[Objective] This study aimed to investigate the immunological adjuvant function of aluminium phosphate and chicken IL-18 in NDV F gene vaccine. [Method] The vaccine （0.2 ml） containing aluminum phosphate adjuvant （...[Objective] This study aimed to investigate the immunological adjuvant function of aluminium phosphate and chicken IL-18 in NDV F gene vaccine. [Method] The vaccine （0.2 ml） containing aluminum phosphate adjuvant （90 μg）, pcDNA/F （200μg）, and pcDNA/chlL-18 （200 μg） was prepared. The 7 d old chick- ens to be tested were randomly divided into six groups （12 chickens in each group） and immunized through intramuscular injection with inactivated Newcastle disease vaccines, pcDNA/F＋pcDNA/chlL-18＋phosphate aluminum, pcDNA/F, pcDNA/F.＋pcDNA/ chlL-18, pcDNA/F＋aluminum phosphate, and physiological saline respectively; the secondary immunization was conducted with the same dose when the chickens were 21 d old. Their blood was sampled 0, 7, 14, 21, 28 d after first immunization. Anti- body titer was detected with ELISA and T cell transformation rate was measured with MIT. Experimental chicken will be challenged with 30 LD50 NDV virulence 28 d after first immunization. [Result] The survival rate of the chickens immunized with pcDNA/F＋aluminium phosphate＋pcDNA/chlL-18 achieved 8/12, higher than that of those immunized with pcDNA/F 4/12 and pcDNA/F＋pcDNA/chlL-18 （6/12）. The NDV antibody titer of the chickens immunized with pcDNA/F＋ aluminum phosphate, pcD- NA/F＋pcDNA/chlL-18 and pcDNA/F＋pcDNA/chlL-18＋aluminum phosphate is not differ- ent （P〉0.05）, but significantly lower than that of the chickens immunized with tradi- tional vaccine （P〈0.05）. The T cell transformation rate of the chickens immunized with pcDNA/F＋pcDNA/chlL-18＋aluminium phosphate was obviously higher than that of the chickens immunized with pcDNA/F （P〈0.05）. The T cell transformation rates of chickens immunized with pcDNA/F and the traditional vaccine showed no signifi- cant difference （P〉0.05）. [Conclusion] Combination of aluminium phosphate and pcD- NA/chlL-18 can significantly enhance the immune effect of NDV F gene vaccine.展开更多
Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartil...Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartilage matrix synthesis. The current study was designed to investigate the associations of the BMP-2 gene polymorphism with chicken skeletal traits. Northeast Agricultural University F2 resource population was used in this study. Body weight and body composition traits were measured in F2 population. Polymorphism between parental lines was detected by DNA sequencing, and PCR-fragment length polymorphism method was then developed to screen the population. The results showed that the BMP-2 gene polymorphism was associated with skeletal traits in F2 population. This research suggests that BMP-2 gene may be a candidate locus or linked to a major gene that affects skeletal traits in chickens.展开更多
To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe ...To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe Yaoshan hens using PCR-SSCP （single-strand conformation polymorphism） and sequencing method, and analyzed the correlation between genetic variation and egg production traits. The results showed that TT and TG genotypes in mRNA SNlX）62 （C→T） loci of the DRD2 gene had extremely significant difference in egg production at 38 weeks age （P 〈0.01 ）, and significant difference in egg weight at 300 days age （P 〈0.05 ）. The single nucleotide polymorphisms （SNPs） mutation induced synonymous mutation of the 312th amino acids （leucine） in DRD2 protein, from L （CTG） to L （TI＇G）. The mRNA SNP962 （C→T） loci had a larger genetic effect on egg production at 38 weeks age, and could be used as a molecular marker in early breeding of Libo Yaoshan chicken.展开更多
[ Abstracts ] In order to investigate the effect of chicken Akirin2 gene on the immune response induced by VP2 DNA vaccine of infectious bursal disease virus （IBDV）. [ Methods] The 14-day-old SPF chickens were immun...[ Abstracts ] In order to investigate the effect of chicken Akirin2 gene on the immune response induced by VP2 DNA vaccine of infectious bursal disease virus （IBDV）. [ Methods] The 14-day-old SPF chickens were immunized with recombinant plasmids expressing VP2 protein and Akirin2 protein, and strength- ened immunization was conducted at the 14＇~ day after the first immunization. Finally, test chickens were challenged with IBDVBC6-85 virulent strain. [ Resultss ] Test results showed that Akirin2 gene could enhance the specific immune response induced by VP2 DNA vaccine, improve the proliferation of peripheral blood lym- phocytes and ＇affect the expressing of cytokines TNF-a, IFN-Y, IL-1β, IL-2, IL-4, IL 6, IL-9, IL-10, IL-17 and IL-18. Effects of recombinant plasmids co-ex- pressing Akirin2 protein and VP2 protein on cytokine expression showed some differences with the recombinant plasmids expressing Akirir/2 protein or VP2 protein along. [ Conclusions] Chicken Akirin2 gene could significantly enhance the humoral immune response and cellular immune response induced by VP2 DNA vaccine of IBDV.展开更多
An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><s...An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"><sup></sup></span><sup></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;"><sup>–ΔΔCт</sup><span style="white-space:nowrap;"></span>) was carried out for the determination of fold change. The fold change of spleen tissue expression at cytolytic immune response of the birds was 30,362.44 compared to latent response 294.07;and the fold change of thymus expression at cytolytic immune response of the birds was 51.98 compared to latent response 5.24</span><i><span style="font-family:Verdana;">. </span></i><span style="font-family:Verdana;">At both cytolytic and latent stages of immune response to SRBC antigen, <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> expression in the spleen was comparatively higher than in the thymus and the height of transcriptional activity was associated with the cytolytic stage</span><i><span style="font-family:Verdana;">. </span></i><span style="font-family:Verdana;">The birds of high titre at both the cytolytic and the latent responses had higher mRNA expression.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">This study concluded that <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene expression in the Nigerian indigenous chicken was induced at the cytolytic stage and repressed at the latent stage. During avian infections, the category of high immune response birds would perform better than the low immune response counterpart;and the protective response that <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene <span style="font-family:Verdana;">offers </span><span style="font-family:Verdana;">will be repressed from one time point to the other.</span>展开更多
文摘[Objective] This study aimed to investigate the immunological adjuvant function of aluminium phosphate and chicken IL-18 in NDV F gene vaccine. [Method] The vaccine （0.2 ml） containing aluminum phosphate adjuvant （90 μg）, pcDNA/F （200μg）, and pcDNA/chlL-18 （200 μg） was prepared. The 7 d old chick- ens to be tested were randomly divided into six groups （12 chickens in each group） and immunized through intramuscular injection with inactivated Newcastle disease vaccines, pcDNA/F＋pcDNA/chlL-18＋phosphate aluminum, pcDNA/F, pcDNA/F.＋pcDNA/ chlL-18, pcDNA/F＋aluminum phosphate, and physiological saline respectively; the secondary immunization was conducted with the same dose when the chickens were 21 d old. Their blood was sampled 0, 7, 14, 21, 28 d after first immunization. Anti- body titer was detected with ELISA and T cell transformation rate was measured with MIT. Experimental chicken will be challenged with 30 LD50 NDV virulence 28 d after first immunization. [Result] The survival rate of the chickens immunized with pcDNA/F＋aluminium phosphate＋pcDNA/chlL-18 achieved 8/12, higher than that of those immunized with pcDNA/F 4/12 and pcDNA/F＋pcDNA/chlL-18 （6/12）. The NDV antibody titer of the chickens immunized with pcDNA/F＋ aluminum phosphate, pcD- NA/F＋pcDNA/chlL-18 and pcDNA/F＋pcDNA/chlL-18＋aluminum phosphate is not differ- ent （P〉0.05）, but significantly lower than that of the chickens immunized with tradi- tional vaccine （P〈0.05）. The T cell transformation rate of the chickens immunized with pcDNA/F＋pcDNA/chlL-18＋aluminium phosphate was obviously higher than that of the chickens immunized with pcDNA/F （P〈0.05）. The T cell transformation rates of chickens immunized with pcDNA/F and the traditional vaccine showed no signifi- cant difference （P〉0.05）. [Conclusion] Combination of aluminium phosphate and pcD- NA/chlL-18 can significantly enhance the immune effect of NDV F gene vaccine.
基金Supported by National "973" Project of China (2006CB102105)National "863" Project of China (2006AA10A120)Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-42-G1-07)
文摘Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartilage matrix synthesis. The current study was designed to investigate the associations of the BMP-2 gene polymorphism with chicken skeletal traits. Northeast Agricultural University F2 resource population was used in this study. Body weight and body composition traits were measured in F2 population. Polymorphism between parental lines was detected by DNA sequencing, and PCR-fragment length polymorphism method was then developed to screen the population. The results showed that the BMP-2 gene polymorphism was associated with skeletal traits in F2 population. This research suggests that BMP-2 gene may be a candidate locus or linked to a major gene that affects skeletal traits in chickens.
基金Supported by Breeding and Demonstration Promotion of New Yaoshan Chicken Strains(QNYZ[2014]008)Key Gene Screening of Broodiness Breeding of Yaoshan Chicken(QKHJ[2014]2012)Doctoral Starting up Foundation of Guizhou Institute of Animal Husbandry and Veterinary in 2014:STAT5Gene Mutation Detection of Yaoshan Chicken and Associated Analysis with Egg Production Traits&CDS Region’s Cloning and Differential Expression of ONECUT1 Gene in Yaoshan Chicken and Associated Analysis with Production Traits(QKH LH[2015]7067)
文摘To improve egg production performance of local chicken breed in Guizhou Province, Libo Yaoshan chicken, with dopamine receptor 2 （ DRD2 ） as one of the candidate genes, we detected its genetic variation in 196 Libe Yaoshan hens using PCR-SSCP （single-strand conformation polymorphism） and sequencing method, and analyzed the correlation between genetic variation and egg production traits. The results showed that TT and TG genotypes in mRNA SNlX）62 （C→T） loci of the DRD2 gene had extremely significant difference in egg production at 38 weeks age （P 〈0.01 ）, and significant difference in egg weight at 300 days age （P 〈0.05 ）. The single nucleotide polymorphisms （SNPs） mutation induced synonymous mutation of the 312th amino acids （leucine） in DRD2 protein, from L （CTG） to L （TI＇G）. The mRNA SNP962 （C→T） loci had a larger genetic effect on egg production at 38 weeks age, and could be used as a molecular marker in early breeding of Libo Yaoshan chicken.
基金Supported by Guangdong Province Application of Science and Technology Research and Development of Special Funds(2015B020230011)
文摘[ Abstracts ] In order to investigate the effect of chicken Akirin2 gene on the immune response induced by VP2 DNA vaccine of infectious bursal disease virus （IBDV）. [ Methods] The 14-day-old SPF chickens were immunized with recombinant plasmids expressing VP2 protein and Akirin2 protein, and strength- ened immunization was conducted at the 14＇~ day after the first immunization. Finally, test chickens were challenged with IBDVBC6-85 virulent strain. [ Resultss ] Test results showed that Akirin2 gene could enhance the specific immune response induced by VP2 DNA vaccine, improve the proliferation of peripheral blood lym- phocytes and ＇affect the expressing of cytokines TNF-a, IFN-Y, IL-1β, IL-2, IL-4, IL 6, IL-9, IL-10, IL-17 and IL-18. Effects of recombinant plasmids co-ex- pressing Akirin2 protein and VP2 protein on cytokine expression showed some differences with the recombinant plasmids expressing Akirir/2 protein or VP2 protein along. [ Conclusions] Chicken Akirin2 gene could significantly enhance the humoral immune response and cellular immune response induced by VP2 DNA vaccine of IBDV.
文摘An economically-important trait in poultry for which gene identification <span style="font-family:Verdana;">continues to be a challenge is immune response. The objective of the study </span><span style="font-family:Verdana;">was</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">to quantitate the expression of major histocompatibility complex (MHC) class II <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene at cytolytic and latent immune response stages in Nigerian indigenous chickens. </span><span style="font-family:Verdana;">A total of 108 Nigerian indigenous chickens (NIC) were sourced across the South-western states in Nigeria. The birds were inoculated with sheep red blood cells (SRBC), after which blood samples were obtained (5 days post-inoculation) and antibody haemagglutination test was carried out to place the birds into groups of high and low antibody titre levels.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">The categorisation of the birds resulted in six groups of normal feather high, normal feather low, naked neck high, naked neck low, frizzle feather high and frizzle feather low antibody groups. </span><span style="font-family:Verdana;">A total of 48 chicks w</span></span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> selected from the progeny for gene expression studies. </span><span style="font-family:Verdana;">Surgical excision of thymus and spleen was carried out for the detection of cytolytic and latent responses of the birds. β-actin was used as the endogenous control and the critical threshold method</span><span> </span><span style="font-family:Verdana;">(2<span style="white-space:nowrap;"><sup></sup></span><sup></sup></span><span style="font-family:;" "=""><span style="font-family:Verdana;"><sup>–ΔΔCт</sup><span style="white-space:nowrap;"></span>) was carried out for the determination of fold change. The fold change of spleen tissue expression at cytolytic immune response of the birds was 30,362.44 compared to latent response 294.07;and the fold change of thymus expression at cytolytic immune response of the birds was 51.98 compared to latent response 5.24</span><i><span style="font-family:Verdana;">. </span></i><span style="font-family:Verdana;">At both cytolytic and latent stages of immune response to SRBC antigen, <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> expression in the spleen was comparatively higher than in the thymus and the height of transcriptional activity was associated with the cytolytic stage</span><i><span style="font-family:Verdana;">. </span></i><span style="font-family:Verdana;">The birds of high titre at both the cytolytic and the latent responses had higher mRNA expression.</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">This study concluded that <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene expression in the Nigerian indigenous chicken was induced at the cytolytic stage and repressed at the latent stage. During avian infections, the category of high immune response birds would perform better than the low immune response counterpart;and the protective response that <i></i></span><i><i><span style="font-family:Verdana;">BLB2</span></i><span style="font-family:Verdana;"></span></i> gene <span style="font-family:Verdana;">offers </span><span style="font-family:Verdana;">will be repressed from one time point to the other.</span>
