Based on the analysis of the existing teaching situation of the“Construction Engineering Regulations”course,this paper divides the course content into three parts according to the course characteristics and content,...Based on the analysis of the existing teaching situation of the“Construction Engineering Regulations”course,this paper divides the course content into three parts according to the course characteristics and content,and explores three corresponding teaching modes.The proportion of student-led relationships in the three teaching modes is 80%,60%,and 90%,respectively,realizing a teaching mechanism centered on students and stimulating students’interest in independent learning.Teaching methods such as problem-oriented learning,group discussion,student reporting,MOOC(massive open online course),case analysis,etc.,have been used to establish a variety of comprehensive examination mechanisms such as quiz games,follow-up tests,and work displays.Practice has shown that after adopting these three teaching modes,classroom teaching efficiency has significantly improved,and students’abilities in exploration,expression,innovation,and team cooperation have also been enhanced.展开更多
Vocational education is an important way in the current talent training.It shoulders the heavy responsibility of transporting talents for the society.With the acceleration of urbanization construction and the developm...Vocational education is an important way in the current talent training.It shoulders the heavy responsibility of transporting talents for the society.With the acceleration of urbanization construction and the development of industrial technology,the demand for High Tech Talents in construction projects is constantly increasing.It is necessary to strengthen the improvement of vocational education in construction engineering.This paper mainly analyzes the mode of vocational education in construction engineering.展开更多
The development of urbanization has led to an increase in the number and scale of construction projects and the types of building construction engineering are getting advance and diverse due to the rapid development o...The development of urbanization has led to an increase in the number and scale of construction projects and the types of building construction engineering are getting advance and diverse due to the rapid development of technology.One of them is the static pressure prestressed pipe pile which is the most commonly used technology in modern building construction work.It is mainly used for pile foundation in construction work,and it has the advantages in less pollution,low noise,and high efficiency compared to the traditional pile foundation.Study on the characteristics of static pressure prestressed pipe pile must be carried out and strengthened the research to increase the effectiveness and quality of static pressure prestressed pipe pile on construction works.This paper is mainly to analyzed the characteristic and construction technology of static pressure prestressed pipe piles on building construction work.展开更多
Given the advances in science and technology,rapid development of socialist market economy and continuous advance of urbanization,it is necessary to enlarge the scale of engineering construction.As the form of enginee...Given the advances in science and technology,rapid development of socialist market economy and continuous advance of urbanization,it is necessary to enlarge the scale of engineering construction.As the form of engineering structure becomes more complex,large-scale and high-level projects with deep foundation have appeared in engineering construction.For construction engineering,one of its technologies includes solving the difficulties in construction.It is required to deal with the safety risk of construction in time to guarantee safety construction,timely solve the management difficulties and contradictory problems of the project and ensure both the safety of engineering construction and the rationalization of the institution setting of the safety supervision on the project.展开更多
In today’s comprehensive development of the domestic economy,China’s construction industry has reaped remarkable achievements.As a very important composition in the field of construction in China,the characteristics...In today’s comprehensive development of the domestic economy,China’s construction industry has reaped remarkable achievements.As a very important composition in the field of construction in China,the characteristics of concrete engineering itself determines that it is prone to encounter a variety of problems,the most frequent of which is various cracks.Cracks not only affect the aesthetics of the building,but also affect the durability and safety of the project.Considering these potential hazards,the causes of concrete cracks must be carefully analyzed to control and improve them in an effective way to ensure the safety and stability of concrete buildings.展开更多
In recent years,China has made significant progress in the construction of highways,resulting in an improved highway network that has provided robust support for economic and social development.However,the rapid expan...In recent years,China has made significant progress in the construction of highways,resulting in an improved highway network that has provided robust support for economic and social development.However,the rapid expansion of highway construction,power supply,and distribution has led to several challenges in mechanical and electrical engineering technology.Ensuring the safe,stable,and cost-effective operation of the power supply and distribution system to meet the diverse requirements of highway operations has become a pressing issue.This article takes an example of a highway electromechanical engineering power supply and distribution construction project to provide insight into the construction process of highway electromechanical engineering power supply and distribution technology.展开更多
With the rapid development of information technology,the demand for talents in the field of software engineering is growing.In order to cultivate high-quality software engineering talents who meet the market demand,un...With the rapid development of information technology,the demand for talents in the field of software engineering is growing.In order to cultivate high-quality software engineering talents who meet the market demand,universities have continuously carried out the construction of software engineering majors.Accreditation Board for Engineering and Technology(ABET)certification,as an internationally recognized higher education quality assurance system,provides important reference and guidance for the construction of software engineering majors.Guided by student learning outcomes and core competencies,combined with the characteristics of software engineering talent cultivation,the innovation of talent cultivation mode takes industry-education integration and school-enterprise cooperation as the main development paths and explores comprehensive reform of the major in terms of professional positioning and goals,curriculum system,teaching conditions,and teachers.This comprehensive reform model has effectively promoted the development of major construction and improved the quality of talent cultivation.展开更多
To better improve the electrical safety of buildings and fulfill the role of lightning protection and grounding,this article combines cases and discusses seven aspects of lightning protection devices’construction tec...To better improve the electrical safety of buildings and fulfill the role of lightning protection and grounding,this article combines cases and discusses seven aspects of lightning protection devices’construction technology.These aspects include the lightning protection classification of buildings,air terminals,down conductors,grounding devices,division of lightning protection zones,and lightning electromagnetic pulse shielding.Through the introduction of this article,readers can gain a comprehensive understanding of the application and value of lightning protection grounding construction technology in building electrical installation projects.展开更多
The purpose of this paper is to discuss the integration of the elements of civic and political education into the engineering geology course to improve students’ideological and moral qualities.It is proposed that by ...The purpose of this paper is to discuss the integration of the elements of civic and political education into the engineering geology course to improve students’ideological and moral qualities.It is proposed that by integrating elements of civic and political education,students are guided to form a positive attitude toward engineering practice as well as correct values and ethics.With regard to the teaching design and implementation of the course,the implementation paths of teacher team building,careful teaching design,innovative teaching methods,and the integration of civic and politics in practical teaching are proposed to summarize the significance of integrating the elements of civic and political education in the construction of the engineering geology course.It is pointed out that this integration not only improves the quality of the course,but also provides a reference for the civic and political education of other similar professional courses.This integration not only focuses on the teaching of professional knowledge,but also pays more attention to the cultivation of students’ideology and morality,which provides a model and guidance for shaping new talents with all-round development.展开更多
We focus on the goal of undergraduate talent training,consider the course features of Road Engineering Construction Technology and Organization,analyze the problems existing in the practical teaching of the course,use...We focus on the goal of undergraduate talent training,consider the course features of Road Engineering Construction Technology and Organization,analyze the problems existing in the practical teaching of the course,use the advantages of BIM+virtual simulation technology,design a scientific and reasonable practical teaching content of Road Engineering Construction Technology and Organization,and address the contrast between the strong practical aspect of the traditional Road Engineering Construction Technology and Organization course and the lack of practical instruments in hope to improve students’learning autonomy,enhance the quality of practical teaching,achieve the training objectives of the course,and nurture applied technical talents.展开更多
Targeted treatment of cancer with monoclonal antibodies increases the benefit for patients. In order to improve the anti-tumor activity of monoclonal antibodies, multi-specific antibodies have entered the research fie...Targeted treatment of cancer with monoclonal antibodies increases the benefit for patients. In order to improve the anti-tumor activity of monoclonal antibodies, multi-specific antibodies have entered the research field. The emergence of various techniques to produce multi-specific recombinant antibody molecules has led to the selection of target combinations in various forms. To date, only a few multi-specific constructs have entered phase III clinical trials, in contrast to classical monoclonal antibodies. Some of the format options are outlined from a technical point of view. We focus on the achievements and prospects of the underlying technologies for generating biand multispecific antibodies.展开更多
The design process of the built environment relies on the collaborative effort of all parties involved in the project.During the design phase,owners,end users,and their representatives are expected to make the most cr...The design process of the built environment relies on the collaborative effort of all parties involved in the project.During the design phase,owners,end users,and their representatives are expected to make the most critical design and budgetary decisions-shaping the essential traits of the project,hence emerge the need and necessity to create and integrate mechanisms to support the decision-making process.Design decisions should not be based on assumptions,past experiences,or imagination.An example of the numerous problems that are a result of uninformed design decisions is“change orders”,known as the deviation from the original scope of work,which leads to an increase of the overall cost,and changes to the construction schedule of the project.The long-term aim of this inquiry is to understand the user’s behavior,and establish evidence-based control measures,which are actions and processes that can be implemented in practice to decrease the volume and frequency of the occurrence of change orders.The current study developed a foundation for further examination by proposing potential control measures,and testing their efficiency,such as integrating Virtual Reality(VR).The specific aim was to examine the effect of different visualization methods(i.e.,VR vs.construction drawings)on,(1)how well the subjects understand the information presented about the future/planned environment;(2)the subjects’perceived confidence in what the future environment will look like;(3)the likelihood of changing the built environment;(4)design review time;and(5)accuracy in reviewing and understanding the design.展开更多
With the introduction of“new engineering,new medical science,new agricultural science,and new liberal arts”construction,the reform of college English teaching is also imperative.Scholars nationwide have conducted at...With the introduction of“new engineering,new medical science,new agricultural science,and new liberal arts”construction,the reform of college English teaching is also imperative.Scholars nationwide have conducted attempts in different aspects of college English teaching and produced fruitful results.Yet,more efforts should be made.This paper discusses the reforms made by Wuhan University of Technology,focusing on the reformation of curriculum.展开更多
Software engineering is an engineering discipline that guides software developers to carry out software development,software maintenance,and software management.The traditional evaluation methods of software engineeri...Software engineering is an engineering discipline that guides software developers to carry out software development,software maintenance,and software management.The traditional evaluation methods of software engineering courses do not highlight the training goal of outcomebased education(OBE).This paper systematically studies the evaluation methods for practical courses in software engineering from three aspects:course evaluation guidelines,course evaluation methods and course evaluation effects;establishes a comprehensive,scientific,and objective system of course evaluation;effectively measures students’learning effect;promotes teachers to continuously improve the teaching process;and thus improves the teaching quality of software engineering courses.Besides the general approach,this paper also takes software project construction practice as an example to demonstrate the effect of the proposed approach.展开更多
Interface engineering can improve the charge separation efficiency and inhibit photocorrosion is an emerging direction of developing more efficient and cost-effective photocatalytic systems.Herein,we report the sulfur...Interface engineering can improve the charge separation efficiency and inhibit photocorrosion is an emerging direction of developing more efficient and cost-effective photocatalytic systems.Herein,we report the sulfur-confined intimate Cd S intergrown Cd(Cd S/Cd)Ohmic junction(peanut-chocolate-ball like)for high-efficient H2production with superior anti-photocorrosion ability,which was fabricated from in-situ photoreduction of CdS intergrown Cd2SO4(OH)2(CdS/Cd2SO4(OH)2)prepared through a facile space-controlled-solvothermal method.The ratios of CdS/Cd can be effectively controlled by tunning that of CdS/Cd2SO4(OH)2which were prepared by adjusting the volume of reaction liquid and the remaining space of the reactor.Experiments investigations and density functional theory(DFT)calculations reveal that the Cd S intergrown Cd Ohmic junction interfaces(with appropriate content Cd intergrown on Cd S(19.54 wt%))are beneficial in facilitating the transfer of photogenerated electrons by constructing an interfacial electric field and forming sulfur-confined structures for preventing the positive holes(h+)oxidize the Cd S.This contributes to a high photocatalytic H2production activity of 95.40μmol h-1(about 32.3 times higher than bare Cd S)and possesses outstanding photocatalytic stability over 205 h,much longer than most Cd S-based photocatalysts previously reported.The interface engineering design inspired by the structure of peanut-chocolate-ball can greatly promote the future development of catalytic systems for wider application.展开更多
Mesaconic acid has a special chemical structure and can undergo a series of reactions such as polymerization and addition. It is an important chemical intermediate and widely used in material, chemical and other indus...Mesaconic acid has a special chemical structure and can undergo a series of reactions such as polymerization and addition. It is an important chemical intermediate and widely used in material, chemical and other industries. The chemical synthesis of mesaconic acid requires nitric acid, which is dangerous and harmful to the environment. The production of mesaconic acid by microbial fermentation has the characteristics of low raw material price, high efficiency and strong specificity, and thus a strong industrial application prospect. Mesaconic acid is an intermediate product of glutamic acid degradation pathway of microorganisms such as Clostridium tetani. However, at present, few reports have been conducted on the production of mesaconic acid by metabolic engineering microorganisms. In this study, glutamate mutase(GLM) and 3-methylaspartate ammonialyase(MAL) from C. tetani were recombined and expressed in Escherichia coli, and the obtained strain, BL21(DE3)/pETDuet-1-MAL-mutS-mutE, achieved the yield of mesaconic acid of 1.06 g/L. Compared with the wild type, the yields of mesaconic acid from mutants G133A and G133S increased by 21% and 16%, respectively. After 24 h of flask fermentation, the yields of mesaconic acid reached 1.28 and 1.23 g/L, respectively. This study can provide reference for microbial synthesis of mesaconic acid.展开更多
Environmental Microbiology is a professional basic course in the curriculum system of Environmental and Ecological Engineering major,which follows the requirements of ideological and political theories teaching in the...Environmental Microbiology is a professional basic course in the curriculum system of Environmental and Ecological Engineering major,which follows the requirements of ideological and political theories teaching in the course in higher education,and should be implemented in every course.Therefore,the construction of ideological and political theories teaching in the course should be an important task in the course construction of Environmental Microbiology.In view of the curriculum characteristics of Environmental Microbiology,such as multi-disciplinary feature,wide coverage and rapid development,it is an effective method for the construction of ideological and political theories teaching in the course of Environmental Microbiology by fully tapping the ideological and political elements of the curriculum,such as patriotism,scientific spirit and practical innovation,and integrating the ideological and political theories teaching in the course into the whole curriculum design and classroom teaching construction.展开更多
With the development of China's economy,the implementation scale and scope of hydraulic engineering projects are increasing,and the application of anti-seepage technology has an important influence on the construc...With the development of China's economy,the implementation scale and scope of hydraulic engineering projects are increasing,and the application of anti-seepage technology has an important influence on the construction quality of hydraulic engineering.In this paper,the significance and problems of the application of anti-seepage technology in the construction of hydraulic engineering projects were mainly analyzed,and specific application strategies were proposed.展开更多
Municipal civil engineering is the key content of municipal construction,and the construction scale is usually large.The quality of the project plays an important role in the development of urban economy.Due to the ra...Municipal civil engineering is the key content of municipal construction,and the construction scale is usually large.The quality of the project plays an important role in the development of urban economy.Due to the rapid increase of high-rise buildings,skyscrapers and underground buildings,the construction technology of deep foundation pit support has gradually become an indispensable construction technology.Therefore,the selection of foundation pit support construction technology is crucial in ensuring that whether the foundation is firm and stable,and whether the subsequent construction activities can be carried out smoothly.In view of this,the article discusses the application of deep foundation pit support construction technology in municipal civil engineering,aiming to provide reference for subsequent projects.展开更多
文摘Based on the analysis of the existing teaching situation of the“Construction Engineering Regulations”course,this paper divides the course content into three parts according to the course characteristics and content,and explores three corresponding teaching modes.The proportion of student-led relationships in the three teaching modes is 80%,60%,and 90%,respectively,realizing a teaching mechanism centered on students and stimulating students’interest in independent learning.Teaching methods such as problem-oriented learning,group discussion,student reporting,MOOC(massive open online course),case analysis,etc.,have been used to establish a variety of comprehensive examination mechanisms such as quiz games,follow-up tests,and work displays.Practice has shown that after adopting these three teaching modes,classroom teaching efficiency has significantly improved,and students’abilities in exploration,expression,innovation,and team cooperation have also been enhanced.
文摘Vocational education is an important way in the current talent training.It shoulders the heavy responsibility of transporting talents for the society.With the acceleration of urbanization construction and the development of industrial technology,the demand for High Tech Talents in construction projects is constantly increasing.It is necessary to strengthen the improvement of vocational education in construction engineering.This paper mainly analyzes the mode of vocational education in construction engineering.
文摘The development of urbanization has led to an increase in the number and scale of construction projects and the types of building construction engineering are getting advance and diverse due to the rapid development of technology.One of them is the static pressure prestressed pipe pile which is the most commonly used technology in modern building construction work.It is mainly used for pile foundation in construction work,and it has the advantages in less pollution,low noise,and high efficiency compared to the traditional pile foundation.Study on the characteristics of static pressure prestressed pipe pile must be carried out and strengthened the research to increase the effectiveness and quality of static pressure prestressed pipe pile on construction works.This paper is mainly to analyzed the characteristic and construction technology of static pressure prestressed pipe piles on building construction work.
文摘Given the advances in science and technology,rapid development of socialist market economy and continuous advance of urbanization,it is necessary to enlarge the scale of engineering construction.As the form of engineering structure becomes more complex,large-scale and high-level projects with deep foundation have appeared in engineering construction.For construction engineering,one of its technologies includes solving the difficulties in construction.It is required to deal with the safety risk of construction in time to guarantee safety construction,timely solve the management difficulties and contradictory problems of the project and ensure both the safety of engineering construction and the rationalization of the institution setting of the safety supervision on the project.
文摘In today’s comprehensive development of the domestic economy,China’s construction industry has reaped remarkable achievements.As a very important composition in the field of construction in China,the characteristics of concrete engineering itself determines that it is prone to encounter a variety of problems,the most frequent of which is various cracks.Cracks not only affect the aesthetics of the building,but also affect the durability and safety of the project.Considering these potential hazards,the causes of concrete cracks must be carefully analyzed to control and improve them in an effective way to ensure the safety and stability of concrete buildings.
文摘In recent years,China has made significant progress in the construction of highways,resulting in an improved highway network that has provided robust support for economic and social development.However,the rapid expansion of highway construction,power supply,and distribution has led to several challenges in mechanical and electrical engineering technology.Ensuring the safe,stable,and cost-effective operation of the power supply and distribution system to meet the diverse requirements of highway operations has become a pressing issue.This article takes an example of a highway electromechanical engineering power supply and distribution construction project to provide insight into the construction process of highway electromechanical engineering power supply and distribution technology.
基金Digital Twin and Acoustic Perception Research Team(2021XJTD06)。
文摘With the rapid development of information technology,the demand for talents in the field of software engineering is growing.In order to cultivate high-quality software engineering talents who meet the market demand,universities have continuously carried out the construction of software engineering majors.Accreditation Board for Engineering and Technology(ABET)certification,as an internationally recognized higher education quality assurance system,provides important reference and guidance for the construction of software engineering majors.Guided by student learning outcomes and core competencies,combined with the characteristics of software engineering talent cultivation,the innovation of talent cultivation mode takes industry-education integration and school-enterprise cooperation as the main development paths and explores comprehensive reform of the major in terms of professional positioning and goals,curriculum system,teaching conditions,and teachers.This comprehensive reform model has effectively promoted the development of major construction and improved the quality of talent cultivation.
文摘To better improve the electrical safety of buildings and fulfill the role of lightning protection and grounding,this article combines cases and discusses seven aspects of lightning protection devices’construction technology.These aspects include the lightning protection classification of buildings,air terminals,down conductors,grounding devices,division of lightning protection zones,and lightning electromagnetic pulse shielding.Through the introduction of this article,readers can gain a comprehensive understanding of the application and value of lightning protection grounding construction technology in building electrical installation projects.
文摘The purpose of this paper is to discuss the integration of the elements of civic and political education into the engineering geology course to improve students’ideological and moral qualities.It is proposed that by integrating elements of civic and political education,students are guided to form a positive attitude toward engineering practice as well as correct values and ethics.With regard to the teaching design and implementation of the course,the implementation paths of teacher team building,careful teaching design,innovative teaching methods,and the integration of civic and politics in practical teaching are proposed to summarize the significance of integrating the elements of civic and political education in the construction of the engineering geology course.It is pointed out that this integration not only improves the quality of the course,but also provides a reference for the civic and political education of other similar professional courses.This integration not only focuses on the teaching of professional knowledge,but also pays more attention to the cultivation of students’ideology and morality,which provides a model and guidance for shaping new talents with all-round development.
基金the Chongqing Engineering College Educational Teaching Reform Research Project“Practical Teaching Research of Road Engineering Construction Technology and Organization Course Based on BIM+Virtual Simulation Technology”(Project No.JY2021310).
文摘We focus on the goal of undergraduate talent training,consider the course features of Road Engineering Construction Technology and Organization,analyze the problems existing in the practical teaching of the course,use the advantages of BIM+virtual simulation technology,design a scientific and reasonable practical teaching content of Road Engineering Construction Technology and Organization,and address the contrast between the strong practical aspect of the traditional Road Engineering Construction Technology and Organization course and the lack of practical instruments in hope to improve students’learning autonomy,enhance the quality of practical teaching,achieve the training objectives of the course,and nurture applied technical talents.
文摘Targeted treatment of cancer with monoclonal antibodies increases the benefit for patients. In order to improve the anti-tumor activity of monoclonal antibodies, multi-specific antibodies have entered the research field. The emergence of various techniques to produce multi-specific recombinant antibody molecules has led to the selection of target combinations in various forms. To date, only a few multi-specific constructs have entered phase III clinical trials, in contrast to classical monoclonal antibodies. Some of the format options are outlined from a technical point of view. We focus on the achievements and prospects of the underlying technologies for generating biand multispecific antibodies.
文摘The design process of the built environment relies on the collaborative effort of all parties involved in the project.During the design phase,owners,end users,and their representatives are expected to make the most critical design and budgetary decisions-shaping the essential traits of the project,hence emerge the need and necessity to create and integrate mechanisms to support the decision-making process.Design decisions should not be based on assumptions,past experiences,or imagination.An example of the numerous problems that are a result of uninformed design decisions is“change orders”,known as the deviation from the original scope of work,which leads to an increase of the overall cost,and changes to the construction schedule of the project.The long-term aim of this inquiry is to understand the user’s behavior,and establish evidence-based control measures,which are actions and processes that can be implemented in practice to decrease the volume and frequency of the occurrence of change orders.The current study developed a foundation for further examination by proposing potential control measures,and testing their efficiency,such as integrating Virtual Reality(VR).The specific aim was to examine the effect of different visualization methods(i.e.,VR vs.construction drawings)on,(1)how well the subjects understand the information presented about the future/planned environment;(2)the subjects’perceived confidence in what the future environment will look like;(3)the likelihood of changing the built environment;(4)design review time;and(5)accuracy in reviewing and understanding the design.
文摘With the introduction of“new engineering,new medical science,new agricultural science,and new liberal arts”construction,the reform of college English teaching is also imperative.Scholars nationwide have conducted attempts in different aspects of college English teaching and produced fruitful results.Yet,more efforts should be made.This paper discusses the reforms made by Wuhan University of Technology,focusing on the reformation of curriculum.
文摘Software engineering is an engineering discipline that guides software developers to carry out software development,software maintenance,and software management.The traditional evaluation methods of software engineering courses do not highlight the training goal of outcomebased education(OBE).This paper systematically studies the evaluation methods for practical courses in software engineering from three aspects:course evaluation guidelines,course evaluation methods and course evaluation effects;establishes a comprehensive,scientific,and objective system of course evaluation;effectively measures students’learning effect;promotes teachers to continuously improve the teaching process;and thus improves the teaching quality of software engineering courses.Besides the general approach,this paper also takes software project construction practice as an example to demonstrate the effect of the proposed approach.
基金supported by the National Natural Science Foundation of China(22162008,22162007)the Science and Technology Supporting Project of Guizhou Province([2022]208,[2021]480)the Basic Research Program of Science&Technology Department of Guizhou Province([2020]1Y055)。
文摘Interface engineering can improve the charge separation efficiency and inhibit photocorrosion is an emerging direction of developing more efficient and cost-effective photocatalytic systems.Herein,we report the sulfur-confined intimate Cd S intergrown Cd(Cd S/Cd)Ohmic junction(peanut-chocolate-ball like)for high-efficient H2production with superior anti-photocorrosion ability,which was fabricated from in-situ photoreduction of CdS intergrown Cd2SO4(OH)2(CdS/Cd2SO4(OH)2)prepared through a facile space-controlled-solvothermal method.The ratios of CdS/Cd can be effectively controlled by tunning that of CdS/Cd2SO4(OH)2which were prepared by adjusting the volume of reaction liquid and the remaining space of the reactor.Experiments investigations and density functional theory(DFT)calculations reveal that the Cd S intergrown Cd Ohmic junction interfaces(with appropriate content Cd intergrown on Cd S(19.54 wt%))are beneficial in facilitating the transfer of photogenerated electrons by constructing an interfacial electric field and forming sulfur-confined structures for preventing the positive holes(h+)oxidize the Cd S.This contributes to a high photocatalytic H2production activity of 95.40μmol h-1(about 32.3 times higher than bare Cd S)and possesses outstanding photocatalytic stability over 205 h,much longer than most Cd S-based photocatalysts previously reported.The interface engineering design inspired by the structure of peanut-chocolate-ball can greatly promote the future development of catalytic systems for wider application.
文摘Mesaconic acid has a special chemical structure and can undergo a series of reactions such as polymerization and addition. It is an important chemical intermediate and widely used in material, chemical and other industries. The chemical synthesis of mesaconic acid requires nitric acid, which is dangerous and harmful to the environment. The production of mesaconic acid by microbial fermentation has the characteristics of low raw material price, high efficiency and strong specificity, and thus a strong industrial application prospect. Mesaconic acid is an intermediate product of glutamic acid degradation pathway of microorganisms such as Clostridium tetani. However, at present, few reports have been conducted on the production of mesaconic acid by metabolic engineering microorganisms. In this study, glutamate mutase(GLM) and 3-methylaspartate ammonialyase(MAL) from C. tetani were recombined and expressed in Escherichia coli, and the obtained strain, BL21(DE3)/pETDuet-1-MAL-mutS-mutE, achieved the yield of mesaconic acid of 1.06 g/L. Compared with the wild type, the yields of mesaconic acid from mutants G133A and G133S increased by 21% and 16%, respectively. After 24 h of flask fermentation, the yields of mesaconic acid reached 1.28 and 1.23 g/L, respectively. This study can provide reference for microbial synthesis of mesaconic acid.
基金Supported by Education and Teaching Reform Research Project of Pingdingshan University (2020-JY05,2021-JY55)Key Scientific Research Project of Colleges and Universities in Henan Province (22B180011,23A550020)+1 种基金Henan Science and Technology Department Project (232102320262)Key Demonstration Courses with Ideological and Political Theories Teaching in Pingdingshan University in 2022.
文摘Environmental Microbiology is a professional basic course in the curriculum system of Environmental and Ecological Engineering major,which follows the requirements of ideological and political theories teaching in the course in higher education,and should be implemented in every course.Therefore,the construction of ideological and political theories teaching in the course should be an important task in the course construction of Environmental Microbiology.In view of the curriculum characteristics of Environmental Microbiology,such as multi-disciplinary feature,wide coverage and rapid development,it is an effective method for the construction of ideological and political theories teaching in the course of Environmental Microbiology by fully tapping the ideological and political elements of the curriculum,such as patriotism,scientific spirit and practical innovation,and integrating the ideological and political theories teaching in the course into the whole curriculum design and classroom teaching construction.
文摘With the development of China's economy,the implementation scale and scope of hydraulic engineering projects are increasing,and the application of anti-seepage technology has an important influence on the construction quality of hydraulic engineering.In this paper,the significance and problems of the application of anti-seepage technology in the construction of hydraulic engineering projects were mainly analyzed,and specific application strategies were proposed.
文摘Municipal civil engineering is the key content of municipal construction,and the construction scale is usually large.The quality of the project plays an important role in the development of urban economy.Due to the rapid increase of high-rise buildings,skyscrapers and underground buildings,the construction technology of deep foundation pit support has gradually become an indispensable construction technology.Therefore,the selection of foundation pit support construction technology is crucial in ensuring that whether the foundation is firm and stable,and whether the subsequent construction activities can be carried out smoothly.In view of this,the article discusses the application of deep foundation pit support construction technology in municipal civil engineering,aiming to provide reference for subsequent projects.
