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Dynamic culture of a thermosensitive collagen hydrogel as an extracellular matrix improves the construction of tissue-engineered peripheral nerve 被引量:3
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作者 Lanfeng Huang Rui Li +5 位作者 Wanguo Liu Jin Dai Zhenwu Du Xiaonan Wang Jianchao Ma Jinsong Zhao 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第14期1371-1378,共8页
Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydro... Tissue engineering technologies offer new treatment strategies for the repair of peripheral nerve injury, hut cell loss between seeding and adhesion to the scaffold remains inevitable. A thermosensitive collagen hydrogel was used as an extracellular matrix in this study and combined with bone marrow mesenchymal stem cells to construct tissue-engineered peripheral nerve composites in vitro. Dynamic culture was performed at an oscillating frequency of 0.5 Hz and 35° swing angle above and below the horizontal plane. The results demonstrated that bone marrow mesenchymal stem cells formed membrane-like structures around the poly-L-lactic acid scaffolds and exhibited regular alignment on the composite surface. Collagen was used to fill in the pores, and seeded cells adhered onto the poly-L-lactic acid fibers. The DNA content of the bone marrow mesenchymal stem cells was higher in the composites constructed with a thermosensitive collagen hydrogel compared with that in collagen I scaffold controls. The cellular DNA content was also higher in the thermosensitive collagen hydrogel composites constructed with the thermosensitive collagen hydrogel in dynamic culture than that in static culture. These results indicate that tissue-engineered composites formed with thermosensitive collagen hydrogel in dynamic culture can maintain larger numbers of seeded cells by avoiding cell loss during the initial adhe-sion stage. Moreover, seeded cells were distributed throughout the material. 展开更多
关键词 nerve regeneration peripheral nerve biomaterials extracellular matrix TISSUEENGINEERING nerve scaffold bone marrow mesenchymal stem cells thermosensitive collagen hydrogel poly-L-lactic acid dynamic culture NSFC grant neural regeneration
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Chinese Cultural Dynamics and Childhood:Towards a New Individuality for Education
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作者 Guoping ZHAO 《Frontiers of Education in China》 2010年第4期579-595,共17页
This paper explores,through the lens of childhood,the Chinese cultural dynamics that encourage harmonious human relationships at the price of individual development and yet support a deep appreciation of natural human... This paper explores,through the lens of childhood,the Chinese cultural dynamics that encourage harmonious human relationships at the price of individual development and yet support a deep appreciation of natural human experiences that allows room for the development of individuality.The purpose of such investigation is to reexamine our cultural heritage in regard to the ways we conceptualize human beings and treat them,retracing its promises,difficulties,pitfalls,and spiritual resources,and thus to help reconfigure a new individuality for education. 展开更多
关键词 Chinese cultural dynamics CHILDHOOD INDIVIDUALITY cultural revitalization
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