Fault diagnosis plays a significant role in conducting condition-based maintenance and health management for gas turbines(GTs) to improve reliability and reduce costs. Various diagnosis methods developed by modeling e...Fault diagnosis plays a significant role in conducting condition-based maintenance and health management for gas turbines(GTs) to improve reliability and reduce costs. Various diagnosis methods developed by modeling engine systems or certain components implement faults detection and diagnosis based on the measurement of systemic parameters deviations. However, these conventional model-based methods are hindered by limitations of inability to handle the nonlinear nature, measurement uncertainty, fault coupling and other implementing problems. Recently, the development of artificial intelligence algorithms has provided an effective solution to the above problems, triggering broad researches for data-driven fault diagnosis methods with better accuracy,dynamic performance, and universality. This paper presents a systematic review of recently proposed intelligent fault diagnosis methods for GT engines, according to the classification of shallow learning methods, deep learning methods and hybrid intelligent methods. Moreover, the principle of typical algorithms, the evolution of enhanced methods, and the assessment of pros and cons are summarized to conclude the present status and look forward to the future in the field of GT fault diagnosis. Possible directions for development in method validation, information fusion, and interpretability of intelligent diagnosis methods are concluded in the end to provide insightful concepts for scholars in related fields.展开更多
This paper introduced the characteristics of Glasserella parasuis from the aspects of pathogen,epidemiology,clinical symptoms and anatomical symptoms,and put forward its clinical and laboratory diagnosis methods.Moreo...This paper introduced the characteristics of Glasserella parasuis from the aspects of pathogen,epidemiology,clinical symptoms and anatomical symptoms,and put forward its clinical and laboratory diagnosis methods.Moreover,the disease was differentiated from similar diseases such as mycoplasmal pneumonia of swine,swine flu infectious pleuropneumonia of swine,swine streptococcosis and porcine reproductive and respiratory syndrome.Finally,the prevention and treatment measures of the disease are proposed.展开更多
This paper introduces the characteristics of swine foot-and-mouth disease from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and labor...This paper introduces the characteristics of swine foot-and-mouth disease from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnostic methods.The disease is distinguished with similar diseases,such as swine pox,porcine vesicular stomatitis,and swine vesicular disease.Finally,the prevention and control measures of the disease are proposed.展开更多
This paper introduced the characteristics of avian leukosis from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnost...This paper introduced the characteristics of avian leukosis from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnostic methods.Besides,the disease is also differentiated from similar diseases of chicken such as Marek's disease,infectious bursal disease and reticuloendothelial hyperplasia.Finally,the prevention and control measures against the disease were proposed.展开更多
Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patie...Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patient with American tegumentary leishmaniasis in Mendoza,Argentina,a non-endemic region.Patient concerns:A 43-year-old male was admitted to a tertiary care hospital in Mendoza,Argentina Republic with a history of progressive nasal discharge,septal perforation,facial pain,and pruritus.Despite treatment for presumed nasal myiasis and vasculitis with granulomatosis,symptoms persisted.Diagnosis:American tegumentary leishmaniasis.Interventions:Intravenous liposomal amphotericin B.Outcomes:Follow-up at 30 days showed no recurrence of symptoms with a remarkable clinical improvement of the nasal lesion.Lessons:This case sheds light on the necessity of accurate identification for timely intervention and the need to recognize the diverse manifestations of American tegumentary leishmaniasis to avoid misdiagnosis.展开更多
Objectives: Dengue virus (DENV) infection is a mosquito-borne disease that stands out as one of the major public health issues and has a wide-ranging geographical distribution throughout the tropics and subtropics. Th...Objectives: Dengue virus (DENV) infection is a mosquito-borne disease that stands out as one of the major public health issues and has a wide-ranging geographical distribution throughout the tropics and subtropics. The general alarming increase in the number of cases over the last two decades can be attributed to an extent by the change in national practices to keeping records and reporting dengue to the Ministries of Health, and WHO. Dengue diagnosis is routinely carried out by detection of dengue virus (DENV) antigen NS1 (Non-structural Antigen 1) and/or anti-DENV IgM/IgG antibodies using enzyme-linked immunosorbent assays (ELISAs) and rapid diagnostic tests (RDTs). This study compared the performance of three RDTs and one ELISA used for dengue diagnosis in southeastern, Nigeria. Design: This study adopted a cross-sectional design that included prospective hospital-based surveillance of cases among febrile participants attending two major health facilities within the southeastern region of Nigeria. In this study, 338 HIV-infected participants from two teaching hospitals in Nigeria’s southeast were systematically tested for Dengue with four methods: NS1 RDT, IgG RDT, IgM RDT, and NS1 ELISA. Their specificities and sensitivities were compared, as well as their level of concordance. Their respective performances were also evaluated using the Receivers Operational curve (ROC). Results: Out of the 338 patients, the dengue prevalence from the four dengue diagnostic methods Dengue virus NS1 ELISA, NS1 RDT, IgM and IgG seropositivity were 8.9%, 0.6%, 5.6%, and 8.0%, respectively. The Dengue IgM RDT test indicated 36.8% sensitivity, 92.8% specificity, the IgG anti-dengue specific test indicated 29.6% sensitivity, 92.9% specificity and the dengue NS1 RDT test indicated 3.3% sensitivity, 99.4% specificity when compared with the Dengue NSI Elisa test method as a reference method. Conclusion: The use of NSI ELISA for DENV diagnosis showed good performance and the RDTs showed, to an extent, reliable results compared with ELISA. However, diagnostic laboratories should be aware of performance variations across tests and the possibilities of cross-reactivity that may affect results.展开更多
A Compound fault signal usually contains multiple characteristic signals and strong confusion noise, which makes it difficult to separate week fault signals from them through conventional ways, such as FFT-based envel...A Compound fault signal usually contains multiple characteristic signals and strong confusion noise, which makes it difficult to separate week fault signals from them through conventional ways, such as FFT-based envelope detection, wavelet transform or empirical mode decomposition individually. In order to realize single channel compound fault diagnosis of bearings and improve the diagnosis accuracy, an improved CICA algorithm named constrained independent component analysis based on the energy method （E-CICA） is proposed. With the approach, the single channel vibration signal is firstly decomposed into several wavelet coefficients by discrete wavelet transform（DWT） method for the purpose of obtaining multichannel signals. Then the envelope signals of the reconstructed wavelet coefficients are selected as the input of E-CICA algorithm, which fulfills the requirements that the number of sensors is greater than or equal to that of the source signals and makes it more suitable to be processed by CICA strategy. The frequency energy ratio（ER） of each wavelet reconstructed signal to the total energy of the given synchronous signal is calculated, and then the synchronous signal with maximum ER value is set as the reference signal accordingly. By this way, the reference signal contains a priori knowledge of fault source signal and the influence on fault signal extraction accuracy which is caused by the initial phase angle and the duty ratio of the reference signal in the traditional CICA algorithm is avoided. Experimental results show that E-CICA algorithm can effectively separate out the outer-race defect and the rollers defect from the single channel compound fault and fulfill the needs of compound fault diagnosis of rolling bearings, and the running time is 0.12% of that of the traditional CICA algorithm and the extraction accuracy is 1.4 times of that of CICA as well. The proposed research provides a new method to separate single channel compound fault signals.展开更多
Based on the research of Particle Swarm Optimization (PSO) learning rate, two learning rates are changed linearly with velocity-formula evolving in order to adjust the proportion of social part and cognitional part; t...Based on the research of Particle Swarm Optimization (PSO) learning rate, two learning rates are changed linearly with velocity-formula evolving in order to adjust the proportion of social part and cognitional part; then the methods are applied to BP neural network training, the convergence rate is heavily accelerated and locally optional solution is avoided. According to actual data of two levels compound-box in vibration lab, signals are analyzed and their characteristic values are abstracted. By applying the trained BP neural networks to compound-box fault diagnosis, it is indicated that the methods are sound effective.展开更多
Based on the statics theory, a novel and feasible twice-suspended-mass method(TSMM) was proposed to deal with the seldom-studied issue of fault diagnosis for damping springs of large vibrating screen(LVS). With the st...Based on the statics theory, a novel and feasible twice-suspended-mass method(TSMM) was proposed to deal with the seldom-studied issue of fault diagnosis for damping springs of large vibrating screen(LVS). With the static balance characteristic of the screen body/surface as well as the deformation compatibility relation of springs considered, static model of the screen surface under a certain load was established to calculate compression deformation of each spring. Accuracy of the model was validated by both an experiment based on the suspended mass method and the properties of the 3D deformation space in a numerical simulation. Furthermore, by adopting the Taylor formula and the control variate method, quantitative relationship between the change of damping spring deformation and the change of spring stiffness, defined as the deformation sensitive coefficient(DSC), was derived mathematically, from which principle of the TSMM for spring fault diagnosis is clarified. In the end, an experiment was carried out and results show that the TSMM is applicable for diagnosing the fault of single spring in a LVS.展开更多
Dear Editor,I am Dr.Jia X from the Department of Ophthalmology,Second Xiangya Hospital,Central South University,Changsha,China.I write to present a rare case report of 9p deletion syndrome with congenital infantile gl...Dear Editor,I am Dr.Jia X from the Department of Ophthalmology,Second Xiangya Hospital,Central South University,Changsha,China.I write to present a rare case report of 9p deletion syndrome with congenital infantile glaucoma in an infant,accompanying with an effective method of both diagnosis and treatment.展开更多
With the vigorous development of innovation and entrepreneurship in China,a large number of managers of innovation and entrepreneurship enterprises are studying for MBA degree.The enrollment scale of the MBA program i...With the vigorous development of innovation and entrepreneurship in China,a large number of managers of innovation and entrepreneurship enterprises are studying for MBA degree.The enrollment scale of the MBA program is expanding.In order to improve the quality of MBA dissertation and promote the sustainable and healthy development of MBA education,based on the author's years of MBA teaching experience and dissertation writing guidance experience,this paper introduces three research methods of dissertation writing skills:case study method,enterprise diagnosis method and survey research method,and analyzes five steps of MBA dissertation writing,which can be used as a reference for MBA(EMBA)students to improve their dissertation writing level.展开更多
Toxoplasmosis is a worldwide intracellular parasite disease of natural focus caused by Toxoplasma gondii,which has become one of the most important zoonoses seriously endangering the livestock industry development and...Toxoplasmosis is a worldwide intracellular parasite disease of natural focus caused by Toxoplasma gondii,which has become one of the most important zoonoses seriously endangering the livestock industry development and socio-economic development in the world.In this paper,advances in the epidemiological characteristics of toxoplasmosis,detection and diagnosis of toxoplasmosis,development of T.gondii vaccines were summarized,which provided scientific basis for the control and treatment of T.gondii infection.展开更多
BACKGROUND Paraganglioma of the urinary bladder(PUB)is a nonepithelial tumor of the bladder that is also known as bladder pheochromocytoma.The incidence of nonfunctional paraganglioma of the urinary bladder is rare in...BACKGROUND Paraganglioma of the urinary bladder(PUB)is a nonepithelial tumor of the bladder that is also known as bladder pheochromocytoma.The incidence of nonfunctional paraganglioma of the urinary bladder is rare in clinical practice.CASE SUMMARY A case of nonfunctional paraganglioma of the urinary bladder diagnosed and treated in our hospital is reported herein.A 2.5 cm×2.1 cm moderate-echo mass protruding into the right anterior wall of the bladder was incidentally found during transvaginal color Doppler ultrasound examination.Based on a contrastenhanced computed tomography scan of the bladder,the right anterior wall of the bladder was considered to present a hemangioma.The patient underwent laparoscopic partial resection of the bladder wall in our hospital,and pathological results showed paraganglioma of the bladder.At present,we have followed up with the patient for half a year,and there has been no recurrence of the tumor.CONCLUSION The imaging patterns of PUB and bladder hemangioma are similar.In clinical practice,the possibility of PUB should be considered for a single wide-based isoechoic mass in the bladder,especially functional PUB may cause some adverse effects during surgery.展开更多
[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] Acco...[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.展开更多
Carassius auratus herpesvirus(CaHV)is a pathogen isolated from crucian carp(Carassius auratus)associated with high mortality.A diagnosis method that can detect the virus at an early stage,specifically and accurately,i...Carassius auratus herpesvirus(CaHV)is a pathogen isolated from crucian carp(Carassius auratus)associated with high mortality.A diagnosis method that can detect the virus at an early stage,specifically and accurately,is an urgent requirement for the prevention of CaHV transmission.In the present study,a droplet digital PCR(ddPCR)method based on the tumor necrosis factor receptor(TNFR)gene was established to detect and quantify CaHV DNA with high specificity and no cross-reactions with other aquatic viruses.Skin mucus samples were collected from infected crucian carp from Day 1–8 after infection,and positive amplification was detected on the first day by ddPCR(0.54 copies/μL),whereas the presence of CaHV was not detected by routine PCR until Day 6.Tissue DNA was then collected from the head kidney of 20 fishes which were injected with CaHV and died during the experiment.The five negative samples checked by routine PCR were detected by ddPCR and real-time PCR(qPCR),respectively.The results showed that the positive detection rate of ddPCR(100%)was higher than that of qPCR(40%).The detection limit of the ddPCR was found to be 0.52 copies/μL,which was much lower than the 50.12 copies/μL determined by qPCR.Overall,ddPCR offers a highly promising diagnosis method for the absolute quantification of CaHV in carrier fish and samples from the skin mucus and head kidney with low viral concentrations.展开更多
Pseudorabies(PR),also called Aujeszky's disease,is a highly infectious disease caused by pseudorabies virus(PRV).Without specific host tropism,PRV can infect a wide variety of mammals,including pig,sheep,cattle,et...Pseudorabies(PR),also called Aujeszky's disease,is a highly infectious disease caused by pseudorabies virus(PRV).Without specific host tropism,PRV can infect a wide variety of mammals,including pig,sheep,cattle,etc.,thereby causing severe clinical symptoms and acute death.PRV was firstly reported in China in 1950s,while outbreaks of emerging PRV variants have been documented in partial regions since 2011,leading to significant economic losses in swine industry.Although scientists have been devoting to the design of diagnostic approaches and the development of vaccines during the past years,PR remains a vital infectious disease widely prevalent in Chinese pig industry.Especially,its potential threat to human health has also attracted the worldwide attention.In this review,we will provide a summary of current understanding of PRV in China,mainly focusing on PRV history,the existing diagnosis methods,PRV prevalence in pig population and other susceptible mammals,molecular characteristics,and the available vaccines against its infection.Additionally,promising agents including traditional Chinese herbal medicines and novel inhibitors that may be employed to treat this viral infection,are also discussed.展开更多
基金financially supported by the National Natural Science Foundation of China (No. 61890921, 61890923, and 52372371)the key projects of Aero Engine and Gas Turbine Basic Science Center (No. P2022-B-V-001-001 and P2022B-V-002-001)。
文摘Fault diagnosis plays a significant role in conducting condition-based maintenance and health management for gas turbines(GTs) to improve reliability and reduce costs. Various diagnosis methods developed by modeling engine systems or certain components implement faults detection and diagnosis based on the measurement of systemic parameters deviations. However, these conventional model-based methods are hindered by limitations of inability to handle the nonlinear nature, measurement uncertainty, fault coupling and other implementing problems. Recently, the development of artificial intelligence algorithms has provided an effective solution to the above problems, triggering broad researches for data-driven fault diagnosis methods with better accuracy,dynamic performance, and universality. This paper presents a systematic review of recently proposed intelligent fault diagnosis methods for GT engines, according to the classification of shallow learning methods, deep learning methods and hybrid intelligent methods. Moreover, the principle of typical algorithms, the evolution of enhanced methods, and the assessment of pros and cons are summarized to conclude the present status and look forward to the future in the field of GT fault diagnosis. Possible directions for development in method validation, information fusion, and interpretability of intelligent diagnosis methods are concluded in the end to provide insightful concepts for scholars in related fields.
基金Supported by Major Special Project of Industry University Research Cooperation in Lu'an City(2020-7)Key Research and Development Program of Anhui Province(202204c06020009)+1 种基金The Natural Science Foundation of Anhui Province(2308085MC102)The Anhui Academy of Agricultural Sciences Platform Project(2024YL016).
文摘This paper introduced the characteristics of Glasserella parasuis from the aspects of pathogen,epidemiology,clinical symptoms and anatomical symptoms,and put forward its clinical and laboratory diagnosis methods.Moreover,the disease was differentiated from similar diseases such as mycoplasmal pneumonia of swine,swine flu infectious pleuropneumonia of swine,swine streptococcosis and porcine reproductive and respiratory syndrome.Finally,the prevention and treatment measures of the disease are proposed.
基金Supported by The Key Research and Development Program of Anhui Province(202204c06020009)The Special Fund for Anhui Agriculture Research System(AHCYJXTX-05-13)The Platform Project of the Anhui Academy of Agricultural Science(2024YL016).
文摘This paper introduces the characteristics of swine foot-and-mouth disease from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnostic methods.The disease is distinguished with similar diseases,such as swine pox,porcine vesicular stomatitis,and swine vesicular disease.Finally,the prevention and control measures of the disease are proposed.
基金Supported by Anhui Province Key Research and Development Plan(202204c06020039)Hefei Comprehensive Experimental Station of National Egg Chicken Industry Technology System(CARS-40-S10)+1 种基金Anhui Province Poultry Industry Technology System(AHCYJSTX-06)Anhui Academy of Agricultural Sciences Platform Project(2023YL013).
文摘This paper introduced the characteristics of avian leukosis from the aspects of pathogen,epidemiology,clinical symptoms,and anatomical symptoms,and puts forward clinical comprehensive diagnosis and laboratory diagnostic methods.Besides,the disease is also differentiated from similar diseases of chicken such as Marek's disease,infectious bursal disease and reticuloendothelial hyperplasia.Finally,the prevention and control measures against the disease were proposed.
基金supported by the Consejo Nacional de Investigaciones Cientıfícas y Técnicas(CONICET)(PIP 2021-2023/11220200102779CO).
文摘Rationale:American tegumentary leishmaniasis comprises cutaneous and mucocutaneous manifestations caused by parasitic infections by various Leishmania species.This report details the clinical interventions for a patient with American tegumentary leishmaniasis in Mendoza,Argentina,a non-endemic region.Patient concerns:A 43-year-old male was admitted to a tertiary care hospital in Mendoza,Argentina Republic with a history of progressive nasal discharge,septal perforation,facial pain,and pruritus.Despite treatment for presumed nasal myiasis and vasculitis with granulomatosis,symptoms persisted.Diagnosis:American tegumentary leishmaniasis.Interventions:Intravenous liposomal amphotericin B.Outcomes:Follow-up at 30 days showed no recurrence of symptoms with a remarkable clinical improvement of the nasal lesion.Lessons:This case sheds light on the necessity of accurate identification for timely intervention and the need to recognize the diverse manifestations of American tegumentary leishmaniasis to avoid misdiagnosis.
文摘Objectives: Dengue virus (DENV) infection is a mosquito-borne disease that stands out as one of the major public health issues and has a wide-ranging geographical distribution throughout the tropics and subtropics. The general alarming increase in the number of cases over the last two decades can be attributed to an extent by the change in national practices to keeping records and reporting dengue to the Ministries of Health, and WHO. Dengue diagnosis is routinely carried out by detection of dengue virus (DENV) antigen NS1 (Non-structural Antigen 1) and/or anti-DENV IgM/IgG antibodies using enzyme-linked immunosorbent assays (ELISAs) and rapid diagnostic tests (RDTs). This study compared the performance of three RDTs and one ELISA used for dengue diagnosis in southeastern, Nigeria. Design: This study adopted a cross-sectional design that included prospective hospital-based surveillance of cases among febrile participants attending two major health facilities within the southeastern region of Nigeria. In this study, 338 HIV-infected participants from two teaching hospitals in Nigeria’s southeast were systematically tested for Dengue with four methods: NS1 RDT, IgG RDT, IgM RDT, and NS1 ELISA. Their specificities and sensitivities were compared, as well as their level of concordance. Their respective performances were also evaluated using the Receivers Operational curve (ROC). Results: Out of the 338 patients, the dengue prevalence from the four dengue diagnostic methods Dengue virus NS1 ELISA, NS1 RDT, IgM and IgG seropositivity were 8.9%, 0.6%, 5.6%, and 8.0%, respectively. The Dengue IgM RDT test indicated 36.8% sensitivity, 92.8% specificity, the IgG anti-dengue specific test indicated 29.6% sensitivity, 92.9% specificity and the dengue NS1 RDT test indicated 3.3% sensitivity, 99.4% specificity when compared with the Dengue NSI Elisa test method as a reference method. Conclusion: The use of NSI ELISA for DENV diagnosis showed good performance and the RDTs showed, to an extent, reliable results compared with ELISA. However, diagnostic laboratories should be aware of performance variations across tests and the possibilities of cross-reactivity that may affect results.
基金Supported by National Natural Science Foundation of China(Grant No.51475034)
文摘A Compound fault signal usually contains multiple characteristic signals and strong confusion noise, which makes it difficult to separate week fault signals from them through conventional ways, such as FFT-based envelope detection, wavelet transform or empirical mode decomposition individually. In order to realize single channel compound fault diagnosis of bearings and improve the diagnosis accuracy, an improved CICA algorithm named constrained independent component analysis based on the energy method （E-CICA） is proposed. With the approach, the single channel vibration signal is firstly decomposed into several wavelet coefficients by discrete wavelet transform（DWT） method for the purpose of obtaining multichannel signals. Then the envelope signals of the reconstructed wavelet coefficients are selected as the input of E-CICA algorithm, which fulfills the requirements that the number of sensors is greater than or equal to that of the source signals and makes it more suitable to be processed by CICA strategy. The frequency energy ratio（ER） of each wavelet reconstructed signal to the total energy of the given synchronous signal is calculated, and then the synchronous signal with maximum ER value is set as the reference signal accordingly. By this way, the reference signal contains a priori knowledge of fault source signal and the influence on fault signal extraction accuracy which is caused by the initial phase angle and the duty ratio of the reference signal in the traditional CICA algorithm is avoided. Experimental results show that E-CICA algorithm can effectively separate out the outer-race defect and the rollers defect from the single channel compound fault and fulfill the needs of compound fault diagnosis of rolling bearings, and the running time is 0.12% of that of the traditional CICA algorithm and the extraction accuracy is 1.4 times of that of CICA as well. The proposed research provides a new method to separate single channel compound fault signals.
基金Supported by National Natural Science Foundation (No.50575214)
文摘Based on the research of Particle Swarm Optimization (PSO) learning rate, two learning rates are changed linearly with velocity-formula evolving in order to adjust the proportion of social part and cognitional part; then the methods are applied to BP neural network training, the convergence rate is heavily accelerated and locally optional solution is avoided. According to actual data of two levels compound-box in vibration lab, signals are analyzed and their characteristic values are abstracted. By applying the trained BP neural networks to compound-box fault diagnosis, it is indicated that the methods are sound effective.
基金Project(20120095110001)supported by the PhD Programs Foundation of Ministry of Education of ChinaProject(51134022,51221462)supported by the National Natural Science Foundation of China+1 种基金Project(CXZZ13_0927)supported by Research and Innovation Program for College Graduates of Jiangsu Province,ChinaProject(2013DXS03)supported by the Fundamental Research Funds for Central Universities of China
文摘Based on the statics theory, a novel and feasible twice-suspended-mass method(TSMM) was proposed to deal with the seldom-studied issue of fault diagnosis for damping springs of large vibrating screen(LVS). With the static balance characteristic of the screen body/surface as well as the deformation compatibility relation of springs considered, static model of the screen surface under a certain load was established to calculate compression deformation of each spring. Accuracy of the model was validated by both an experiment based on the suspended mass method and the properties of the 3D deformation space in a numerical simulation. Furthermore, by adopting the Taylor formula and the control variate method, quantitative relationship between the change of damping spring deformation and the change of spring stiffness, defined as the deformation sensitive coefficient(DSC), was derived mathematically, from which principle of the TSMM for spring fault diagnosis is clarified. In the end, an experiment was carried out and results show that the TSMM is applicable for diagnosing the fault of single spring in a LVS.
基金Supported by the Natural Science Foundation of China(No.81370913)
文摘Dear Editor,I am Dr.Jia X from the Department of Ophthalmology,Second Xiangya Hospital,Central South University,Changsha,China.I write to present a rare case report of 9p deletion syndrome with congenital infantile glaucoma in an infant,accompanying with an effective method of both diagnosis and treatment.
基金the National Social Science Found for the project"Research on the Effectiveness and Synergy of Innovation and Entrepreneurship in Promoting Employment"(Project No.19BJY057).
文摘With the vigorous development of innovation and entrepreneurship in China,a large number of managers of innovation and entrepreneurship enterprises are studying for MBA degree.The enrollment scale of the MBA program is expanding.In order to improve the quality of MBA dissertation and promote the sustainable and healthy development of MBA education,based on the author's years of MBA teaching experience and dissertation writing guidance experience,this paper introduces three research methods of dissertation writing skills:case study method,enterprise diagnosis method and survey research method,and analyzes five steps of MBA dissertation writing,which can be used as a reference for MBA(EMBA)students to improve their dissertation writing level.
基金Supported by Project of Jiaxing Science and Technology Bureau(2013AY21035)
文摘Toxoplasmosis is a worldwide intracellular parasite disease of natural focus caused by Toxoplasma gondii,which has become one of the most important zoonoses seriously endangering the livestock industry development and socio-economic development in the world.In this paper,advances in the epidemiological characteristics of toxoplasmosis,detection and diagnosis of toxoplasmosis,development of T.gondii vaccines were summarized,which provided scientific basis for the control and treatment of T.gondii infection.
文摘BACKGROUND Paraganglioma of the urinary bladder(PUB)is a nonepithelial tumor of the bladder that is also known as bladder pheochromocytoma.The incidence of nonfunctional paraganglioma of the urinary bladder is rare in clinical practice.CASE SUMMARY A case of nonfunctional paraganglioma of the urinary bladder diagnosed and treated in our hospital is reported herein.A 2.5 cm×2.1 cm moderate-echo mass protruding into the right anterior wall of the bladder was incidentally found during transvaginal color Doppler ultrasound examination.Based on a contrastenhanced computed tomography scan of the bladder,the right anterior wall of the bladder was considered to present a hemangioma.The patient underwent laparoscopic partial resection of the bladder wall in our hospital,and pathological results showed paraganglioma of the bladder.At present,we have followed up with the patient for half a year,and there has been no recurrence of the tumor.CONCLUSION The imaging patterns of PUB and bladder hemangioma are similar.In clinical practice,the possibility of PUB should be considered for a single wide-based isoechoic mass in the bladder,especially functional PUB may cause some adverse effects during surgery.
基金supported by Independent Innovation Specific Projects of Shandong Province ( 2008ZHZX1A1103)
文摘[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.
基金financially supported by the Science and Technology Commission of Shanghai Municipality(21ZR1427200).
文摘Carassius auratus herpesvirus(CaHV)is a pathogen isolated from crucian carp(Carassius auratus)associated with high mortality.A diagnosis method that can detect the virus at an early stage,specifically and accurately,is an urgent requirement for the prevention of CaHV transmission.In the present study,a droplet digital PCR(ddPCR)method based on the tumor necrosis factor receptor(TNFR)gene was established to detect and quantify CaHV DNA with high specificity and no cross-reactions with other aquatic viruses.Skin mucus samples were collected from infected crucian carp from Day 1–8 after infection,and positive amplification was detected on the first day by ddPCR(0.54 copies/μL),whereas the presence of CaHV was not detected by routine PCR until Day 6.Tissue DNA was then collected from the head kidney of 20 fishes which were injected with CaHV and died during the experiment.The five negative samples checked by routine PCR were detected by ddPCR and real-time PCR(qPCR),respectively.The results showed that the positive detection rate of ddPCR(100%)was higher than that of qPCR(40%).The detection limit of the ddPCR was found to be 0.52 copies/μL,which was much lower than the 50.12 copies/μL determined by qPCR.Overall,ddPCR offers a highly promising diagnosis method for the absolute quantification of CaHV in carrier fish and samples from the skin mucus and head kidney with low viral concentrations.
基金This work was supported by the General Program of National Natural Science Foundation of China(Grants No.31571432/31802252)the Hunan Provincial Natural Science Foundation of China(Grant No.2015JC3097/2020JJ4041)the Postgraduate Scientific Research Innovation Project of Hunan Province(CX20200659).
文摘Pseudorabies(PR),also called Aujeszky's disease,is a highly infectious disease caused by pseudorabies virus(PRV).Without specific host tropism,PRV can infect a wide variety of mammals,including pig,sheep,cattle,etc.,thereby causing severe clinical symptoms and acute death.PRV was firstly reported in China in 1950s,while outbreaks of emerging PRV variants have been documented in partial regions since 2011,leading to significant economic losses in swine industry.Although scientists have been devoting to the design of diagnostic approaches and the development of vaccines during the past years,PR remains a vital infectious disease widely prevalent in Chinese pig industry.Especially,its potential threat to human health has also attracted the worldwide attention.In this review,we will provide a summary of current understanding of PRV in China,mainly focusing on PRV history,the existing diagnosis methods,PRV prevalence in pig population and other susceptible mammals,molecular characteristics,and the available vaccines against its infection.Additionally,promising agents including traditional Chinese herbal medicines and novel inhibitors that may be employed to treat this viral infection,are also discussed.
