目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清...目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。展开更多
A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarith...A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarithmic Maximum A Posterior (PSW-log-MAP), the on-chip turbo decoder can decode an information bit by only an average of two clocks per iteration. On the other hand, a high-parallel pipeline Viterbi algorithm is adopted to realize the 256-state convolutional code decoding. The final decoder with an 8×chip-clock (30 72MHz) driving can concurrently process a data rate up to 2 5Mbps of turbo coded sequences and a data rate over 400kbps of convolutional codes. There is no extern memory needed. Test results show that the decoding performance is only 0 2～0 3dB or less lost comparing to float simulation.展开更多
文章提出一种在片上系统(System on Chip,SoC)实现高吞吐率的有限状态熵编码(finite state entropy,FSE)算法。通过压缩率、速度、资源消耗、功耗4个方面对所提出的编码器和解码器与典型的硬件哈夫曼编码(Huffman coding,HC)进行性能比...文章提出一种在片上系统(System on Chip,SoC)实现高吞吐率的有限状态熵编码(finite state entropy,FSE)算法。通过压缩率、速度、资源消耗、功耗4个方面对所提出的编码器和解码器与典型的硬件哈夫曼编码(Huffman coding,HC)进行性能比较,结果表明,所提出的硬件FSE编码器和解码器具有显著优势。硬件FSE(hFSE)架构实现在SoC的处理系统和可编程逻辑块(programmable logic,PL)上,通过高级可扩展接口(Advanced eXtensible Interface 4,AXI4)总线连接SoC的处理系统和可编程逻辑块。算法测试显示,FSE算法在非均匀数据分布和大数据量情况下,具有更好的压缩率。该文设计的编码器和解码器已在可编程逻辑块上实现,其中包括1个可配置的缓冲模块,将比特流作为单字节或双字节配置输出到8 bit位宽4096深度或16 bit位宽2048深度的块随机访问存储器(block random access memory,BRAM)中。所提出的FSE硬件架构为实时压缩应用提供了高吞吐率、低功耗和低资源消耗的硬件实现。展开更多
The understanding of cSNPs of cancer-related genes harboring in high frequency loss regions of tumor chromosomes can advance the disclosure of genetic and variant mechanisms of tumorigenesis,and the investigation of c...The understanding of cSNPs of cancer-related genes harboring in high frequency loss regions of tumor chromosomes can advance the disclosure of genetic and variant mechanisms of tumorigenesis,and the investigation of cancer susceptibility. In preparing a gene chip for detecting polymorphisms on coding region of genes in hepatocellular carcinoma tissues, some cSNPs are of interest for their potential links with phenotype. METHODS: The genes harboring in loss regions with high frequency of hepatocellular carcinoma (HCC) were selected, the related information of cSNP sequences was obtained from the SNP database (dbSNP) of the National Center for Biotechnology Information (NCBI). Then appropriate primers and oligonucleotide probes were designed according to the SNP sites, and a gene chip for the detection of SNPs was constructed. The chip included 48 cSNPs of 25 hepatocellular carcinoma-related genes. The PCR products labeled by Dig-dUTP were hybridized with the cSNP chip. RESULTS:The sensitivity, influence by probe concentration, and reiteration of the chip were detected, with a high sensitivity of 6 × 10-3 ng/μl. The signal of hybridization was reduced with a lower concentration of probe. Seven polymorphisms of caspase 9 (rs2308941) C →T and DOK2 (rs2242241) T→G, 6 of polymorphisms of EGFL3 (rs947345) A→G, caspase 9 (rs2308938) C→G and PHGDH (rs1801955)T→A, 5 of polymorphisms of E2F2(rs3218170) G→A,4 of polymorphisms of MUTYH( rs1140507) T→C and BNIP3L(rs1055806)G→T, and 1 of polymorphism of TNFRSF1B (rs1061622)T→G were detected by the chip in the tissues of 10 HCC. Samples of caspase 9 (rs2308941G) and (rs2308941A) were verified by PCR-SSCP and sequencing. CONCLUSION:The cSNP chip of hepatocellular carcinoma-related genes can accelerate the discovery of polymorphic markers on hepatocellular carcinoma.展开更多
Generalized Bent function and generalized Bent function sequences are introduced in this paper.The main performance or these sequences used as SW/SFH(Short Wave/Slow Frequency Hopping) code are studied. And the hardwa...Generalized Bent function and generalized Bent function sequences are introduced in this paper.The main performance or these sequences used as SW/SFH(Short Wave/Slow Frequency Hopping) code are studied. And the hardware circuit and the soflware program flow chart of the SW/SFH PN code generator are also given,which is based on generalized Bent function sequence generator by using a single chip mlcrocomputer.展开更多
A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC...A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC）, moving target detection （MTD）, constant false alarm rate （CFAR） and target dots processing. Preliminary target dots information is obtained in PC, MTD, and CFAR modules and Nios I! CPU is used for target dots combination and false sidelobe target removing. Sys- tem on programmable chip （SOPC） technique is adopted in the system in which SDRAM is used to cache data. Finally, a FPGA-based binary phase coded radar signal processor is realized and simula- tion result is given.展开更多
A modiΡed pseudo-noise（PN） code regeneration method is proposed to improve the clock tracking accuracy without impairing the code acquisition time performance.Thus,the method can meet the requirement of high accura...A modiΡed pseudo-noise（PN） code regeneration method is proposed to improve the clock tracking accuracy without impairing the code acquisition time performance.Thus,the method can meet the requirement of high accuracy ranging measurements in short time periods demanded by radio-science missions.The tracking error variance is derived by linear analysis.For some existing PN codes,which can be acquired rapidly,the tracking error variance performance of the proposed method is about 2.6 dB better than that of the JPL scheme（originally proposed by Jet Propulsion Laboratory）,and about 1.5 dB better than that of the traditional double loop scheme.展开更多
文摘目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。
文摘A memory and driving clock efficient design scheme to achieve WCDMA high-speed channel decoder on a single XILINX’ XVC1000E FPGA chip is presented. Using a modified MAP algorithm, say parallel Sliding Window logarithmic Maximum A Posterior (PSW-log-MAP), the on-chip turbo decoder can decode an information bit by only an average of two clocks per iteration. On the other hand, a high-parallel pipeline Viterbi algorithm is adopted to realize the 256-state convolutional code decoding. The final decoder with an 8×chip-clock (30 72MHz) driving can concurrently process a data rate up to 2 5Mbps of turbo coded sequences and a data rate over 400kbps of convolutional codes. There is no extern memory needed. Test results show that the decoding performance is only 0 2～0 3dB or less lost comparing to float simulation.
文摘文章提出一种在片上系统(System on Chip,SoC)实现高吞吐率的有限状态熵编码(finite state entropy,FSE)算法。通过压缩率、速度、资源消耗、功耗4个方面对所提出的编码器和解码器与典型的硬件哈夫曼编码(Huffman coding,HC)进行性能比较,结果表明,所提出的硬件FSE编码器和解码器具有显著优势。硬件FSE(hFSE)架构实现在SoC的处理系统和可编程逻辑块(programmable logic,PL)上,通过高级可扩展接口(Advanced eXtensible Interface 4,AXI4)总线连接SoC的处理系统和可编程逻辑块。算法测试显示,FSE算法在非均匀数据分布和大数据量情况下,具有更好的压缩率。该文设计的编码器和解码器已在可编程逻辑块上实现,其中包括1个可配置的缓冲模块,将比特流作为单字节或双字节配置输出到8 bit位宽4096深度或16 bit位宽2048深度的块随机访问存储器(block random access memory,BRAM)中。所提出的FSE硬件架构为实时压缩应用提供了高吞吐率、低功耗和低资源消耗的硬件实现。
文摘The understanding of cSNPs of cancer-related genes harboring in high frequency loss regions of tumor chromosomes can advance the disclosure of genetic and variant mechanisms of tumorigenesis,and the investigation of cancer susceptibility. In preparing a gene chip for detecting polymorphisms on coding region of genes in hepatocellular carcinoma tissues, some cSNPs are of interest for their potential links with phenotype. METHODS: The genes harboring in loss regions with high frequency of hepatocellular carcinoma (HCC) were selected, the related information of cSNP sequences was obtained from the SNP database (dbSNP) of the National Center for Biotechnology Information (NCBI). Then appropriate primers and oligonucleotide probes were designed according to the SNP sites, and a gene chip for the detection of SNPs was constructed. The chip included 48 cSNPs of 25 hepatocellular carcinoma-related genes. The PCR products labeled by Dig-dUTP were hybridized with the cSNP chip. RESULTS:The sensitivity, influence by probe concentration, and reiteration of the chip were detected, with a high sensitivity of 6 × 10-3 ng/μl. The signal of hybridization was reduced with a lower concentration of probe. Seven polymorphisms of caspase 9 (rs2308941) C →T and DOK2 (rs2242241) T→G, 6 of polymorphisms of EGFL3 (rs947345) A→G, caspase 9 (rs2308938) C→G and PHGDH (rs1801955)T→A, 5 of polymorphisms of E2F2(rs3218170) G→A,4 of polymorphisms of MUTYH( rs1140507) T→C and BNIP3L(rs1055806)G→T, and 1 of polymorphism of TNFRSF1B (rs1061622)T→G were detected by the chip in the tissues of 10 HCC. Samples of caspase 9 (rs2308941G) and (rs2308941A) were verified by PCR-SSCP and sequencing. CONCLUSION:The cSNP chip of hepatocellular carcinoma-related genes can accelerate the discovery of polymorphic markers on hepatocellular carcinoma.
文摘Generalized Bent function and generalized Bent function sequences are introduced in this paper.The main performance or these sequences used as SW/SFH(Short Wave/Slow Frequency Hopping) code are studied. And the hardware circuit and the soflware program flow chart of the SW/SFH PN code generator are also given,which is based on generalized Bent function sequence generator by using a single chip mlcrocomputer.
基金Supported by the Ministerial Level Advanced Research Foundation (SP240012)
文摘A flexible field programmable gate array based radar signal processor is presented. The radar signal processor mainly consists of five functional modules： radar system timer, binary phase coded pulse compression（PC）, moving target detection （MTD）, constant false alarm rate （CFAR） and target dots processing. Preliminary target dots information is obtained in PC, MTD, and CFAR modules and Nios I! CPU is used for target dots combination and false sidelobe target removing. Sys- tem on programmable chip （SOPC） technique is adopted in the system in which SDRAM is used to cache data. Finally, a FPGA-based binary phase coded radar signal processor is realized and simula- tion result is given.
基金supported by the National Natural Science Foundation of China (60904090)the Postdoctoral Science Foundation of China(20080431306)the Special Postdoctoral Science Foundation of China (20081458)
文摘A modiΡed pseudo-noise（PN） code regeneration method is proposed to improve the clock tracking accuracy without impairing the code acquisition time performance.Thus,the method can meet the requirement of high accuracy ranging measurements in short time periods demanded by radio-science missions.The tracking error variance is derived by linear analysis.For some existing PN codes,which can be acquired rapidly,the tracking error variance performance of the proposed method is about 2.6 dB better than that of the JPL scheme（originally proposed by Jet Propulsion Laboratory）,and about 1.5 dB better than that of the traditional double loop scheme.
