Background:Type 2 diabetes(T2D)is a polygenic metabolic disease,character-ized by high fasting blood glucose(FBG).The ability of cranberry(CRN)fruit to regulate glycemia in T2D patients is well known.Here,a cohort of ...Background:Type 2 diabetes(T2D)is a polygenic metabolic disease,character-ized by high fasting blood glucose(FBG).The ability of cranberry(CRN)fruit to regulate glycemia in T2D patients is well known.Here,a cohort of 13 lines of the genetically diverse Collaborative Cross(CC)mouse model was assessed for the effect of non-dialyzable material(NDM)of cranberry extract in lowering fasting blood glucose.Methods:Eight-week-old mice were maintained on either a standard chow diet(con-trol group)or a high-fat diet(HFD)for 12 weeks,followed by injections of intraperi-toneal(IP)NDM(50 mg/kg)per mouse,three times a week for the next 6 weeks.Absolute FBG(mg/dl)was measured bi-weekly and percentage changes in FBG(%FBG)between weeks 0 and 12 were calculated.Results:Statistical analysis showed a significant decrease in FBG between weeks 0 and 12 in male and female mice maintained on CHD.However,a non-significant in-crease in FBG values was observed in male and female mice maintained on HFD dur-ing the same period.Following administration of NDM during the following 6 weeks,the results show a variation in significant levels of FBG lowering between lines,male and female mice and under the different diets.Conclusion:The results suggest that the efficacy of NDM treatment in lowering FGB depends on host genetic background(pharmacogenetics),sex of the mouse(phar-macosex),and diet(pharmacodiet).All these results support the need for follow-up research to better understand and implement a personalized medicine approach/uti-lization of NDM for reducing FBG.展开更多
AIM： To study isolation and chemical characterization of pectin derived from the common cranberry Vaccin/um oxycoccos L. （oxycoccusan OP） and the testing of its preventive effect on experimental colitis. METHODS： ...AIM： To study isolation and chemical characterization of pectin derived from the common cranberry Vaccin/um oxycoccos L. （oxycoccusan OP） and the testing of its preventive effect on experimental colitis. METHODS： Mice were administrated orally with OP two days prior to a rectal injection of 5% acetic acid and examined for colonic damage 24 h later. Colonic inflammation was characterized by macroscopical injury and enhanced levels of myeloperoxidase activity measured spectrophotometrically with o-phenylene diamine as the substrate. The mucus contents of the colon were determined by the Alcian blue dye binding method. Vascular permeability was estimated using 4% Evans blue passage after i.p. injection of 0.05 mol/L acetic acid. RESULTS： In the mice treated with OP, colonic macroscopic scores （1.1 ± 0.4 vs 2.7, P 〈 0.01） and the total square area of damage （10 ± 2 vs 21 ± 7, P 〈 0.01） were significantly reduced when compared with the vehicle-treated colitis group. OP was shown to decrease the tissue myeloperoxidase activity in colons （42 ± 11 vs 112 ± 40, P 〈 0.01） and enhance the amount of mucus of colitis mice （0.9 ± 0.1 vs 0.4 ± 0.1, P 〈 0.01）. The level of colonic malondialdehyde was noted to decrease in OP-pretreated mice （3.6 ± 0.7 vs 5.1 ± 0.8, P 〈 0.01）. OP was found to decrease the inflammatory status of mice as was determined by reduction of vascular permeability （161 ± 34 vs 241 ± 21, P 〈 0.01）. Adhesion of peritoneal neutrophils and macrophages was also shown to decrease after administration of OP （141 ± 50 vs 235 ± 37, P 〈 0.05）. CONCLUSION： Thus, a preventive effect of pectin from the common cranberry, namely oxycoccusan OP, on acetic acid-induced colitis in mice was detected. A reduction of neutrophil infiltration and antioxidant action may be implicated in the protective effect of oxycoccusan.展开更多
ObjectiveTo investigate the hepatoprotective efficacy of cranberry extract (CBE) against carbon tetrachloride (CCl<sub>4</sub>)-induced hepatic injury using in-vivo animal model.MethodsThe hepatoprotective...ObjectiveTo investigate the hepatoprotective efficacy of cranberry extract (CBE) against carbon tetrachloride (CCl<sub>4</sub>)-induced hepatic injury using in-vivo animal model.MethodsThe hepatoprotective efficacy of CBE (200 and 400 mg/kg) was investigated against CCl<sub>4</sub> (4 mL/kg)-induced hepatotoxicity, elevated liver enzymes [ALT (alanine aminotransferase), AST (aspartate aminotransferase), and alkaline phosphatase (ALP)], and total protein (TP) contents in the serum. Moreover, CBE-aided antioxidant defense against hepatotoxic insult of CCl<sub>4</sub> was measured by evaluating a number of anti-oxidative biomarkers including reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in the serum by using spectrophotometric analyses.ResultsResults showed that the exposure of experimental animals to CCl<sub>4</sub> did induce significant hepatotoxicity compared to the non-induced (untreated) group. The oral administration of CBE demonstrated a significant dose-dependent alleviation in the liver enzymes (AST, ALT, and ALP), increased antioxidant defense (GSH, SOD, and CAT), and reduced MDA levels in the serum of treated animals compared to the animals without treatment. The resulting data showed that the administration of CBE decreased the serum levels of ALT, AST, and ALP compared to the CCl<sub>4</sub>-induced group.ConclusionsThe resulting data evidenced that CBE exhibits promising hepatoprotective potential against the chemical induced hepatotoxicity, maintains homeostasis in liver enzymes, and can provide significant antioxidant defense against free radicals-induced oxidative stress.展开更多
Common bean(Phaseolus vulgaris) is an annual legume crop that is grown worldwide for its edible dry seeds and tender pods. Marsh spot(MS) of the seeds is a physio-genic stress disease affecting seed quality in beans. ...Common bean(Phaseolus vulgaris) is an annual legume crop that is grown worldwide for its edible dry seeds and tender pods. Marsh spot(MS) of the seeds is a physio-genic stress disease affecting seed quality in beans. Studies have suggested that this disease involves a nutritional disorder caused by manganese deficiency, but the inheritance of resistance to this disease has not been reported. A biparental genetic population composed of 138 recombinant inbred lines(RILs) was developed from a cross between an MS resistant cultivar ‘Cran09’ and an MS susceptible cultivar ‘Messina’. The 138 RILs and their two parents were evaluated for MS resistance during five consecutive years from 2015 to 2019 in sandy and heavy clay soils in Morden, Manitoba, Canada. The MS incidence(MSI) and the MS resistance index(MSRI) representing disease severity were shown to be both highly correlated heritable traits that had high broad-sense heritability values(H;) of 86.5% and 83.2%, respectively. No significant differences for MSI and MSRI were observed between the two soil types in all five-(MSI) or four-year(MSRI) data collection, but significant correlations among years were observed despite MS resistance was moderately affected by year. The MSIs and MSRIs displayed a right-skewed distribution, indicating a mixed genetic model involving a few major genes and polygenes. Using the joint segregation analysis method, the same four major genes with additive-epistasis effects showed the best fit for both traits, explaining 84.4% and85.3% of the phenotypic variance for MSI and MSRI, respectively. For both traits, the M1, M2, M3 and m4 acted as the favorable(resistant) alleles for the four genes where M and m represent two alleles of each gene. However, due to epistatic effects, only the individuals of the M1 M2 M3 M4 haplotype appeared to be highly resistant, whereas those of the m1 m2 m3 M4 haplotype were the most susceptible. The m4 allele significantly suppressed the additive effects of M1 M2 M3 on resistance, but decreased susceptibility due to the additive effects of m1 m2 m3. Further quantitative trait locus(QTL) mapping is warranted to identify and validate individual genes and develop molecular markers for marker-assisted selection of resistant cultivars.展开更多
Frost can cause serious economic losses in cranberry fields, particularly in northern regions. When the air temperature reaches a low critical threshold, sprinklers are operated to protect vines, to insure crop produc...Frost can cause serious economic losses in cranberry fields, particularly in northern regions. When the air temperature reaches a low critical threshold, sprinklers are operated to protect vines, to insure crop production and profitability. To avoid frost injury, proper positioning of temperature sensors is critical. A field experiment was designed and conducted to determine the optimal installation height of sensors above soil surface. Temperature data was used to investigate the spatial temperature gradient in the section of a cranberry field. A computer simulation of the temperature profile was performed to simulate the effect of wind velocity on the prediction of air temperature. For optimal use, sensors should be installed at the height of the canopy and several meters away from a dike. On nights with low wind velocities, the canopy air temperature was 2.7°C below that of 500 cm above the ground. The sensors should be put at least five m away from a dike to avoid the transfer of heat from the dike to the sensor. Also, multiple sensors should be installed because of the large variations in air temperature that were measured across the experiment. The simulated temperature indicated that wind velocity strongly influenced the temperature estimation;the effect of the wind on temperatures gradients was greater when the wind velocity was low (<2.3 m/s).展开更多
Field studies were conducted in 2009 and 2010 at the Huron Research Station, Exeter, Ontario and the University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the tolerance of four cultivars of cranberry ...Field studies were conducted in 2009 and 2010 at the Huron Research Station, Exeter, Ontario and the University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the tolerance of four cultivars of cranberry bean (“Etna”, “Hooter”, “SVM Taylor”, and “Capri”) and four cultivars of kidney bean (“Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty”) to linuron applied preemergence at 1125 and 2250 g·ai·ha-1. One week after emergence (WAE), linuron applied PRE caused 0.4% to 1.2% injury in “Etna”, “Hooter”, “SVM Tayler”, and “Capri” cranberry bean and 3.1% to 3.6% injury in “Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty” kidney bean. At 2 and 4 WAE, there was no difference in injury among the dry bean cultivars. Contrast comparing injury due to linuron in cranberry vs kidney bean cultivars indicated 2.3%, 1.7%, and 1.2% greater injury in kidney bean compared to cranberry bean at 1, 2, and 4 WAE, respectively. Linuron PRE caused slightly greater injury in kidney bean compared to cranberry bean but crop injury was minimal with no adverse effect on plant height, shoot dry weight, seed moisture content, and yield under the environments evaluated. Based on this research, linuron applied PRE at the proposed rate of 1125 g·ai·ha-1 can be safely used in cranberry and kidney beans in Ontario.展开更多
This work aimed to find the effect of cranberry extract (75 and 150 mg/kg·b·w) and vit. C (1 g/kg·b·w orally) on renal toxicity induced by Diclofenac sodium in male albino r...This work aimed to find the effect of cranberry extract (75 and 150 mg/kg·b·w) and vit. C (1 g/kg·b·w orally) on renal toxicity induced by Diclofenac sodium in male albino rats. Treated rats with diclofenac sodium with a concentration 150 mg/kg·b·w, expressed a significant increase in several parameters includes, plasma total cholesterol, LDL-cholesterol, and triglyceride as well as renal nitric oxide (NO), tumor necrosis factor-alfa (TNF-α) and TBARS. In addition, a significant reduction in renal superoxide dismutase (SOD), GSH, catalase (CAT) and plasma HDL. The present results explain that, using cranberry extract and vit. C resulted in increasing the level of GSH, CAT and SOD as well as gene expression of renal SOD, CAT and IL-22 and reduce the level of TBARs significantly which led to preventing renal tissue damage. Our results also revealed that cranberry extract can protect DNA from damage as obtained from comet essay. TM-U was elevated in DCLF treated group when compared with normal. However cranberry extract was able to reduce this elevation in dose dependant manner. Histological features in H&E taken to different groups also mirrors this findings. DCLF causes many changes in renal tissue include infiltration by inflammatory cells, attenuated glomeruli, apoptosis in tubular epithelia.展开更多
Cranberry,the fruit of Vaccinium macrocarpon Aiton,is becoming popular all over the world,due to its pleasant taste,nutraceutical value and biological activities.For a long time,98%of its yield has been from America.S...Cranberry,the fruit of Vaccinium macrocarpon Aiton,is becoming popular all over the world,due to its pleasant taste,nutraceutical value and biological activities.For a long time,98%of its yield has been from America.Since being introduced to China as an import in recent decades,cranberry has become a rapid success in the food industry even with the high transportation cost.Recently,V.macrocarpon was cultivated in the Northeast of China,and the area of cultivation has increased dramatically.Therefore,it is important to systematically determine the useful compounds and their activity in this“Made-in-China”cranberry.In the present study,the content of total phenolics,anthocyanins,proanthocyanidins,sugars and organic acids was determined.Our results demonstrated that domestic cranberry is a rich source of phenolics,anthocyanins,proanthocyanidins,sugars and organic acids.Analysis of the chemical composition of an acidic ethanol extract by UPLC-QTOF-MS identified 21 compounds as well as the contents determination of catechin and cyanidin-3-O-glucoside using RPHPLC.Furthermore,cranberry extract was tested for its anti-bacterial activity against five clinically important pathogens,as well as its inhibitory effect on the proliferation and migration of cancer cells in vitro.Our results demonstrated that cranberry produced in Chinese cultivation areas is a rich source of bioactive compounds and exhibited in vitro anti-microbial and anti-tumor activities.Our work provides essential information about the quality of domestic cranberry and facilitates the development of related industries.展开更多
Lipid peroxidation inhibition capacity and antiradical activity were evaluated in HPLC fractions of different polarity obtained from two cranberry juices and three extracts isolated from frozen cranberries and pomace ...Lipid peroxidation inhibition capacity and antiradical activity were evaluated in HPLC fractions of different polarity obtained from two cranberry juices and three extracts isolated from frozen cranberries and pomace containing antho-cyanins, water-soluble and apolar phenolic compounds, respectively. Compounds with close polarities were collected to obtain between three and four fractions from each juice or extract. The cranberry phenols are good free radi-cal-scavengers, but they were less efficient at inhibiting the lipid peroxidation. Of all the samples tested, the intermediate polarity fraction of extract rich in apolar phenolic compounds of fruit presented the highest antiradical activity while the most hydrophobic fractions of the anthocyanin-rich extract from fruit and pomace appeared to be the most efficient at inhibiting the lipid peroxidation. The antioxidant or pro-oxidant activity of fractions increased with the con-centration. The phenol polarity and the technological process to manufacture cranberry juice can influence the antioxidant and antiradical activities of fractions.展开更多
Cranberry extract(CBE) rich in polyphenols are potent to delay paralysis induced by alleviating β-amyloid(Aβ) toxicity in C. elegans model of Alzheimer's disease(AD). In order to better apply CBE as an anti-AD a...Cranberry extract(CBE) rich in polyphenols are potent to delay paralysis induced by alleviating β-amyloid(Aβ) toxicity in C. elegans model of Alzheimer's disease(AD). In order to better apply CBE as an anti-AD agent efficiently, we sought to deterrmine whether preventive or therapeutic effect contributes more prominently toward CBE's anti-AD activity. As the level of Aβtoxicity and memory health are two major pathological parameters in AD, in the present study, we compared the effects of CBE on Aβtoxicity and memory health in the C. elegans AD model treated with preventive and therapeutic protocols. Our results revealed that CBE prominently showed the preventive efficacy, providing a basis for further investigation of these effects in mammals.展开更多
Culturable fungal population diversity and succession was investigated in developing cranberry ovaries of fruit rot-resistant and rot-susceptible cranberry selections,from flower through mature fruit.Fungi were recove...Culturable fungal population diversity and succession was investigated in developing cranberry ovaries of fruit rot-resistant and rot-susceptible cranberry selections,from flower through mature fruit.Fungi were recovered in culture from 1185 of 1338 ovary tissues collected from June to September,yielding 2255 isolates that represented 42 morphotaxa.During the season,species richness varied from 2 to 17 and 2 to 18 in rot-resistant and rotsusceptible selections,respectively,increasing from wk1 to wk10 and then gradually declining to wk14.Shannon-Wiener diversity index varied from 0.27 to 2.32 in rotresistant and 0.18 to 2.38 in rot-susceptible,and Pielou’s evenness index varied from 0.11 to 0.63 and 0.06 to 0.64 in rot-resistant and rot-susceptible selections,respectively,confirming that diversity of fungi in developing ovaries was similar among rot-resistant and rot-susceptible selections,but varied among sampling time points.Principal component analysis grouped samples collected at the same sampling time point together regardless of rot susceptibility of the selections,and detected the predominant fungal species associated with each stage of development.Successional changes were observed in populations of endophytic,pathogenic and saprophytic fungi throughout the season as ovaries matured.展开更多
Fuzzy chromatography mass spectrometric(FCMS)fingerprinting methods in combination with principal component analysis(PCA)and soft independent modeling by class analogy(SIMCA)were developed for phenolic profiling and d...Fuzzy chromatography mass spectrometric(FCMS)fingerprinting methods in combination with principal component analysis(PCA)and soft independent modeling by class analogy(SIMCA)were developed for phenolic profiling and differentiation of American cranberry cultivars.Two FCMS fingerprinting methods,ion mobility fuzzy chromatography mass spectrometric(imFCMS)and conventional FCMS,are compared in the study.PCA and SIMCA successfully differentiated the cultivars with both methods.The six cultivars used formed three distinct groups in the PCA score plots,and each group contained one wild selection cultivar and its genetically related hybrid(s).Compared with FCMS fingerprinting,imFCMS fingerprinting provided better intra-cultivar sample clustering and inter-cultivar sample separation in PCA and superior sample classifica-tion sensitivity in SIMCA.Ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-HRMS)analysis was conducted on selected samples to profile the phenolic difference between the cultivars.Compound identification using UPLC-HRMS revealed that flavonoid compounds,including flavonol glycosides,proanthocyanidins and anthocyanins,are major components that contribute to the phenolic profile variation among cultivars.展开更多
Many food and plant extracts have shown in vitro antiHelicobacter pylori(H.pylori)activity,but are less effective in vivo.The anti-H.pylori effects of these extracts are mainly permeabilitization of the membrane,anti-...Many food and plant extracts have shown in vitro antiHelicobacter pylori(H.pylori)activity,but are less effective in vivo.The anti-H.pylori effects of these extracts are mainly permeabilitization of the membrane,anti-adhesion,inhibition of bacterial enzymes andbacterial grown.We,herein,review treatment effects of cranberry,garlic,curcumin,ginger and pistacia gum against H.pylori in both in vitro,animal studies and in vivo studies.展开更多
Objective:Despite conflicting evidence,it is common practice to use continuous antibiotic prophylaxis(CAP)in patients with indwelling double-J(DJ)stents.Cranberry extracts and d-mannose have been shown to prevent colo...Objective:Despite conflicting evidence,it is common practice to use continuous antibiotic prophylaxis(CAP)in patients with indwelling double-J(DJ)stents.Cranberry extracts and d-mannose have been shown to prevent colonization of the urinary tract.We evaluated their role in this setting.Methods:We conducted a prospective randomized study to evaluate patients with indwelling DJ stents following urological procedures.They were randomized into three groups.Group A(n=46)received CAP(nitrofurantoin 100 mg once daily[OD]).Group B(n=48)received cranberry extract 300 mg and d-mannose 600 mg twice daily(BD).Group C(n=40)received no prophylaxis.The stents were removed between 15 days and 45 days after surgery.Three groups were compared in terms of colonization of stent and urine,stent related symptoms and febrile urinary tract infections(UTIs)during the period of indwelling stent and until 1 week after removal.Results:In Group A,9(19.5%)patients had significant bacterial growth on the stents.This was 8(16.7%)in the Group B and 5(12.5%)in Group C(p-value:0.743).However,the culture positivity rate of urine specimens showed a significant difference(p-value:0.023)with Group B showing least colonization of urine compared to groups A and C.There was no statistically significant difference in the frequency of stent related symptoms(p-value:0.242)or febrile UTIs(p-value:0.399)among the groups.Conclusion:Prophylactic agents have no role in altering bacterial growth on temporary indwelling DJ stent,stent related symptoms or febrile UTIs.Cranberry extract may reduce the colonization of urinary tract,but its clinical significance needs further evaluation.展开更多
There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cra...There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cranberry, kidney, otebo, pink, pinto, Small Red Mexican and white bean to the pendimethalin applied preplant incorporated at 1080 and 2160 g.a.i.ha-1. Pendimethalin PPI caused minimal injury in most market classes of dry bean at 1 and 2 WAE. There was no injury in various market classes of dry bean with the low dose at 1 and 2 weeks after emergence (WAE). However, at the high dose there was 0 to 4% injury at 1 WAE and 0 to 7% injury at 2 WAE in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Pendimethalin PPI was more injurious in white bean than in black, cranberry, kidney, otebo, pink, pinto and SRM bean. Pink and SRM bean exhibited the most tolerance to pendimethalin applied PPI at 1080 g.ai.ha-1 or 2160 g.ai.ha-1. Pendimethalin caused no adverse effect on plant height, shoot dry weight, seed moisture content and seed yield of black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Based on these results, there is an adequate margin of crop safety for pendimethalin applied PPI at the proposed dose of 1080 g.ai.ha-1 in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean in Ontario.展开更多
Eight field trials were conducted from 2006 to 2008 at various locations in Ontario to evaluate the co-application of postemergence herbicides with cyhalothrin-lambda or dimethoate insecticides in cranberry and white ...Eight field trials were conducted from 2006 to 2008 at various locations in Ontario to evaluate the co-application of postemergence herbicides with cyhalothrin-lambda or dimethoate insecticides in cranberry and white bean. At 2 weeks after treatment, the addition of cyhalothrin-lambda or dimethoate insecticides to sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen did not increase injury at the Exeter and Ridgetown locations except for bentazon plus dimethoate which caused greater injury than bentazon alone (2.9% vs 0.2%) in 2006. However at Harrow, the addition of dimethoate to quizalofop-p-ethyl increased injury (0% vs 4.9%) in 2007 and the addition cyhalothrin-lambda or dimethoate to sethoxydim increased injury in 2008 in dry bean. The addition of cyhalothrin-lambda to quizalofop-p-ethyl also increased injury (0% vs 4.5%) in 2008. There was no adverse effect on dry bean injury with other treatments at Harrow in 2007 or 2008. The addition of cyhalothrin-lambda or dimethoate to the herbicides evaluated did not have any adverse effect on plant height, shoot dry weight or yield of dry bean except for bentazon plus dimethoate which decreased shoot dry weight 20% compared to bentazon alone at Harrow in 2008. Based on these results, cyhalothrin-lambda or dimethoate can be tank-mixed with sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen when the optimum application timing of these herbicides and insecticides coincide.展开更多
The effect of biostimulants such as Crop Booster and RR SoyBooster on dry bean under Ontario environmental conditions is not known. A total of 12 field experiments (6 in cranberry bean “Etna” and 6 in white bean “O...The effect of biostimulants such as Crop Booster and RR SoyBooster on dry bean under Ontario environmental conditions is not known. A total of 12 field experiments (6 in cranberry bean “Etna” and 6 in white bean “OAC REX”) were conducted at two locations (Ridgetown and Exeter, Ontario, Canada) to evaluate the effect of Crop Booster and RR SoyBooster on visible injury, shoot dry weight, height and yield of cranberry and white bean. Visible injury ranged from 0% to 7.3% in white bean and 0% to 9.4% in cranberry bean with quizalofop-p-ethyl, bentazon, fomesafen, bentazon plus fomesafen, imazethapyr and imazethapyr plus bentazon alone or in combination with Crop Booster and RR SoyBooster. The addition of Crop Booster or RR SoyBooster to herbicides evaluated had no significant effect on shoot dry weight, height, seed moisture content and yield of white or cranberry bean except with the addition of RR SoyBooster to quizalofop-p-ethyl which increased height 3.7% and the addition of the Crop Booster to bentazon which decreased shoot dry weight 12% and the addition of Crop Booster to bentazon plus fomesafen which increased shoot dry weight 17% in white bean. Based on these results, there were minimal effects from the addition of Crop Booster or RR SoyBooster to commonly used herbicides in white and cranberry bean.展开更多
This review provides an examination of the marsh spot disease in beans and the roles played by its causal factor, manganese (Mn) deficiency. The discovery of the marsh spot disease, its relation with Mn deficiency, an...This review provides an examination of the marsh spot disease in beans and the roles played by its causal factor, manganese (Mn) deficiency. The discovery of the marsh spot disease, its relation with Mn deficiency, and how it can be treated are discussed. Mn serves as a cofactor and a catalyst in various metabolic processes in different cell compartments, such as the oxygen-evolving complex of photosystem II (PSII) or reactive oxygen species scavenging. Some major quantitative trait loci (QTL) and putative candidate genes associated with Mn content in plants, especially in plant seeds, have been identified. Marsh spot disease in cranberry common bean is controlled by several major genes with significant additive and epistatic effects. They provide valuable clues for QTL candidate gene prediction and an improved understanding of the genetic mechanisms responsible for marsh spot resistance in plants.展开更多
基金supported by a core fund from Tel-Aviv University.
文摘Background:Type 2 diabetes(T2D)is a polygenic metabolic disease,character-ized by high fasting blood glucose(FBG).The ability of cranberry(CRN)fruit to regulate glycemia in T2D patients is well known.Here,a cohort of 13 lines of the genetically diverse Collaborative Cross(CC)mouse model was assessed for the effect of non-dialyzable material(NDM)of cranberry extract in lowering fasting blood glucose.Methods:Eight-week-old mice were maintained on either a standard chow diet(con-trol group)or a high-fat diet(HFD)for 12 weeks,followed by injections of intraperi-toneal(IP)NDM(50 mg/kg)per mouse,three times a week for the next 6 weeks.Absolute FBG(mg/dl)was measured bi-weekly and percentage changes in FBG(%FBG)between weeks 0 and 12 were calculated.Results:Statistical analysis showed a significant decrease in FBG between weeks 0 and 12 in male and female mice maintained on CHD.However,a non-significant in-crease in FBG values was observed in male and female mice maintained on HFD dur-ing the same period.Following administration of NDM during the following 6 weeks,the results show a variation in significant levels of FBG lowering between lines,male and female mice and under the different diets.Conclusion:The results suggest that the efficacy of NDM treatment in lowering FGB depends on host genetic background(pharmacogenetics),sex of the mouse(phar-macosex),and diet(pharmacodiet).All these results support the need for follow-up research to better understand and implement a personalized medicine approach/uti-lization of NDM for reducing FBG.
基金the Program of Presidium of the Russian Acad.Sci. <>, the Russian Fund forBasic Research, No. 06-04-48079 the Program for LeadingScientific Schools, No. 5796.2006.4
文摘AIM： To study isolation and chemical characterization of pectin derived from the common cranberry Vaccin/um oxycoccos L. （oxycoccusan OP） and the testing of its preventive effect on experimental colitis. METHODS： Mice were administrated orally with OP two days prior to a rectal injection of 5% acetic acid and examined for colonic damage 24 h later. Colonic inflammation was characterized by macroscopical injury and enhanced levels of myeloperoxidase activity measured spectrophotometrically with o-phenylene diamine as the substrate. The mucus contents of the colon were determined by the Alcian blue dye binding method. Vascular permeability was estimated using 4% Evans blue passage after i.p. injection of 0.05 mol/L acetic acid. RESULTS： In the mice treated with OP, colonic macroscopic scores （1.1 ± 0.4 vs 2.7, P 〈 0.01） and the total square area of damage （10 ± 2 vs 21 ± 7, P 〈 0.01） were significantly reduced when compared with the vehicle-treated colitis group. OP was shown to decrease the tissue myeloperoxidase activity in colons （42 ± 11 vs 112 ± 40, P 〈 0.01） and enhance the amount of mucus of colitis mice （0.9 ± 0.1 vs 0.4 ± 0.1, P 〈 0.01）. The level of colonic malondialdehyde was noted to decrease in OP-pretreated mice （3.6 ± 0.7 vs 5.1 ± 0.8, P 〈 0.01）. OP was found to decrease the inflammatory status of mice as was determined by reduction of vascular permeability （161 ± 34 vs 241 ± 21, P 〈 0.01）. Adhesion of peritoneal neutrophils and macrophages was also shown to decrease after administration of OP （141 ± 50 vs 235 ± 37, P 〈 0.05）. CONCLUSION： Thus, a preventive effect of pectin from the common cranberry, namely oxycoccusan OP, on acetic acid-induced colitis in mice was detected. A reduction of neutrophil infiltration and antioxidant action may be implicated in the protective effect of oxycoccusan.
基金the Institute of Molecular Biology and Biotechnology (IMBB), University of Lahore for providing resources and financial support
文摘ObjectiveTo investigate the hepatoprotective efficacy of cranberry extract (CBE) against carbon tetrachloride (CCl<sub>4</sub>)-induced hepatic injury using in-vivo animal model.MethodsThe hepatoprotective efficacy of CBE (200 and 400 mg/kg) was investigated against CCl<sub>4</sub> (4 mL/kg)-induced hepatotoxicity, elevated liver enzymes [ALT (alanine aminotransferase), AST (aspartate aminotransferase), and alkaline phosphatase (ALP)], and total protein (TP) contents in the serum. Moreover, CBE-aided antioxidant defense against hepatotoxic insult of CCl<sub>4</sub> was measured by evaluating a number of anti-oxidative biomarkers including reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in the serum by using spectrophotometric analyses.ResultsResults showed that the exposure of experimental animals to CCl<sub>4</sub> did induce significant hepatotoxicity compared to the non-induced (untreated) group. The oral administration of CBE demonstrated a significant dose-dependent alleviation in the liver enzymes (AST, ALT, and ALP), increased antioxidant defense (GSH, SOD, and CAT), and reduced MDA levels in the serum of treated animals compared to the animals without treatment. The resulting data showed that the administration of CBE decreased the serum levels of ALT, AST, and ALP compared to the CCl<sub>4</sub>-induced group.ConclusionsThe resulting data evidenced that CBE exhibits promising hepatoprotective potential against the chemical induced hepatotoxicity, maintains homeostasis in liver enzymes, and can provide significant antioxidant defense against free radicals-induced oxidative stress.
基金financial support provided by the Manitoba Pulse and Soybean Growers,AAFC,the Canadian Agricultural Partnership Pulse Science Cluster,and NSERC(RGPIN/2018-03878)。
文摘Common bean(Phaseolus vulgaris) is an annual legume crop that is grown worldwide for its edible dry seeds and tender pods. Marsh spot(MS) of the seeds is a physio-genic stress disease affecting seed quality in beans. Studies have suggested that this disease involves a nutritional disorder caused by manganese deficiency, but the inheritance of resistance to this disease has not been reported. A biparental genetic population composed of 138 recombinant inbred lines(RILs) was developed from a cross between an MS resistant cultivar ‘Cran09’ and an MS susceptible cultivar ‘Messina’. The 138 RILs and their two parents were evaluated for MS resistance during five consecutive years from 2015 to 2019 in sandy and heavy clay soils in Morden, Manitoba, Canada. The MS incidence(MSI) and the MS resistance index(MSRI) representing disease severity were shown to be both highly correlated heritable traits that had high broad-sense heritability values(H;) of 86.5% and 83.2%, respectively. No significant differences for MSI and MSRI were observed between the two soil types in all five-(MSI) or four-year(MSRI) data collection, but significant correlations among years were observed despite MS resistance was moderately affected by year. The MSIs and MSRIs displayed a right-skewed distribution, indicating a mixed genetic model involving a few major genes and polygenes. Using the joint segregation analysis method, the same four major genes with additive-epistasis effects showed the best fit for both traits, explaining 84.4% and85.3% of the phenotypic variance for MSI and MSRI, respectively. For both traits, the M1, M2, M3 and m4 acted as the favorable(resistant) alleles for the four genes where M and m represent two alleles of each gene. However, due to epistatic effects, only the individuals of the M1 M2 M3 M4 haplotype appeared to be highly resistant, whereas those of the m1 m2 m3 M4 haplotype were the most susceptible. The m4 allele significantly suppressed the additive effects of M1 M2 M3 on resistance, but decreased susceptibility due to the additive effects of m1 m2 m3. Further quantitative trait locus(QTL) mapping is warranted to identify and validate individual genes and develop molecular markers for marker-assisted selection of resistant cultivars.
文摘Frost can cause serious economic losses in cranberry fields, particularly in northern regions. When the air temperature reaches a low critical threshold, sprinklers are operated to protect vines, to insure crop production and profitability. To avoid frost injury, proper positioning of temperature sensors is critical. A field experiment was designed and conducted to determine the optimal installation height of sensors above soil surface. Temperature data was used to investigate the spatial temperature gradient in the section of a cranberry field. A computer simulation of the temperature profile was performed to simulate the effect of wind velocity on the prediction of air temperature. For optimal use, sensors should be installed at the height of the canopy and several meters away from a dike. On nights with low wind velocities, the canopy air temperature was 2.7°C below that of 500 cm above the ground. The sensors should be put at least five m away from a dike to avoid the transfer of heat from the dike to the sensor. Also, multiple sensors should be installed because of the large variations in air temperature that were measured across the experiment. The simulated temperature indicated that wind velocity strongly influenced the temperature estimation;the effect of the wind on temperatures gradients was greater when the wind velocity was low (<2.3 m/s).
文摘Field studies were conducted in 2009 and 2010 at the Huron Research Station, Exeter, Ontario and the University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the tolerance of four cultivars of cranberry bean (“Etna”, “Hooter”, “SVM Taylor”, and “Capri”) and four cultivars of kidney bean (“Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty”) to linuron applied preemergence at 1125 and 2250 g·ai·ha-1. One week after emergence (WAE), linuron applied PRE caused 0.4% to 1.2% injury in “Etna”, “Hooter”, “SVM Tayler”, and “Capri” cranberry bean and 3.1% to 3.6% injury in “Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty” kidney bean. At 2 and 4 WAE, there was no difference in injury among the dry bean cultivars. Contrast comparing injury due to linuron in cranberry vs kidney bean cultivars indicated 2.3%, 1.7%, and 1.2% greater injury in kidney bean compared to cranberry bean at 1, 2, and 4 WAE, respectively. Linuron PRE caused slightly greater injury in kidney bean compared to cranberry bean but crop injury was minimal with no adverse effect on plant height, shoot dry weight, seed moisture content, and yield under the environments evaluated. Based on this research, linuron applied PRE at the proposed rate of 1125 g·ai·ha-1 can be safely used in cranberry and kidney beans in Ontario.
文摘This work aimed to find the effect of cranberry extract (75 and 150 mg/kg·b·w) and vit. C (1 g/kg·b·w orally) on renal toxicity induced by Diclofenac sodium in male albino rats. Treated rats with diclofenac sodium with a concentration 150 mg/kg·b·w, expressed a significant increase in several parameters includes, plasma total cholesterol, LDL-cholesterol, and triglyceride as well as renal nitric oxide (NO), tumor necrosis factor-alfa (TNF-α) and TBARS. In addition, a significant reduction in renal superoxide dismutase (SOD), GSH, catalase (CAT) and plasma HDL. The present results explain that, using cranberry extract and vit. C resulted in increasing the level of GSH, CAT and SOD as well as gene expression of renal SOD, CAT and IL-22 and reduce the level of TBARs significantly which led to preventing renal tissue damage. Our results also revealed that cranberry extract can protect DNA from damage as obtained from comet essay. TM-U was elevated in DCLF treated group when compared with normal. However cranberry extract was able to reduce this elevation in dose dependant manner. Histological features in H&E taken to different groups also mirrors this findings. DCLF causes many changes in renal tissue include infiltration by inflammatory cells, attenuated glomeruli, apoptosis in tubular epithelia.
基金This study was financially supported by“Liaoning Provincial Engineering Platform of the Development and Utilization of Endangered and Geo-authentic Medicinal Herbs”and“Propagation of Cranberry and the Establishment of Germplasm Resource Nursery(YZ2018ZR01)”.
文摘Cranberry,the fruit of Vaccinium macrocarpon Aiton,is becoming popular all over the world,due to its pleasant taste,nutraceutical value and biological activities.For a long time,98%of its yield has been from America.Since being introduced to China as an import in recent decades,cranberry has become a rapid success in the food industry even with the high transportation cost.Recently,V.macrocarpon was cultivated in the Northeast of China,and the area of cultivation has increased dramatically.Therefore,it is important to systematically determine the useful compounds and their activity in this“Made-in-China”cranberry.In the present study,the content of total phenolics,anthocyanins,proanthocyanidins,sugars and organic acids was determined.Our results demonstrated that domestic cranberry is a rich source of phenolics,anthocyanins,proanthocyanidins,sugars and organic acids.Analysis of the chemical composition of an acidic ethanol extract by UPLC-QTOF-MS identified 21 compounds as well as the contents determination of catechin and cyanidin-3-O-glucoside using RPHPLC.Furthermore,cranberry extract was tested for its anti-bacterial activity against five clinically important pathogens,as well as its inhibitory effect on the proliferation and migration of cancer cells in vitro.Our results demonstrated that cranberry produced in Chinese cultivation areas is a rich source of bioactive compounds and exhibited in vitro anti-microbial and anti-tumor activities.Our work provides essential information about the quality of domestic cranberry and facilitates the development of related industries.
基金This research was supported by the Natural Sciences and Engineering Research Council of Canada(NSERC) by Atoka Cranberries Inc.(Manseau,QC,Canada).
文摘Lipid peroxidation inhibition capacity and antiradical activity were evaluated in HPLC fractions of different polarity obtained from two cranberry juices and three extracts isolated from frozen cranberries and pomace containing antho-cyanins, water-soluble and apolar phenolic compounds, respectively. Compounds with close polarities were collected to obtain between three and four fractions from each juice or extract. The cranberry phenols are good free radi-cal-scavengers, but they were less efficient at inhibiting the lipid peroxidation. Of all the samples tested, the intermediate polarity fraction of extract rich in apolar phenolic compounds of fruit presented the highest antiradical activity while the most hydrophobic fractions of the anthocyanin-rich extract from fruit and pomace appeared to be the most efficient at inhibiting the lipid peroxidation. The antioxidant or pro-oxidant activity of fractions increased with the con-centration. The phenol polarity and the technological process to manufacture cranberry juice can influence the antioxidant and antiradical activities of fractions.
基金supported by the Creative Inquiry Fund at Clemson University to Y.D.China National Science and Technology Major Projects for Key New Drugs Innovation(No.2012ZX09103301-018)
文摘Cranberry extract(CBE) rich in polyphenols are potent to delay paralysis induced by alleviating β-amyloid(Aβ) toxicity in C. elegans model of Alzheimer's disease(AD). In order to better apply CBE as an anti-AD agent efficiently, we sought to deterrmine whether preventive or therapeutic effect contributes more prominently toward CBE's anti-AD activity. As the level of Aβtoxicity and memory health are two major pathological parameters in AD, in the present study, we compared the effects of CBE on Aβtoxicity and memory health in the C. elegans AD model treated with preventive and therapeutic protocols. Our results revealed that CBE prominently showed the preventive efficacy, providing a basis for further investigation of these effects in mammals.
基金supported by the United States Department of Agriculture Specialty Crop Research Initiative(SCRI)2008-51180-04878(NV)grant,New Jersey Agricultural Experiment Station and Ocean Spray Cranberries,Inc.
文摘Culturable fungal population diversity and succession was investigated in developing cranberry ovaries of fruit rot-resistant and rot-susceptible cranberry selections,from flower through mature fruit.Fungi were recovered in culture from 1185 of 1338 ovary tissues collected from June to September,yielding 2255 isolates that represented 42 morphotaxa.During the season,species richness varied from 2 to 17 and 2 to 18 in rot-resistant and rotsusceptible selections,respectively,increasing from wk1 to wk10 and then gradually declining to wk14.Shannon-Wiener diversity index varied from 0.27 to 2.32 in rotresistant and 0.18 to 2.38 in rot-susceptible,and Pielou’s evenness index varied from 0.11 to 0.63 and 0.06 to 0.64 in rot-resistant and rot-susceptible selections,respectively,confirming that diversity of fungi in developing ovaries was similar among rot-resistant and rot-susceptible selections,but varied among sampling time points.Principal component analysis grouped samples collected at the same sampling time point together regardless of rot susceptibility of the selections,and detected the predominant fungal species associated with each stage of development.Successional changes were observed in populations of endophytic,pathogenic and saprophytic fungi throughout the season as ovaries matured.
文摘Fuzzy chromatography mass spectrometric(FCMS)fingerprinting methods in combination with principal component analysis(PCA)and soft independent modeling by class analogy(SIMCA)were developed for phenolic profiling and differentiation of American cranberry cultivars.Two FCMS fingerprinting methods,ion mobility fuzzy chromatography mass spectrometric(imFCMS)and conventional FCMS,are compared in the study.PCA and SIMCA successfully differentiated the cultivars with both methods.The six cultivars used formed three distinct groups in the PCA score plots,and each group contained one wild selection cultivar and its genetically related hybrid(s).Compared with FCMS fingerprinting,imFCMS fingerprinting provided better intra-cultivar sample clustering and inter-cultivar sample separation in PCA and superior sample classifica-tion sensitivity in SIMCA.Ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-HRMS)analysis was conducted on selected samples to profile the phenolic difference between the cultivars.Compound identification using UPLC-HRMS revealed that flavonoid compounds,including flavonol glycosides,proanthocyanidins and anthocyanins,are major components that contribute to the phenolic profile variation among cultivars.
文摘Many food and plant extracts have shown in vitro antiHelicobacter pylori(H.pylori)activity,but are less effective in vivo.The anti-H.pylori effects of these extracts are mainly permeabilitization of the membrane,anti-adhesion,inhibition of bacterial enzymes andbacterial grown.We,herein,review treatment effects of cranberry,garlic,curcumin,ginger and pistacia gum against H.pylori in both in vitro,animal studies and in vivo studies.
文摘Objective:Despite conflicting evidence,it is common practice to use continuous antibiotic prophylaxis(CAP)in patients with indwelling double-J(DJ)stents.Cranberry extracts and d-mannose have been shown to prevent colonization of the urinary tract.We evaluated their role in this setting.Methods:We conducted a prospective randomized study to evaluate patients with indwelling DJ stents following urological procedures.They were randomized into three groups.Group A(n=46)received CAP(nitrofurantoin 100 mg once daily[OD]).Group B(n=48)received cranberry extract 300 mg and d-mannose 600 mg twice daily(BD).Group C(n=40)received no prophylaxis.The stents were removed between 15 days and 45 days after surgery.Three groups were compared in terms of colonization of stent and urine,stent related symptoms and febrile urinary tract infections(UTIs)during the period of indwelling stent and until 1 week after removal.Results:In Group A,9(19.5%)patients had significant bacterial growth on the stents.This was 8(16.7%)in the Group B and 5(12.5%)in Group C(p-value:0.743).However,the culture positivity rate of urine specimens showed a significant difference(p-value:0.023)with Group B showing least colonization of urine compared to groups A and C.There was no statistically significant difference in the frequency of stent related symptoms(p-value:0.242)or febrile UTIs(p-value:0.399)among the groups.Conclusion:Prophylactic agents have no role in altering bacterial growth on temporary indwelling DJ stent,stent related symptoms or febrile UTIs.Cranberry extract may reduce the colonization of urinary tract,but its clinical significance needs further evaluation.
文摘There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cranberry, kidney, otebo, pink, pinto, Small Red Mexican and white bean to the pendimethalin applied preplant incorporated at 1080 and 2160 g.a.i.ha-1. Pendimethalin PPI caused minimal injury in most market classes of dry bean at 1 and 2 WAE. There was no injury in various market classes of dry bean with the low dose at 1 and 2 weeks after emergence (WAE). However, at the high dose there was 0 to 4% injury at 1 WAE and 0 to 7% injury at 2 WAE in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Pendimethalin PPI was more injurious in white bean than in black, cranberry, kidney, otebo, pink, pinto and SRM bean. Pink and SRM bean exhibited the most tolerance to pendimethalin applied PPI at 1080 g.ai.ha-1 or 2160 g.ai.ha-1. Pendimethalin caused no adverse effect on plant height, shoot dry weight, seed moisture content and seed yield of black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Based on these results, there is an adequate margin of crop safety for pendimethalin applied PPI at the proposed dose of 1080 g.ai.ha-1 in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean in Ontario.
文摘Eight field trials were conducted from 2006 to 2008 at various locations in Ontario to evaluate the co-application of postemergence herbicides with cyhalothrin-lambda or dimethoate insecticides in cranberry and white bean. At 2 weeks after treatment, the addition of cyhalothrin-lambda or dimethoate insecticides to sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen did not increase injury at the Exeter and Ridgetown locations except for bentazon plus dimethoate which caused greater injury than bentazon alone (2.9% vs 0.2%) in 2006. However at Harrow, the addition of dimethoate to quizalofop-p-ethyl increased injury (0% vs 4.9%) in 2007 and the addition cyhalothrin-lambda or dimethoate to sethoxydim increased injury in 2008 in dry bean. The addition of cyhalothrin-lambda to quizalofop-p-ethyl also increased injury (0% vs 4.5%) in 2008. There was no adverse effect on dry bean injury with other treatments at Harrow in 2007 or 2008. The addition of cyhalothrin-lambda or dimethoate to the herbicides evaluated did not have any adverse effect on plant height, shoot dry weight or yield of dry bean except for bentazon plus dimethoate which decreased shoot dry weight 20% compared to bentazon alone at Harrow in 2008. Based on these results, cyhalothrin-lambda or dimethoate can be tank-mixed with sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen when the optimum application timing of these herbicides and insecticides coincide.
文摘The effect of biostimulants such as Crop Booster and RR SoyBooster on dry bean under Ontario environmental conditions is not known. A total of 12 field experiments (6 in cranberry bean “Etna” and 6 in white bean “OAC REX”) were conducted at two locations (Ridgetown and Exeter, Ontario, Canada) to evaluate the effect of Crop Booster and RR SoyBooster on visible injury, shoot dry weight, height and yield of cranberry and white bean. Visible injury ranged from 0% to 7.3% in white bean and 0% to 9.4% in cranberry bean with quizalofop-p-ethyl, bentazon, fomesafen, bentazon plus fomesafen, imazethapyr and imazethapyr plus bentazon alone or in combination with Crop Booster and RR SoyBooster. The addition of Crop Booster or RR SoyBooster to herbicides evaluated had no significant effect on shoot dry weight, height, seed moisture content and yield of white or cranberry bean except with the addition of RR SoyBooster to quizalofop-p-ethyl which increased height 3.7% and the addition of the Crop Booster to bentazon which decreased shoot dry weight 12% and the addition of Crop Booster to bentazon plus fomesafen which increased shoot dry weight 17% in white bean. Based on these results, there were minimal effects from the addition of Crop Booster or RR SoyBooster to commonly used herbicides in white and cranberry bean.
文摘This review provides an examination of the marsh spot disease in beans and the roles played by its causal factor, manganese (Mn) deficiency. The discovery of the marsh spot disease, its relation with Mn deficiency, and how it can be treated are discussed. Mn serves as a cofactor and a catalyst in various metabolic processes in different cell compartments, such as the oxygen-evolving complex of photosystem II (PSII) or reactive oxygen species scavenging. Some major quantitative trait loci (QTL) and putative candidate genes associated with Mn content in plants, especially in plant seeds, have been identified. Marsh spot disease in cranberry common bean is controlled by several major genes with significant additive and epistatic effects. They provide valuable clues for QTL candidate gene prediction and an improved understanding of the genetic mechanisms responsible for marsh spot resistance in plants.
