Cancer stem cells(CSCs)are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure,tumor recurrence and metastasis.Today,the ability of many ph...Cancer stem cells(CSCs)are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure,tumor recurrence and metastasis.Today,the ability of many phytochemicals in targeting of CSCs has been identified.Kaempferol is a plant phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction,cell cycle arrest and inhibition of angiogenesis,migration,and invasion of cancer cells.In this study,we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol on MCF-7 breast CSCs.Results showed that both docetaxel and kaempferol caused a dose-dependent reduction in cell proliferation.Rhodamin 123 dye staining and flow cytometry analysis revealed that after docetaxel and kaempferol treatment,SP cells proportion of MCF-7 decreased to 11.83±2.2% and 3±0.25% respectively relative to proportion of untreated control cells(23±3.2%).Moreover,real time PCR results showed that kaempferol treatment of MCF-7 was more effective than docetaxel in down-regulating the CSC-associated markers(i.e.oct4,nanog,abcb1 and aldh1a1).Taken together,these results indicate that kaempferol is an effective anti-CSCs agent,therefore it may be a therapeutic strategy for eradicating breast cancer through the elimination of CSCs.展开更多
基金supported by a grant from Kerman University of Medical Science,Kerman,Iran(grant number:96000144).
文摘Cancer stem cells(CSCs)are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure,tumor recurrence and metastasis.Today,the ability of many phytochemicals in targeting of CSCs has been identified.Kaempferol is a plant phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction,cell cycle arrest and inhibition of angiogenesis,migration,and invasion of cancer cells.In this study,we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol on MCF-7 breast CSCs.Results showed that both docetaxel and kaempferol caused a dose-dependent reduction in cell proliferation.Rhodamin 123 dye staining and flow cytometry analysis revealed that after docetaxel and kaempferol treatment,SP cells proportion of MCF-7 decreased to 11.83±2.2% and 3±0.25% respectively relative to proportion of untreated control cells(23±3.2%).Moreover,real time PCR results showed that kaempferol treatment of MCF-7 was more effective than docetaxel in down-regulating the CSC-associated markers(i.e.oct4,nanog,abcb1 and aldh1a1).Taken together,these results indicate that kaempferol is an effective anti-CSCs agent,therefore it may be a therapeutic strategy for eradicating breast cancer through the elimination of CSCs.
