Functional constipation(FC)is a chronic discasc that significantly afcts the life quality of patients.Acupuncture has been used for the treatment of FC for many years,but its effectiveness has not been scientifcally a...Functional constipation(FC)is a chronic discasc that significantly afcts the life quality of patients.Acupuncture has been used for the treatment of FC for many years,but its effectiveness has not been scientifcally assessed.The present study aimed to evaluate the efficacy of electro-acupuncture(EA)in relieving the symptoms,mental states and quality of life(QOL)of FC patients.A total of 96 FC patients were randomly allocated into EA,mosapride&sham EA group(MS)and mosapride control group(MC).In the EA group,patients were treated with 16 sessions of needling at Quchi(LI1I)and Shangjuxu(ST37)bilaterally,5 times a week in the first 2 weeks,and 3 times a.week in the last 2 weeks.In the MC group,patients were treated with 5 mg mosapride citrate three times a day for 4 weeks.In the MS group,patients underwent sham EA and the same mosapride citrate treatment as in the MC group.The primary outcome was the number of weekly spontaneous bowel movements(SBMs).The secondary outcomes included stool consistency,intensity of defecating difficulty,36-Item Short-Form Health Survey(SF-36),Self-rating Anxiety Scale(SAS),Self-rating Depression Scale(SDS),and the validated Patient Assessment of Constipation-Quality of Life(PAC-QOL).The results showed that as compared with the baseline,EA significantly improved the weekly SBMs,stool consistency and intensity of defecating difficulty(P<0.05).It also partly ameliorated the PAC-QOL,SF-36,SDS and SAS scores when compared with MC or MS group(P<0.05).However,no significant difference was observed between MS and MC groups in bowel function outcomes and QOL scores.It was concluded that EA could effectivcly improve bowel function,mental states and QOL of FC patients.展开更多
BACKGROUND:Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However,there are few reports on the influence...BACKGROUND:Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However,there are few reports on the influence of electro-acupuncture at the Du meridian on neural stem cell proliferation. OBJECTIVE:To observe the influence of electro-acupuncture at Ren and Du meridians on neural stem cell proliferation in the subventricular zone and altered signal transduction in cerebral ischemia rats. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Human Anatomy,Medical College of Sun Yat-sen University from May 2006 to February 2008. MATERIALS:Mouse anti-rat bromodeoxyuridine (BrdU) monoclonal antibody was provided by Sigma,USA; mouse anti-rat nestin monoclonal antibody and extracellular signal-regulated protein kinase (ERK) specific inhibitor PD98059 were provided by Calbiochem,Germany; acupuncture needle was provided by Suzhou Acupuncture Supplies,China. METHODS:A total of 126 rats were randomly assigned to four groups:model (n = 36),Du meridian (n = 36),Ren/Du meridian (n = 36),and Ren/Du meridian + PD98059 (n = 18). Rats in the Ren /Du meridian + PD98059 group were observed on days 7 (n = 6) and 14 (n = 12) after cerebral ischemia injury. Rats in the model,Du meridian,and Ren/Du meridian groups were observed on days 7,14,and 28 after cerebral ischemia injury,with 12 rats per group at each time point. Thread occlusion was used to establish middle cerebral artery occlusion models. Electro-acupuncture was performed at Renzhong (DU 26) and Baihui (DU 20) acupoints in the Du meridian group,as well as Chengjiang (RN 24),Guanyuan (RN 4),Renzhong,and Baihui acupoints in the Ren/Du meridian and Ren/Du meridian + PD98059 groups 2 days after model establishment. In addition,electro-acupuncture stimulation with disperse-dense waves was performed,with 30 Hz disperse wave,100 Hz dense wave,and 5 V intensity for 20 minutes. Rats in the Ren/Du meridian + PD98059 group were treated with 0.2 μg PD98059 injection into the subventricular zone,2 μL per rat. Rats in the model group were not treated with electro-acupuncture. MAIN OUTCOME MEASURES:BrdU/nestin immunofluorescent staining was used to detect proliferating neural stem cells in the subventricular zone of cerebral ischemia rats; Western blot was used to determine phosphorylated ERK1 and 2 (pERK1/2) expression in the subventricular zone. RESULTS:On days 14 and 28 after cerebral ischemia,there were significantly more BrdU-positive and BrdU/nestin-positive cells in the Ren /Du meridian group compared with the Du meridian group (P < 0.05). PD98059 decreased the number of BrdU-positive and BrdU/nestin-positive cells induced by electro-acupuncture at the Ren and Du meridians (P < 0.05). On days 7,14,and 28 after treatment,pERK1/2 expression was significantly greater in the Du meridian and Ren/Du meridian groups compared with the model group (P < 0.05). The promoting effect of electro-acupuncture at Ren and Du meridians on ERK1/2 phosphorylation was superior to electro-acupuncture at the Du meridian alone on day 14 after model induction (P < 0.05). However,PD98059 completely abolished the promoting effect of electro-acupuncture at Ren/Du meridians on pERK1/2 expression (P < 0.05). CONCLUSION:Electro-acupuncture at Ren and Du meridians increased proliferation of subventricular zone neural stem cells,which was related to activation of the ERK pathway in a rat model of cerebral ischemia injury.展开更多
This study examined the effect of electro-acupuncture (EA) combined with transcranial magnetic stimulation (TMS) therapy at different time windows on learning and memory ability of rats with cerebral infarction and th...This study examined the effect of electro-acupuncture (EA) combined with transcranial magnetic stimulation (TMS) therapy at different time windows on learning and memory ability of rats with cerebral infarction and the underlying mechanism.Two hundred SD rats were randomly divided into four groups:normal group,sham-operated group,model group and EA+TMS group,and each group was then divided into five sub-groups in terms of the different time to start treatment post operation:6,12,24,48 and 72 h.Cerebral infarction models were established in the model and the EA+TMS groups by left middle cerebral artery occlusion/reperfusion (MCAO/R).After treatment for 14 d,the Morris water maze test was applied to examine the spatial learning and memory abilities of rats.In infarcted area,the expression of caspase-3 was immunohistochemically detected,and real-time fluorescent quantitative PCR was used to measure the expression of Bcl-2 mRNA.The results showed that in EA+TMS group compared with model group at the same treatment time windows,the escape latency was substantially shortened,the expression of caspase-3 was considerably decreased and the expression level of Bcl-2 mRNA significantly increased (P<0.05).In the EA+TMS sub-groups,the escape latency was shortest,the expression level of caspase-3 lowest,and the expression level of Bcl-2 mRNA highest at the treatment time window of 24 h.It was concluded that EA combined with TMS can promote neurological function of rats with cerebral infarction by increasing the expression level of Bcl-2 mRNA and decreasing the expression of caspase-3.The best time window is 24 h after perfusion treatment to ischemia.展开更多
AIM To observe whether there are differences in the effects of electro-acupuncture(EA) and moxibustion(Mox) in rats with visceral hypersensitivity. METHODS EA at 1 m A and 3 m A and Mox at 43?℃ and 46?℃ were applied...AIM To observe whether there are differences in the effects of electro-acupuncture(EA) and moxibustion(Mox) in rats with visceral hypersensitivity. METHODS EA at 1 m A and 3 m A and Mox at 43?℃ and 46?℃ were applied to the Shangjuxu(ST37, bilateral) acupoints in model rats with visceral hypersensitivity. Responses of wide dynamic range neurons in dorsal horns of the spinal cord were observed through the extracellular recordings. Mast cells(MC) activity in the colons of rats were assessed, and 5-hydroxytryptamine(5-HT), 5-hydroxytryptamine 3 receptor(5-HT3R) and 5-HT4Rexpressions in the colons were measured.RESULTS Compared with normal control group, responses of wide dynamic range neurons in the dorsal horn of the spinal cord were increased in the EA at 1 m A and 3 m A groups(1 m A: 0.84 ± 0.74 vs 2.73 ± 0.65, P < 0.001; 3 m A: 1.91 ± 1.48 vs 6.44 ± 1.26, P < 0.001) and Mox at 43?℃ and 46?℃ groups(43?℃: 1.76 ± 0.81 vs 4.14 ± 1.83, P = 0.001; 46?℃: 5.19 ± 2.03 vs 7.91 ± 2.27, P = 0.01). MC degranulation rates and the expression of 5-HT, 5-HT3 R and 5-HT4 R in the colon of Mox 46?℃ group were decreased compared with model group(MC degranulation rates: 0.47 ± 0.56 vs 0.28 ± 0.78, P < 0.001; 5-HT: 1.42 ± 0.65 vs 7.38 ± 1.12, P < 0.001; 5-HT3R: 6.62 ± 0.77 vs 2.86 ± 0.88, P < 0.001; 5-HT4R: 4.62 ± 0.65 vs 2.22 ± 0.97, P < 0.001). CONCLUSION The analgesic effects of Mox at 46?℃ are greater than those of Mox at 43?℃, EA 1 m A and EA 3 m A.展开更多
BACKGROUND: Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen,pancreas,intestines,and nervous system in modern medicine.OBJECTIVE: To test whether electro-acupuncture could ...BACKGROUND: Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen,pancreas,intestines,and nervous system in modern medicine.OBJECTIVE: To test whether electro-acupuncture could alter basic fibroblast growth factor (bFGF) protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.DESIGN,TIME AND SETTING: The randomized,controlled,in vivo animal experiment was performed at the National Level-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine,between March and November in 2008.MATERIALS: Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co.Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical.Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies,China.Huatuo Brand electroacupuncture instrument (type SDZ-II) was purchased from Suzhou Medical Appliance Factory,China.METHODS: A total of 96 male Sprague Dawley rats were randomly assigned to control (n = 32) and induction (n = 64) groups.Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb.The successful models were randomized into two groups: model and electro-acupuncture,with 32 rats in each group.Elec-tro-acupuncture was administered at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints using a condensation wave and rarefaction (condensation wave 15 Hz) at a strength of 6–15 V for 20 minutes,once per day.The appearance of a slight shiver in the corresponding locus was taken as the standard.According to electro-acupuncture time points,each group was assigned to four subgroups at 7,14,28,and 49 days,respectively,with eight rats in each subgroup.Immunohistochemical staining,image analysis,and reverse-transcription polymerase chain reaction were performed at different time points.MAIN OUTCOME MEASURES: bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.RESULTS: After 7 days of electro-acupuncture therapy,bFGF protein and mRNA expression significantly increased compared with the model and control groups (P < 0.05).After 14 days,bFGF protein and mRNA expression decreased until 28 days,where levels were then equal to the model group and greater than the control group (P < 0.05).After 49 days,the above indices remained increased in the electro-acupuncture group compared to the model and control groups (P < 0.05).CONCLUSION: Continuous electro-acupuncture maintained a high level of bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.展开更多
The influence of electro-acupuncture at Neiguan (PC 6)and five shu points of twelve regular meridians at different time on systolic time interval (ST I) of normal young adultswere studied with 6×6 Latin square de...The influence of electro-acupuncture at Neiguan (PC 6)and five shu points of twelve regular meridians at different time on systolic time interval (ST I) of normal young adultswere studied with 6×6 Latin square design in this work.In 12 groups according to 12 regular merldlans,the STIs were measured respectively just before and after electro-acupuncture on Neiguan andfive shu points at 00:00,04:00,08:00,12:00,16:00 and 20:00 of a day.It was found thatexcept the PEPI and PEPI/LVETI ratio among Shenmen(HT 7),Shaochong(HT 9)and Neiguan,Guanchong(TE)and Yemen(TE 2)points,the effects of electro-acupuncture at Neiguan pointand five shu points of twelve regular meridians on the indices of STI of normal young adults wereshowing no slgnificant differences.There also was no difference in different sex.But the indices ofSTI were changed when electroacupuncture on the different points of different meridians at differenttime.展开更多
Objective:To summarize the current clinical evidence related to the therapeutic effects and safety of adjuvant scalp electro-acupuncture (SEA) treatment for Parkinson's disease in China.Methods:Following the PRISM...Objective:To summarize the current clinical evidence related to the therapeutic effects and safety of adjuvant scalp electro-acupuncture (SEA) treatment for Parkinson's disease in China.Methods:Following the PRISMA statement,seven electronic databases were searched to retrieve randomized controlled clinical trials that used SEA combined with medication as the treatment intervention,and medication as the control.RevMan 5.3 was used to analyze outcomes,including the Unified Parkinson's Disease Rating Scale (UPDRS),Webster scale,effectiveness rate,and UPDRS III.Results:Nine randomized controlled trials,with certain methodological flaws and risks of bias,were included that involved 474 participants.SEA combined with medication was more effective than medication alone in overall therapeutic effects,as evidenced by total UPDRS scores (mean difference (MD):7.15,95% confidence interval [CI] 0.24 to 14.07,P =.04),Webster scores (MD:1.60,95% CI 0.20 to 2.99,P =.03),and effectiveness rate (risk ratio:1.35,95% CI 1.19 to 1.54,P <.001).In addition,there was significant improvement in pooled motor function results after adjuvant SEA treatment compared with medication alone (MD:5.75,95% Cl 4.18 to 7.32,P <.001).Conclusion:The combination of SEA and medication may be a promising intervention for patients with Parkinson's disease,especially to improve motor function.However,results were inconclusive,and additional studies with rigorous experimental design and larger sample sizes are needed to verify these results.展开更多
Objective:To determine whether electro-acupuncture (EA) therapy could improve the cognitive functions of amyloid precursor protein Swedish mutation (APPswe)/presenilin 1 deleted in exon 9 (PS1dE9) mice and examine whe...Objective:To determine whether electro-acupuncture (EA) therapy could improve the cognitive functions of amyloid precursor protein Swedish mutation (APPswe)/presenilin 1 deleted in exon 9 (PS1dE9) mice and examine whether EA treatment could attenuate neuroinflammation by targeting the toll-like receptor 4 (TLR4)/myeloid differentiation primary response factor 88 (MyD88) signaling pathway.Methods:Twenty-seven double transgenic APPswe/PS1dE9 mice were randomly allocated into three groups:an Alzheimer's disease model group (AD group),a medication group (M group) and an EA treatment group (EA group).Each group contained nine mice,and nine wild-type mice were used in a normal group (N group).The animals in the M group were treated with oral administrations of 0.92 mg/kg donepezil hydrochloride for 15 days.For animals in the EA group,EA treatments were used on the Yintang (GV 29) and Baihui (GV 20) acupoints for 20 minutes,and the Shuigou (GV 26) acupoint was pricked without needle retention following EA treatments.Following treatments,the spatial learning and memory of the mice were measured using the Morris water maze test.The expression levels of TLR4,MyD88,nuclear factor kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) were analyzed by immunohistochemical staining and western blot.Results:The escape latencies of the M and EA groups were significantly lower than those of the AD group (vs M,P =.002;vs EA,P <.001).Moreover,compared with the AD group,the numbers of platform crossings was higher (vs M,P =.038;vs EA,P =.008) and the latency time for target quadrants was longer (vs M,P =.002;vs EA,P =.001) in the M and EA groups (P <.05).Furthermore,in the M and EA groups,the expression levels of TLR4,MyD88,NF-κB and iNOS decreased significantly compared with those of the AD group (all P <.01).Conclusion:EA treatment enhanced the memory and learning abilities of APPswe/PS1dE9 mice by regulating the TLR4/MyD88 inflammatory signaling pathway.展开更多
OBJECTIVE: To investigate that whether electro-acupuncture(EA) precondition can reduce the incidence of postoperative cognitive dysfunction(POCD) following knee replacement and its safety in elderly. METHODS: A total ...OBJECTIVE: To investigate that whether electro-acupuncture(EA) precondition can reduce the incidence of postoperative cognitive dysfunction(POCD) following knee replacement and its safety in elderly. METHODS: A total of 60 participants met the inclusion criteria were enrolled in a randomized controlled trial with the ratio of 1:1, with 30 cases in the treatment group and 30 cases in the control group. The participants in the treatment group were provided with realEA therapy whereas participants in control group were provided with placebo-EA therapy. Interventions were offered 5 days prior to the surgery, once daily, and for a total of 5 days. The scores of Mini-Mental State Examination(MMSE), and contents of serumal inflammatory cytokines including interleukin 1β(IL-1β) and tumor necrosis factor-α(TNF-α) were observed at 24 hours prior-and posterior-to the surgery respectively for assessing the incidence of POCD among patients. Meanwhile, adverse effects were monitored and recorded. RESULTS:(1) After surgery, both treatment group and control group showed a significant decrease in MMSE global scores(P < 0.001, < 0.001, respectively), and the score in control group decreased more significantly than that in treatment group(P < 0.05);(2) Contents of serumal IL-1β and TNF-α were significantly increased in both groups after 24 hours posterior to the surgery(P < 0.001), and the contents in control group increased more significantly than that in treatment group(P < 0.001);(3) After surgery, the incidence of POCD was 20% in treatment group versus 36.67% in control group. There was no statistical difference between 2 groups(P > 0.05);(4) No serious adverse events were reported in this trial, except 1 patient from treatment group had a slight hematoma after receiving acupuncture. CONCLUSION: EA precondition might reduce cognitive impairments after 24 hours posterior to knee replacement surgery in elderly through inhibiting expression of inflammatory cytokines, including both IL-1β and TNF-α. However, there is insufficient evidence to support that EA precondition could reduce incidence of POCD.展开更多
Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar...Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.展开更多
44 cats were used in this experiment. The spinal cords of 40 animals were injuredat about L<sub>1</sub> level by using Allen’s method （400g/cm） and randomly divided into 2 groups: A)electro-acupuncture ...44 cats were used in this experiment. The spinal cords of 40 animals were injuredat about L<sub>1</sub> level by using Allen’s method （400g/cm） and randomly divided into 2 groups: A)electro-acupuncture treatment group （n=20） and B) control group（n=20）. 2 weeks after spinalcord injury, 80% of animals in the treatment group were survival and 45% of animals in the con-trol group were survival. There was significant difference between these two groups （p【0. 05）.The motor evoked potential was recorded two weeks after spinal cord injury. The latency of theshort latency peak was 15. 61±4. 98 mS and the amplitude was 10. 61 0. 59 mV in the treatmentgroup; and 21. 7±5. 02 mS and 0. 56±0. 32 mV in the control group. Both latency and amplitudewere significant different between the two groups （P【0. 05, P【0. 05）. The Anti-NF-H （neuro-filament） was determined at 2 and 4 weeks respectively after spinal cord injury. The more NF-Hpositive labeling fibers were found in the treatment group than in the control group （P【0. 01 ）.展开更多
In this study, in situ hybridization histochemistry technique was used to observe the effect of electro-acupuncture (EA) on glutamate transporter mRNA expression following focal cerebral ischemia. We observed that glu...In this study, in situ hybridization histochemistry technique was used to observe the effect of electro-acupuncture (EA) on glutamate transporter mRNA expression following focal cerebral ischemia. We observed that glutamate transporter mRNA expression decreased significantly in the area penumbra of cerebral cortex at 12h following focal cerebral ischemia. EA did not change glutamate transporter mRNA expression in normal rat brain but prevented the decrease of glutamate transporter mRNA expression in the area penumbra of cerebral cortex following focal cerebral ischemia. The results suggest that the protective effect of EA on ischemic neuronal death may bhe related to the increase of uptake of glutamate by its transporter.展开更多
Rett syndrome is a progressive neurodevelopmental disorder that lacks effective treatments.Although deep-brain stimulation can alleviate some symptoms in Rett model mice,this interventional manipula-tion requires deli...Rett syndrome is a progressive neurodevelopmental disorder that lacks effective treatments.Although deep-brain stimulation can alleviate some symptoms in Rett model mice,this interventional manipula-tion requires deliberate surgical operations.Here,we report that electro-acupuncture stimulation(EAS)can ameliorate symptoms of an Mecp2-knockout rat model of Rett syndrome from the remote acupoints Baihui(GV 20),Yongquan(KI 1),and Shenmen(HT 7).We find that EAS not only prolongs the survival time of Rett rats,but also improves their behavior ability,including locomotion,motor coordination,and social interaction.Neural activation was observed in the substantia nigra of the midbrain,corpus striatum,and cerebral cortex of wild-type and Rett model rats,as reflected by the increased expression of the c-Fos protein.Hence,EAS provides a potential promising therapeutic tool for treating neurodevel-opmental diseases.展开更多
119 patients with gastroduodenal diseases were observed,clinically and experi-mentally,on the effect of electro-acupuncture at the auricular acupoint"Gastric area" on gastrogram,and the effect was compared w...119 patients with gastroduodenal diseases were observed,clinically and experi-mentally,on the effect of electro-acupuncture at the auricular acupoint"Gastric area" on gastrogram,and the effect was compared with that of Heart area and Ear tip points.There was a significant differ-ence in the amplitudes and frequencies of gastrogram of patients between pre-and post-acupuncture(P【0.01),with good diphasic regulation;the effect of acupuncturing at"Gastric area" is the mostsignificant as compared with that at "Heart" and "Ear tip".The experiment demonstrates that thereis a relationship between the "Gastric area" and stomach,and indicates that there is relative specificityof "Gastric Area" to stomach.This provides the experimental foundation for the auricular acupointtherapy in clinic.展开更多
Electro- acupuncture (EA) applied to acupoints of GV channel suppresses spike- wave discharges(SW) of PTZ-induced petit mal epilepsy in rats. EA decreases cortical excitability and increases its inhibitory process. El...Electro- acupuncture (EA) applied to acupoints of GV channel suppresses spike- wave discharges(SW) of PTZ-induced petit mal epilepsy in rats. EA decreases cortical excitability and increases its inhibitory process. Electric stimulation of subcortical nuclei: caudate n., cuneiform n. of midbrain RF, substantia nigra and superior colliculus of rats with petit mal epilepsy can suppress SW, and EA effect becomes low after destruction of the latter at two sites. Stimulation and destruction applied to reticular n. of thalamus elicit reverse effect. Microinjection of drugs affecting neurotransmitters: HC3, haloperidal, PCA and 3-MP into certain parts of brain leads to prolonged SW and reduced EA effect. Injection of muscimol shortens SW duration and reinforces EA effect, while baclofen induces reverse result. AP5 causes shortening of SW duration. The contents of GABA, glycine and taurine are increased markedly in sensory-motor area and optic area of the cortex after EA, while glutamate is decreased. The GABA contents of thalamic reticular n. and reticular part of substantia nigra are also obviously elevated.展开更多
Nineteen patients suffering from common rnental diseases were treated by computer-ized electrode and electro-acupuncture. lt was revealed that 66. 7% of 9 cases treated by electrodewere rnarkedly effective without sig...Nineteen patients suffering from common rnental diseases were treated by computer-ized electrode and electro-acupuncture. lt was revealed that 66. 7% of 9 cases treated by electrodewere rnarkedly effective without significant difference to the effective rate of 1O cases treated throughelectro-acupuncture (P】0. 05). The score of self anxiety scale in the patients treated by electrodewas markedly reduced, while the score of sleep state scale was markedly decreased in those patientstreated by electro-acupuncture. There was no side-effect in the surface electrode therapy l the patientswere without fear, and accepted it with m0re pleasure.展开更多
Electrochemical therapy, ECT, has been used to treat 122 cases of various kinds of tu-mour, among them, 81 cases were malignant tumours (32 cases facial, 18 cases subaural, 16 cases labial, 15 cases tongue), and the o...Electrochemical therapy, ECT, has been used to treat 122 cases of various kinds of tu-mour, among them, 81 cases were malignant tumours (32 cases facial, 18 cases subaural, 16 cases labial, 15 cases tongue), and the other 41 cases were bening tumours (cavermous hemangioma at facial region 23 cases and in oral cavity 18 cases). Pathological examination of malignant tumours revealed that there were 63 cases of squamous epithelial cancers and 18 cases of adenocarcinoma. Cavermous he-mangioma is benign tumour and congenital. Special designed and made platinum needles were inserted into the tumours and the needles were connected to an instrument protucing a direct current for treat-ment. Voltage used was usually 8 volts with current 60 - 80 mA. The quantity elecricity applied was 100 coulombs per 1 cm in diameter of the tumour. The effectiveness of treating 81 cases malignant tu-mours was: CR 42 cases (51. 8% ), PR 31 cases (38. 3% ), NC 5 cases (6. 2% ) and PD 3 cases (3.7 % ). CR + PR was 73 cases (90. 1 % ). The effectiveness of treating 41 cases of benign hemangioma was: CR 38 cases (92. 7 % ) and PR 3 cases (7. 3 % ). One, three and five year survival rates of malig-nant tumour patients were 95 %, 65. 4 % and 37 %, respectively. While all the 41 patients with he-mangioma survived over five years after treatment.展开更多
291 cases of insomnia were treated with electro-acupuncture (EA). Among them, 139 cases were male and 152 female, aged between 16 - 60 years old. Selected acupoints were Taiyang (EX-HN 5), Neiguan (PC 6), Baihui (GV 2...291 cases of insomnia were treated with electro-acupuncture (EA). Among them, 139 cases were male and 152 female, aged between 16 - 60 years old. Selected acupoints were Taiyang (EX-HN 5), Neiguan (PC 6), Baihui (GV 20), Touwei (ST 8), Yintang (EX-HN 3) and were modified according to symptoms. Fengchi (GB 20) and Dazhui (GV 14) were added for the patient with indisposition or pain in nuchal region, Hegu (LI 4) for headache, and Shenmen (HT 7) for amnesia and dreaminess. EA was given with G6805 - Z Therapeutic Instrument, at continuous wave type, for 30 min, once a day. One therapeutic course consisted of 10 sessions. Any medicine was not administrated during the treatment. Results indicated that 216 cases (74. 2% ) were cured, 62 cases (21. 3 % ) were markedly effective. The total effective rate was 95. 5 %.展开更多
BACKGROUND: It has been found in recent years that STAT3 widely distributes in nervous system, including hippocampal CA1-3 region, dentate gyrus and cerebral neocortex, etc. Ischemic brain injury can cause the release...BACKGROUND: It has been found in recent years that STAT3 widely distributes in nervous system, including hippocampal CA1-3 region, dentate gyrus and cerebral neocortex, etc. Ischemic brain injury can cause the release of some cytokines and growth factors, while electro-acupuncture may have multi-level, multi-channel and multi-target protective and interventional effects on ischemic brain injury. OBJECTIVE: To observe the effects of electro-acupuncture on STAT3 expression and nuclear translocation in hippocampal CA1 region of rat models of brain ischemia/reperfusion. DESIGN: Randomized and controlled observation. SETTING: Staff Room of Acupuncture and Moxibustion, Department of Acupuncture and Bone Injury, Hubei College of Traditional Chinese Medicine; Tongji Medical College, Huazhong University of Science and Technology. MATERIALS: Seventy-two healthy SD rats, of clean degree and either gender, weighing (200±20) g, were provided by the Experimental Animal Center of Hubei College of Traditional Chinese Medicine. STAT3 monoclonal antibody was purchased from Santa Cruz Company, USA, and G-6805 electro-acupuncture instrument was purchased from Shanghai Medical Electronic Instruments Factory. METHODS: This experiment was carried out in the comprehensive laboratory of Department of Acupuncture and Bone Injury, Hubei College of Traditional Chinese Medicine between September 2005 and February 2006. Seventy-two rats were randomly divided into 4 groups: ① control group(n =6): Untouched. ② Sham-operation group (n =18): Artery was isolated, but without inserting thread bolt.③ Model group (n =24): Rat models of local brain ischemia/reperfusion were established with modified suture occlusion. ④Electro-acupuncture group (n =24): Dazhui and bilateral Neiguan points were selected for electro-acupuncture treatment. No. 28 acupuncture needle of 3.33 cm was used in the treatment. A G-6085 electro-acupuncture instrument with continuous wave, frequency of 120 times/min, intensity of 1 mA, 30 min/time, was used. Acupuncture was conducted firstly at ischemia/reperfusion 3 hours, then once every 12 hours. STAT3 positive nuclear translocation in hippocampal CA1 region of rats was observed with immunohistochemical method at 24, 48 and 72 hours after brain ishcemia/reperfusion, and then STAT3 positive cells were counted. MAIN OUTCOME MEASURES: STAT3 positive cells and nuclear translocation in hippocampal CA1 region of rats in each group. RESULTS: All the 72 rats were involved in the result analysis. ①In the control group and sham-operation group, STAT3 positive cells with light cytoplasm and nucleus were decreased , and nuclear translocation was not found. ② In the model group, STAT3 positive cells were mostly found in the cytoplasm of the hippocampal CA1 region at the ischemic side of rats after ischemia/reperfusion 24 hours. They were significantly more than those in the sham-operation group and control group [(18.00±2.68), (9.00±1.35), (8.00±1.22) cells/ mm2, P < 0.01], but cells with nuclear reaction were fewer; At ischemia/reperfusion 48 and 72 hours, STAT3 positive cells were increased, and they were significantly more than those of sham-operation group [(25.00±3.23), (35.00±3.52) cells/mm2, (13.00±1.93), (12.00±1.24) cells/mm2, P < 0.01]. Positive cells with nuclear reaction were found dark-stained. ③At ischemia/reperfusion 24, 48 and 72 hours, STAT3 positive cells were strongly expressed in hippocampal CA1 region at ischemic side of rats of electro-acupuncture group, and they were significantly more than those of model group [(25±3.52), (50±6.31), (75±8.09) cells/mm2, P < 0.01]. STAT3 positive cells were gradually enhanced with time, and considerable STAT3 nuclear positive reaction cells were found. CONCLUSION: Electro-acupuncture can activate STAT3 protein expression in hippocampal tissue of rats with local brain ischemia/reperfusion, promote STAT3 nuclear translocation and function its neuroprotective effect.展开更多
OBJECTIVE:To explore the prevention of urine retention after subabdominal surgery by electro-acupuncture Yin Ling Quan(SP 9),Zhao Hai(KI 6) acupoints with the assistance of subarachnoid acupoint anesthesia by observin...OBJECTIVE:To explore the prevention of urine retention after subabdominal surgery by electro-acupuncture Yin Ling Quan(SP 9),Zhao Hai(KI 6) acupoints with the assistance of subarachnoid acupoint anesthesia by observing the postoperative voiding time and urine volume of postoperative patients undergoing Electroacupuncture plus subarachnoid block anesthesia.METHODS:60 cases of preoperative expected for subarachnoid block surgery patients were randomly divided into two groups,the treatment group and the control group,with 30 cases in each group.The control group underwent routine subarachnoid anesthesia after surgery without acupuncture treatment group;The treatment group was treated with acupuncture bilateral Yin Ling Quan and Zhaohai acupoints 15 minutes before anesthesia,and then received unilateral electric anesthesia instrument treatment,the intensity of the patient was tolerable(generally 1.5 ~ 2.0 m A),and the routine subarachnoid block anesthesia was performed;The first urination time,urine volume,lower abdominal pain and assistant catheterization were recorded in the two groups.RESULTS:The first postoperative voiding time of the treatment group was significantly earlier compared with that of the control group,there was significant difference(P<0.05);the amount of urine was significantly more than that of the control group in reservation time,there was significant difference(P<0.05);the abdominal pain was light in the treatment group,there was significant difference(P<0.05);the cases of assisted catheterization of the control group were significantly more thanthose of the treatment group,there was significant difference(P< 0.05).CONCLUSION:Electro-acupuncture Yin Ling Quan(SP 9),Zhao Hai(KI 6) acupoints with the assistance of subarachnoid acupoint anesthesia could significantly reduce the urine retention after subabdominal surgery in postoperative patients with ubarachnoid block anesthesiau,accelerate the recovery of bladder function and promote early postoperative urination in addition to relieving the abdominal symptoms after operation.展开更多
基金This study was supported by grants from the National Basic Research Program(No.2011CB505203)the National Natural Science Foundation of China(No.81603652,No.81874388).
文摘Functional constipation(FC)is a chronic discasc that significantly afcts the life quality of patients.Acupuncture has been used for the treatment of FC for many years,but its effectiveness has not been scientifcally assessed.The present study aimed to evaluate the efficacy of electro-acupuncture(EA)in relieving the symptoms,mental states and quality of life(QOL)of FC patients.A total of 96 FC patients were randomly allocated into EA,mosapride&sham EA group(MS)and mosapride control group(MC).In the EA group,patients were treated with 16 sessions of needling at Quchi(LI1I)and Shangjuxu(ST37)bilaterally,5 times a week in the first 2 weeks,and 3 times a.week in the last 2 weeks.In the MC group,patients were treated with 5 mg mosapride citrate three times a day for 4 weeks.In the MS group,patients underwent sham EA and the same mosapride citrate treatment as in the MC group.The primary outcome was the number of weekly spontaneous bowel movements(SBMs).The secondary outcomes included stool consistency,intensity of defecating difficulty,36-Item Short-Form Health Survey(SF-36),Self-rating Anxiety Scale(SAS),Self-rating Depression Scale(SDS),and the validated Patient Assessment of Constipation-Quality of Life(PAC-QOL).The results showed that as compared with the baseline,EA significantly improved the weekly SBMs,stool consistency and intensity of defecating difficulty(P<0.05).It also partly ameliorated the PAC-QOL,SF-36,SDS and SAS scores when compared with MC or MS group(P<0.05).However,no significant difference was observed between MS and MC groups in bowel function outcomes and QOL scores.It was concluded that EA could effectivcly improve bowel function,mental states and QOL of FC patients.
基金the National Natural Science Foundation of China,No.30371808the Natural Science Foundation of Guangdong Province,No.5009688
文摘BACKGROUND:Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However,there are few reports on the influence of electro-acupuncture at the Du meridian on neural stem cell proliferation. OBJECTIVE:To observe the influence of electro-acupuncture at Ren and Du meridians on neural stem cell proliferation in the subventricular zone and altered signal transduction in cerebral ischemia rats. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Human Anatomy,Medical College of Sun Yat-sen University from May 2006 to February 2008. MATERIALS:Mouse anti-rat bromodeoxyuridine (BrdU) monoclonal antibody was provided by Sigma,USA; mouse anti-rat nestin monoclonal antibody and extracellular signal-regulated protein kinase (ERK) specific inhibitor PD98059 were provided by Calbiochem,Germany; acupuncture needle was provided by Suzhou Acupuncture Supplies,China. METHODS:A total of 126 rats were randomly assigned to four groups:model (n = 36),Du meridian (n = 36),Ren/Du meridian (n = 36),and Ren/Du meridian + PD98059 (n = 18). Rats in the Ren /Du meridian + PD98059 group were observed on days 7 (n = 6) and 14 (n = 12) after cerebral ischemia injury. Rats in the model,Du meridian,and Ren/Du meridian groups were observed on days 7,14,and 28 after cerebral ischemia injury,with 12 rats per group at each time point. Thread occlusion was used to establish middle cerebral artery occlusion models. Electro-acupuncture was performed at Renzhong (DU 26) and Baihui (DU 20) acupoints in the Du meridian group,as well as Chengjiang (RN 24),Guanyuan (RN 4),Renzhong,and Baihui acupoints in the Ren/Du meridian and Ren/Du meridian + PD98059 groups 2 days after model establishment. In addition,electro-acupuncture stimulation with disperse-dense waves was performed,with 30 Hz disperse wave,100 Hz dense wave,and 5 V intensity for 20 minutes. Rats in the Ren/Du meridian + PD98059 group were treated with 0.2 μg PD98059 injection into the subventricular zone,2 μL per rat. Rats in the model group were not treated with electro-acupuncture. MAIN OUTCOME MEASURES:BrdU/nestin immunofluorescent staining was used to detect proliferating neural stem cells in the subventricular zone of cerebral ischemia rats; Western blot was used to determine phosphorylated ERK1 and 2 (pERK1/2) expression in the subventricular zone. RESULTS:On days 14 and 28 after cerebral ischemia,there were significantly more BrdU-positive and BrdU/nestin-positive cells in the Ren /Du meridian group compared with the Du meridian group (P < 0.05). PD98059 decreased the number of BrdU-positive and BrdU/nestin-positive cells induced by electro-acupuncture at the Ren and Du meridians (P < 0.05). On days 7,14,and 28 after treatment,pERK1/2 expression was significantly greater in the Du meridian and Ren/Du meridian groups compared with the model group (P < 0.05). The promoting effect of electro-acupuncture at Ren and Du meridians on ERK1/2 phosphorylation was superior to electro-acupuncture at the Du meridian alone on day 14 after model induction (P < 0.05). However,PD98059 completely abolished the promoting effect of electro-acupuncture at Ren/Du meridians on pERK1/2 expression (P < 0.05). CONCLUSION:Electro-acupuncture at Ren and Du meridians increased proliferation of subventricular zone neural stem cells,which was related to activation of the ERK pathway in a rat model of cerebral ischemia injury.
基金supported by a grant from the National Natural Science Foundation of China (No.30640010)
文摘This study examined the effect of electro-acupuncture (EA) combined with transcranial magnetic stimulation (TMS) therapy at different time windows on learning and memory ability of rats with cerebral infarction and the underlying mechanism.Two hundred SD rats were randomly divided into four groups:normal group,sham-operated group,model group and EA+TMS group,and each group was then divided into five sub-groups in terms of the different time to start treatment post operation:6,12,24,48 and 72 h.Cerebral infarction models were established in the model and the EA+TMS groups by left middle cerebral artery occlusion/reperfusion (MCAO/R).After treatment for 14 d,the Morris water maze test was applied to examine the spatial learning and memory abilities of rats.In infarcted area,the expression of caspase-3 was immunohistochemically detected,and real-time fluorescent quantitative PCR was used to measure the expression of Bcl-2 mRNA.The results showed that in EA+TMS group compared with model group at the same treatment time windows,the escape latency was substantially shortened,the expression of caspase-3 was considerably decreased and the expression level of Bcl-2 mRNA significantly increased (P<0.05).In the EA+TMS sub-groups,the escape latency was shortest,the expression level of caspase-3 lowest,and the expression level of Bcl-2 mRNA highest at the treatment time window of 24 h.It was concluded that EA combined with TMS can promote neurological function of rats with cerebral infarction by increasing the expression level of Bcl-2 mRNA and decreasing the expression of caspase-3.The best time window is 24 h after perfusion treatment to ischemia.
基金Supported by National Basic Research Program of China(973 Program)No.2009CB522900 and No.2015CB554501National Natural Science Foundation of China,No.30973784
文摘AIM To observe whether there are differences in the effects of electro-acupuncture(EA) and moxibustion(Mox) in rats with visceral hypersensitivity. METHODS EA at 1 m A and 3 m A and Mox at 43?℃ and 46?℃ were applied to the Shangjuxu(ST37, bilateral) acupoints in model rats with visceral hypersensitivity. Responses of wide dynamic range neurons in dorsal horns of the spinal cord were observed through the extracellular recordings. Mast cells(MC) activity in the colons of rats were assessed, and 5-hydroxytryptamine(5-HT), 5-hydroxytryptamine 3 receptor(5-HT3R) and 5-HT4Rexpressions in the colons were measured.RESULTS Compared with normal control group, responses of wide dynamic range neurons in the dorsal horn of the spinal cord were increased in the EA at 1 m A and 3 m A groups(1 m A: 0.84 ± 0.74 vs 2.73 ± 0.65, P < 0.001; 3 m A: 1.91 ± 1.48 vs 6.44 ± 1.26, P < 0.001) and Mox at 43?℃ and 46?℃ groups(43?℃: 1.76 ± 0.81 vs 4.14 ± 1.83, P = 0.001; 46?℃: 5.19 ± 2.03 vs 7.91 ± 2.27, P = 0.01). MC degranulation rates and the expression of 5-HT, 5-HT3 R and 5-HT4 R in the colon of Mox 46?℃ group were decreased compared with model group(MC degranulation rates: 0.47 ± 0.56 vs 0.28 ± 0.78, P < 0.001; 5-HT: 1.42 ± 0.65 vs 7.38 ± 1.12, P < 0.001; 5-HT3R: 6.62 ± 0.77 vs 2.86 ± 0.88, P < 0.001; 5-HT4R: 4.62 ± 0.65 vs 2.22 ± 0.97, P < 0.001). CONCLUSION The analgesic effects of Mox at 46?℃ are greater than those of Mox at 43?℃, EA 1 m A and EA 3 m A.
基金Science and Technology Plan Program of Social Development of Guangdong Science and Technology Department in 2007,No.73127
文摘BACKGROUND: Spleen deficiency in traditional Chinese medicine refers to the functional disorder of spleen,pancreas,intestines,and nervous system in modern medicine.OBJECTIVE: To test whether electro-acupuncture could alter basic fibroblast growth factor (bFGF) protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.DESIGN,TIME AND SETTING: The randomized,controlled,in vivo animal experiment was performed at the National Level-B Laboratory of Clinical Cell Molecule and Biology in Shenzhen Hospital of Traditional Chinese Medicine,between March and November in 2008.MATERIALS: Reserpine injection was produced by Guangdong Bangmin Pharmaceutical Co.Rhubarb extract granule preparation was produced by Guangdong Yifang Pharmaceutical.Huanqiu Brand sterile acupuncture pin was provided by Suzhou Acupuncture Supplies,China.Huatuo Brand electroacupuncture instrument (type SDZ-II) was purchased from Suzhou Medical Appliance Factory,China.METHODS: A total of 96 male Sprague Dawley rats were randomly assigned to control (n = 32) and induction (n = 64) groups.Spleen deficiency was induced via intraperitoneal injection of reserpine and intragastric administration of rhubarb.The successful models were randomized into two groups: model and electro-acupuncture,with 32 rats in each group.Elec-tro-acupuncture was administered at Zusanli (ST 36) and Sanyinjiao (SP 6) acupoints using a condensation wave and rarefaction (condensation wave 15 Hz) at a strength of 6–15 V for 20 minutes,once per day.The appearance of a slight shiver in the corresponding locus was taken as the standard.According to electro-acupuncture time points,each group was assigned to four subgroups at 7,14,28,and 49 days,respectively,with eight rats in each subgroup.Immunohistochemical staining,image analysis,and reverse-transcription polymerase chain reaction were performed at different time points.MAIN OUTCOME MEASURES: bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.RESULTS: After 7 days of electro-acupuncture therapy,bFGF protein and mRNA expression significantly increased compared with the model and control groups (P < 0.05).After 14 days,bFGF protein and mRNA expression decreased until 28 days,where levels were then equal to the model group and greater than the control group (P < 0.05).After 49 days,the above indices remained increased in the electro-acupuncture group compared to the model and control groups (P < 0.05).CONCLUSION: Continuous electro-acupuncture maintained a high level of bFGF protein and mRNA expression in the hippocampal dentate gyrus of spleen deficiency rats.
基金Supported by National Natural Science Foundation of China
文摘The influence of electro-acupuncture at Neiguan (PC 6)and five shu points of twelve regular meridians at different time on systolic time interval (ST I) of normal young adultswere studied with 6×6 Latin square design in this work.In 12 groups according to 12 regular merldlans,the STIs were measured respectively just before and after electro-acupuncture on Neiguan andfive shu points at 00:00,04:00,08:00,12:00,16:00 and 20:00 of a day.It was found thatexcept the PEPI and PEPI/LVETI ratio among Shenmen(HT 7),Shaochong(HT 9)and Neiguan,Guanchong(TE)and Yemen(TE 2)points,the effects of electro-acupuncture at Neiguan pointand five shu points of twelve regular meridians on the indices of STI of normal young adults wereshowing no slgnificant differences.There also was no difference in different sex.But the indices ofSTI were changed when electroacupuncture on the different points of different meridians at differenttime.
基金the National Natural Science Foundation of China(81573773 and 81774110)Self-determined Project of Beijing University of Chinese Medicine(2017-JYB-JS-004).
文摘Objective:To summarize the current clinical evidence related to the therapeutic effects and safety of adjuvant scalp electro-acupuncture (SEA) treatment for Parkinson's disease in China.Methods:Following the PRISMA statement,seven electronic databases were searched to retrieve randomized controlled clinical trials that used SEA combined with medication as the treatment intervention,and medication as the control.RevMan 5.3 was used to analyze outcomes,including the Unified Parkinson's Disease Rating Scale (UPDRS),Webster scale,effectiveness rate,and UPDRS III.Results:Nine randomized controlled trials,with certain methodological flaws and risks of bias,were included that involved 474 participants.SEA combined with medication was more effective than medication alone in overall therapeutic effects,as evidenced by total UPDRS scores (mean difference (MD):7.15,95% confidence interval [CI] 0.24 to 14.07,P =.04),Webster scores (MD:1.60,95% CI 0.20 to 2.99,P =.03),and effectiveness rate (risk ratio:1.35,95% CI 1.19 to 1.54,P <.001).In addition,there was significant improvement in pooled motor function results after adjuvant SEA treatment compared with medication alone (MD:5.75,95% Cl 4.18 to 7.32,P <.001).Conclusion:The combination of SEA and medication may be a promising intervention for patients with Parkinson's disease,especially to improve motor function.However,results were inconclusive,and additional studies with rigorous experimental design and larger sample sizes are needed to verify these results.
基金the National Natural Science Foundation of China(81473774).
文摘Objective:To determine whether electro-acupuncture (EA) therapy could improve the cognitive functions of amyloid precursor protein Swedish mutation (APPswe)/presenilin 1 deleted in exon 9 (PS1dE9) mice and examine whether EA treatment could attenuate neuroinflammation by targeting the toll-like receptor 4 (TLR4)/myeloid differentiation primary response factor 88 (MyD88) signaling pathway.Methods:Twenty-seven double transgenic APPswe/PS1dE9 mice were randomly allocated into three groups:an Alzheimer's disease model group (AD group),a medication group (M group) and an EA treatment group (EA group).Each group contained nine mice,and nine wild-type mice were used in a normal group (N group).The animals in the M group were treated with oral administrations of 0.92 mg/kg donepezil hydrochloride for 15 days.For animals in the EA group,EA treatments were used on the Yintang (GV 29) and Baihui (GV 20) acupoints for 20 minutes,and the Shuigou (GV 26) acupoint was pricked without needle retention following EA treatments.Following treatments,the spatial learning and memory of the mice were measured using the Morris water maze test.The expression levels of TLR4,MyD88,nuclear factor kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) were analyzed by immunohistochemical staining and western blot.Results:The escape latencies of the M and EA groups were significantly lower than those of the AD group (vs M,P =.002;vs EA,P <.001).Moreover,compared with the AD group,the numbers of platform crossings was higher (vs M,P =.038;vs EA,P =.008) and the latency time for target quadrants was longer (vs M,P =.002;vs EA,P =.001) in the M and EA groups (P <.05).Furthermore,in the M and EA groups,the expression levels of TLR4,MyD88,NF-κB and iNOS decreased significantly compared with those of the AD group (all P <.01).Conclusion:EA treatment enhanced the memory and learning abilities of APPswe/PS1dE9 mice by regulating the TLR4/MyD88 inflammatory signaling pathway.
基金financially supported by National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2007BAI10B01-027)Project of Chinese Medicine Research Fund of Shanghai Municipal Health Bureau (No. 20134358)
文摘OBJECTIVE: To investigate that whether electro-acupuncture(EA) precondition can reduce the incidence of postoperative cognitive dysfunction(POCD) following knee replacement and its safety in elderly. METHODS: A total of 60 participants met the inclusion criteria were enrolled in a randomized controlled trial with the ratio of 1:1, with 30 cases in the treatment group and 30 cases in the control group. The participants in the treatment group were provided with realEA therapy whereas participants in control group were provided with placebo-EA therapy. Interventions were offered 5 days prior to the surgery, once daily, and for a total of 5 days. The scores of Mini-Mental State Examination(MMSE), and contents of serumal inflammatory cytokines including interleukin 1β(IL-1β) and tumor necrosis factor-α(TNF-α) were observed at 24 hours prior-and posterior-to the surgery respectively for assessing the incidence of POCD among patients. Meanwhile, adverse effects were monitored and recorded. RESULTS:(1) After surgery, both treatment group and control group showed a significant decrease in MMSE global scores(P < 0.001, < 0.001, respectively), and the score in control group decreased more significantly than that in treatment group(P < 0.05);(2) Contents of serumal IL-1β and TNF-α were significantly increased in both groups after 24 hours posterior to the surgery(P < 0.001), and the contents in control group increased more significantly than that in treatment group(P < 0.001);(3) After surgery, the incidence of POCD was 20% in treatment group versus 36.67% in control group. There was no statistical difference between 2 groups(P > 0.05);(4) No serious adverse events were reported in this trial, except 1 patient from treatment group had a slight hematoma after receiving acupuncture. CONCLUSION: EA precondition might reduce cognitive impairments after 24 hours posterior to knee replacement surgery in elderly through inhibiting expression of inflammatory cytokines, including both IL-1β and TNF-α. However, there is insufficient evidence to support that EA precondition could reduce incidence of POCD.
文摘Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment;the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.
文摘44 cats were used in this experiment. The spinal cords of 40 animals were injuredat about L<sub>1</sub> level by using Allen’s method （400g/cm） and randomly divided into 2 groups: A)electro-acupuncture treatment group （n=20） and B) control group（n=20）. 2 weeks after spinalcord injury, 80% of animals in the treatment group were survival and 45% of animals in the con-trol group were survival. There was significant difference between these two groups （p【0. 05）.The motor evoked potential was recorded two weeks after spinal cord injury. The latency of theshort latency peak was 15. 61±4. 98 mS and the amplitude was 10. 61 0. 59 mV in the treatmentgroup; and 21. 7±5. 02 mS and 0. 56±0. 32 mV in the control group. Both latency and amplitudewere significant different between the two groups （P【0. 05, P【0. 05）. The Anti-NF-H （neuro-filament） was determined at 2 and 4 weeks respectively after spinal cord injury. The more NF-Hpositive labeling fibers were found in the treatment group than in the control group （P【0. 01 ）.
文摘In this study, in situ hybridization histochemistry technique was used to observe the effect of electro-acupuncture (EA) on glutamate transporter mRNA expression following focal cerebral ischemia. We observed that glutamate transporter mRNA expression decreased significantly in the area penumbra of cerebral cortex at 12h following focal cerebral ischemia. EA did not change glutamate transporter mRNA expression in normal rat brain but prevented the decrease of glutamate transporter mRNA expression in the area penumbra of cerebral cortex following focal cerebral ischemia. The results suggest that the protective effect of EA on ischemic neuronal death may bhe related to the increase of uptake of glutamate by its transporter.
基金supported by the Ministry of Science and Technology of China (2016YFA0400902)the National Science Foundation of China (11575278, 21675167, 81690263, 21227804, 21505148, and U1632125)+2 种基金the Project of State Key Laboratory of Radiation Medicine and Protection, Soochow University (GZK1201813)the Key Research Program of Frontier Sciences (QYZDJ-SSW-SLH031)the Open Large Infrastructure Research of Chinese Academy of Sciences (CAS) and Youth Innovation Promotion Association, CAS (2012205 and 2016236)
文摘Rett syndrome is a progressive neurodevelopmental disorder that lacks effective treatments.Although deep-brain stimulation can alleviate some symptoms in Rett model mice,this interventional manipula-tion requires deliberate surgical operations.Here,we report that electro-acupuncture stimulation(EAS)can ameliorate symptoms of an Mecp2-knockout rat model of Rett syndrome from the remote acupoints Baihui(GV 20),Yongquan(KI 1),and Shenmen(HT 7).We find that EAS not only prolongs the survival time of Rett rats,but also improves their behavior ability,including locomotion,motor coordination,and social interaction.Neural activation was observed in the substantia nigra of the midbrain,corpus striatum,and cerebral cortex of wild-type and Rett model rats,as reflected by the increased expression of the c-Fos protein.Hence,EAS provides a potential promising therapeutic tool for treating neurodevel-opmental diseases.
文摘119 patients with gastroduodenal diseases were observed,clinically and experi-mentally,on the effect of electro-acupuncture at the auricular acupoint"Gastric area" on gastrogram,and the effect was compared with that of Heart area and Ear tip points.There was a significant differ-ence in the amplitudes and frequencies of gastrogram of patients between pre-and post-acupuncture(P【0.01),with good diphasic regulation;the effect of acupuncturing at"Gastric area" is the mostsignificant as compared with that at "Heart" and "Ear tip".The experiment demonstrates that thereis a relationship between the "Gastric area" and stomach,and indicates that there is relative specificityof "Gastric Area" to stomach.This provides the experimental foundation for the auricular acupointtherapy in clinic.
文摘Electro- acupuncture (EA) applied to acupoints of GV channel suppresses spike- wave discharges(SW) of PTZ-induced petit mal epilepsy in rats. EA decreases cortical excitability and increases its inhibitory process. Electric stimulation of subcortical nuclei: caudate n., cuneiform n. of midbrain RF, substantia nigra and superior colliculus of rats with petit mal epilepsy can suppress SW, and EA effect becomes low after destruction of the latter at two sites. Stimulation and destruction applied to reticular n. of thalamus elicit reverse effect. Microinjection of drugs affecting neurotransmitters: HC3, haloperidal, PCA and 3-MP into certain parts of brain leads to prolonged SW and reduced EA effect. Injection of muscimol shortens SW duration and reinforces EA effect, while baclofen induces reverse result. AP5 causes shortening of SW duration. The contents of GABA, glycine and taurine are increased markedly in sensory-motor area and optic area of the cortex after EA, while glutamate is decreased. The GABA contents of thalamic reticular n. and reticular part of substantia nigra are also obviously elevated.
文摘Nineteen patients suffering from common rnental diseases were treated by computer-ized electrode and electro-acupuncture. lt was revealed that 66. 7% of 9 cases treated by electrodewere rnarkedly effective without significant difference to the effective rate of 1O cases treated throughelectro-acupuncture (P】0. 05). The score of self anxiety scale in the patients treated by electrodewas markedly reduced, while the score of sleep state scale was markedly decreased in those patientstreated by electro-acupuncture. There was no side-effect in the surface electrode therapy l the patientswere without fear, and accepted it with m0re pleasure.
文摘Electrochemical therapy, ECT, has been used to treat 122 cases of various kinds of tu-mour, among them, 81 cases were malignant tumours (32 cases facial, 18 cases subaural, 16 cases labial, 15 cases tongue), and the other 41 cases were bening tumours (cavermous hemangioma at facial region 23 cases and in oral cavity 18 cases). Pathological examination of malignant tumours revealed that there were 63 cases of squamous epithelial cancers and 18 cases of adenocarcinoma. Cavermous he-mangioma is benign tumour and congenital. Special designed and made platinum needles were inserted into the tumours and the needles were connected to an instrument protucing a direct current for treat-ment. Voltage used was usually 8 volts with current 60 - 80 mA. The quantity elecricity applied was 100 coulombs per 1 cm in diameter of the tumour. The effectiveness of treating 81 cases malignant tu-mours was: CR 42 cases (51. 8% ), PR 31 cases (38. 3% ), NC 5 cases (6. 2% ) and PD 3 cases (3.7 % ). CR + PR was 73 cases (90. 1 % ). The effectiveness of treating 41 cases of benign hemangioma was: CR 38 cases (92. 7 % ) and PR 3 cases (7. 3 % ). One, three and five year survival rates of malig-nant tumour patients were 95 %, 65. 4 % and 37 %, respectively. While all the 41 patients with he-mangioma survived over five years after treatment.
文摘291 cases of insomnia were treated with electro-acupuncture (EA). Among them, 139 cases were male and 152 female, aged between 16 - 60 years old. Selected acupoints were Taiyang (EX-HN 5), Neiguan (PC 6), Baihui (GV 20), Touwei (ST 8), Yintang (EX-HN 3) and were modified according to symptoms. Fengchi (GB 20) and Dazhui (GV 14) were added for the patient with indisposition or pain in nuchal region, Hegu (LI 4) for headache, and Shenmen (HT 7) for amnesia and dreaminess. EA was given with G6805 - Z Therapeutic Instrument, at continuous wave type, for 30 min, once a day. One therapeutic course consisted of 10 sessions. Any medicine was not administrated during the treatment. Results indicated that 216 cases (74. 2% ) were cured, 62 cases (21. 3 % ) were markedly effective. The total effective rate was 95. 5 %.
基金the Natural Science Foundation of Hubei Province,No.2003ABA154
文摘BACKGROUND: It has been found in recent years that STAT3 widely distributes in nervous system, including hippocampal CA1-3 region, dentate gyrus and cerebral neocortex, etc. Ischemic brain injury can cause the release of some cytokines and growth factors, while electro-acupuncture may have multi-level, multi-channel and multi-target protective and interventional effects on ischemic brain injury. OBJECTIVE: To observe the effects of electro-acupuncture on STAT3 expression and nuclear translocation in hippocampal CA1 region of rat models of brain ischemia/reperfusion. DESIGN: Randomized and controlled observation. SETTING: Staff Room of Acupuncture and Moxibustion, Department of Acupuncture and Bone Injury, Hubei College of Traditional Chinese Medicine; Tongji Medical College, Huazhong University of Science and Technology. MATERIALS: Seventy-two healthy SD rats, of clean degree and either gender, weighing (200±20) g, were provided by the Experimental Animal Center of Hubei College of Traditional Chinese Medicine. STAT3 monoclonal antibody was purchased from Santa Cruz Company, USA, and G-6805 electro-acupuncture instrument was purchased from Shanghai Medical Electronic Instruments Factory. METHODS: This experiment was carried out in the comprehensive laboratory of Department of Acupuncture and Bone Injury, Hubei College of Traditional Chinese Medicine between September 2005 and February 2006. Seventy-two rats were randomly divided into 4 groups: ① control group(n =6): Untouched. ② Sham-operation group (n =18): Artery was isolated, but without inserting thread bolt.③ Model group (n =24): Rat models of local brain ischemia/reperfusion were established with modified suture occlusion. ④Electro-acupuncture group (n =24): Dazhui and bilateral Neiguan points were selected for electro-acupuncture treatment. No. 28 acupuncture needle of 3.33 cm was used in the treatment. A G-6085 electro-acupuncture instrument with continuous wave, frequency of 120 times/min, intensity of 1 mA, 30 min/time, was used. Acupuncture was conducted firstly at ischemia/reperfusion 3 hours, then once every 12 hours. STAT3 positive nuclear translocation in hippocampal CA1 region of rats was observed with immunohistochemical method at 24, 48 and 72 hours after brain ishcemia/reperfusion, and then STAT3 positive cells were counted. MAIN OUTCOME MEASURES: STAT3 positive cells and nuclear translocation in hippocampal CA1 region of rats in each group. RESULTS: All the 72 rats were involved in the result analysis. ①In the control group and sham-operation group, STAT3 positive cells with light cytoplasm and nucleus were decreased , and nuclear translocation was not found. ② In the model group, STAT3 positive cells were mostly found in the cytoplasm of the hippocampal CA1 region at the ischemic side of rats after ischemia/reperfusion 24 hours. They were significantly more than those in the sham-operation group and control group [(18.00±2.68), (9.00±1.35), (8.00±1.22) cells/ mm2, P < 0.01], but cells with nuclear reaction were fewer; At ischemia/reperfusion 48 and 72 hours, STAT3 positive cells were increased, and they were significantly more than those of sham-operation group [(25.00±3.23), (35.00±3.52) cells/mm2, (13.00±1.93), (12.00±1.24) cells/mm2, P < 0.01]. Positive cells with nuclear reaction were found dark-stained. ③At ischemia/reperfusion 24, 48 and 72 hours, STAT3 positive cells were strongly expressed in hippocampal CA1 region at ischemic side of rats of electro-acupuncture group, and they were significantly more than those of model group [(25±3.52), (50±6.31), (75±8.09) cells/mm2, P < 0.01]. STAT3 positive cells were gradually enhanced with time, and considerable STAT3 nuclear positive reaction cells were found. CONCLUSION: Electro-acupuncture can activate STAT3 protein expression in hippocampal tissue of rats with local brain ischemia/reperfusion, promote STAT3 nuclear translocation and function its neuroprotective effect.
文摘OBJECTIVE:To explore the prevention of urine retention after subabdominal surgery by electro-acupuncture Yin Ling Quan(SP 9),Zhao Hai(KI 6) acupoints with the assistance of subarachnoid acupoint anesthesia by observing the postoperative voiding time and urine volume of postoperative patients undergoing Electroacupuncture plus subarachnoid block anesthesia.METHODS:60 cases of preoperative expected for subarachnoid block surgery patients were randomly divided into two groups,the treatment group and the control group,with 30 cases in each group.The control group underwent routine subarachnoid anesthesia after surgery without acupuncture treatment group;The treatment group was treated with acupuncture bilateral Yin Ling Quan and Zhaohai acupoints 15 minutes before anesthesia,and then received unilateral electric anesthesia instrument treatment,the intensity of the patient was tolerable(generally 1.5 ~ 2.0 m A),and the routine subarachnoid block anesthesia was performed;The first urination time,urine volume,lower abdominal pain and assistant catheterization were recorded in the two groups.RESULTS:The first postoperative voiding time of the treatment group was significantly earlier compared with that of the control group,there was significant difference(P<0.05);the amount of urine was significantly more than that of the control group in reservation time,there was significant difference(P<0.05);the abdominal pain was light in the treatment group,there was significant difference(P<0.05);the cases of assisted catheterization of the control group were significantly more thanthose of the treatment group,there was significant difference(P< 0.05).CONCLUSION:Electro-acupuncture Yin Ling Quan(SP 9),Zhao Hai(KI 6) acupoints with the assistance of subarachnoid acupoint anesthesia could significantly reduce the urine retention after subabdominal surgery in postoperative patients with ubarachnoid block anesthesiau,accelerate the recovery of bladder function and promote early postoperative urination in addition to relieving the abdominal symptoms after operation.
