valuable bio-control bacteria or plant endophyte metabolites for commercial production and utilization.
文摘The beet armyworm(BAW),Spodoptera exigua(Lepidoptera:Noctuidae)is a highly destructive pest of vegetables and field crops.Management of beet armyworm primarily relies on synthetic pesticides,which is threatening the beneficial community and environment.Most importantly,the BAW developed resistance to synthetic pesticides with making it difficult to manage.Therefore,alternative and environment-friendly pest management tactics are urgently required.The use of pesticidal plant extracts provides an effective way for a sustainable pest management program.To evaluate the use of pesticidal plant extracts against BAW,we selected six plant species(Lantana camara,Aloe vera,Azadirachta indica,Cymbopogon citratus,Nicotiana tabacum,and Ocimum basilicum)for initial screening experiment.Four out of six plant species such as A.indica,N.tabacum,C.citratus and O.basilicum showed promising mortality of more than 50%.Therefore,we selected these four plant extracts for the subsequent experiments.Through contact bioassay,A.indica showed high mortality 66.63%,followed by the N.tabacum 53.33%,at 10%w/v concentration.Similarly,N.tabacum showed the highest mortality rate,66%at 10%w/v concentration,followed by the A.indica 46%through feeding bioassay.Furthermore,the feeding deterrence assay showed that C.citratus had a high antifeedant index(−50)followed by A.indica(−39),and N.tabacum(−28).In living plant assay,the N.tabacum extract showed a low mean damage score 3.6 on living cotton plant followed by C.citratus 4.5 and A.indica 5.5.Hence,extracts of three plant species provided promising results against the BAW,which can minimize the use of synthetic chemicals,particularly for small landholding farmers.Further studies are also required to evaluate the effects of these plant extract against BAW on cotton plants under field conditions to optimize the further use.
基金National Science & Technology Supporting Project (2006BAD18B03)Science & Technology Plan Projects of Yunnan province (2005YX20)
文摘Twenty-four provenances of two species (Azadirachta siamensis and Azadirachta indica) have been introduced to China from South Asia, Southeast Asia and Africa Since 1995. This paper summarizes the researches on the introduction and planting of Azadirachta indica and analyzes the morphological, phenological characteristics, the growth rhythm, pollinating and seed yielding features of the intro- duced 24 provenances of the two species as well as the variations of filial generation plants. The experiments showed that most of the prove- nances of,4. indica have normal growth and can blossom and fruit in the dry-hot valleys with tropical climate conditions in Yunnan Province, China. The normal regions for A. indica were classified and the selection criteria for superior plants were put forward in this paper, more- over, the major contents of industry planning and technical approaches for A. indica plantation establishment were discussed and the countermeasures to reduce the neem-based pesticide products were also proposed.
基金Supported by Innovation Experiment Program of Jiangsu Provincial College Students(5731506510)
文摘[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. vespertilio, the insecticidal effect of water extract of oleander against the larvae was studied. [Result] The 1/20-1/200 times water extract of dry leaf weight of oleander had better insecticidal effect against the larvae of E. vespertilio, and the feeding mortality rate reached 100%. [Conclusion] The paper provided reference for development of new botanical pesticides with high efficiency, environmental protection and low carbon.
基金Supported by The Natural Science Foundation Project of Yunnan Province ( No. 2010CD056)
文摘[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum (stem and leaf), Cryptomeriafortunei (stem and leaf), Pinus yunnanensis (stem), Pinus yunnanensis (leaf), Rhododendron simsii (leaf), Pinus armandii (leaf), Litchi chinensis (pericarp), P/nus armandiifruit (fruit) and Alnus cremastogyne (leaf) showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum (stem and leaf) was the strongest, reaching(1068.42 +7.87)%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum (stem and leaf), C. fortunei (stem and leaf), P. yunnanens/s (stem), P. yurmanens/s (leaf), R. simsii (leaf), P. armandii (leaf), L. chinensis (pericarp), P. armandiifruit (fruit) and A. cremastogyne (leaf) all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.
文摘Root-Knot Nematodes (RKN) Meloidogyne spp. significantly affects glasshouse tomatoes in Central Greece. Elimination or halting J2s could be an efficient strategy to control RKN. Recently, it was reported that a commercial product of Neem (Azadirachta indica) seed extract (Azadirachtin 1% Emulsion Concentrate-EC) significantly reduced the number of galls on tomato roots and egg masses, compared to the untreated control. In the present study neem proved to have a direct immobilising effect on RKN J2s. Different concentrations (0, 0.05, 0,1, 0.25, 0.5, I, 5, 10, 25 and 50%) of the tested neem product were applied with water containing fresh J2s and in soil naturally infected with RKN. Records of immobilised J2s were taken after 10 min, 1 h, 6 h, 24 h and 72 h of Neem application in water and after six days of neem application into the soil. Neem solution concentrations 5% and 10%, resulted in 85% ± 3.2 and 100% of immobilised J2s. The same concentrations of neem when were added in the water for nematode extraction from the soil, resulted 14.25% ± 4.01 and 1.7%, of J2s extracted from the soil with a significant concentration effect (P = 0.008), compared to the untreated control. However, the neem product at high application dose significant damage the tomato plants caused phytoxicity.
基金Supported by Construction Science and Technology Research Project of Hebei Province (2019-2072)。
文摘[Objectives] The paper was to study the prevention and control effect of botanical pesticides on Semiaphis heraclei at different temperatures. [Methods] When the daily average temperatures in spring were 15 and 20 ℃, the control effects of 0.3% azadirachtin EC 500 times dilution and 0.3% matrine AS 1 000 times dilution on S. heraclei were studied. [Results] When the daily average temperature was 15 ℃, 0.3% azadirachtin EC 500 times dilution had relatively good control effect on S. heraclei, with a long duration. The prevention and control of S. heraclei in spring better controlled the population quantity of S. heraclei fundatrix and reduced the degree of harm. [Conclusions] The study provides a basis for the pollution-free control of S. heraclei in the green space of parks.
基金Supported by Youth Fund Project of Yunnan Agricultural University
文摘[Objective]The paper was to screen the plant extracts with strong antifungal activity. [Method] With R. solani as the test object, the antifungal activities of 30 kinds of plant extracts were carried out primary screening by mycelial growth rate method. [Result] Twelve kinds of plant extracts including Pinus yunnanensis (leaf), Phryma leptostachya (stem and leaf), Pueraria peduncularis (root, stem and leaf), Pinus armandiifruit (fruit), Aconitum brachypodum (stem and leaf), Phellodendron amurense (stem and leaf), Rhaponticum uniflorum (root), Alnus cremastogyne (leaf), Rehmannia glutinosa (root), Cryptomeria fortunei (stem and leaf) and Berberis pruinosa (stem and leaf) had strong activities against R. solani with growth inhibition rates over 80% as the concentration was 10 mg/ml,among which the extracts of P. yunnanensis (leaf), P. leptostachya (stem and leaf), P. peduncularis (root, stem and leaf) and P. armandiifruit (fruit) showed the inhibition rate above 95%, significantly higher than other plant extracts. The secondary screening results of antifungal activities of the above 12 plant extracts were consistent with the primary screening. [Conclusion] The study laid foundation for further development of high efficient, safe and environmental-friendly new fungicides.
文摘The establishment of suspension culture system for neem (Azadirachta indica A. Juss) cells and the suspension culture condition was studied. It shows that the neem cell suspension culture system was best in B5 liquid medium, 2.0~4.0mg/L NAA with direct spill method. Based on the integrated analysis of cell biomass, Azadirachtin content and productivity, the optimum culture conditions were B5 liquid medium, 2.0-4.0 mg/L NAA, 3% sucrose at 25 ℃. The optimum rotating speed of the shaker and broth content d...