Background: As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of th...Background: As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of the effects of benzoic acid on jejunal digestive physiology, especially the antioxidant capacity and mucosal glucagon-like peptide2(GLP-2) concentrations is lacking.Methods: A total of 20 barrows [Duroc ×(Yorkshire × Landrace)] with an average body weight(BW) of 18.75 ± 0.2 kg were used in a 14-d trial to determine the potential mechanisms of benzoic acid supplementation on the performance,nutrient digestibility and jejunal digestive physiology in young pigs. All pigs were randomly allotted to 1 of 2 diets supplemented with 0 or 5000 mg/kg benzoic acid.Results: Relative to the control, benzoic acid supplementation increased the average daily feed intake(ADFI), and average daily gain(ADG) in young pigs(P 〈 0.05), improved the apparent total tract digestibility of dry matter(DM),crude protein(CP), ether extract(EE), gross energy(GE) and crude ash(P 〈 0.05), and enhanced the activities of trypsin,lipase and amylase in the jejunum(P 〈 0.05). Similarly, relative to the control, supplementing benzoic acid in the diet resulted in a trend to reduce the p H values of the digesta(P = 0.06), decreased crypt depth and increased the villus height to crypt depth ratio(P 〈 0.05) in the jejunum of pigs. Finally, benzoic acid supplementation increased the mR NA expression and concentration of glucagon-like peptide 2 and the activities of glutathione peroxidase and superoxide dismutase in the jejunal mucosa of young pigs(P 〈 0.05).Conclusions: In conclusion, supplementation with 5000 mg/kg benzoic acid improved the performance of young pigs through promoting nutrient digestion, improving jejunal antioxidant capacity, and maintaining the jejunal morphology in young pigs.展开更多
Fermented forages are important feedstuffs. Bacillus subtilis inoculants are often used to improve the value of forage legume fermentation. The present work was conducted to study the effects of replacing soybean meal...Fermented forages are important feedstuffs. Bacillus subtilis inoculants are often used to improve the value of forage legume fermentation. The present work was conducted to study the effects of replacing soybean meal with solid-state fermented alfalfa meal(FAM) with B. subtilis ACCC 01746 on growth performance, serum antioxidant and digestive enzyme activities, and cecal microflora in goose. 300 healthy geese with similar body weights were randomly assigned to six treatment groups with five replicates of 10 geese(five males and five females) each. Geese were fed ad libitum for 35 days. Results showed that the geese fed with 4 and 8% FAM exhibited no significant effects on their final body weights(FBW) and average day gain(ADG)(P〉0.05), whereas 12% or higher FAM caused poor growth of the geese compared with control diet(linear(L): P〈0.05). The average daily feed intake(ADFI)(quadratic(Q): P〈0.05) and feed conversion ratio(FCR)(L: P〈0.05) with 8% or higher supplementation level were higher than those of the control group. The activities of antioxidant enzyme in serum increased, and the level of malondialdehyde(MDA) significantly decreased with increasing dietary FAM level(L: P〈0.05). However, no significant differences were observed at 8% or lower supplementation level for glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD)(P〉0.05) and at 4% for catalase(CAT) supplementation level compared with the control group. All diets containing FAM increased digestive enzyme activities in geese. However, geese fed diets with 12% FAM supplementation showed the highest trypsin activities in pancreas(Q: P〈0.05). Supplementation with 12% or higher FAM significantly increased amylase activities in pancreas(L: P〈0.05) and duodenum(L: P〈0.05) compared with the control group. Significant differences were not observed in total anaerobic bacteria between geese fed with FAM and control diets on day 35(P〉0.05). The numbers of Bifidobacterium and Lactobacillus in the cecum of geese fed with FAM significantly increased(L: P〈0.05), but no significant effects were found with 4 and 8% FAM supplementation levels compared with the control(P〉0.05). By contrast, the coliform counts of cecum decreased with increasing inclusion of FAM, but these counts were significantly reduced in geese fed diets with 12% or higher FAM supplementation level(L: P〈0.05). Collectively, our results indicated that supplementation of the basal geese diet with 8% FAM had no apparent adverse effects on growth performance, serum antioxidant enzyme activities, and digestive parameters and beneficial microbiota.展开更多
The effects of magnetic field intensity, roasting temperature and roasting time on digestion rate and settling performance of bauxite with different iron contents were investigated systematically. The results indicate...The effects of magnetic field intensity, roasting temperature and roasting time on digestion rate and settling performance of bauxite with different iron contents were investigated systematically. The results indicate that such magnetic treatment can profoundly change the microstructure and digestion performance of bauxite. For the two samples carrying different iron contents, phase transformation of the aluminum oxide phase proceeds faster in the high iron bauxite than the low one. The optimal pretreatment conditions of low iron bauxite are roasting temperature 550 ℃ and magnetic field intensity 6 T, while for high iron bauxite are 500 ℃ and 9 T. The digestion rate of alumina can reach 95% and 92% at digestion temperature of 190 ℃ and 250 ℃. The settling performances of roasted ore by intense magnetic field after digestion are enhanced through pretreatment.展开更多
This paper investigates the changes of phase and apparent morphology under the combined effects of an intense magnetic field and temperature field.The effect of different roasting conditions on the digestion performan...This paper investigates the changes of phase and apparent morphology under the combined effects of an intense magnetic field and temperature field.The effect of different roasting conditions on the digestion performance of roasted ore is also studied.The results indicate that roasting pretreatment under high magnetic fields can change the microstructure and digestion performance of bauxite.The Al phase changed faster of high iron bauxite than that of low iron bauxite.The digestion performance of low iron bauxite improved significantly through roasting pretreatment in intense magnetic field. But the digestion performance of bauxite get worse with the iron content in bauxite increase,because the high iron content of bauxite lead the shielding effect of magnetic field and make the crystal structure of bauxite stably,the surface area or low iron bauxite increased obviously during pretreatment.The optimal pretreatment condition of low iron bauxite is roasting temperature of 550℃,roasting time of 60min and magnetic field intensity of 6T,while for the high iron is roasting temperature of 500℃,roasting time of 60min and magnetic field intensity of 9T.展开更多
Tomato plant waste(TPW) was used as the feedstock of a batch anaerobic reactor to evaluate the effect of anaerobic digestion on Ralstonia solanacearum and Phytophthora capsici survival. Batch experiments were carrie...Tomato plant waste(TPW) was used as the feedstock of a batch anaerobic reactor to evaluate the effect of anaerobic digestion on Ralstonia solanacearum and Phytophthora capsici survival. Batch experiments were carried out for TS(total solid) concentrations of 2%, 4% and 6% respectively, at mesophilic(37 ± 1°C) and room(20–25°C) temperatures. Results showed that higher digestion performance was achieved under mesophilic digestion temperature and lower TS concentration conditions. The biogas production ranged from 71 to 416 L/kg VS(volatile solids). The inactivation of anaerobic digestion tended to increase as digestion performance improved. The maximum log copies reduction of R. solanacearum and P. capsici detected by quantitative PCR(polymerase chain reaction) were 3.80 and 4.08 respectively in reactors with 4% TS concentration at mesophilic temperatures. However, both in mesophilic and room temperature conditions, the lowest reduction of R. solanacearum was found in the reactors with 6% TS concentration, which possessed the highest VFA(volatile fatty acid) concentration. These findings indicated that simple accumulation of VFAs failed to restrain R. solanacearum effectively, although the VFAs were considered poisonous. P. capsici was nearly completely dead under all conditions. Based on the digestion performance and the pathogen survival rate, a model was established to evaluate the digestate biosafety.展开更多
基金financially supported by a China Pig Modern Industrial Technology System Grant (CARS-36)
文摘Background: As a organic acid, benzoic acid has become one of the most important alternatives for antibiotics,and its beneficial effect on performance in animals has been proven for a decade. However, knowledge of the effects of benzoic acid on jejunal digestive physiology, especially the antioxidant capacity and mucosal glucagon-like peptide2(GLP-2) concentrations is lacking.Methods: A total of 20 barrows [Duroc ×(Yorkshire × Landrace)] with an average body weight(BW) of 18.75 ± 0.2 kg were used in a 14-d trial to determine the potential mechanisms of benzoic acid supplementation on the performance,nutrient digestibility and jejunal digestive physiology in young pigs. All pigs were randomly allotted to 1 of 2 diets supplemented with 0 or 5000 mg/kg benzoic acid.Results: Relative to the control, benzoic acid supplementation increased the average daily feed intake(ADFI), and average daily gain(ADG) in young pigs(P 〈 0.05), improved the apparent total tract digestibility of dry matter(DM),crude protein(CP), ether extract(EE), gross energy(GE) and crude ash(P 〈 0.05), and enhanced the activities of trypsin,lipase and amylase in the jejunum(P 〈 0.05). Similarly, relative to the control, supplementing benzoic acid in the diet resulted in a trend to reduce the p H values of the digesta(P = 0.06), decreased crypt depth and increased the villus height to crypt depth ratio(P 〈 0.05) in the jejunum of pigs. Finally, benzoic acid supplementation increased the mR NA expression and concentration of glucagon-like peptide 2 and the activities of glutathione peroxidase and superoxide dismutase in the jejunal mucosa of young pigs(P 〈 0.05).Conclusions: In conclusion, supplementation with 5000 mg/kg benzoic acid improved the performance of young pigs through promoting nutrient digestion, improving jejunal antioxidant capacity, and maintaining the jejunal morphology in young pigs.
基金supported by the grant agreement from Henan Provincial Natural Science Fund Committee,China(132102110026)the Gushi Sanmu farming Co.,Ltd.,Henan Province,China
文摘Fermented forages are important feedstuffs. Bacillus subtilis inoculants are often used to improve the value of forage legume fermentation. The present work was conducted to study the effects of replacing soybean meal with solid-state fermented alfalfa meal(FAM) with B. subtilis ACCC 01746 on growth performance, serum antioxidant and digestive enzyme activities, and cecal microflora in goose. 300 healthy geese with similar body weights were randomly assigned to six treatment groups with five replicates of 10 geese(five males and five females) each. Geese were fed ad libitum for 35 days. Results showed that the geese fed with 4 and 8% FAM exhibited no significant effects on their final body weights(FBW) and average day gain(ADG)(P〉0.05), whereas 12% or higher FAM caused poor growth of the geese compared with control diet(linear(L): P〈0.05). The average daily feed intake(ADFI)(quadratic(Q): P〈0.05) and feed conversion ratio(FCR)(L: P〈0.05) with 8% or higher supplementation level were higher than those of the control group. The activities of antioxidant enzyme in serum increased, and the level of malondialdehyde(MDA) significantly decreased with increasing dietary FAM level(L: P〈0.05). However, no significant differences were observed at 8% or lower supplementation level for glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD)(P〉0.05) and at 4% for catalase(CAT) supplementation level compared with the control group. All diets containing FAM increased digestive enzyme activities in geese. However, geese fed diets with 12% FAM supplementation showed the highest trypsin activities in pancreas(Q: P〈0.05). Supplementation with 12% or higher FAM significantly increased amylase activities in pancreas(L: P〈0.05) and duodenum(L: P〈0.05) compared with the control group. Significant differences were not observed in total anaerobic bacteria between geese fed with FAM and control diets on day 35(P〉0.05). The numbers of Bifidobacterium and Lactobacillus in the cecum of geese fed with FAM significantly increased(L: P〈0.05), but no significant effects were found with 4 and 8% FAM supplementation levels compared with the control(P〉0.05). By contrast, the coliform counts of cecum decreased with increasing inclusion of FAM, but these counts were significantly reduced in geese fed diets with 12% or higher FAM supplementation level(L: P〈0.05). Collectively, our results indicated that supplementation of the basal geese diet with 8% FAM had no apparent adverse effects on growth performance, serum antioxidant enzyme activities, and digestive parameters and beneficial microbiota.
基金Projects(U1202274,51004033,51204040,50974035)supported by the National Natural Science Foundation of ChinaProjects(2010AA03A405,2012AA062303)supported by the National High Technology Research and Development Program(863 Prograam)of ChinaProject(N100302005)supported by the Fundamental Research Funds for the Central Universities,China
文摘The effects of magnetic field intensity, roasting temperature and roasting time on digestion rate and settling performance of bauxite with different iron contents were investigated systematically. The results indicate that such magnetic treatment can profoundly change the microstructure and digestion performance of bauxite. For the two samples carrying different iron contents, phase transformation of the aluminum oxide phase proceeds faster in the high iron bauxite than the low one. The optimal pretreatment conditions of low iron bauxite are roasting temperature 550 ℃ and magnetic field intensity 6 T, while for high iron bauxite are 500 ℃ and 9 T. The digestion rate of alumina can reach 95% and 92% at digestion temperature of 190 ℃ and 250 ℃. The settling performances of roasted ore by intense magnetic field after digestion are enhanced through pretreatment.
基金Item Sponsored by the Natural Science Foundation of China(NO.51004033,No.50934005,No.50974035,No.51074047)National 863 plan(2010AA03A405,2012AA061403)+1 种基金Operation expenses for universities'basic scientific research(N100302005)The doctoral fund of EDU gov(20050145029)
文摘This paper investigates the changes of phase and apparent morphology under the combined effects of an intense magnetic field and temperature field.The effect of different roasting conditions on the digestion performance of roasted ore is also studied.The results indicate that roasting pretreatment under high magnetic fields can change the microstructure and digestion performance of bauxite.The Al phase changed faster of high iron bauxite than that of low iron bauxite.The digestion performance of low iron bauxite improved significantly through roasting pretreatment in intense magnetic field. But the digestion performance of bauxite get worse with the iron content in bauxite increase,because the high iron content of bauxite lead the shielding effect of magnetic field and make the crystal structure of bauxite stably,the surface area or low iron bauxite increased obviously during pretreatment.The optimal pretreatment condition of low iron bauxite is roasting temperature of 550℃,roasting time of 60min and magnetic field intensity of 6T,while for the high iron is roasting temperature of 500℃,roasting time of 60min and magnetic field intensity of 9T.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201503135-17)
文摘Tomato plant waste(TPW) was used as the feedstock of a batch anaerobic reactor to evaluate the effect of anaerobic digestion on Ralstonia solanacearum and Phytophthora capsici survival. Batch experiments were carried out for TS(total solid) concentrations of 2%, 4% and 6% respectively, at mesophilic(37 ± 1°C) and room(20–25°C) temperatures. Results showed that higher digestion performance was achieved under mesophilic digestion temperature and lower TS concentration conditions. The biogas production ranged from 71 to 416 L/kg VS(volatile solids). The inactivation of anaerobic digestion tended to increase as digestion performance improved. The maximum log copies reduction of R. solanacearum and P. capsici detected by quantitative PCR(polymerase chain reaction) were 3.80 and 4.08 respectively in reactors with 4% TS concentration at mesophilic temperatures. However, both in mesophilic and room temperature conditions, the lowest reduction of R. solanacearum was found in the reactors with 6% TS concentration, which possessed the highest VFA(volatile fatty acid) concentration. These findings indicated that simple accumulation of VFAs failed to restrain R. solanacearum effectively, although the VFAs were considered poisonous. P. capsici was nearly completely dead under all conditions. Based on the digestion performance and the pathogen survival rate, a model was established to evaluate the digestate biosafety.