Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction o...Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction of bridge pile foundations,accidents such as hole collapse,slurry leakage,and drill sticking will easily occur.In this paper,the principle and method of sonar detection for detecting karst caves at the bottom of bridge piles was introduced,and the sonar detection data and the cave situation at the bottom of the pile during the construction process in combination with the case of Yunnan Zhenguo Highway Project was analyzed,which verifies the practicability and reliability of sonar detection method reliability.展开更多
SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative proba...SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative probabilities between the number of anticyclonic/cyclonic ME fronts(AEF/CEF)and the number of anticyclones/cyclones reached 20%.The northeastern and southwestern parts of these anticyclones had more fronts than the northwestern and southeastern parts,although CEFs were nearly equally distributed in all directions.The number of ME fronts had remarkable seasonal variations,while the eddy kinetic energy(EKE)showed no seasonal variations.The total EKE at the ME fronts was three times of that within the MEs,and it was much stronger in AEFs than in CEFs.The interannual variability in the number of ME fronts and EKE had no significant correlation with the El Ni?o-Southern Oscillation(ENSO)index.Possible mechanisms of ME fronts were discussed,but the contributions of mesoscale eddies to SST fronts need to be quantified in future studies.展开更多
To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entr...To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entropy of content name of interest packet.On the one hand,taking advantage of the characteristics of self-similarity is very sensitive to traffic changes,calculating the Hurst index of the traffic,to identify initial IFA attacks.On the other hand,according to the randomness of user requests,calculating the information entropy of content name of the interest packets,to detect the severity of the IFA attack,is.Finally,based on the above two aspects,we use the bilateral detection method based on non-parametric CUSUM algorithm to judge the possible attack behavior in CCN.The experimental results show that flooding attack detection method proposed for CCN can not only detect the attack behavior at the early stage of attack in CCN,but also is more accurate and effective than other methods.展开更多
Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems a...Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems are essentially required due to lack of effective vaccine against dengue virus and the progressive spread of the dengue virus infection.The recent progress in developing micro-and nano-fabrication techniques has led to low cost and scale down the biomedical point-of-care devices.Starting from the conventional and modern available methods for the diagnosis of dengue infection,this review examines several emerging rapid and point-of-care diagnostic devices that hold significant potential for the progress in smart diagnosis tools.The given review revealed that an effective vaccine is required urgently against all the dengue virus serotypes.However,the rapid detection methods of dengue virus help in early treatment and significantly reduce the dengue virus outbreak.展开更多
Mycotoxins are widely distributed in various feed ingredients and animal feed. Feed contamination by mycotoxin leads to deteriorated feed,various diseases of animals and even acute death,bringing great loss to breedin...Mycotoxins are widely distributed in various feed ingredients and animal feed. Feed contamination by mycotoxin leads to deteriorated feed,various diseases of animals and even acute death,bringing great loss to breeding industry. The article summarized the harm of mycotoxin to animal and detection methods in feed and animal,in order to provide a reference for further improving mycotoxin detection methods and reducing the harm of mycotoxin to breeding industry.展开更多
The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomati...The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomatic infections is increasing as COVID-19 research continues and SARS-CoV-2 testing improves.Many asymptomatic COVID-19 patients with no obvious clinical symptoms are able to transmit the virus to others due to the presence of SARS-CoV-2 in their bodies,thus greatly accelerating the worldwide pandemic and posing great difficulty and threat to global epidemic prevention and control.Therefore,early detection and identification of asymptomatic infected persons through various testing methods are effective measures to control the COVID-19 pandemic.This article will provide a brief review of the available methods and techniques for detecting asymptomatic COVID-19 patients.展开更多
Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great ...Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great harm to the breeding industry.In this paper,the harm of mycotoxin to animals and its detection methods in feed and animals are summarized and analyzed,so as to provide the reference basis for further improving the detection methods of mycotoxin and reducing the harm of mycotoxin to breeding industry.展开更多
In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators hav...In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.展开更多
Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foo... Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.……展开更多
Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell the...Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell therapy,a promising branch of adoptive T-cell therapy,has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer.At present,CAR-T cells are predominantly used to treat hematological malignancies,although their application in solid tumors is being readily investigated.Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy,few have evaluated predictors of CAR-T cell therapeutic efficacy.Thus,the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy,with a particular focus on biomarkers and their detection methods.展开更多
Simultaneous localization and mapping(SLAM) technology is a research hotspot in the field of intelligent mobile robot, and many researchers have developed many classic systems in the past few decades. However, most of...Simultaneous localization and mapping(SLAM) technology is a research hotspot in the field of intelligent mobile robot, and many researchers have developed many classic systems in the past few decades. However, most of the existing SLAM methods assume that the environment of the robot is static, which results in the performance of the system being greatly reduced in the dynamic environment. To solve this problem, a new dynamic object detection method based on point cloud motion analysis is proposed and incorporated into ORB-SLAM2. First, the method is regarded as a preprocessing stage, detecting moving objects in the scene, and then removing the moving objects to enhance the performance of the SLAM system. Experiments performed on a public RGB-D dataset show that the motion cancellation method proposed in this paper can effectively improve the performance of ORB-SLAM2 in a highly dynamic environment.展开更多
Most multiphase flow separation detection methods used commonly in oilfields are low in efficiency and accuracy,and have data delay.An online multiphase flow detection method is proposed based on magnetic resonance te...Most multiphase flow separation detection methods used commonly in oilfields are low in efficiency and accuracy,and have data delay.An online multiphase flow detection method is proposed based on magnetic resonance technology,and its supporting device has been made and tested in lab and field.The detection technology works in two parts:measure phase holdup in static state and measure flow rate in flowing state.Oil-water ratio is first measured and then gas holdup.The device is composed of a segmented magnet structure and a dual antenna structure for measuring flowing fluid.A highly compact magnetic resonance spectrometer system and intelligent software are developed.Lab experiments and field application show that the online detection system has the following merits:it can measure flow rate and phase holdup only based on magnetic resonance technology;it can detect in-place transient fluid production at high frequency and thus monitor transient fluid production in real time;it can detect oil,gas and water in a full range at high precision,the detection isn’t affected by salinity and emulsification.It is a green,safe and energy-saving system.展开更多
In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detect...In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detection method for the genetically modified( GM) maize MON88017 using real-time fluorescent quantitative PCR. Meanwhile,the method is evaluated by several methodological indicators such as specificity,sensitivity,accuracy and uncertainty of measurement. The results show that the method has strong specificity in analysis of genetically modified maize MON88017. The mean value(1. 54%) repeatedly measured for 29 times with the relative deviation of 2. 7% was close to the real value(1. 50%) and the variation coefficient of the measured value was 0. 1. The tested recovery rate is 100% and the uncertainty of measurement is 0. 096. 5 copies of the MON88017 molecular fragment can be detected at 97. 5% confidence level. Consequently,the quantitative detection method established in this paper for the GM maize MON88017 has fairly high specificity,accuracy and sensitivity and this technology established in this paper can provide good technical support for the safety supervision of genetically modified organisms in China.展开更多
<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as...<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as an infectious agent. These microorganisms are generally transmitted in fecal-contaminated water. However, <i>E. dispar</i> present in industrial wastewater is also capable of creating biofilms that can cause adverse impacts in piping networks. Therefore, it is important to detect both of these protozoan species in water and to find a cost-effective technique for inactivation or management control. This review article summarizes the available detection methods in water and wastewater matrices along with feasible disinfection techniques.展开更多
In recent years, the problem of environmental pollution caused by microplastics has attracted widespread attention. This paper reviews the latest research progress in terms of the source, content and distribution char...In recent years, the problem of environmental pollution caused by microplastics has attracted widespread attention. This paper reviews the latest research progress in terms of the source, content and distribution characteristics, harm, and detection technology of soil microplastics by referring to the relevant literature on soil microplastics worldwide. It concludes that:(1) Existing studies worldwide have detected the presence of microplastics in soil, water, and atmosphere, and the use of agricultural films, sewage sludge,and other man-made activities are the main sources of microplastics in soil;(2) microplastics can adsorb heavy metals, persistent organic pollutants and antibiotics in soil, change the physical and chemical properties of soil. This will result in composite pollution and harm to the ecosystem;(3) microplastics in soil not only can destroy the activity of key soil microorganisms, but also enter the body of crops and soil animals, affecting normal growth of crops and soil animals, and further threaten human health;(4) at present, there is no unified operating standard for the sampling, processing, and detection process of microplastics. Analysis methods such as visual inspection, spectroscopy, and thermal analysis have both advantages and disadvantages, and emerging detection technologies require urgent development.Microplastics have become a new pollutant in soil and their distribution characteristics are closely related to human activities. They pollute the environment and threaten human health through the food chain.Although related research on soil microplastics has just begun, it will become the focus of research in the future.展开更多
Viruses present in water might be harmful for human health and life. Nowadays over 100 pathogenic human virus species occur in water polluted with sewage. Chlorination, which is the most popular disinfection method is...Viruses present in water might be harmful for human health and life. Nowadays over 100 pathogenic human virus species occur in water polluted with sewage. Chlorination, which is the most popular disinfection method is not able to remove easily viruses from treated water. Due to this, it’s necessary to detect viruses in water before treatment in order to determine disinfectant dose and to ensure the sanitary safety level of treated water. The aim of this article is to review viruses detection methods as well as the problems related to implementation of those methods in analysis of water and wastewater samples.展开更多
Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to es...Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.展开更多
Railway Point System(RPS)is an important infrastructure in railway industry and its faults may have significant impacts on the safety and efficiency of train operations.For the fault diagnosis of RPS,most existing met...Railway Point System(RPS)is an important infrastructure in railway industry and its faults may have significant impacts on the safety and efficiency of train operations.For the fault diagnosis of RPS,most existing methods assume that sufficient samples of each failure mode are available,which may be unrealistic,especially for those modes of low occurrence frequency but with high risk.To address this issue,this work proposes a novel fault diagnosis method that only requires the power signals generated under normal RPS operations in the training stage.Specifically,the failure modes of RPS are distinguished through constructing a reasoning diagram,whose nodes are either binary logic problems or those that can be decomposed into the problems of the binary logic.Then,an unsupervised method for the signal segmentation and a fault detection method are combined to make decisions for each binary logic problem.Based on the results of decisions,the diagnostic rules are established to identify the failure modes.Finally,the data collected from multiple real-world RPSs are used for validation and the results demonstrate that the proposed method outperforms the benchmark in identifying the faults of RPSs.展开更多
文摘Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction of bridge pile foundations,accidents such as hole collapse,slurry leakage,and drill sticking will easily occur.In this paper,the principle and method of sonar detection for detecting karst caves at the bottom of bridge piles was introduced,and the sonar detection data and the cave situation at the bottom of the pile during the construction process in combination with the case of Yunnan Zhenguo Highway Project was analyzed,which verifies the practicability and reliability of sonar detection method reliability.
基金The National Natural Science Foundation of China under contract No.41976002。
文摘SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative probabilities between the number of anticyclonic/cyclonic ME fronts(AEF/CEF)and the number of anticyclones/cyclones reached 20%.The northeastern and southwestern parts of these anticyclones had more fronts than the northwestern and southeastern parts,although CEFs were nearly equally distributed in all directions.The number of ME fronts had remarkable seasonal variations,while the eddy kinetic energy(EKE)showed no seasonal variations.The total EKE at the ME fronts was three times of that within the MEs,and it was much stronger in AEFs than in CEFs.The interannual variability in the number of ME fronts and EKE had no significant correlation with the El Ni?o-Southern Oscillation(ENSO)index.Possible mechanisms of ME fronts were discussed,but the contributions of mesoscale eddies to SST fronts need to be quantified in future studies.
基金This work was supported by the National Natural Science Foundation of China No.61672101the Beijing Key Laboratory of Internet Culture and Digital Dissemination Research(ICDDXN004)Key Lab of Information Network Security,Ministry of Public Security,No.C18601.
文摘To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entropy of content name of interest packet.On the one hand,taking advantage of the characteristics of self-similarity is very sensitive to traffic changes,calculating the Hurst index of the traffic,to identify initial IFA attacks.On the other hand,according to the randomness of user requests,calculating the information entropy of content name of the interest packets,to detect the severity of the IFA attack,is.Finally,based on the above two aspects,we use the bilateral detection method based on non-parametric CUSUM algorithm to judge the possible attack behavior in CCN.The experimental results show that flooding attack detection method proposed for CCN can not only detect the attack behavior at the early stage of attack in CCN,but also is more accurate and effective than other methods.
基金supported by the Scientific Research Fund of the Shenzhen International cooperation projects under Grant Nos.(GJHZ20190819151403615)the Natural Science Youth Foundation of China(61801307).
文摘Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems are essentially required due to lack of effective vaccine against dengue virus and the progressive spread of the dengue virus infection.The recent progress in developing micro-and nano-fabrication techniques has led to low cost and scale down the biomedical point-of-care devices.Starting from the conventional and modern available methods for the diagnosis of dengue infection,this review examines several emerging rapid and point-of-care diagnostic devices that hold significant potential for the progress in smart diagnosis tools.The given review revealed that an effective vaccine is required urgently against all the dengue virus serotypes.However,the rapid detection methods of dengue virus help in early treatment and significantly reduce the dengue virus outbreak.
文摘Mycotoxins are widely distributed in various feed ingredients and animal feed. Feed contamination by mycotoxin leads to deteriorated feed,various diseases of animals and even acute death,bringing great loss to breeding industry. The article summarized the harm of mycotoxin to animal and detection methods in feed and animal,in order to provide a reference for further improving mycotoxin detection methods and reducing the harm of mycotoxin to breeding industry.
基金The 940 Hospital COVID-19 Emergency Medical Research Proj ect(No.20yjky020)Scientific Research Foundation of Huoshenshan Hospital(No.HSS-217)。
文摘The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomatic infections is increasing as COVID-19 research continues and SARS-CoV-2 testing improves.Many asymptomatic COVID-19 patients with no obvious clinical symptoms are able to transmit the virus to others due to the presence of SARS-CoV-2 in their bodies,thus greatly accelerating the worldwide pandemic and posing great difficulty and threat to global epidemic prevention and control.Therefore,early detection and identification of asymptomatic infected persons through various testing methods are effective measures to control the COVID-19 pandemic.This article will provide a brief review of the available methods and techniques for detecting asymptomatic COVID-19 patients.
基金Major Science and Technology Innovation Project of Shandong Province(2019JZZy 020609-03).
文摘Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great harm to the breeding industry.In this paper,the harm of mycotoxin to animals and its detection methods in feed and animals are summarized and analyzed,so as to provide the reference basis for further improving the detection methods of mycotoxin and reducing the harm of mycotoxin to breeding industry.
基金Tangshan Science and Technology Planning Project(20150210C)Hebei Provincial Phase II Modern Agricultural Industry Technology System Innovation Team Building Project(HBCT2018120207,HBCT2018160403).
文摘In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.
文摘 Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.……
基金Shenzhen High‐level Hospital Construction Fund,Grant/Award Number:G2022091National Key Research and Development Program of China,Grant/Award Numbers:2022YFC2304401,2022YFC2304402+2 种基金The Special Funds for Strategic Emerging Industry of Shenzhen,Grant/Award Number:F‐2022‐Z99‐502266Shenzhen Science and Technology Program,Grant/Award Number:RCBS20221008093104016Basic and Applied Basic Research Foundation of Guangdong Province,Grant/Award Number:2019A1515110119。
文摘Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell therapy,a promising branch of adoptive T-cell therapy,has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer.At present,CAR-T cells are predominantly used to treat hematological malignancies,although their application in solid tumors is being readily investigated.Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy,few have evaluated predictors of CAR-T cell therapeutic efficacy.Thus,the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy,with a particular focus on biomarkers and their detection methods.
基金supported by the National Natural Science Foundation of China (No.61876167)the Natural Science Foundation of Zhejiang Province (No.LY20F030017)。
文摘Simultaneous localization and mapping(SLAM) technology is a research hotspot in the field of intelligent mobile robot, and many researchers have developed many classic systems in the past few decades. However, most of the existing SLAM methods assume that the environment of the robot is static, which results in the performance of the system being greatly reduced in the dynamic environment. To solve this problem, a new dynamic object detection method based on point cloud motion analysis is proposed and incorporated into ORB-SLAM2. First, the method is regarded as a preprocessing stage, detecting moving objects in the scene, and then removing the moving objects to enhance the performance of the SLAM system. Experiments performed on a public RGB-D dataset show that the motion cancellation method proposed in this paper can effectively improve the performance of ORB-SLAM2 in a highly dynamic environment.
基金Supported by the National Natural Science Foundation of China(51704327)
文摘Most multiphase flow separation detection methods used commonly in oilfields are low in efficiency and accuracy,and have data delay.An online multiphase flow detection method is proposed based on magnetic resonance technology,and its supporting device has been made and tested in lab and field.The detection technology works in two parts:measure phase holdup in static state and measure flow rate in flowing state.Oil-water ratio is first measured and then gas holdup.The device is composed of a segmented magnet structure and a dual antenna structure for measuring flowing fluid.A highly compact magnetic resonance spectrometer system and intelligent software are developed.Lab experiments and field application show that the online detection system has the following merits:it can measure flow rate and phase holdup only based on magnetic resonance technology;it can detect in-place transient fluid production at high frequency and thus monitor transient fluid production in real time;it can detect oil,gas and water in a full range at high precision,the detection isn’t affected by salinity and emulsification.It is a green,safe and energy-saving system.
基金Supported by Standardization System Research Project of Sichuan Provincial Bureau of Quality Supervision(ZYBZ2013-39)
文摘In order to improve the standardized technical system of quantitative analyses for genetically modified organisms( GMOs) and protect China's bio-safety and reduce ecological risk,we establish a quantitative detection method for the genetically modified( GM) maize MON88017 using real-time fluorescent quantitative PCR. Meanwhile,the method is evaluated by several methodological indicators such as specificity,sensitivity,accuracy and uncertainty of measurement. The results show that the method has strong specificity in analysis of genetically modified maize MON88017. The mean value(1. 54%) repeatedly measured for 29 times with the relative deviation of 2. 7% was close to the real value(1. 50%) and the variation coefficient of the measured value was 0. 1. The tested recovery rate is 100% and the uncertainty of measurement is 0. 096. 5 copies of the MON88017 molecular fragment can be detected at 97. 5% confidence level. Consequently,the quantitative detection method established in this paper for the GM maize MON88017 has fairly high specificity,accuracy and sensitivity and this technology established in this paper can provide good technical support for the safety supervision of genetically modified organisms in China.
文摘<i>Entamoeba histolytica</i> is an anaerobic parasitic protozoan and well known as a human pathogen, while its close relative, <i>Entamoeba dispar</i>, also possesses similar characteristics as an infectious agent. These microorganisms are generally transmitted in fecal-contaminated water. However, <i>E. dispar</i> present in industrial wastewater is also capable of creating biofilms that can cause adverse impacts in piping networks. Therefore, it is important to detect both of these protozoan species in water and to find a cost-effective technique for inactivation or management control. This review article summarizes the available detection methods in water and wastewater matrices along with feasible disinfection techniques.
基金jointly supported by the project of China Geological Survey (DD20211574)Guangdong Geological Exploration and Urban Geology Project (2023–25)Public Welfare Geological Survey Project of Shaanxi Geological Survey Institute (202201)。
文摘In recent years, the problem of environmental pollution caused by microplastics has attracted widespread attention. This paper reviews the latest research progress in terms of the source, content and distribution characteristics, harm, and detection technology of soil microplastics by referring to the relevant literature on soil microplastics worldwide. It concludes that:(1) Existing studies worldwide have detected the presence of microplastics in soil, water, and atmosphere, and the use of agricultural films, sewage sludge,and other man-made activities are the main sources of microplastics in soil;(2) microplastics can adsorb heavy metals, persistent organic pollutants and antibiotics in soil, change the physical and chemical properties of soil. This will result in composite pollution and harm to the ecosystem;(3) microplastics in soil not only can destroy the activity of key soil microorganisms, but also enter the body of crops and soil animals, affecting normal growth of crops and soil animals, and further threaten human health;(4) at present, there is no unified operating standard for the sampling, processing, and detection process of microplastics. Analysis methods such as visual inspection, spectroscopy, and thermal analysis have both advantages and disadvantages, and emerging detection technologies require urgent development.Microplastics have become a new pollutant in soil and their distribution characteristics are closely related to human activities. They pollute the environment and threaten human health through the food chain.Although related research on soil microplastics has just begun, it will become the focus of research in the future.
基金supported by grant BKM/514/RAU-1/2013 t.26 from Silesian University of Technology in Gliwice,Poland.
文摘Viruses present in water might be harmful for human health and life. Nowadays over 100 pathogenic human virus species occur in water polluted with sewage. Chlorination, which is the most popular disinfection method is not able to remove easily viruses from treated water. Due to this, it’s necessary to detect viruses in water before treatment in order to determine disinfectant dose and to ensure the sanitary safety level of treated water. The aim of this article is to review viruses detection methods as well as the problems related to implementation of those methods in analysis of water and wastewater samples.
基金Supported by the Natural Science Foundation of Heilongjiang Province(Topic C2017032)Heilongjiang Province Applied Technology Research and Development Program(Topic GA19B104)the National Key Research and Development Program(Topic 2018YFD0300105)。
文摘Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.
基金supported by National Key R&D Program of China(2022YFB2602203)Talent Fund of Beijing Jiaotong University(2021RC274,I22L00131)National Natural Science Foundation of China(U1934219,52202392,52022010,U22A2046,52172322,62271486,62120106011,52172323)。
文摘Railway Point System(RPS)is an important infrastructure in railway industry and its faults may have significant impacts on the safety and efficiency of train operations.For the fault diagnosis of RPS,most existing methods assume that sufficient samples of each failure mode are available,which may be unrealistic,especially for those modes of low occurrence frequency but with high risk.To address this issue,this work proposes a novel fault diagnosis method that only requires the power signals generated under normal RPS operations in the training stage.Specifically,the failure modes of RPS are distinguished through constructing a reasoning diagram,whose nodes are either binary logic problems or those that can be decomposed into the problems of the binary logic.Then,an unsupervised method for the signal segmentation and a fault detection method are combined to make decisions for each binary logic problem.Based on the results of decisions,the diagnostic rules are established to identify the failure modes.Finally,the data collected from multiple real-world RPSs are used for validation and the results demonstrate that the proposed method outperforms the benchmark in identifying the faults of RPSs.
