The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. ...The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. The results showed that the content of GA3 in efflux extracted with alcohol is 10% higher than that with distilled water. With the increase of the durations of extraction, the content of GA3 increases and the dissolution of pigments also increase. For extraction of GA3 cultured in solid medium, the best decolorizing result was obtained when it was extracted with 75%-95% alcohol in static way for 1-3 h, or in a vibrating way for 30-60 min, and then to decolor in a static way for 30-60 min in 122 resin column.展开更多
Aspergillus ficuum was immobilized with sodium alginate, and decolourization of Reactive Brilliant Blue KN-R was studied on immobilized and free Aspergillus ficuum. The optimal preparation condition of the strain immo...Aspergillus ficuum was immobilized with sodium alginate, and decolourization of Reactive Brilliant Blue KN-R was studied on immobilized and free Aspergillus ficuum. The optimal preparation condition of the strain immobilization was obtained by the orthogonal test, it is sodium alginate 3%, CaCl_2 5%, wet mycelia 30 g/L, calcific time 8 h. It was found that the immobilized cells could effectively decolourize Reactive Brilliant Blue KN-R, the optimum temperature and pH were 33℃ and 5.0, respectively. The kinetics study of decolourization of immobilized cells showed that the decolourization of Aspergillus ficuum immobilized conformed to zero-order reaction model. The decolourization efficiency of immobilized cell compared with that of free cell in different physical conditions. Results showed that the decolourization of immobilized cells with mycelia had the best efficiency. The immobilized cells could be reused after the first decolourization.展开更多
This paper explores a new compounding coagulant for decolourization, which can decolour wastewater containing soluble dyestuffs (especially reactive and acidic dyestuffs). The best prescription and condition of this d...This paper explores a new compounding coagulant for decolourization, which can decolour wastewater containing soluble dyestuffs (especially reactive and acidic dyestuffs). The best prescription and condition of this decolourizing process are discussed and the mechanism is studied. Experimental results show that the treatment efficiency of decolourization is very high by using the new coagulant. Both the colour and pH of the effluent meet the required standards. The synergism of chemical and physico-chemical actions among the coagulant components has also been found. This effect determines the superiority of the new coagulant to other single component ones.展开更多
Several wood rotting fungi decolourized Remazol brilliant blue R (RBBR) and carminic acid (CA). Parallel activity of laccase in these fungi was studied. The addition of acetovanillone (AV) or acetosyringone (AS) inten...Several wood rotting fungi decolourized Remazol brilliant blue R (RBBR) and carminic acid (CA). Parallel activity of laccase in these fungi was studied. The addition of acetovanillone (AV) or acetosyringone (AS) intensified these processes: decolourization was more extensive than in the experiment omitting these compounds. At the presence of AS the decourization was more extensive than AV. However the level of decolorizing was relatively low in comparison to laccase activity on syringaldazine. The highly purified constitutive form of Cerrena unicolor and inducible form of Trametes versicolor laccases also destained both dyes. Anyway the addition of AV and AS improved the efficiency of dyes decolourization by wood rotting fungi and fungal laccase. Nitrogen starvation induced the laccase and decoloration activity in both organisms, irrespective of nitrogen availability. This fact indicates laccase not solely responsible for discoloration, and probably discoloration of dyes involves more than one mechanism.展开更多
The degradation mechanism of Cationic Red X-GRL was investigated when the intermediates, the nitrate ion and the pH were analyzed in the ozonation. The degradation of the Cationic Red X-GRL includes the de-auxochrome...The degradation mechanism of Cationic Red X-GRL was investigated when the intermediates, the nitrate ion and the pH were analyzed in the ozonation. The degradation of the Cationic Red X-GRL includes the de-auxochrome stage, the decolour stage, and the decomposition of fragment stage. During the degradation process, among the six nitrogen atoms of Cationic Red X-GRL, one is transferred into a nitrate ion, one becomes the form of an amine compound, and the rest four are transformed into two molecules of nitrogen. In the course of the ozonation of Cationic Red X-GRL, the direct attack of ozone is the main decolour effect.展开更多
文摘The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. The results showed that the content of GA3 in efflux extracted with alcohol is 10% higher than that with distilled water. With the increase of the durations of extraction, the content of GA3 increases and the dissolution of pigments also increase. For extraction of GA3 cultured in solid medium, the best decolorizing result was obtained when it was extracted with 75%-95% alcohol in static way for 1-3 h, or in a vibrating way for 30-60 min, and then to decolor in a static way for 30-60 min in 122 resin column.
文摘Aspergillus ficuum was immobilized with sodium alginate, and decolourization of Reactive Brilliant Blue KN-R was studied on immobilized and free Aspergillus ficuum. The optimal preparation condition of the strain immobilization was obtained by the orthogonal test, it is sodium alginate 3%, CaCl_2 5%, wet mycelia 30 g/L, calcific time 8 h. It was found that the immobilized cells could effectively decolourize Reactive Brilliant Blue KN-R, the optimum temperature and pH were 33℃ and 5.0, respectively. The kinetics study of decolourization of immobilized cells showed that the decolourization of Aspergillus ficuum immobilized conformed to zero-order reaction model. The decolourization efficiency of immobilized cell compared with that of free cell in different physical conditions. Results showed that the decolourization of immobilized cells with mycelia had the best efficiency. The immobilized cells could be reused after the first decolourization.
文摘This paper explores a new compounding coagulant for decolourization, which can decolour wastewater containing soluble dyestuffs (especially reactive and acidic dyestuffs). The best prescription and condition of this decolourizing process are discussed and the mechanism is studied. Experimental results show that the treatment efficiency of decolourization is very high by using the new coagulant. Both the colour and pH of the effluent meet the required standards. The synergism of chemical and physico-chemical actions among the coagulant components has also been found. This effect determines the superiority of the new coagulant to other single component ones.
文摘Several wood rotting fungi decolourized Remazol brilliant blue R (RBBR) and carminic acid (CA). Parallel activity of laccase in these fungi was studied. The addition of acetovanillone (AV) or acetosyringone (AS) intensified these processes: decolourization was more extensive than in the experiment omitting these compounds. At the presence of AS the decourization was more extensive than AV. However the level of decolorizing was relatively low in comparison to laccase activity on syringaldazine. The highly purified constitutive form of Cerrena unicolor and inducible form of Trametes versicolor laccases also destained both dyes. Anyway the addition of AV and AS improved the efficiency of dyes decolourization by wood rotting fungi and fungal laccase. Nitrogen starvation induced the laccase and decoloration activity in both organisms, irrespective of nitrogen availability. This fact indicates laccase not solely responsible for discoloration, and probably discoloration of dyes involves more than one mechanism.
文摘The degradation mechanism of Cationic Red X-GRL was investigated when the intermediates, the nitrate ion and the pH were analyzed in the ozonation. The degradation of the Cationic Red X-GRL includes the de-auxochrome stage, the decolour stage, and the decomposition of fragment stage. During the degradation process, among the six nitrogen atoms of Cationic Red X-GRL, one is transferred into a nitrate ion, one becomes the form of an amine compound, and the rest four are transformed into two molecules of nitrogen. In the course of the ozonation of Cationic Red X-GRL, the direct attack of ozone is the main decolour effect.
