期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
RASSF1A和SHOX2基因甲基化检测联合快速现场细胞学评价对肺癌的诊断价值 被引量:12
1
作者 任雪珠 张树森 +4 位作者 蔡志刚 李海涛 许晓岚 齐天杰 李宏林 《天津医药》 CAS 北大核心 2022年第3期319-323,共5页
目的评估RAS相关区域家族1A(RASSF1A)联合矮小同源盒基因2(SHOX2)基因甲基化检测和快速现场细胞学评价(C-ROSE)对肺癌的诊断价值。方法选取行RASSF1A和SHOX2甲基化检测并有明确病理诊断的179例可疑肺癌患者,其中肺癌58例,肺良性病变121... 目的评估RAS相关区域家族1A(RASSF1A)联合矮小同源盒基因2(SHOX2)基因甲基化检测和快速现场细胞学评价(C-ROSE)对肺癌的诊断价值。方法选取行RASSF1A和SHOX2甲基化检测并有明确病理诊断的179例可疑肺癌患者,其中肺癌58例,肺良性病变121例。179例中114例(肺癌45例,肺良性病变69例)通过迪夫快速染色完成C-ROSE检测。以病理结果为金标准,分析RASSF1A、SHOX2甲基化和(或)C-ROSE诊断肺癌的敏感度及特异度,同时明确不同标本类型和不同肿瘤类型中RASSF1A和SHOX2甲基化的结果与病理诊断的一致性。结果RASSF1A联合SHOX2甲基化检测的敏感度为77.59%(45/58),特异度为80.17%(97/121)。亚组分析中,肺穿刺活检及肺泡灌洗液标本中甲基化与病理诊断一致率较好,分别为88.8%(8/9)、79.75%(126/158),而胸水的一致率稍差,为63.64%(7/11)。小细胞肺癌和鳞癌甲基化检测准确率分别为100%(5/5)、92.31%(12/13),肺腺癌为66.67%(18/27)。C-ROSE检测的敏感度为75.56%(34/45),特异度为95.65%(66/69)。甲基化检测联合C-ROSE的敏感度为86.96%(39/45),特异度为86.67%(60/69)。结论RASSF1A、SHOX2甲基化检测及C-ROSE用于肺癌的诊断均具有较高的敏感度和特异度,两者结合将成为病理学诊断的有效的补充手段,两者结合有助于进一步提高诊断肺癌的效能。 展开更多
关键词 肺肿瘤 DNA甲基化 诊断 RAS相关区域家族1A 矮小同源盒基因2 快速现场细胞学评价
下载PDF
ROSE: Alternative for Cancelled and Inconclusive Cytopathologic Examinations, as Well as Professional Training at the UNESP-Botucatu Veterinary Hospital
2
作者 Fabiane Andrade Correia Neiva Eduardo Eburnio +2 位作者 Paula de Sanctis Nayara Maria Gil Mazzante Noeme Sousa Rocha 《Open Journal of Veterinary Medicine》 2019年第9期121-135,共15页
ROSE is a method for fast sample evaluation that does not compromise sensitivity and specificity in the hospital environment when establishing the diagnosis and the therapeutic protocol. The technique is already well-... ROSE is a method for fast sample evaluation that does not compromise sensitivity and specificity in the hospital environment when establishing the diagnosis and the therapeutic protocol. The technique is already well-established in human medicine as it operates within the standard operational procedure, reducing the rates of inconclusive results and cancelled cytopathology assays in the hospital environment. However, its application is scarce in Veterinary Medicine, including in hospitals where intensive care is available. This study aims at conducting a case-by-case assessment of inconclusive and cancelled examinations in the Cytopathology Outpatient Clinic at UNESP (HV), Botucatu, Brazil, from 2012 to 2016 and ascertains the causes. For this purpose, a retrospective study was conducted for 9587 examinations in canines, of which 4.1% and 10.44% were inconclusive and cancelled, respectively. These results are not in line with ROSE, which foresees a total rate of 5% for these occurrences. The reasons for these high rates in the outpatient clinic were the lack of a Standard Operating Procedure (SOP) and the relative inexperience of the residents in a university hospital. Therefore, with the adoption of the ROSE protocol for veterinary cytopathological examinations, together with adequate training for the outpatient professionals responsible for the examinations, the goals set forth by the ROSE protocol would be met and the rates of inconclusive and cancelled examinations would decrease considerably. Lastly, this study hopes to contribute towards the diagnosis and therapeutic protocols of the main diseases affecting dogs. 展开更多
关键词 PAAF Inconclusive or Canceled cytologY Canine ROSE rapid on-site Evaluation
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部