Purpose: To investigate the cytotoxicity of soft contact lens multi-purpose care solutions which are now in common use in China.Methods: The cell culture method was used. Cytotoxicity was indicated by significant incr...Purpose: To investigate the cytotoxicity of soft contact lens multi-purpose care solutions which are now in common use in China.Methods: The cell culture method was used. Cytotoxicity was indicated by significant increases in the number of dead cells relative to controls.Results: Cells were exposed to soft contact lens care solutions for 15 min. They were irregular in shape and variable in size. The intercellular space increased and variable in size.The in-tercelluar space increased and the cells became scrunken. With the time of exposure elongated , damage of cells became more severe.Conclusions: Four kinds of soft contact lens multi-purpose care solutions may have harmful effects on the culture of human corneal epithelial cells. Soaked lenses should be rinsed with saline before being placed in the eyes in order to reduce the potential toxicity of contact lens care solutions. Eye science 1998; 14 :45 - 47.展开更多
A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body.Although how cancer cells collectively migrate by following molecular rules which influence the stat...A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body.Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated,the impact of physical interactions on cell spreading remains less understood.Cumulative effects of physical interactions exist as the interplay between various physical parameters such as(1)tissue surface tension,(2)viscoelasticity caused by collective cell migration,and(3)solid stress accumulated in the cell aggregate core region.This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates.While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension,which directs cell movement from the surface to the core region of two-aggregate systems,cancer cells rather perform surface cell rearrangement.Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced.The solid stress,accumulated in the core region of the two-aggregate system,is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition;however,this stress enhances the movement of cancer cells.We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.展开更多
目的观察不同浓度条件下,细胞接触对骨髓间充质干细胞(BMSCs)分化为心肌细胞(CM)的诱导作用。方法利用表达绿色荧光蛋白(GFP)的转基因大鼠的BMSCs与CM以不同比例(1∶1、1∶10、1∶20、1∶40)共培养1周,分别采用免疫荧光染色及流式细胞...目的观察不同浓度条件下,细胞接触对骨髓间充质干细胞(BMSCs)分化为心肌细胞(CM)的诱导作用。方法利用表达绿色荧光蛋白(GFP)的转基因大鼠的BMSCs与CM以不同比例(1∶1、1∶10、1∶20、1∶40)共培养1周,分别采用免疫荧光染色及流式细胞计数分析检测肌钙蛋白Ⅰ(cTnI)、α-sarcom eric actin in和MEF-2C等心肌特异性蛋白,采用膜片钳技术检测分化细胞的电生理功能。结果在共培养条件下,部分GFP-BMSCs可表达一系列心肌表面标记物,如心肌特异性cTnI蛋白等,且获得心肌分化表型的数量随着CM数量的增加而增加。部分GFP-BMSCs与CM一起同步搏动,并可获得与CM相似的膜电位。结论细胞接触可诱导BMSCs获得有功能的心肌分化表型,其数量与心肌的数量不同浓度有相对的依赖性,随着心肌数量的增加而增加。展开更多
文摘Purpose: To investigate the cytotoxicity of soft contact lens multi-purpose care solutions which are now in common use in China.Methods: The cell culture method was used. Cytotoxicity was indicated by significant increases in the number of dead cells relative to controls.Results: Cells were exposed to soft contact lens care solutions for 15 min. They were irregular in shape and variable in size. The intercellular space increased and variable in size.The in-tercelluar space increased and the cells became scrunken. With the time of exposure elongated , damage of cells became more severe.Conclusions: Four kinds of soft contact lens multi-purpose care solutions may have harmful effects on the culture of human corneal epithelial cells. Soaked lenses should be rinsed with saline before being placed in the eyes in order to reduce the potential toxicity of contact lens care solutions. Eye science 1998; 14 :45 - 47.
基金This work was supported by the Ministry of Education,Science and Technological Development of The Republic of Serbia(Contract No.451-03-68/2022-14/200135).
文摘A key feature that distinguishes cancer cells from all other cells is their capability to spread throughout the body.Although how cancer cells collectively migrate by following molecular rules which influence the state of cell-cell adhesion contacts has been comprehensively formulated,the impact of physical interactions on cell spreading remains less understood.Cumulative effects of physical interactions exist as the interplay between various physical parameters such as(1)tissue surface tension,(2)viscoelasticity caused by collective cell migration,and(3)solid stress accumulated in the cell aggregate core region.This review aims to point out the role of these physical parameters in cancer cell spreading by considering and comparing the rearrangement of various mono-cultured cancer and epithelial model systems such as the fusion of two cell aggregates.While epithelial cells undergo volumetric cell rearrangement driven by the tissue surface tension,which directs cell movement from the surface to the core region of two-aggregate systems,cancer cells rather perform surface cell rearrangement.Cancer cells migrate toward the surface of the two-aggregate system driven by the solid stress while the surface tension is significantly reduced.The solid stress,accumulated in the core region of the two-aggregate system,is capable of suppressing the movement of epithelial cells that can undergo the jamming state transition;however,this stress enhances the movement of cancer cells.We have focused here on the multi-scale rheological modeling approaches that aimed at reproducing and understanding these biological systems.
文摘目的观察不同浓度条件下,细胞接触对骨髓间充质干细胞(BMSCs)分化为心肌细胞(CM)的诱导作用。方法利用表达绿色荧光蛋白(GFP)的转基因大鼠的BMSCs与CM以不同比例(1∶1、1∶10、1∶20、1∶40)共培养1周,分别采用免疫荧光染色及流式细胞计数分析检测肌钙蛋白Ⅰ(cTnI)、α-sarcom eric actin in和MEF-2C等心肌特异性蛋白,采用膜片钳技术检测分化细胞的电生理功能。结果在共培养条件下,部分GFP-BMSCs可表达一系列心肌表面标记物,如心肌特异性cTnI蛋白等,且获得心肌分化表型的数量随着CM数量的增加而增加。部分GFP-BMSCs与CM一起同步搏动,并可获得与CM相似的膜电位。结论细胞接触可诱导BMSCs获得有功能的心肌分化表型,其数量与心肌的数量不同浓度有相对的依赖性,随着心肌数量的增加而增加。
