Calcium phosphate cements (CPC) are currently widely used bone replacement materials with excellent bioactivity, but have considerable disadvantages like slow degradation. For critical-sized defects, however, an impro...Calcium phosphate cements (CPC) are currently widely used bone replacement materials with excellent bioactivity, but have considerable disadvantages like slow degradation. For critical-sized defects, however, an improved degradation is essential to match the tissue regeneration, especially in younger patients who are still growing. We demonstrate that a combination of CPC with mesoporous bioactive glass (MBG) particles led to an enhanced degradation in vitro and in a critical alveolar cleft defect in rats. Additionally, to support new bone formation the MBG was functionalized with hypoxia conditioned medium (HCM) derived from rat bone marrow stromal cells. HCM-functionalized scaffolds showed an improved cell proliferation and the highest formation of new bone volume. This highly flexible material system together with the drug delivery capacity is adaptable to patient specific needs and has great potential for clinical translation.展开更多
Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects....Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects.展开更多
With the increase of global population,people’s life expectancy is growing as well.Humans tend to live more active lifestyles and,therefore,trauma generated large defects become more common.Instances of tumour resect...With the increase of global population,people’s life expectancy is growing as well.Humans tend to live more active lifestyles and,therefore,trauma generated large defects become more common.Instances of tumour resection or pathological conditions and complex orthopaedic issues occur more frequently increasing necessity for bone substitutes.Composition of calcium phosphate cements(CPCs)is comparable to the chemical structure of bone minerals.Their ability to self-set and resorb in vivo secures a variety of potential applications in bone regeneration.Despite the years-long research and several products already reaching the market,finding the right properties for calcium phosphate cement to be osteoinductive and both injectable and suitable for clinical use is still a sudoku.This article is focused on injectable,porous CPCs,reviewing the latest developments on the path toward finding osteoinductive material,which is suitable for injection.展开更多
Calcium phosphate cements(CPCs)have been widely used as bone graft substitutes for many years.The aim of this study was to evaluate the biocompatibility of two novel injectable,bioactive cements:b-tricalcium phosphate...Calcium phosphate cements(CPCs)have been widely used as bone graft substitutes for many years.The aim of this study was to evaluate the biocompatibility of two novel injectable,bioactive cements:b-tricalcium phosphate(b-TCP)/CPC and chitosan microsphere/CPC in vitro and in vivo.This was accomplished by culturing mouse pre-osteoblastic cells(MC3T3-E1)on discs and pastes of CPCs.Cell growth,adhesion,proliferation and differentiation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and alkaline phosphatase assays as well as by scanning electron microscopy and fluorescence.The effect of CPC paste curing was also evaluated.Implantation of two materials into the muscle tissue of rabbits was also studied and evaluated by histological analysis.Cell analysis indicated good biocompatibility in vitro.The fluorescence assay suggested that the cured material discs had no obvious effect on cell growth,while the curing process did.Histological examination showed no inflammatory cell infiltration into soft tissue.These data suggest that b-TCP/CPC and chitosan microsphere/CPC composites may be promising injectable material for bone tissue engineering.展开更多
Calcium phosphate cements are of great interest for researchers and their applications in medical practice expanded.Nevertheless,they have a number of drawbacks including the insufficient level of mechanical propertie...Calcium phosphate cements are of great interest for researchers and their applications in medical practice expanded.Nevertheless,they have a number of drawbacks including the insufficient level of mechanical properties and low degradation rate.Struvite(MgNH4PO4)-based cements,which grew in popularity in recent years,despite their neutral pH and acceptable mechanical performance,release undesirable NH4+ions during their resorption.This issue could be avoided by replacement of ammonia ions in the cement liquid with sodium,however,such cements have a pH values of 9–10,leading to cytotoxicity.Thus,the main goal of this investigation is to optimize the composition of cements to achieve the combination of desirable properties:neutral pH,sufficient mechanical properties,and the absence of cytotoxicity,applying Na2HPO4-based cement liquid.For this purpose,cement powders precursors in the CaO-MgO-P2O5 system were synthesized by one-pot process in a wide composition range,and their properties were investigated.The optimal performance was observed for the cements with(Ca+Mg)/P ratio of 1.67,which are characterized by newberyite phase formation during setting reaction,pH values close to 7,sufficient compressive strength up to 22±3 MPa(for 20 mol.%of Mg),dense microstructure and adequate matrix properties of the surface.This set of features make those materials promising candidates for medical applications.展开更多
The utilization of Calcium Phosphate Cement(CPC)is limited due to its low mechanical strength and difficulty to seed cells deep into the scaffold.The objectives of this study were to:(1)develop a 3D-printed CPC-dopami...The utilization of Calcium Phosphate Cement(CPC)is limited due to its low mechanical strength and difficulty to seed cells deep into the scaffold.The objectives of this study were to:(1)develop a 3D-printed CPC-dopamine-metformin scaffold encapsulating human periodontal ligament stem cells(hPDLSCs),(2)investigate the effect of dopamine on the performance of CPC,and(3)evaluate the effect of microbead degradation and metformin release on the osteogenic differentiation of the released hPDLSCs.The mechanical property of the CPC scaffolds was elevated by adding dopamine,and the CPC scaffold with 7 wt.%dopamine had the highest compressive strength(7.35 MPa).Four types of microbeads with different content of alginate(oxidized alginate),hPDLSCs,and 2%metformin were fabricated.Morphological and cell counting kit tests confirm that the hPDLSCs are protected by microbeads encapsulation during the CPC setting process.The alkaline phosphatase test indicates that the osteogenic differentiation of hPDLSCs was enhanced by the fast release of cells and metformin.The microbeads consisting of 2%oxidized alginate and 2%metformin were optimal for cell delivery due to favorable cell release and osteogenic differentiation.This CPC scaffold is promising used for bone regeneration in dental,craniofacial,and orthopedic applications.展开更多
Thanks to their biocompatibility,biodegradability,injectability and self-setting properties,calcium phosphate cements(CPCs)have been the most economical and effective biomaterials of choice for use as bone void filler...Thanks to their biocompatibility,biodegradability,injectability and self-setting properties,calcium phosphate cements(CPCs)have been the most economical and effective biomaterials of choice for use as bone void fillers.They have also been extensively used as drug delivery carriers owing to their ability to provide for a steady release of various organic molecules aiding the regeneration of defective bone,including primarily antibiotics and growth factors.This review provides a systematic compilation of studies that reported on the controlled release of drugs from CPCs in the last 25 years.The chemical,compositional and microstructural characteristics of these systems through which the control of the release rates and mechanisms could be achieved have been discussed.In doing so,the effects of(i)the chemistry of the matrix,(ii)porosity,(iii)additives,(iv)drug types,(v)drug concentrations,(vi)drug loading methods and(vii)release media have been distinguished and discussed individually.Kinetic specificities of in vivo release of drugs from CPCs have been reviewed,too.Understanding the kinetic and mechanistic correlations between the CPC properties and the drug release is a prerequisite for the design of bone void fillers with drug release profiles precisely tailored to the application area and the clinical picture.The goal of this review has been to shed light on these fundamental correlations.展开更多
Out of the wide range of calcium phosphate(CaP)biomaterials,calcium phosphate bone cements(CPCs)have attracted increased attention since their discovery in the 1980s due to their valuable properties such as bioactivit...Out of the wide range of calcium phosphate(CaP)biomaterials,calcium phosphate bone cements(CPCs)have attracted increased attention since their discovery in the 1980s due to their valuable properties such as bioactivity,osteoconductivity,injectability,hardening ability through a low-temperature setting reaction and moldability.Thereafter numerous researches have been performed to enhance the properties of CPCs.Nonetheless,low mechanical performance of CPCs limits their clinical application in load bearing regions of bone.Also,the in vivo resorption and replacement of CPC with new bone tissue is still controversial,thus further improvements of high clinical importance are required.Bioactive glasses(BGs)are biocompatible and able to bond to bone,stimulating new bone growth while dissolving over time.In the last decades extensive research has been performed analyzing the role of BGs in combination with different CaPs.Thus,the focal point of this review paper is to summarize the available research data on how injectable CPC properties could be improved or affected by the addition of BG as a secondary powder phase.It was found that despite the variances of setting time and compressive strength results,desirable injectable properties of bone cements can be achieved by the inclusion of BGs into CPCs.The published data also revealed that the degradation rate of CPCs is significantly improved by BG addition.Moreover,the presence of BG in CPCs improves the in vitro osteogenic differentiation and cell response as well as the tissue-material interaction in vivo.展开更多
Injectable materials show their special merits in regeneration of damaged/degenerated bones in minimally-invasive approach.Injectable calcium phosphate bone cement(CPC)has attracted broad attention for its bioactivity...Injectable materials show their special merits in regeneration of damaged/degenerated bones in minimally-invasive approach.Injectable calcium phosphate bone cement(CPC)has attracted broad attention for its bioactivity,as compared to non-degradable polymethyl methacrylate cement.However,its brittleness,poor anti-washout property and uncontrollable biodegradability are the main challenges to limit its further clinical application mainly because of its stone-like dense structure and fragile inorganic-salt weakness.Herein,we developed a kind of injectable CPC bone cement with porous structure and improved robustness by incorporating poly(lactide-co-glycolic acid)(PLGA)nanofiber into CPC,with carboxymethyl cellulose(CMC)to offer good injectability as well as anti-wash-out capacity.Furthermore,the introduction of PLGA and CMC also enabled a formation of initial porous structure in the cements,where PLGA nanofiber endowed the cement with a dynamically controllable biodegradability which provided room for cell movement and bone ingrowth.Inter-estingly,the reinforced biodegradable cement afforded a sustainable provision of Ca^(2+)bioactive components,together with its porous structure,to improve synergistically new bone formation and osteo-integration in vivo by using a rat model of femur condyle defect.Further study on regenerative mechanisms indicated that the good minimally-invasive bone regeneration may come from the synergistic enhanced osteogenic effect of calcium ion enrichment and the improved revascularization capacity contributed from the porosity as well as the lactic acid released from PLGA nanofiber.These results indicate the injectable bone cement with high strength,anti-washout property and controllable biodegradability is a promising candidate for bone regeneration in a minimally-invasive approach.展开更多
The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphat...The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphate cement(CPC)scaffolds combined with goat bone marrow stromal cells(BMSCs)were used to fill goat sinus floor space after maxillary sinus floor elevation with simultaneous implant placement comparing with those not filled any grafted materials and used as controls.After a healing period of 3 months,the goat maxillary sinus membrane was examined using light microscopy and scanning electronic microscopy.The results showed that the connective tissue thickness and the epithelium thickness of mucosa were not statistically significant difference between two groups.The tissue engineered bone complex might be an ideal graft for the sinus floor elevation and have no influence on the sinus membrane under the histological and ultrastructural observation.展开更多
Bone adhesive is a promising material for the treatment of bone fractures,which is helpful for the fast and effective reduction and fixation of broken bones.However,the existing adhesives bond weakly to bone tissues,a...Bone adhesive is a promising material for the treatment of bone fractures,which is helpful for the fast and effective reduction and fixation of broken bones.However,the existing adhesives bond weakly to bone tissues,and are non-absorbable,or hard to cure under wet conditions.Herein,inspired by the cement-based adhesive used in the industry field,we report a bioactive calcium and magnesium phosphate bone adhesive(MPBA)with the properties of facile preparation,robust adhesion,and bioactive.MPBA is equipped with similar strength to cancellous bones and shows reliable bonding performance for various interfaces,such as Ti6Al4V,Al2O3,and poly(ether-ether-ketone).MPBA achieves excellent bonding ability for the above interfaces with the bonding strengths of 2.28±0.47,2.32±0.15,and 1.44±0.38 MPa,respectively.Besides,it also shows reliable fixation ability for bovine bone surfaces.The bonding behavior to materials and bones suggests that MPBA could be used for both fracture treatment and implant fixation.Meanwhile,MPBA possesses good biological activity,which could promote the vascularization process and osteogenic differentiation.Finally,in vivo experiments confirmed MPBA can effectively restore bone strength and promote bone regeneration.展开更多
基金This work was founded by the“AO Trauma Deutschland Nachwuchsf¨orderung”(PK)as well as the German Research Foundation(DFGproject no.449121904)(AL,MG).
文摘Calcium phosphate cements (CPC) are currently widely used bone replacement materials with excellent bioactivity, but have considerable disadvantages like slow degradation. For critical-sized defects, however, an improved degradation is essential to match the tissue regeneration, especially in younger patients who are still growing. We demonstrate that a combination of CPC with mesoporous bioactive glass (MBG) particles led to an enhanced degradation in vitro and in a critical alveolar cleft defect in rats. Additionally, to support new bone formation the MBG was functionalized with hypoxia conditioned medium (HCM) derived from rat bone marrow stromal cells. HCM-functionalized scaffolds showed an improved cell proliferation and the highest formation of new bone volume. This highly flexible material system together with the drug delivery capacity is adaptable to patient specific needs and has great potential for clinical translation.
基金the National Key R&D Program of China(Grant No.2018YFC1106300 and 2017YFC1105000)the National Natural Science Foundation of China(Grant No.52072398,51802340,31870956,81860385,81672227,U2001221,51772210)+2 种基金the Frontier Science Key Research Programs of CAS(Grant No.QYZDB-SSW-JSC030)the Shenzhen Significant Strategy Layout Project(Grant No.JCYJ20170413162104773 and JCYJ20200109114620793)Beijing Municipal Health Commission(Grant No.BMHC-2018-4,BMHC-2019-9,PXM2020_026275_000002).
文摘Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects.
基金The authors acknowledge financial support for granting Open Access from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No.857287.
文摘With the increase of global population,people’s life expectancy is growing as well.Humans tend to live more active lifestyles and,therefore,trauma generated large defects become more common.Instances of tumour resection or pathological conditions and complex orthopaedic issues occur more frequently increasing necessity for bone substitutes.Composition of calcium phosphate cements(CPCs)is comparable to the chemical structure of bone minerals.Their ability to self-set and resorb in vivo secures a variety of potential applications in bone regeneration.Despite the years-long research and several products already reaching the market,finding the right properties for calcium phosphate cement to be osteoinductive and both injectable and suitable for clinical use is still a sudoku.This article is focused on injectable,porous CPCs,reviewing the latest developments on the path toward finding osteoinductive material,which is suitable for injection.
基金the National Natural Science Foundation of China(Dan Meng,81600901).
文摘Calcium phosphate cements(CPCs)have been widely used as bone graft substitutes for many years.The aim of this study was to evaluate the biocompatibility of two novel injectable,bioactive cements:b-tricalcium phosphate(b-TCP)/CPC and chitosan microsphere/CPC in vitro and in vivo.This was accomplished by culturing mouse pre-osteoblastic cells(MC3T3-E1)on discs and pastes of CPCs.Cell growth,adhesion,proliferation and differentiation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and alkaline phosphatase assays as well as by scanning electron microscopy and fluorescence.The effect of CPC paste curing was also evaluated.Implantation of two materials into the muscle tissue of rabbits was also studied and evaluated by histological analysis.Cell analysis indicated good biocompatibility in vitro.The fluorescence assay suggested that the cured material discs had no obvious effect on cell growth,while the curing process did.Histological examination showed no inflammatory cell infiltration into soft tissue.These data suggest that b-TCP/CPC and chitosan microsphere/CPC composites may be promising injectable material for bone tissue engineering.
基金financially support by Russian Foundation for Basic Research(Grant No.18-33-20170).
文摘Calcium phosphate cements are of great interest for researchers and their applications in medical practice expanded.Nevertheless,they have a number of drawbacks including the insufficient level of mechanical properties and low degradation rate.Struvite(MgNH4PO4)-based cements,which grew in popularity in recent years,despite their neutral pH and acceptable mechanical performance,release undesirable NH4+ions during their resorption.This issue could be avoided by replacement of ammonia ions in the cement liquid with sodium,however,such cements have a pH values of 9–10,leading to cytotoxicity.Thus,the main goal of this investigation is to optimize the composition of cements to achieve the combination of desirable properties:neutral pH,sufficient mechanical properties,and the absence of cytotoxicity,applying Na2HPO4-based cement liquid.For this purpose,cement powders precursors in the CaO-MgO-P2O5 system were synthesized by one-pot process in a wide composition range,and their properties were investigated.The optimal performance was observed for the cements with(Ca+Mg)/P ratio of 1.67,which are characterized by newberyite phase formation during setting reaction,pH values close to 7,sufficient compressive strength up to 22±3 MPa(for 20 mol.%of Mg),dense microstructure and adequate matrix properties of the surface.This set of features make those materials promising candidates for medical applications.
基金National Natural Science Foundation of China(Grant No.52035012)Fundamental Research Funds for the Central Universities(2682020ZT91)+1 种基金Basic Research Foundation Key Project of Sichuan Province(2021JY0046)Basic Research Foundation of Sichuan Province(2022JDRC0088).
文摘The utilization of Calcium Phosphate Cement(CPC)is limited due to its low mechanical strength and difficulty to seed cells deep into the scaffold.The objectives of this study were to:(1)develop a 3D-printed CPC-dopamine-metformin scaffold encapsulating human periodontal ligament stem cells(hPDLSCs),(2)investigate the effect of dopamine on the performance of CPC,and(3)evaluate the effect of microbead degradation and metformin release on the osteogenic differentiation of the released hPDLSCs.The mechanical property of the CPC scaffolds was elevated by adding dopamine,and the CPC scaffold with 7 wt.%dopamine had the highest compressive strength(7.35 MPa).Four types of microbeads with different content of alginate(oxidized alginate),hPDLSCs,and 2%metformin were fabricated.Morphological and cell counting kit tests confirm that the hPDLSCs are protected by microbeads encapsulation during the CPC setting process.The alkaline phosphatase test indicates that the osteogenic differentiation of hPDLSCs was enhanced by the fast release of cells and metformin.The microbeads consisting of 2%oxidized alginate and 2%metformin were optimal for cell delivery due to favorable cell release and osteogenic differentiation.This CPC scaffold is promising used for bone regeneration in dental,craniofacial,and orthopedic applications.
文摘Thanks to their biocompatibility,biodegradability,injectability and self-setting properties,calcium phosphate cements(CPCs)have been the most economical and effective biomaterials of choice for use as bone void fillers.They have also been extensively used as drug delivery carriers owing to their ability to provide for a steady release of various organic molecules aiding the regeneration of defective bone,including primarily antibiotics and growth factors.This review provides a systematic compilation of studies that reported on the controlled release of drugs from CPCs in the last 25 years.The chemical,compositional and microstructural characteristics of these systems through which the control of the release rates and mechanisms could be achieved have been discussed.In doing so,the effects of(i)the chemistry of the matrix,(ii)porosity,(iii)additives,(iv)drug types,(v)drug concentrations,(vi)drug loading methods and(vii)release media have been distinguished and discussed individually.Kinetic specificities of in vivo release of drugs from CPCs have been reviewed,too.Understanding the kinetic and mechanistic correlations between the CPC properties and the drug release is a prerequisite for the design of bone void fillers with drug release profiles precisely tailored to the application area and the clinical picture.The goal of this review has been to shed light on these fundamental correlations.
基金financial support from the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 857287(BBCE).
文摘Out of the wide range of calcium phosphate(CaP)biomaterials,calcium phosphate bone cements(CPCs)have attracted increased attention since their discovery in the 1980s due to their valuable properties such as bioactivity,osteoconductivity,injectability,hardening ability through a low-temperature setting reaction and moldability.Thereafter numerous researches have been performed to enhance the properties of CPCs.Nonetheless,low mechanical performance of CPCs limits their clinical application in load bearing regions of bone.Also,the in vivo resorption and replacement of CPC with new bone tissue is still controversial,thus further improvements of high clinical importance are required.Bioactive glasses(BGs)are biocompatible and able to bond to bone,stimulating new bone growth while dissolving over time.In the last decades extensive research has been performed analyzing the role of BGs in combination with different CaPs.Thus,the focal point of this review paper is to summarize the available research data on how injectable CPC properties could be improved or affected by the addition of BG as a secondary powder phase.It was found that despite the variances of setting time and compressive strength results,desirable injectable properties of bone cements can be achieved by the inclusion of BGs into CPCs.The published data also revealed that the degradation rate of CPCs is significantly improved by BG addition.Moreover,the presence of BG in CPCs improves the in vitro osteogenic differentiation and cell response as well as the tissue-material interaction in vivo.
基金National Key R&D Program of China(2018YFE0201500)National Natural Science Foundation of China(81772317,82272457 and 51973060)+4 种基金National Natural Science Foundation of China for Innovative Research Groups(51621002)“Technology Innovation Action Plan”of Shanghai Science and Technology Commission(21S11902700)Natural Science Foundation of Shanghai(21ZR1412300)Shanghai Talent Development Fund(2020067)Shanghai“Rising Stars of Medical Talent”Youth Development Program(Youth Medical Talents–Specialist Program,[2020]087).
文摘Injectable materials show their special merits in regeneration of damaged/degenerated bones in minimally-invasive approach.Injectable calcium phosphate bone cement(CPC)has attracted broad attention for its bioactivity,as compared to non-degradable polymethyl methacrylate cement.However,its brittleness,poor anti-washout property and uncontrollable biodegradability are the main challenges to limit its further clinical application mainly because of its stone-like dense structure and fragile inorganic-salt weakness.Herein,we developed a kind of injectable CPC bone cement with porous structure and improved robustness by incorporating poly(lactide-co-glycolic acid)(PLGA)nanofiber into CPC,with carboxymethyl cellulose(CMC)to offer good injectability as well as anti-wash-out capacity.Furthermore,the introduction of PLGA and CMC also enabled a formation of initial porous structure in the cements,where PLGA nanofiber endowed the cement with a dynamically controllable biodegradability which provided room for cell movement and bone ingrowth.Inter-estingly,the reinforced biodegradable cement afforded a sustainable provision of Ca^(2+)bioactive components,together with its porous structure,to improve synergistically new bone formation and osteo-integration in vivo by using a rat model of femur condyle defect.Further study on regenerative mechanisms indicated that the good minimally-invasive bone regeneration may come from the synergistic enhanced osteogenic effect of calcium ion enrichment and the improved revascularization capacity contributed from the porosity as well as the lactic acid released from PLGA nanofiber.These results indicate the injectable bone cement with high strength,anti-washout property and controllable biodegradability is a promising candidate for bone regeneration in a minimally-invasive approach.
基金the Natural Science Foundation of Science and Technology Commission of Shanghai Municipality (Nos.09JC1411700 and S30206)the Natural Science Foundation of Shanghai Jiaotong University School of Medicine(No.09XJ21030)
文摘The aim of the study was to analyze the histologic and ultrastructural changes after maxillary sinus augmentation with simultaneous implant placement using engineered bone graft material.In this study,calcium phosphate cement(CPC)scaffolds combined with goat bone marrow stromal cells(BMSCs)were used to fill goat sinus floor space after maxillary sinus floor elevation with simultaneous implant placement comparing with those not filled any grafted materials and used as controls.After a healing period of 3 months,the goat maxillary sinus membrane was examined using light microscopy and scanning electronic microscopy.The results showed that the connective tissue thickness and the epithelium thickness of mucosa were not statistically significant difference between two groups.The tissue engineered bone complex might be an ideal graft for the sinus floor elevation and have no influence on the sinus membrane under the histological and ultrastructural observation.
基金supported by grants from the National Key Research and Development Program of China(No.2022YFB4601402)the National Natural Science Foundation of China(Nos.32201109,51772233,and 51861145306)+2 种基金the Key Basic Research Program of Shenzhen(No.JCYJ20200109150218836)the Guangdong Basic and Applied Basic Research Foundation(Nos.2022B1515120052 and 2021A1515110557)the Laboratory Self-innovation Research Funding Project of Hanjiang Laboratory(No.HJL202202A002).
文摘Bone adhesive is a promising material for the treatment of bone fractures,which is helpful for the fast and effective reduction and fixation of broken bones.However,the existing adhesives bond weakly to bone tissues,and are non-absorbable,or hard to cure under wet conditions.Herein,inspired by the cement-based adhesive used in the industry field,we report a bioactive calcium and magnesium phosphate bone adhesive(MPBA)with the properties of facile preparation,robust adhesion,and bioactive.MPBA is equipped with similar strength to cancellous bones and shows reliable bonding performance for various interfaces,such as Ti6Al4V,Al2O3,and poly(ether-ether-ketone).MPBA achieves excellent bonding ability for the above interfaces with the bonding strengths of 2.28±0.47,2.32±0.15,and 1.44±0.38 MPa,respectively.Besides,it also shows reliable fixation ability for bovine bone surfaces.The bonding behavior to materials and bones suggests that MPBA could be used for both fracture treatment and implant fixation.Meanwhile,MPBA possesses good biological activity,which could promote the vascularization process and osteogenic differentiation.Finally,in vivo experiments confirmed MPBA can effectively restore bone strength and promote bone regeneration.
