Large oilfields are often coincidentally located in major river deltas and wetlands,and potentially damage the structure,function and ecosystem service values of wetlands during oil exploration.In the present study,th...Large oilfields are often coincidentally located in major river deltas and wetlands,and potentially damage the structure,function and ecosystem service values of wetlands during oil exploration.In the present study,the effects of crude oil contamination during oil exploration on soil physical and chemical properties were investigated in marshes of the Momoge National Nature Reserve in Jilin Province,China.The concentrations of total petroleum hydrocarbons in the marsh soil near the oil wells are significantly higher than those in the adjacent control marsh.Soil water contents in oil-contaminated marshes are negatively correlated with soil temperature and are significantly lower than those in the control area,especially in fall.Crude oil contamination significantly increases the soil pH up to8.0,and reduces available phosphorus concentrations in the soil.The concentrations of total organic carbon are significantly different among sampling sites.Therefore,crude oil contamination could potentially alkalinize marsh soils,adversely affect soil fertility and physical properties,and cause deterioration of the marshes in the Momoge National Nature Reserve.Phyto-remediation by planting Calamagrostis angustifolia has the potential to simultaneously restore and remediate the petroleum hydrocarbon-contaminated wetlands.Crude oil contamination affects the soil physical and chemical properties,so developing an effective restoration program in the Momoge wetland is neccesary.展开更多
Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, ...Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, 1-1-200 and 150-1-5）, wheat （Triticum aestivum L.） and garden cress （Lipidium sativum L.）. This experiment was conducted in laboratory at room temperature. Oil extracted from crude oil contaminated soil was added to sand used in different concentrations （0%, 2%, 4%, 6%, 8% and 10%）. 10 seeds of each crop were sown in ptri-dishes with different treatments separately. For Each treatment four replicates were used in completely randomized design, germination percent was calculated, after one week, shoot and root length were measured. Data were statistically analyzed using MINTAB version 12. The results show that barley mutant line （150-2-3） was significantly different from other entries in germination percent, shoot and root length except mutant line 150-1-5 in germination percent. Results demonstrated that the mutant line 150-2-3 was promising to remediate crude oil contaminated soil.展开更多
Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal r...Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal restriction fragment length polymorphism （T-RFLP） and clone library analyses.The 140-day microcosm experiments showed that low C：N：P ratio,high availability of surfactant and addition of bulking agent significantly enhanced the performance,leading to the highest total petroleum hydrocarbon removal.Meanwhile,the bacterial community was remarkably changed by the multiple biostimulation treatments,with the Deltaproteobacteria,Firmicutes,Actinobacteria,Acidobacteria and Planctomycetes being inhibited and the Alphaand Beta-proteobacteria and some unknown Gammaproteobacteria bacteria being enriched.In addition,different hydrocarbon-degraders came to power in the following turn.At the first stage,the Alcanivorax-related Gammaproteobacteria bacteria dominated in the biostimulated soil and contributed mainly to the biodegradation of easily degradable portion of the heavy crude oil.Then the bacteria belonging to Alphaproteobacteria,followed by bacteria belonging to Candidate division OD1,became the dominant oil-degraders to degrade the remaining recalcitrant constituents of the heavy crude oil.展开更多
基金Under the auspices of National Natural Science Foundation of China(No.31170476)Chinese Academy of Sciences/State Administration of Foreign Experts Affairs International Partnership Program for Creative Research Teams(No.KZZD-EW-TZ-07)+1 种基金Major Program of Science and Technology of Jilin Province(No.20075014)Fundamental Research Funds for the Central Universities of China(No.11GJHZ002)
文摘Large oilfields are often coincidentally located in major river deltas and wetlands,and potentially damage the structure,function and ecosystem service values of wetlands during oil exploration.In the present study,the effects of crude oil contamination during oil exploration on soil physical and chemical properties were investigated in marshes of the Momoge National Nature Reserve in Jilin Province,China.The concentrations of total petroleum hydrocarbons in the marsh soil near the oil wells are significantly higher than those in the adjacent control marsh.Soil water contents in oil-contaminated marshes are negatively correlated with soil temperature and are significantly lower than those in the control area,especially in fall.Crude oil contamination significantly increases the soil pH up to8.0,and reduces available phosphorus concentrations in the soil.The concentrations of total organic carbon are significantly different among sampling sites.Therefore,crude oil contamination could potentially alkalinize marsh soils,adversely affect soil fertility and physical properties,and cause deterioration of the marshes in the Momoge National Nature Reserve.Phyto-remediation by planting Calamagrostis angustifolia has the potential to simultaneously restore and remediate the petroleum hydrocarbon-contaminated wetlands.Crude oil contamination affects the soil physical and chemical properties,so developing an effective restoration program in the Momoge wetland is neccesary.
文摘Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, 1-1-200 and 150-1-5）, wheat （Triticum aestivum L.） and garden cress （Lipidium sativum L.）. This experiment was conducted in laboratory at room temperature. Oil extracted from crude oil contaminated soil was added to sand used in different concentrations （0%, 2%, 4%, 6%, 8% and 10%）. 10 seeds of each crop were sown in ptri-dishes with different treatments separately. For Each treatment four replicates were used in completely randomized design, germination percent was calculated, after one week, shoot and root length were measured. Data were statistically analyzed using MINTAB version 12. The results show that barley mutant line （150-2-3） was significantly different from other entries in germination percent, shoot and root length except mutant line 150-1-5 in germination percent. Results demonstrated that the mutant line 150-2-3 was promising to remediate crude oil contaminated soil.
基金supported by the International Cooperation Project of National Natural Science Foundation of China (No.40821140541)the National High Technology Research and Development Program (863) of China (No.2009AA063501)
文摘Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal restriction fragment length polymorphism （T-RFLP） and clone library analyses.The 140-day microcosm experiments showed that low C：N：P ratio,high availability of surfactant and addition of bulking agent significantly enhanced the performance,leading to the highest total petroleum hydrocarbon removal.Meanwhile,the bacterial community was remarkably changed by the multiple biostimulation treatments,with the Deltaproteobacteria,Firmicutes,Actinobacteria,Acidobacteria and Planctomycetes being inhibited and the Alphaand Beta-proteobacteria and some unknown Gammaproteobacteria bacteria being enriched.In addition,different hydrocarbon-degraders came to power in the following turn.At the first stage,the Alcanivorax-related Gammaproteobacteria bacteria dominated in the biostimulated soil and contributed mainly to the biodegradation of easily degradable portion of the heavy crude oil.Then the bacteria belonging to Alphaproteobacteria,followed by bacteria belonging to Candidate division OD1,became the dominant oil-degraders to degrade the remaining recalcitrant constituents of the heavy crude oil.
