This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro.The total phenol and flavonoid contents of the extracts were determined using c...This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro.The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector(HPLC-DAD).The effects of the extracts on Fe^2+-induced lipid peroxidation in rats’brain homogenate,monoamine oxidase(MAO),Na^+/K^+-ATPase,acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)activities were also assessed.The aqueous extract had higher total phenol and flavonoid contents than the methanol extract.HPLC-DAD revealed that quercetin ellagic,chlorogenic and caffeic acids were the most abundant phenolic compounds in the leaves.The aqueous extract had higher inhibitory effects on MAO,AChE and BChE activities while there was no significant difference between their Fe^2+-induced lipid peroxidation inhibitory effects.Furthermore,both extracts stimulated Na^+/K^+-ATPase activity;however,methanol extract had higher stimulatory effect.The neuroprotective properties of H.crinita leaves could be associated with its inhibitory effects on Fe2+-induced lipid peroxidation and modulation of MAO,Na^+/K^+-ATPase,AChE,and BChE activities.Therefore,H.crinita leaves could be used as a functional food and dietary intervention for the management of some neurodegenerative diseases.Nevertheless,the aqueous extracts exhibited better neuroprotective properties.展开更多
Objective:To evaluate in vitro antimicrobial,antioxidant activities and total phenol contents of Cystoseira crinita and Ulva intestinalis species collected from the coastal region of Sinop.Methods:The antimicrobial ac...Objective:To evaluate in vitro antimicrobial,antioxidant activities and total phenol contents of Cystoseira crinita and Ulva intestinalis species collected from the coastal region of Sinop.Methods:The antimicrobial activity of each methanolic algae sample was screened by using disc diffusion method against to 15 bacteria and 3 yeasts.The antioxidant potential of the extracts on the stable radical 1,1-diphenyl-2-picrylhydrazyl was determined.The total phenolic content of the 3 methanolic extracts of the seaweed samples were determined by the Folin-Ciocalteu method.Results:The results of antimicrobial assay showed that extracts were more effective against Gram-positive bacteria rather than Gram-negative.In addition,while the algal extracts had antifungal efficacy against Candida krusei,the other yeast strains were not affected at all.According to the findings of antioxidant activity,all methanolic extracts displayed good free radical scavenging activity ranging from IC_(50)=(32.19±0.08)mg/mL to the IC_(50)=(37.57±0.11)mg/mL.The total phenols content of the macroalgal extracts were found as between(5.10±0.16)mg gallic acid equivalent/g and(87.70±1.03)mg gallic acid equivalent/g.In this sense,our findings confirmed that there was a positive linear correlations(r=0.86)between total phenol contents and the IC_(50) values.Conclusions:The data gathered from this study suggested that the seaweeds can be used as a potential natural seafood sources owing to the antimicrobial efficiency and good antioxidant activity.展开更多
文摘This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro.The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector(HPLC-DAD).The effects of the extracts on Fe^2+-induced lipid peroxidation in rats’brain homogenate,monoamine oxidase(MAO),Na^+/K^+-ATPase,acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)activities were also assessed.The aqueous extract had higher total phenol and flavonoid contents than the methanol extract.HPLC-DAD revealed that quercetin ellagic,chlorogenic and caffeic acids were the most abundant phenolic compounds in the leaves.The aqueous extract had higher inhibitory effects on MAO,AChE and BChE activities while there was no significant difference between their Fe^2+-induced lipid peroxidation inhibitory effects.Furthermore,both extracts stimulated Na^+/K^+-ATPase activity;however,methanol extract had higher stimulatory effect.The neuroprotective properties of H.crinita leaves could be associated with its inhibitory effects on Fe2+-induced lipid peroxidation and modulation of MAO,Na^+/K^+-ATPase,AChE,and BChE activities.Therefore,H.crinita leaves could be used as a functional food and dietary intervention for the management of some neurodegenerative diseases.Nevertheless,the aqueous extracts exhibited better neuroprotective properties.
文摘Objective:To evaluate in vitro antimicrobial,antioxidant activities and total phenol contents of Cystoseira crinita and Ulva intestinalis species collected from the coastal region of Sinop.Methods:The antimicrobial activity of each methanolic algae sample was screened by using disc diffusion method against to 15 bacteria and 3 yeasts.The antioxidant potential of the extracts on the stable radical 1,1-diphenyl-2-picrylhydrazyl was determined.The total phenolic content of the 3 methanolic extracts of the seaweed samples were determined by the Folin-Ciocalteu method.Results:The results of antimicrobial assay showed that extracts were more effective against Gram-positive bacteria rather than Gram-negative.In addition,while the algal extracts had antifungal efficacy against Candida krusei,the other yeast strains were not affected at all.According to the findings of antioxidant activity,all methanolic extracts displayed good free radical scavenging activity ranging from IC_(50)=(32.19±0.08)mg/mL to the IC_(50)=(37.57±0.11)mg/mL.The total phenols content of the macroalgal extracts were found as between(5.10±0.16)mg gallic acid equivalent/g and(87.70±1.03)mg gallic acid equivalent/g.In this sense,our findings confirmed that there was a positive linear correlations(r=0.86)between total phenol contents and the IC_(50) values.Conclusions:The data gathered from this study suggested that the seaweeds can be used as a potential natural seafood sources owing to the antimicrobial efficiency and good antioxidant activity.
