Intestinal microbes are closely related to vital host functions such as digestion and nutrient absorption,which play important roles in enhancing host adaptability.As a natural“laboratory”,caves provide an outstandi...Intestinal microbes are closely related to vital host functions such as digestion and nutrient absorption,which play important roles in enhancing host adaptability.As a natural“laboratory”,caves provide an outstanding model for understanding the significance of gut microbes and feeding habits in the habitat adaptability of hosts.However,research on the relationship between gut microbes,feeding habits,and the adaptability of troglobites remains insufficient.In this study,we compared the characteristics of the intestinal microbes of Sinocyclocheilus cavefish and surface fish and further established the relationship between intestinal and habitat microbes.Furthermore,we conducted environmental DNA(eDNA)(metabarcoding)analysis of environmental samples to clarify the composition of potential food resources in the habitats of the Sinocyclocheilus cavefish and surface fish.Results showed that the structure of the Sinocyclocheilus gut microbes was more related to ecological type(habitat type)than phylogenetic relationships.While horizontal transfer of habitat microbes was a source of gut microbes,hosts also showed strong selection for inherent microbes as dominant microorganisms.Differences in the composition and structure of gut microbes,especially dominant microbes,may enhance the adaptability of the two Sinocyclocheilus fish types from the perspectives of food intake,nutrient utilization,and harmful substance metabolism,suggesting that food resources,predation patterns,intestinal flora,digestive and absorptive capacity,and feeding habits and preferences are linked to habitat adaptability.These results should facilitate our understanding of the significance of fish gut microbes to habitat adaptation and provide a new perspective for studying the adaptive mechanisms of cavefish.展开更多
The vagina has diverse vaginal microbes(Vm).A disturbance in the delicate balance maintained in Vm is linked to women's obstetrical and reproductive tract problems.Vaginal microbes play an essential role in protec...The vagina has diverse vaginal microbes(Vm).A disturbance in the delicate balance maintained in Vm is linked to women's obstetrical and reproductive tract problems.Vaginal microbes play an essential role in protecting the health of the female reproductive tract by alleviating gynecological infection.However,Vm profiling has many confounders that need to be addressed during sampling,including age,race,pregnancy,medical illness,and smoking.Vm profiling improves reproduction odds,may serve as a marker for genital malignancies and have a therapeutic application in menopausal women and women with cervical cancers.展开更多
Background Anthocyanins(AC)showed positive effects on improving the intestinal health and alleviating intestinal pathogen infections,therefore,an experiment was conducted to explore the protective effects of supplemen...Background Anthocyanins(AC)showed positive effects on improving the intestinal health and alleviating intestinal pathogen infections,therefore,an experiment was conducted to explore the protective effects of supplemented AC on Salmonella-infected chickens.Methods A total of 240 hatchling chickens were randomly allocated to 4 treatments,each with 6 replicates.Birds were fed a basal diet supplemented with 0(CON,and ST),100(ACL)and 400(ACH)mg/kg of AC for d 60,and orally challenged with PBS(CON)or 10^(9) CFU/bird(ST,ACL,ACH)Salmonella Typhimurium at d 14 and 16.Results(1)Compared with birds in ST,AC supplementation increased the body weight(BW)at d 18 and the average daily gain(ADG)from d 1 to 18 of the Salmonella-infected chickens(P<0.05);(2)AC decreased the number of Salmonella cells in the liver and spleen,the contents of NO in plasma and inflammatory cytokines in ileal mucosa of Salmonella-infected chickens(P<0.05);(3)Salmonella infection decreased the ileal villi height,villi height to crypt depth(V/C),and the expression of zonulaoccludins-1(ZO-1),claudin-1,occludin,and mucin 2(MUC2)in ileal mucosa.AC supplementation relieved these adverse effects,and decreased ileal crypt depth(P<0.05);(4)In cecal microbiota of Salmonella-infected chickens,AC increased(P<0.05)the alpha-diversity(Chao1,Pd,Shannon and Sobs indexes)and the relative abundance of Firmicutes,and decreased(P<0.05)the relative abundance of Proteobacteria and Bacteroidota and the enrichment of drug antimicrobial resistance,infectious bacterial disease,and immune disease pathways.Conclusions Dietary AC protected chicken against Salmonella infection via inhibiting the Salmonella colonization in liver and spleen,suppressing secretion of inflammatory cytokines,up-regulating the expression of ileal barrier-related genes,and ameliorating the composition and function of cecal microbes.Under conditions here used,100 mg/kg bilberry anthocyanin was recommended.展开更多
High blood pressure(BP),known as hypertension,is a major contributing factor to the development of cardiovascular disease.The development and pathogenesis of hypertension involve a wide array of factors including gene...High blood pressure(BP),known as hypertension,is a major contributing factor to the development of cardiovascular disease.The development and pathogenesis of hypertension involve a wide array of factors including genetics,environment,hormones,hemodynamics,and inflammation.There is a significantly positive association between higher levels of colonization by Aggregatibacter actinomycetemcomitans,Porphyromonas gingivalis,Tannerella forsythia,and Treponema denticola(etiologic bacterial burden)below the gum line,and the presence of hypertension.The use of antibiotics during pregnancy,which is likely indicative of bacterial infections severe enough to require antibiotic treatment,is associated with a slight increase in average arterial BP.Cytomegalovirus infection is a risk factor for heightened arterial BP and acts as a co-factor in the development of aortic atherosclerosis.The relationship between hypertension and coronavirus disease 2019 involves endothelial dysfunction and dysregulation of the renin-angiotensin system.The effects of gut microbiota on BP,whether beneficial or harmful,are influenced by multiple factors including genetics,epigenetics,lifestyle choices,and antibiotic usage.These variables collectively contribute to overall BP levels and the control of hypertension.Several reports have examined the BP levels of patients infected with the Zika virus.In regions with a high incidence of nasopharyngeal carcinoma,hypertension has been linked to a higher risk of Epstein-Barr virus reactivation.Also,a potential causal link has been found between malaria and elevated BP.Also,the elevated prevalence of hypertension among dengue patients during their initial visit suggests that relying solely on BP measurements to predict severe infection may not be clinically reliable.展开更多
In the Loess Plateau of China,land-use pattern is a major factor in controlling underlying biological processes.Additionally,the process of land-use pattern was accompanied by abandoned lands,potentially impacting soi...In the Loess Plateau of China,land-use pattern is a major factor in controlling underlying biological processes.Additionally,the process of land-use pattern was accompanied by abandoned lands,potentially impacting soil microbe.However,limited researches were conducted to study the impacts of land-use patterns on the diversity and community of soil microorganisms in this area.The study aimed to investigate soil microbial community diversity and composition using high-throughput deoxyribonucleic acid(DNA)sequencing under different land-use patterns(apricot tree land,apple tree land,peach tree land,corn land,and abandoned land).The results showed a substantial difference(P<0.050)in bacterial alpha-diversity and beta-diversity between abandoned land and other land-use patterns,with the exception of Shannon index.While fungal beta-diversity was not considerably impacted by land-use patterns,fungal alpha-diversity indices varied significantly.The relative abundance of Actinobacteriota(34.90%),Proteobacteria(20.65%),and Ascomycota(77.42%)varied in soils with different land-use patterns.Soil pH exerted a dominant impact on the soil bacterial communities'composition,whereas soil available phosphorus was the main factor shaping the soil fungal communities'composition.These findings suggest that variations in land-use pattern had resulted in changes to soil properties,subsequently impacting diversity and structure of microbial community in the Loess Plateau.Given the strong interdependence between soil and its microbiota,it is imperative to reclaim abandoned lands to maintain soil fertility and sustain its function,which will have significant ecological service implications,particularly with regards to soil conservation in ecologically vulnerable areas.展开更多
Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for trea...Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for treatment of textile wastewater containing dyes and PVA. Activated carbon adsorption was used as a tertiary treatment stage, and residual sludge from clarifier returned to the anaerobic reactor again. The pilot test were carried out with two systems. One was inoculated by acclimated sludge, and the another was adding the mixed culture of dye-decoloring and PVA-degrading bacteria for forming biological films, the latter was observed to be more effective than the former. The test has run normally for ten months with a COD loading of 2.13 kg/m3/day, a BOD5 loading of 0.34 kg/m3/day in anaerobic reactor; a COD loading of 1.71 kg/m3/day, a BOD5 loading 0.44 kg/m3/day in aerobic reactor. The pollutants removal efficiency by adding microbes was about 20% higher than that by acclimated sludge. The展开更多
The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stabi...The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.展开更多
Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0 2～12 m and being more ple...Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0 2～12 m and being more plentiful within 3 m in the profile,include bacteria (Bacillus,Enterobacter, Escherichia, Alkaligenes, Neisseria, Staphylococcus and anaerobic bacteria such as Clostridium), fungi (Aspergillus niger, Aspergillus flavus, Penicillium, Mucor and Saccharomycete) and actinomyces. Experiments were made under room temperature by using solutions of the cultured microbes and their metabolites, compared with distilled water and the culture solution without microbes, to leach RE from the sample of the weathering crust. The results are shown by the experiments: (1) The mixed microbes cultured from the profile and their metabolites increase the quantity of RE leached from the sample and reduce the pH of the solutions. (2) The ability to leach RE from the sample varies with various microbes, decreasing in a sequence of fungi (Mucor, saccharomycete, Aspegillus and Penicillium), zymotic bacilli (Enterobacter, Escherichia etc.), Staphylococcus, zymotic Bacillus, actinomyces and Alkaligenes. (3) The RE leached with bacteria is mainly related to the pH value of the solutions influenced by the metabolites of the bacteria; whereas that leached with fungi is mainly related to the adsorption and imbibition of RE by the fungi and the complexing of RE with their metabolites. (4) Compared with that leached with ammonium sulfate, the fractionation of the RE leached with microbes is characterized by higher δ Ce , lower δ Eu and lower ratios of N La/Sm and N Gd/Yb . The result of the fractionation of RE accords with the distribution of RE in the various layers of a profile of weathering crust of granite in South China. The experimental results indicate that microbes and their metabolites should play a positive role in the mobilization, migration and fractionation of RE in the weathering crust in South China.展开更多
Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydro...Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydrocarbon Meter (GHM), by experimental analysis qualitatively and quantitatively in the paper. Analytical result showed that the crude oil could be considerably degraded by eating-oil microbes in oily soil and the number of eating-oil microbes increased while the working hours of oil-well risi...展开更多
Diversity analysis on secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds, has been carried out for under...Diversity analysis on secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds, has been carried out for understanding of their chemical constituents. The methanol extracts of Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011 were subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites, respectively. The chemical constituents of NJ-011 are mainly N-containing compounds including some alkaloids and short polypeptides, while those of NJ-008 are not N-containing ones. Three compounds were also isolated and identified from extract of NJ011 by different column chromatography and preparative HPLC, and their structures were elucidated as b-carboline (1), 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (3) by comparison of TOF MS, 1Hand 13C-NMR data with those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with complicated structures.展开更多
BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-tration o...BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-tration of metal ions (0.2 mM to 1.4 mM), pH (3 to 8) and temperature of incubation (15℃, 20℃, 25℃, 30℃ and 35℃). Results of BOD inhibition are supported by absorbance measurement (OD600) studies of microbial matter preserved in Luriya broth medium. OD measurement results are used to derive minimum inhibitory concentration, i.e., threshold concentration of each metal showing toxicity towards microbes. Sil-ver is found to be the most toxic element.展开更多
Laboratory and in situ experiments were done to investigate the influences of cultivation on temperate semi arid grassland (for 17 years spring wheat planted once every two years without fertilization) on soil N 2O em...Laboratory and in situ experiments were done to investigate the influences of cultivation on temperate semi arid grassland (for 17 years spring wheat planted once every two years without fertilization) on soil N 2O emission and quantitative variations of related soil microbes. In the laboratory (25℃ and soil moisture 18%), cultivation increased soil transformations of fertilizer nitrogen (100 μg N/g as NaNO 3, urea, or as urea with dicyandiamide 1 μg N/g). The N 2O emissions from the cultivated and uncultivated soils with or without nitrogen additions were relatively low, and mainly originated from the nitrification. The soil N 2O emission due to cultivation decreased somewhat upon no fertilization or NaNO 3 addition, but significantly upon urea addition. The role of dicyandiamide as nitrification inhibitor was only considerable in the cultivated soil, and had small influence on decreasing N 2O emission in the two soils. The influence of cultivation on soil N 2O emission was also reflected by the number variations of microbes related with soil nitrogen transformation in the two soils. Compared to the uncultivated grassland, in situ ammonifiers and denitrifiers in the cultivated grassland quantitatively averagely increased, and aerobic no symbiotic azotobacters were quantitatively similar, leading to the continued decrease of organic matter content and the decrease of N 2O emission from the cultivated grassland soil.展开更多
The action of etching and damage by 20 keV N+ beam on the cells of Deinococcus radiodurans and Escherichia coli was investigated by scanning electron microscope (SEM) and the electron spin resonance (ESR) spectrum of ...The action of etching and damage by 20 keV N+ beam on the cells of Deinococcus radiodurans and Escherichia coli was investigated by scanning electron microscope (SEM) and the electron spin resonance (ESR) spectrum of free radicals. The results showed that N+ implantation exerted the direct action of etching and damage of momentum transferring and the indirect action of the free radicals of energy deposition on their cells, many microholes were found on the surface of cells’ wall and /or membrane by SEM, the damaged DNA was determined using DNA unwinding technique, and the signal of free radicals was measured by ESR. The degree of damage to cells by ion beam gradually increased with the increase implantation dose. With the post-treatment of 2 mmol/l caffeine and 0.5 mmol / l Na2-EDTA, the survival rate of D.radiodurans and E.coli further decreased in the order of caffeine > Na2-EDTA > control, and this suggested that low energy ion beam could be implanted into nucleus, doing a damage to DNA and resulting in the mutation of organisms.展开更多
Hypertension is an important global public health issue because of its high morbidity as well as the increased risk of other diseases.Recent studies have indicated that the development of hypertension is related to th...Hypertension is an important global public health issue because of its high morbidity as well as the increased risk of other diseases.Recent studies have indicated that the development of hypertension is related to the dysbiosis of the gut microbiota in both animals and humans.In this review,we outline the interaction between gut microbiota and hypertension,including gut microbial changes in hypertension,the effect of microbial dysbiosis on blood pressure(BP),indicators of gut microbial dysbiosis in hypertension,and the microbial genera that affect BP at the taxonomic level.For example,increases in Lactobacillus,Roseburia,Coprococcus,Akkermansia,and Bifidobacterium are associated with reduced BP,while increases in Streptococcus,Blautia,and Prevotella are associated with elevated BP.Furthermore,we describe the potential mechanisms involved in the regulation between gut microbiota and hypertension.Finally,we summarize the commonly used treatments of hypertension that are based on gut microbes,including fecal microbiota transfer,probiotics and prebiotics,antibiotics,and dietary supplements.This review aims to find novel potential genera for improving hypertension and give a direction for future studies on gut microbiota in hypertension.展开更多
Microbes were cultured and identified from the samples collected at various depths in 4 weathering profiles of REE\|bearing granites in Gonghe, Guangdong. The microbes were found existing at the depth of 0-5 m in all ...Microbes were cultured and identified from the samples collected at various depths in 4 weathering profiles of REE\|bearing granites in Gonghe, Guangdong. The microbes were found existing at the depth of 0-5 m in all the profiles. The main microbes include coccus (Staphylococcus, Streptococcus), bacillus (Bacillus, Clostridium and Escherichia Coli), actinomyces and fungi (Saccharomycete, Penicillium, Fusarium, Aspergillus Aiger and Mucor). The number of colonies decreases downwards in the profiles. Experimental studies show that all the microbes used in the experiment can accelerate downward migration of REE in the experimental tubes. The ability to accelerate the migration of REE decreases in a sequence of fungi→actinomyces→bacillus→coccus. The microbes can change the modes of occurrence of REE in the weathering crust. The coccus, bacillus and actinomyces can increase the amounts of REE in ion state, whereas the fungi have a stronger ability to form organic compounding REE and accumulate REE than the bacteria do.展开更多
The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was studied ...The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was studied by means of Calvet microcalorimeter. The rate constant k of microbe growth was calculated, and the inhibition effect of these complexes to microbes was compared.展开更多
Studies were conducted to identify candidate soil microbes responsible for observed differences in strawberry vigour at a small spatial scale, which was not associated with visual disease symptoms. Samples were obtain...Studies were conducted to identify candidate soil microbes responsible for observed differences in strawberry vigour at a small spatial scale, which was not associated with visual disease symptoms. Samples were obtained from the soils close to the rhizosphere of ‘big' and ‘small' plants from small plots which exhibited large local heterogeneity in plant vigour. A metabarcoding approach was used to profile bacterial and fungal compositions, using two primer pairs for 16 S ribosomal RNA genes(16S r DNA) and one for the fungal internal transcribed spacer(ITS) region. Of the two 16 S r DNA primer sets, the 341F/805 R resulted in sequences of better quality. A total 28 operational taxonomic units(OTUs) had differential relative abundance between samples from ‘big' and ‘small' plants. However, plausible biological explanation was only possible for three fungal OTUs. Two were possible phytopathogens: Verticillium spp. and Alternaria alternata although the latter has never been considered as a main pathogen of strawberry in the UK. For samples from ‘small' plants, the abundance of these OTUs was much greater than from ‘big' plants. The opposite was true for a mycorrhizal OTU. These results suggest that soil microbes related to crop production can be identified using metabarcoding technique. Further research is needed to assess whether A. alternata and Verticillium spp. could affect strawberry growth in the field.展开更多
Bruguiera sexangula(Lour) Poir., a threatened mangrove tree, was inoculated with beneficial microbes in a nursery to assess any improvements in growth and biomass.From soil samples from the rhizosphere of B.sexangula ...Bruguiera sexangula(Lour) Poir., a threatened mangrove tree, was inoculated with beneficial microbes in a nursery to assess any improvements in growth and biomass.From soil samples from the rhizosphere of B.sexangula in a mangrove forest in Panangadu of Kerala India, nitrogen-fixing bacteria Azotobacter chroococcum and Azospirillum brasilense were isolated.The phosphatesolubilising bacterium Bacillus megaterium and potassiummobilizing bacteria Frateruria aurantia were also isolated and cultured on suitable media.Later, ripe propagules of B.sexangula were collected from matured trees and raised in sterilized soil bags(13×25 cm) containing sterilized soil and sand(2:1 ratio).The cultured beneficial microbes were propagated and used to inoculate the ripe propagules of B.sexangula and maintained in the nursery for 6 months.After 6 months, growth and biomass of the inoculated propagules were greater than for the uninoculated control propagules.Shoot length, number of leaves, stem girth and root length were also significantly greater than in the controls.This study showed that the mangrove-specific beneficial microbes influenced the growth of B.sexangula in the nursery and will help in the establishment of B.sexangula in degraded mangrove forests.展开更多
It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%,and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%,lower than that of DZ...It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%,and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%,lower than that of DZW-47.The inhibitory mycelia growth mechanism of different strains on R.solani was quite different,with DZW-3 mainly on the aspect of hyperparasitism, DZW-21 on the synergism of hyperparasitism and metabolite,DZW-47 on the synergism of nutrient competition and secondary metabolite,ZLR-2 and ZLR- 11 on producing secondary metabolite.Controlling efficiency of seedling bed accorded basically with that of the broth.The controlling efficiency of DZW-47,ZLR-2,ZLR-11,DZW-21 and DZW-3 were 97.20%,95.7%,94.6%,93.6% and 89.20%,respectively.展开更多
基金supported by the National Natural Science Foundation of China(32260310,31560111)Top Young Talents Program of the Ten-Thousand Plan of Yunnan Province(YNWR-QNBJ-2018-024)Scientific Research Fund of Yunnan Provincial Education Department,China(2020Y0014)。
文摘Intestinal microbes are closely related to vital host functions such as digestion and nutrient absorption,which play important roles in enhancing host adaptability.As a natural“laboratory”,caves provide an outstanding model for understanding the significance of gut microbes and feeding habits in the habitat adaptability of hosts.However,research on the relationship between gut microbes,feeding habits,and the adaptability of troglobites remains insufficient.In this study,we compared the characteristics of the intestinal microbes of Sinocyclocheilus cavefish and surface fish and further established the relationship between intestinal and habitat microbes.Furthermore,we conducted environmental DNA(eDNA)(metabarcoding)analysis of environmental samples to clarify the composition of potential food resources in the habitats of the Sinocyclocheilus cavefish and surface fish.Results showed that the structure of the Sinocyclocheilus gut microbes was more related to ecological type(habitat type)than phylogenetic relationships.While horizontal transfer of habitat microbes was a source of gut microbes,hosts also showed strong selection for inherent microbes as dominant microorganisms.Differences in the composition and structure of gut microbes,especially dominant microbes,may enhance the adaptability of the two Sinocyclocheilus fish types from the perspectives of food intake,nutrient utilization,and harmful substance metabolism,suggesting that food resources,predation patterns,intestinal flora,digestive and absorptive capacity,and feeding habits and preferences are linked to habitat adaptability.These results should facilitate our understanding of the significance of fish gut microbes to habitat adaptation and provide a new perspective for studying the adaptive mechanisms of cavefish.
文摘The vagina has diverse vaginal microbes(Vm).A disturbance in the delicate balance maintained in Vm is linked to women's obstetrical and reproductive tract problems.Vaginal microbes play an essential role in protecting the health of the female reproductive tract by alleviating gynecological infection.However,Vm profiling has many confounders that need to be addressed during sampling,including age,race,pregnancy,medical illness,and smoking.Vm profiling improves reproduction odds,may serve as a marker for genital malignancies and have a therapeutic application in menopausal women and women with cervical cancers.
基金financially supported by Natural Science Foundation from Guangdong Province (2021A1515010830,2021A1515012412)National Key R&D Project (2018YFD0500600,2021YFD300404)+3 种基金China Agriculture Research System of MOF and MARA (CARS-41)the Key Realm R&D Program of Guangdong Province (2020B0202090004)National Natural Science Foundation of China (31802104)the Science and Technology Program of Guangdong Academy of Agricultural Sciences (202106TD,R2019PY-QF008),P.R.China。
文摘Background Anthocyanins(AC)showed positive effects on improving the intestinal health and alleviating intestinal pathogen infections,therefore,an experiment was conducted to explore the protective effects of supplemented AC on Salmonella-infected chickens.Methods A total of 240 hatchling chickens were randomly allocated to 4 treatments,each with 6 replicates.Birds were fed a basal diet supplemented with 0(CON,and ST),100(ACL)and 400(ACH)mg/kg of AC for d 60,and orally challenged with PBS(CON)or 10^(9) CFU/bird(ST,ACL,ACH)Salmonella Typhimurium at d 14 and 16.Results(1)Compared with birds in ST,AC supplementation increased the body weight(BW)at d 18 and the average daily gain(ADG)from d 1 to 18 of the Salmonella-infected chickens(P<0.05);(2)AC decreased the number of Salmonella cells in the liver and spleen,the contents of NO in plasma and inflammatory cytokines in ileal mucosa of Salmonella-infected chickens(P<0.05);(3)Salmonella infection decreased the ileal villi height,villi height to crypt depth(V/C),and the expression of zonulaoccludins-1(ZO-1),claudin-1,occludin,and mucin 2(MUC2)in ileal mucosa.AC supplementation relieved these adverse effects,and decreased ileal crypt depth(P<0.05);(4)In cecal microbiota of Salmonella-infected chickens,AC increased(P<0.05)the alpha-diversity(Chao1,Pd,Shannon and Sobs indexes)and the relative abundance of Firmicutes,and decreased(P<0.05)the relative abundance of Proteobacteria and Bacteroidota and the enrichment of drug antimicrobial resistance,infectious bacterial disease,and immune disease pathways.Conclusions Dietary AC protected chicken against Salmonella infection via inhibiting the Salmonella colonization in liver and spleen,suppressing secretion of inflammatory cytokines,up-regulating the expression of ileal barrier-related genes,and ameliorating the composition and function of cecal microbes.Under conditions here used,100 mg/kg bilberry anthocyanin was recommended.
文摘High blood pressure(BP),known as hypertension,is a major contributing factor to the development of cardiovascular disease.The development and pathogenesis of hypertension involve a wide array of factors including genetics,environment,hormones,hemodynamics,and inflammation.There is a significantly positive association between higher levels of colonization by Aggregatibacter actinomycetemcomitans,Porphyromonas gingivalis,Tannerella forsythia,and Treponema denticola(etiologic bacterial burden)below the gum line,and the presence of hypertension.The use of antibiotics during pregnancy,which is likely indicative of bacterial infections severe enough to require antibiotic treatment,is associated with a slight increase in average arterial BP.Cytomegalovirus infection is a risk factor for heightened arterial BP and acts as a co-factor in the development of aortic atherosclerosis.The relationship between hypertension and coronavirus disease 2019 involves endothelial dysfunction and dysregulation of the renin-angiotensin system.The effects of gut microbiota on BP,whether beneficial or harmful,are influenced by multiple factors including genetics,epigenetics,lifestyle choices,and antibiotic usage.These variables collectively contribute to overall BP levels and the control of hypertension.Several reports have examined the BP levels of patients infected with the Zika virus.In regions with a high incidence of nasopharyngeal carcinoma,hypertension has been linked to a higher risk of Epstein-Barr virus reactivation.Also,a potential causal link has been found between malaria and elevated BP.Also,the elevated prevalence of hypertension among dengue patients during their initial visit suggests that relying solely on BP measurements to predict severe infection may not be clinically reliable.
基金supported by the Science and Technology Planning Project of Gansu Province,China(23ZDKA017).
文摘In the Loess Plateau of China,land-use pattern is a major factor in controlling underlying biological processes.Additionally,the process of land-use pattern was accompanied by abandoned lands,potentially impacting soil microbe.However,limited researches were conducted to study the impacts of land-use patterns on the diversity and community of soil microorganisms in this area.The study aimed to investigate soil microbial community diversity and composition using high-throughput deoxyribonucleic acid(DNA)sequencing under different land-use patterns(apricot tree land,apple tree land,peach tree land,corn land,and abandoned land).The results showed a substantial difference(P<0.050)in bacterial alpha-diversity and beta-diversity between abandoned land and other land-use patterns,with the exception of Shannon index.While fungal beta-diversity was not considerably impacted by land-use patterns,fungal alpha-diversity indices varied significantly.The relative abundance of Actinobacteriota(34.90%),Proteobacteria(20.65%),and Ascomycota(77.42%)varied in soils with different land-use patterns.Soil pH exerted a dominant impact on the soil bacterial communities'composition,whereas soil available phosphorus was the main factor shaping the soil fungal communities'composition.These findings suggest that variations in land-use pattern had resulted in changes to soil properties,subsequently impacting diversity and structure of microbial community in the Loess Plateau.Given the strong interdependence between soil and its microbiota,it is imperative to reclaim abandoned lands to maintain soil fertility and sustain its function,which will have significant ecological service implications,particularly with regards to soil conservation in ecologically vulnerable areas.
文摘Some strains and culture of bacteria which are able to decolorize dyes and degrade polyvinyl alcohol(PVA) were isolated and selected. A pilot scale facultative anaerobic-aerobic biological process was applied for treatment of textile wastewater containing dyes and PVA. Activated carbon adsorption was used as a tertiary treatment stage, and residual sludge from clarifier returned to the anaerobic reactor again. The pilot test were carried out with two systems. One was inoculated by acclimated sludge, and the another was adding the mixed culture of dye-decoloring and PVA-degrading bacteria for forming biological films, the latter was observed to be more effective than the former. The test has run normally for ten months with a COD loading of 2.13 kg/m3/day, a BOD5 loading of 0.34 kg/m3/day in anaerobic reactor; a COD loading of 1.71 kg/m3/day, a BOD5 loading 0.44 kg/m3/day in aerobic reactor. The pollutants removal efficiency by adding microbes was about 20% higher than that by acclimated sludge. The
基金supported by the National Natural Science Foundation of China (31760353 and 31560360)the National Key R&D Program of China (2017YFD0300804 and 2016YFD0300103)+2 种基金the earmarked fund for China Agriculture Research System (CARS-02-63)the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120)the Advanced Talented Scholars of Inner Mongolia Agricultural University, China (NDYB2016-15)
文摘The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.
文摘Microbes were cultured from the samples at various depths in a weathering profile of RE-bearing granite in Gonghe RE mine, Guangdong Province. The cultured microbes, existing at a depth of 0 2～12 m and being more plentiful within 3 m in the profile,include bacteria (Bacillus,Enterobacter, Escherichia, Alkaligenes, Neisseria, Staphylococcus and anaerobic bacteria such as Clostridium), fungi (Aspergillus niger, Aspergillus flavus, Penicillium, Mucor and Saccharomycete) and actinomyces. Experiments were made under room temperature by using solutions of the cultured microbes and their metabolites, compared with distilled water and the culture solution without microbes, to leach RE from the sample of the weathering crust. The results are shown by the experiments: (1) The mixed microbes cultured from the profile and their metabolites increase the quantity of RE leached from the sample and reduce the pH of the solutions. (2) The ability to leach RE from the sample varies with various microbes, decreasing in a sequence of fungi (Mucor, saccharomycete, Aspegillus and Penicillium), zymotic bacilli (Enterobacter, Escherichia etc.), Staphylococcus, zymotic Bacillus, actinomyces and Alkaligenes. (3) The RE leached with bacteria is mainly related to the pH value of the solutions influenced by the metabolites of the bacteria; whereas that leached with fungi is mainly related to the adsorption and imbibition of RE by the fungi and the complexing of RE with their metabolites. (4) Compared with that leached with ammonium sulfate, the fractionation of the RE leached with microbes is characterized by higher δ Ce , lower δ Eu and lower ratios of N La/Sm and N Gd/Yb . The result of the fractionation of RE accords with the distribution of RE in the various layers of a profile of weathering crust of granite in South China. The experimental results indicate that microbes and their metabolites should play a positive role in the mobilization, migration and fractionation of RE in the weathering crust in South China.
基金Supported by the nature scientific fund of Heilongjiang province(No.110210).
文摘Production and storage-transportation of crude oil can not only give rise to soil pollution but also destroy ecological environment. Degradation of microbes for oily soil was studied with the instnunent, Geofina Hydrocarbon Meter (GHM), by experimental analysis qualitatively and quantitatively in the paper. Analytical result showed that the crude oil could be considerably degraded by eating-oil microbes in oily soil and the number of eating-oil microbes increased while the working hours of oil-well risi...
文摘Diversity analysis on secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds, has been carried out for understanding of their chemical constituents. The methanol extracts of Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011 were subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites, respectively. The chemical constituents of NJ-011 are mainly N-containing compounds including some alkaloids and short polypeptides, while those of NJ-008 are not N-containing ones. Three compounds were also isolated and identified from extract of NJ011 by different column chromatography and preparative HPLC, and their structures were elucidated as b-carboline (1), 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (3) by comparison of TOF MS, 1Hand 13C-NMR data with those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with complicated structures.
文摘BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-tration of metal ions (0.2 mM to 1.4 mM), pH (3 to 8) and temperature of incubation (15℃, 20℃, 25℃, 30℃ and 35℃). Results of BOD inhibition are supported by absorbance measurement (OD600) studies of microbial matter preserved in Luriya broth medium. OD measurement results are used to derive minimum inhibitory concentration, i.e., threshold concentration of each metal showing toxicity towards microbes. Sil-ver is found to be the most toxic element.
文摘Laboratory and in situ experiments were done to investigate the influences of cultivation on temperate semi arid grassland (for 17 years spring wheat planted once every two years without fertilization) on soil N 2O emission and quantitative variations of related soil microbes. In the laboratory (25℃ and soil moisture 18%), cultivation increased soil transformations of fertilizer nitrogen (100 μg N/g as NaNO 3, urea, or as urea with dicyandiamide 1 μg N/g). The N 2O emissions from the cultivated and uncultivated soils with or without nitrogen additions were relatively low, and mainly originated from the nitrification. The soil N 2O emission due to cultivation decreased somewhat upon no fertilization or NaNO 3 addition, but significantly upon urea addition. The role of dicyandiamide as nitrification inhibitor was only considerable in the cultivated soil, and had small influence on decreasing N 2O emission in the two soils. The influence of cultivation on soil N 2O emission was also reflected by the number variations of microbes related with soil nitrogen transformation in the two soils. Compared to the uncultivated grassland, in situ ammonifiers and denitrifiers in the cultivated grassland quantitatively averagely increased, and aerobic no symbiotic azotobacters were quantitatively similar, leading to the continued decrease of organic matter content and the decrease of N 2O emission from the cultivated grassland soil.
基金This work is supported by the National Science Foundation of china (19605005)
文摘The action of etching and damage by 20 keV N+ beam on the cells of Deinococcus radiodurans and Escherichia coli was investigated by scanning electron microscope (SEM) and the electron spin resonance (ESR) spectrum of free radicals. The results showed that N+ implantation exerted the direct action of etching and damage of momentum transferring and the indirect action of the free radicals of energy deposition on their cells, many microholes were found on the surface of cells’ wall and /or membrane by SEM, the damaged DNA was determined using DNA unwinding technique, and the signal of free radicals was measured by ESR. The degree of damage to cells by ion beam gradually increased with the increase implantation dose. With the post-treatment of 2 mmol/l caffeine and 0.5 mmol / l Na2-EDTA, the survival rate of D.radiodurans and E.coli further decreased in the order of caffeine > Na2-EDTA > control, and this suggested that low energy ion beam could be implanted into nucleus, doing a damage to DNA and resulting in the mutation of organisms.
基金Key Scientific Research Projects for Higher Education of Henan Province,Grant/Award Number:20A310012the College Student Innovation and Entrepreneurship Training Program of Henan Province,Grant/Award Number:202110472018 and S202110472046+1 种基金the Science and Technology Research Project of Henan Province,Grant/Award Number:192102310169 and 202102310270Doctoral Scientific Research Activation Foundation of the Xinxiang Medical University,Grant/Award Number:505287。
文摘Hypertension is an important global public health issue because of its high morbidity as well as the increased risk of other diseases.Recent studies have indicated that the development of hypertension is related to the dysbiosis of the gut microbiota in both animals and humans.In this review,we outline the interaction between gut microbiota and hypertension,including gut microbial changes in hypertension,the effect of microbial dysbiosis on blood pressure(BP),indicators of gut microbial dysbiosis in hypertension,and the microbial genera that affect BP at the taxonomic level.For example,increases in Lactobacillus,Roseburia,Coprococcus,Akkermansia,and Bifidobacterium are associated with reduced BP,while increases in Streptococcus,Blautia,and Prevotella are associated with elevated BP.Furthermore,we describe the potential mechanisms involved in the regulation between gut microbiota and hypertension.Finally,we summarize the commonly used treatments of hypertension that are based on gut microbes,including fecal microbiota transfer,probiotics and prebiotics,antibiotics,and dietary supplements.This review aims to find novel potential genera for improving hypertension and give a direction for future studies on gut microbiota in hypertension.
基金ThisworkwasgrantedbytheNaturalScienceFoundationofGuangdongProvince China !(GrantNo .96 0 0 42 )
文摘Microbes were cultured and identified from the samples collected at various depths in 4 weathering profiles of REE\|bearing granites in Gonghe, Guangdong. The microbes were found existing at the depth of 0-5 m in all the profiles. The main microbes include coccus (Staphylococcus, Streptococcus), bacillus (Bacillus, Clostridium and Escherichia Coli), actinomyces and fungi (Saccharomycete, Penicillium, Fusarium, Aspergillus Aiger and Mucor). The number of colonies decreases downwards in the profiles. Experimental studies show that all the microbes used in the experiment can accelerate downward migration of REE in the experimental tubes. The ability to accelerate the migration of REE decreases in a sequence of fungi→actinomyces→bacillus→coccus. The microbes can change the modes of occurrence of REE in the weathering crust. The coccus, bacillus and actinomyces can increase the amounts of REE in ion state, whereas the fungi have a stronger ability to form organic compounding REE and accumulate REE than the bacteria do.
文摘The thermochemical kinetics of the rare earth ternary complex Sm(Gly) 4Im(ClO 4) 3·2H 2O (Gly glycine, Im imidazole) reacting with microbes (escherichia coli, bacillus subilis, staphylococcus aureus) was studied by means of Calvet microcalorimeter. The rate constant k of microbe growth was calculated, and the inhibition effect of these complexes to microbes was compared.
基金funded by Innovate UK(100867)with matching funding from several commercial companiesthe financial assistance of the China Scholarship Council(201306300133 and 201506300012)
文摘Studies were conducted to identify candidate soil microbes responsible for observed differences in strawberry vigour at a small spatial scale, which was not associated with visual disease symptoms. Samples were obtained from the soils close to the rhizosphere of ‘big' and ‘small' plants from small plots which exhibited large local heterogeneity in plant vigour. A metabarcoding approach was used to profile bacterial and fungal compositions, using two primer pairs for 16 S ribosomal RNA genes(16S r DNA) and one for the fungal internal transcribed spacer(ITS) region. Of the two 16 S r DNA primer sets, the 341F/805 R resulted in sequences of better quality. A total 28 operational taxonomic units(OTUs) had differential relative abundance between samples from ‘big' and ‘small' plants. However, plausible biological explanation was only possible for three fungal OTUs. Two were possible phytopathogens: Verticillium spp. and Alternaria alternata although the latter has never been considered as a main pathogen of strawberry in the UK. For samples from ‘small' plants, the abundance of these OTUs was much greater than from ‘big' plants. The opposite was true for a mycorrhizal OTU. These results suggest that soil microbes related to crop production can be identified using metabarcoding technique. Further research is needed to assess whether A. alternata and Verticillium spp. could affect strawberry growth in the field.
基金funded by the Program of Department of Science and Technology,New Delhi,Government of India(No.IF110661)
文摘Bruguiera sexangula(Lour) Poir., a threatened mangrove tree, was inoculated with beneficial microbes in a nursery to assess any improvements in growth and biomass.From soil samples from the rhizosphere of B.sexangula in a mangrove forest in Panangadu of Kerala India, nitrogen-fixing bacteria Azotobacter chroococcum and Azospirillum brasilense were isolated.The phosphatesolubilising bacterium Bacillus megaterium and potassiummobilizing bacteria Frateruria aurantia were also isolated and cultured on suitable media.Later, ripe propagules of B.sexangula were collected from matured trees and raised in sterilized soil bags(13×25 cm) containing sterilized soil and sand(2:1 ratio).The cultured beneficial microbes were propagated and used to inoculate the ripe propagules of B.sexangula and maintained in the nursery for 6 months.After 6 months, growth and biomass of the inoculated propagules were greater than for the uninoculated control propagules.Shoot length, number of leaves, stem girth and root length were also significantly greater than in the controls.This study showed that the mangrove-specific beneficial microbes influenced the growth of B.sexangula in the nursery and will help in the establishment of B.sexangula in degraded mangrove forests.
基金Supported by Technological Department Momentous Item of Heilongjiang Province(GA06C101-07)
文摘It is shown by the result of the dual-cultured experiment that the inhibitory rate of DZW-47 was 60.42%,and the inhibitory rates of R.solani by actinomyces ZLR-2 and ZLR-11 were 43.75% and 43.05%,lower than that of DZW-47.The inhibitory mycelia growth mechanism of different strains on R.solani was quite different,with DZW-3 mainly on the aspect of hyperparasitism, DZW-21 on the synergism of hyperparasitism and metabolite,DZW-47 on the synergism of nutrient competition and secondary metabolite,ZLR-2 and ZLR- 11 on producing secondary metabolite.Controlling efficiency of seedling bed accorded basically with that of the broth.The controlling efficiency of DZW-47,ZLR-2,ZLR-11,DZW-21 and DZW-3 were 97.20%,95.7%,94.6%,93.6% and 89.20%,respectively.
