喉癌是上呼吸道常见的恶性肿瘤,严重影响着人类的健康。研究表明热休克蛋白70(heat shock protein 70,HSP70)在正常组织中多不表达,但在包括头颈鳞状细胞癌在内的多种肿瘤组织却有异常表达。肿瘤细胞中高表达的HSP70,对细胞的增殖、侵...喉癌是上呼吸道常见的恶性肿瘤,严重影响着人类的健康。研究表明热休克蛋白70(heat shock protein 70,HSP70)在正常组织中多不表达,但在包括头颈鳞状细胞癌在内的多种肿瘤组织却有异常表达。肿瘤细胞中高表达的HSP70,对细胞的增殖、侵袭及转移、机体对肿瘤治疗耐受性的发生及肿瘤预后等方面均有很重要的作用[1]。RNA干扰(RNA interference,RNAi)技术是近年来发展起来的一项新的基因技术。展开更多
AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.``METHODS Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patien...AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.``METHODS Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods The presence of HCV RNA, the expression of HCV antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry respectively.``RESULTS The intracellular HCV RNA was first detected on d 2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. The expression of HCV NS3, CP10antigens could be observed in cells. The fresh cells could be infected by supematant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed.``CONCLUSION The hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro.展开更多
文摘喉癌是上呼吸道常见的恶性肿瘤,严重影响着人类的健康。研究表明热休克蛋白70(heat shock protein 70,HSP70)在正常组织中多不表达,但在包括头颈鳞状细胞癌在内的多种肿瘤组织却有异常表达。肿瘤细胞中高表达的HSP70,对细胞的增殖、侵袭及转移、机体对肿瘤治疗耐受性的发生及肿瘤预后等方面均有很重要的作用[1]。RNA干扰(RNA interference,RNAi)技术是近年来发展起来的一项新的基因技术。
基金Suppprted by the Mational Natural Science Foundation of China,No.39670672.
文摘AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.``METHODS Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods The presence of HCV RNA, the expression of HCV antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry respectively.``RESULTS The intracellular HCV RNA was first detected on d 2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. The expression of HCV NS3, CP10antigens could be observed in cells. The fresh cells could be infected by supematant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed.``CONCLUSION The hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro.
